FastQCFastQC Report
Wed 25 May 2016
SRR1294692_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294692_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5351431
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC2389644.465422426263181No Hit
CCCATGTACTCTGCGTTGATACCAC2317974.331495631729158No Hit
GAGTACATGGGAAGCAGTGGTATCA1002011.8724150605697802No Hit
CATGTACTCTGCGTTGATACCACTG806171.5064568710686919No Hit
GCGTTGATACCACTGCTTCCCATGT758111.4166491168437003No Hit
GTATCAACGCAGAGTACATGGGAAG628611.1746577691088607No Hit
ACGCAGAGTACATGGGAAGCAGTGG586431.0958377301323703No Hit
GCTTCCCATGTACTCTGCGTTGATA508670.9505308019481145No Hit
TATCAACGCAGAGTACATGGGAAGC505530.9446632125126906No Hit
ACTCTGCGTTGATACCACTGCTTCC493290.9217908256688725No Hit
GGTATCAACGCAGAGTACATGGGAA393640.7355789507516775No Hit
CAGTGGTATCAACGCAGAGTACATG316490.5914119045915008No Hit
GTGGTATCAACGCAGAGTACATGGG311580.5822367886271914No Hit
ATACCACTGCTTCCCATGTACTCTG273090.5103121015668519No Hit
GTACTCTGCGTTGATACCACTGCTT262640.49078461443303667No Hit
GCAGTGGTATCAACGCAGAGTACAT258880.48375845638297493No Hit
CATGGGAAGCAGTGGTATCAACGCA228150.42633456359616706No Hit
CTGCTTCCCATGTACTCTGCGTTGA227750.4255870999738201No Hit
GGGAAGCAGTGGTATCAACGCAGAG226760.4237371275085113No Hit
GTACATGGTAAGCAGTGGTATCAAC223040.41678571582068424No Hit
TACCACTGCTTCCCATGTACTCTGC211470.3951653305442974No Hit
ACATGGGAAGCAGTGGTATCAACGC205950.3848503325559089No Hit
GATACCACTGCTTCCCATGTACTCT199730.3732272732284131No Hit
CTTCCCATGTACTCTGCGTTGATAC189950.3549517876620291No Hit
CCATGTACTCTGCGTTGATACCACT186640.3487665261871077No Hit
GCAGAGTACATGGGAAGCAGTGGTA186300.3481311821081128No Hit
GTTGATACCACTGCTTCCCATGTAC177070.33088345902245586No Hit
AAGCAGTGGTATCAACGCAGAGTAC169910.31750386018244464No Hit
CCACTGCTTCCCATGTACTCTGCGT165690.30961811896668384No Hit
ACCATGTACTCTGCGTTGATACCAC160670.3002374505062291No Hit
ATGGGAAGCAGTGGTATCAACGCAG150460.28115844154582204No Hit
GTACATGGGTAAGCAGTGGTATCAA148400.27730900389073504No Hit
TTCCCATGTACTCTGCGTTGATACC139750.26114510305748123No Hit
TGATACCACTGCTTCCCATGTACTC135490.2531846154794858No Hit
ATCAACGCAGAGTACATGGGAAGCA116750.21816594477252907No Hit
GGTAAGCAGTGGTATCAACGCAGAG112420.21007465106062284No Hit
CCCCATGTACTCTGCGTTGATACCA101260.1892204159971417No Hit
AAAAAGTACTCTGCGTTGATACCAC75230.14057922077291102No Hit
GCTTACCATGTACTCTGCGTTGATA73250.1368792758422934No Hit
ATTCCATTCCATTCCATTCCATTCC71740.13405760066793349No Hit
GGAAGCAGTGGTATCAACGCAGAGT70600.13192732934424456No Hit
TCCATGTACTCTGCGTTGATACCAC70200.13117986572189758No Hit
GAGTACATGGTAAGCAGTGGTATCA69400.12968493847720358No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA67580.12628397899552474No Hit
GAATGGAATGGAATGGAATGGAATG63670.11897752208708287No Hit
ACTCTGCGTTGATACCACTGCTTAC58690.10967159998886279No Hit
CTGCGTTGATACCACTGCTTCCCAT58400.10912968886266122No Hit
CTCTGCGTTGATACCACTGCTTCCC58370.1090736290909852No Hit
GTACATGGAAAGCAGTGGTATCAAC57180.1068499248145029No Hit
TCCCATGTACTCTGCGTTGATACCA55150.1030565469310919No Hit
TGGGAAGCAGTGGTATCAACGCAGA54160.10120657446578307No Hit
GCGTTGATACCACTGCTTACCATGT53670.10029093152840801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAAA5400.012.665952
TACGACC701.093296E-412.2125684
GTACAAA10800.011.7011471
GTCGTCG752.0835717E-411.39530857
CGTATAC600.0058829511.0822923
ACGCAAA6700.010.6362121
TCGTCGT909.563958E-510.5512118
CGTCGTA909.563958E-510.5512119
AAAAAGT14950.010.3596941
AAAAGTA15150.010.2832462
GTCCTAG3150.09.9541441
TAGGAGT3450.09.9116494
GAACAAA21800.09.7196111
TACACCG800.00452618079.4980425
GTATAGG3600.09.2377471
GTTCTAG3100.09.1951771
GTCTAGG3750.09.1216151
GGTCGTC1054.513287E-49.0441486
GGGTAAG36350.09.03891857
TGGGTAA35900.09.0202166
Produced by FastQC (version 0.11.5)