FastQCFastQC Report
Wed 25 May 2016
SRR1294693_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294693_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2631359
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC140970.5357307763782897No Hit
CCCATGTACTCTGCGTTGATACCAC137570.522809696434428No Hit
GTATCAACGCAGAGTACTTTTTTTT111440.4235073967482202No Hit
GGTATCAACGCAGAGTACTTTTTTT86540.32887948774758596No Hit
TATCAACGCAGAGTACTTTTTTTTT77760.2955126989513784No Hit
GAGTACATGGGAAGCAGTGGTATCA72650.27609307585928033No Hit
CATGTACTCTGCGTTGATACCACTG47840.18180719544539534No Hit
ACGCAGAGTACATGGGAAGCAGTGG47570.1807811096851475No Hit
ACGCAGAGTACTTTTTTTTTTTTTT46320.17603071264696304No Hit
GCGTTGATACCACTGCTTCCCATGT44850.17044424573005812No Hit
GTATCAACGCAGAGTACATGGGAAG42910.16307162952679585No Hit
TATCAACGCAGAGTACATGGGAAGC36210.1376095014021272No Hit
ACTCTGCGTTGATACCACTGCTTCC32350.1229402753482136No Hit
GGTATCAACGCAGAGTACATGGGAA31710.12050807206466316No Hit
GCTTCCCATGTACTCTGCGTTGATA31050.11799986242850179No Hit
GTGGTATCAACGCAGAGTACATGGG30550.116099703613228No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG400.00523655514.2679175
TATTCCG853.8911985E-612.3095745
ATACCGT701.0962962E-412.2084966
TCGTCTA550.00308077512.08494714
TGGACCG802.8314846E-511.8899315
GCTCGTT658.0599374E-411.68654311
CGCCCTA752.0843817E-411.3943816
TAGACAG3700.011.31150155
CCGGTAC600.005822777711.0972683
ATGCGCC600.005899107511.07786811
TGTCCCG951.3685358E-510.99457611
CGGTACT909.399391E-510.5688284
GGACCGC909.5657735E-510.5505526
CCAGAAC3450.010.4769253
TAGACTG4200.010.1913695
TAGTCCG1054.0508294E-59.9648945
TTCCGTA1054.1294617E-59.9474747
TAAACAG4700.09.7143265
CCACGAG900.00110132229.5119453
TAGGACG800.00447062459.5119444
Produced by FastQC (version 0.11.5)