FastQCFastQC Report
Wed 25 May 2016
SRR1294691_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294691_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6233059
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT77210.12387176184278055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25650.012.1129931
CGTCGTA700.001498662910.8517759
ATACCGT1304.2672236E-69.4959896
TCGGACG800.0045371389.4953039
GTCCTAG6250.09.4254651
GTATCAA50150.09.3783271
TATCGCG2357.2759576E-129.2933497
CCTACAC8200.09.266983
GTCCTAA5200.09.1360361
CGTATAT2201.6916601E-109.0668993
GTCGTAT2002.6539055E-99.0264031
CGACCGG850.00746876148.93697112
GGACTGT13300.08.9248026
TCGCGCG2356.730261E-118.889229
CGCGCGC3000.08.86242510
CTATACT8550.08.7758314
TGCGCTG6100.08.7194475
CCGTATA2304.0563464E-108.67289452
AGGACTG11400.08.6648155
GTTCTAG6400.08.6107141
Produced by FastQC (version 0.11.5)