FastQCFastQC Report
Wed 25 May 2016
SRR1294692_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294692_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5351431
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC2597944.854664107600378No Hit
GTACATGGGAAGCAGTGGTATCAAC2216314.14152775210967No Hit
GAGTACATGGGAAGCAGTGGTATCA1066771.9934294210277588No Hit
CATGTACTCTGCGTTGATACCACTG933011.7434775857149236No Hit
GCGTTGATACCACTGCTTCCCATGT833571.557658129199461No Hit
ACGCAGAGTACATGGGAAGCAGTGG688011.2856561170273895No Hit
GTATCAACGCAGAGTACATGGGAAG627201.1720229598400875No Hit
ACTCTGCGTTGATACCACTGCTTCC592521.1072178637826031No Hit
GCTTCCCATGTACTCTGCGTTGATA557951.0426183202212642No Hit
TATCAACGCAGAGTACATGGGAAGC549291.0264357327974518No Hit
GGTATCAACGCAGAGTACATGGGAA413180.7720925487033282No Hit
CAGTGGTATCAACGCAGAGTACATG361680.6758566073261526No Hit
GTGGTATCAACGCAGAGTACATGGG312750.5844231197225565No Hit
ATACCACTGCTTCCCATGTACTCTG310760.5807044882013801No Hit
GCAGTGGTATCAACGCAGAGTACAT282610.5281017357787104No Hit
CATGGGAAGCAGTGGTATCAACGCA272580.5093590854483595No Hit
GTACTCTGCGTTGATACCACTGCTT266560.4981097579320372No Hit
GGGAAGCAGTGGTATCAACGCAGAG258910.483814516154651No Hit
CTGCTTCCCATGTACTCTGCGTTGA254110.474844952686487No Hit
ACATGGGAAGCAGTGGTATCAACGC243790.4555603912299345No Hit
TACCACTGCTTCCCATGTACTCTGC242430.4530190149139548No Hit
GATACCACTGCTTCCCATGTACTCT229790.42939916444778975No Hit
CCATGTACTCTGCGTTGATACCACT228160.42635325018672576No Hit
CTTCCCATGTACTCTGCGTTGATAC228010.4260729513283456No Hit
GTACATGGTAAGCAGTGGTATCAAC201070.3757312763632756No Hit
GCAGAGTACATGGGAAGCAGTGGTA198770.3714333605347803No Hit
CCACTGCTTCCCATGTACTCTGCGT197780.3695833880694715No Hit
AAGCAGTGGTATCAACGCAGAGTAC197500.36906016353382864No Hit
ACCATGTACTCTGCGTTGATACCAC188520.3522796052121386No Hit
GTTGATACCACTGCTTCCCATGTAC185490.34661756827286005No Hit
ATGGGAAGCAGTGGTATCAACGCAG167220.3124771673221611No Hit
GTACATGGGTAAGCAGTGGTATCAA148060.2766736598117401No Hit
TGATACCACTGCTTCCCATGTACTC143070.2673490511229613No Hit
TTCCCATGTACTCTGCGTTGATACC142780.26680713999675976No Hit
ATCAACGCAGAGTACATGGGAAGCA132790.24813923602864355No Hit
GGTAAGCAGTGGTATCAACGCAGAG127890.23898280665489288No Hit
CCCCATGTACTCTGCGTTGATACCA116480.21766140682744484No Hit
GTATCAACGCAGAGTACTTTTTTTT108360.20248789529380085No Hit
AAAAAGTACTCTGCGTTGATACCAC85480.15973297609555276No Hit
TATCAACGCAGAGTACTTTTTTTTT85090.15900419906376442No Hit
GCTTACCATGTACTCTGCGTTGATA80550.15052048695012604No Hit
GGAAGCAGTGGTATCAACGCAGAGT80100.14967959037498568No Hit
ATTCCATTCCATTCCATTCCATTCC79340.1482594094925264No Hit
TCCATGTACTCTGCGTTGATACCAC76640.14321403004168418No Hit
GAGTACATGGTAAGCAGTGGTATCA74390.13900954716598232No Hit
GGTATCAACGCAGAGTACTTTTTTT72370.13523485587313No Hit
GTACTTTTTTTTTTTTTTTTTTTTT71730.1340389140773748No Hit
ACTCTGCGTTGATACCACTGCTTAC70350.13146016458027768No Hit
GAATGGAATGGAATGGAATGGAATG70190.13116117913133887No Hit
CTGCGTTGATACCACTGCTTCCCAT69290.12947938598105815No Hit
CTCTGCGTTGATACCACTGCTTCCC67290.12574206786932318No Hit
TGGGAAGCAGTGGTATCAACGCAGA61130.11423112808517945No Hit
ACGCAGAGTACTTTTTTTTTTTTTT60400.1128670069743962No Hit
TCCCATGTACTCTGCGTTGATACCA59670.11150288586361293No Hit
GCGTTGATACCACTGCTTACCATGT58880.11002664520947762No Hit
CTGCTTACCCATGTACTCTGCGTTG55510.10372926419120418No Hit
AAAGTACTCTGCGTTGATACCACTG54710.1022343369465102No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACGA400.00530556914.24016912
GGCGAAC550.003085823712.08268110
CGAATTT907.5072603E-611.6059219
TACGACC600.005803097511.1026584
TATCGCG600.00590788111.0759977
GATAGCG600.00590788111.0759977
GTGCGAC700.001500933810.84975411
TCGCGCG700.001500933810.8497549
AAAAAGT15450.010.8454471
CTAGGAC3600.010.8383093
CGCGCGC909.590087E-510.54837210
AAAAGTA16150.010.3124532
GTCCTAG3050.010.3009521
TGGACGG1951.4551915E-1110.2486095
GTACTAG1409.4034476E-810.2006191
GCGTTAT951.6099885E-410.021661
TACGGGG951.6162415E-410.0178122
TATACTG6250.09.7449635
TATTCCG2650.09.6961225
GCGTAGA1701.6101694E-89.5205771
Produced by FastQC (version 0.11.5)