##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294691_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6233059 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.966953144515397 33.0 33.0 33.0 27.0 33.0 2 30.972606708840715 33.0 33.0 33.0 27.0 33.0 3 30.931870370551604 33.0 33.0 33.0 27.0 33.0 4 34.39354448594182 37.0 37.0 37.0 27.0 37.0 5 34.58706102412957 37.0 37.0 37.0 27.0 37.0 6 34.691203628908376 37.0 37.0 37.0 27.0 37.0 7 34.676972574782305 37.0 37.0 37.0 27.0 37.0 8 34.60969132491767 37.0 37.0 37.0 27.0 37.0 9 34.36182779595059 37.0 37.0 37.0 27.0 37.0 10 34.438417797745856 37.0 37.0 37.0 27.0 37.0 11 34.66814223962905 37.0 37.0 37.0 27.0 37.0 12 34.58609665013599 37.0 37.0 37.0 27.0 37.0 13 34.51705061671966 37.0 37.0 37.0 27.0 37.0 14 35.59774197548908 37.0 37.0 40.0 27.0 40.0 15 35.91025931248204 37.0 37.0 40.0 27.0 40.0 16 35.92618054794604 37.0 37.0 40.0 27.0 40.0 17 35.86185498966078 37.0 37.0 40.0 27.0 40.0 18 35.8580278158766 37.0 37.0 40.0 27.0 40.0 19 35.93109178013557 40.0 37.0 40.0 27.0 40.0 20 35.823714166671614 37.0 37.0 40.0 27.0 40.0 21 35.76093487964738 37.0 37.0 40.0 27.0 40.0 22 35.61311773240074 37.0 37.0 40.0 27.0 40.0 23 35.19809807672284 37.0 33.0 40.0 27.0 40.0 24 35.517006497130865 37.0 33.0 40.0 27.0 40.0 25 34.2777039331731 37.0 33.0 40.0 22.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.43076630965909146 1101 2 0.5586874417278089 1101 3 0.4243214810631777 1101 4 0.466992434615328 1101 5 0.5109719586748014 1101 6 0.36914410511292317 1101 7 0.31877434985641884 1101 8 0.4238494802078989 1101 9 0.7117442960570841 1101 10 0.6284976791891594 1101 11 0.5184713028996342 1101 12 0.5315340859322149 1101 13 0.5397951265338605 1101 14 1.1172484154680973 1101 15 0.9318875009206806 1101 16 0.7853047242884656 1101 17 0.8588186163891791 1101 18 0.7874362110145512 1101 19 0.7778099132936305 1101 20 0.888210178994612 1101 21 0.8430608554240493 1101 22 1.0100149204465012 1101 23 1.2422777155999185 1101 24 1.0807184675247967 1101 25 0.8968138244944441 1102 1 0.25882938679217204 1102 2 0.43732116173092095 1102 3 0.28764117698330693 1102 4 0.22804745343755428 1102 5 0.22982766224295403 1102 6 0.18300845362542617 1102 7 0.22138629670926946 1102 8 0.3083532641312914 1102 9 0.5115230131549922 1102 10 0.5882947136185024 1102 11 0.3740643252779634 1102 12 0.37772949101599096 1102 13 0.3882971503605219 1102 14 1.0350905816196203 1102 15 0.7658773132421217 1102 16 0.4510752734620027 1102 17 0.6944576607437938 1102 18 0.7562099988406601 1102 19 0.6777617451690716 1102 20 0.80583770578491 1102 21 0.8094327382712407 1102 22 0.8246134930763489 1102 23 1.1336281001647635 1102 24 0.8930314707398992 1102 25 0.6697375241636152 1103 1 0.3152929741828885 1103 2 0.44250531664422965 1103 3 0.2013543191265903 1103 4 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0.0010718585139528614 2213 17 0.15956634939993108 2213 18 0.160390745820564 2213 19 0.13881937235398567 2213 20 0.20208945753265795 2213 21 -0.44056961029897934 2213 22 -0.8595579243670315 2213 23 -0.08891606660251483 2213 24 0.0841718894683865 2213 25 -0.3984845370863397 2214 1 -0.16222238430762204 2214 2 -0.4008785311534169 2214 3 -0.37805821838718856 2214 4 -0.5033449591603869 2214 5 -0.20069980410484334 2214 6 -0.14291877757212745 2214 7 -0.40072060927388264 2214 8 -7.897609340759004E-4 2214 9 0.02509829602804814 2214 10 -1.981122396676227 2214 11 -0.8453986841560237 2214 12 -0.4462420874248707 2214 13 -0.20988049818487298 2214 14 -0.701753654143559 2214 15 -0.4260891985076114 2214 16 -0.5751817545028715 2214 17 -0.462955329199211 2214 18 -0.4027198817297091 2214 19 -1.2744430914223202 2214 20 -2.287409141103204 2214 21 -1.762577849572061 2214 22 -1.9507196899165393 2214 23 -2.4507031786975944 2214 24 -1.5178431142456006 2214 25 -1.79530405415602 2215 1 -0.21024559590410163 2215 2 -0.3393586117595113 2215 3 -0.7926119824722413 2215 4 -0.23870258678905998 2215 5 -0.11830744411994942 2215 6 -0.06663047608649464 2215 7 -0.09974631801090794 2215 8 -0.14799149338841744 2215 9 -0.1163083617591667 2215 10 -0.15954761929218364 2215 11 -0.48256891570777327 2215 12 -0.3399791715482223 2215 13 -0.2169654677264603 2215 14 -0.09955828215814932 2215 15 -0.4171725955711736 2215 16 -0.33040824082822695 2215 17 -0.17910362294447424 2215 18 -0.22649145190315778 2215 19 -2.253881339993036 2215 20 -2.322830195136774 2215 21 -3.254402330887636 2215 22 -2.237522856573122 2215 23 -3.434541505866676 2215 24 -2.3872995517521147 2215 25 -1.6780883900710037 2216 1 -1.5171668679497579 2216 2 -1.1181368106296468 2216 3 -0.8694000508206869 2216 4 -0.7816627450825848 2216 5 -1.7581480468365172 2216 6 -1.3849401022675991 2216 7 -1.0433179827014811 2216 8 -5.145390502222806 2216 9 -4.095976734626959 2216 10 -1.9813709532047952 2216 11 -0.9421008429321702 2216 12 -1.1307138924770044 2216 13 -1.8523937465111473 2216 14 -3.4110247174233095 2216 15 -1.8890286974247061 2216 16 -2.916028813229296 2216 17 -2.5804690389997447 2216 18 -2.662990854003091 2216 19 -1.538954043497462 2216 20 -0.89882029374062 2216 21 -0.644428075575874 2216 22 -0.6186802678670063 2216 23 -0.8059056490960828 2216 24 -0.4006734023483034 2216 25 -0.10932931355334574 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 8.0 7 143.0 8 800.0 9 2173.0 10 4176.0 11 7740.0 12 12699.0 13 18040.0 14 23034.0 15 28288.0 16 31263.0 17 33769.0 18 35571.0 19 37173.0 20 38918.0 21 42185.0 22 44922.0 23 48232.0 24 52063.0 25 56394.0 26 61450.0 27 68887.0 28 78408.0 29 92953.0 30 111165.0 31 134852.0 32 179507.0 33 250190.0 34 357921.0 35 553067.0 36 938076.0 37 2888991.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.5048048591666 19.503908722440265 13.760898396326096 24.230388022067046 2 15.931611978157115 19.18776267043894 40.00969810710407 24.870927244299875 3 18.93228288688129 23.64669516475514 28.940285437684327 28.48073651067924 4 12.983037046914944 16.174926050754866 36.50086277462478 34.34117412770541 5 14.107140994544823 36.96918735592804 34.848175335923855 14.075496313603281 6 33.25860936731124 36.47710942114009 16.529757407563988 13.734523803984683 7 28.580057795863674 31.541470104470225 21.48302607488049 18.39544602478561 8 25.798214504881674 36.585370940661846 19.429889756225126 18.186524798231353 9 24.940390147328024 17.26633755974221 20.707071841463943 37.086200451465814 10 15.497766503830466 29.648424295445587 32.7293542987682 22.12445490195575 11 34.22112819025289 24.073765977235073 22.661253978616106 19.043851853895926 12 22.31728402647769 25.61570063697844 30.157158142095142 21.909857194448726 13 27.85009903071035 22.24965365322646 25.92790050883094 23.97234680723225 14 21.589488505098007 22.011562926759147 26.887189909745253 29.51175865839759 15 23.189134510251574 30.2023682224053 23.31114745918663 23.297349808156497 16 23.14071955336518 29.27625277518313 24.708042231236007 22.87498544021568 17 21.877870850052382 28.874089309808742 26.926459711434948 22.32158012870393 18 22.362258515607802 28.559157298647758 27.443049055111995 21.635535130632444 19 22.781720832575942 27.850835571272516 27.54863958058354 21.818804015568002 20 23.080182382914277 27.64294790343075 27.564960276205515 21.711909437449464 21 23.354788965913226 26.953622372089097 27.395960145445297 22.295628516552384 22 23.201872614015482 26.866789792847136 27.531963381976365 22.399374211161025 23 23.449552473461978 26.988420794722874 27.376990010330378 22.185036721484774 24 23.0282651968129 27.084240801501558 27.344292099423694 22.54320190226185 25 23.106927814005363 27.203201534390153 27.188199165849014 22.501671485755466 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2663.0 1 2663.0 2 2927.0 3 3191.0 4 3191.0 5 3191.0 6 4713.0 7 6235.0 8 6235.0 9 6235.0 10 10308.0 11 14381.0 12 14381.0 13 14381.0 14 25487.0 15 36593.0 16 36593.0 17 36593.0 18 62400.5 19 88208.0 20 88208.0 21 88208.0 22 134006.5 23 179805.0 24 179805.0 25 179805.0 26 242090.5 27 304376.0 28 304376.0 29 304376.0 30 370849.5 31 437323.0 32 437323.0 33 437323.0 34 496081.0 35 554839.0 36 554839.0 37 554839.0 38 599804.5 39 644770.0 40 644770.0 41 644770.0 42 670308.0 43 695846.0 44 695846.0 45 695846.0 46 703754.5 47 711663.0 48 711663.0 49 711663.0 50 699668.5 51 687674.0 52 687674.0 53 687674.0 54 653651.0 55 619628.0 56 619628.0 57 619628.0 58 556526.0 59 493424.0 60 493424.0 61 493424.0 62 428661.5 63 363899.0 64 363899.0 65 363899.0 66 289659.0 67 215419.0 68 215419.0 69 215419.0 70 161797.0 71 108175.0 72 108175.0 73 108175.0 74 76298.5 75 44422.0 76 44422.0 77 44422.0 78 29458.5 79 14495.0 80 14495.0 81 14495.0 82 9494.5 83 4494.0 84 4494.0 85 4494.0 86 2818.5 87 1143.0 88 1143.0 89 1143.0 90 721.0 91 299.0 92 299.0 93 299.0 94 177.0 95 55.0 96 55.0 97 55.0 98 47.0 99 39.0 100 39.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029953189918465396 2 0.014679790452809769 3 0.020792358936438753 4 0.022781751303814067 5 0.02188331604112844 6 0.005486872497115782 7 0.0013957833545294534 8 8.663482890182813E-4 9 4.6526111817648446E-4 10 9.626092100203128E-5 11 8.021743416835939E-4 12 6.096524996795314E-4 13 0.00231026210404875 14 5.615220391785157E-4 15 0.0018289574990385938 16 0.0021979576962130472 17 0.00239047953821711 18 0.0024225665118844534 19 2.8878276300609376E-4 20 3.048262498397657E-4 21 0.08042599949719713 22 0.07636699732827813 23 0.07740982397246682 24 9.305222363529689E-4 25 0.22526659863158685 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 6233059.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.81245889864814 #Duplication Level Percentage of deduplicated Percentage of total 1 69.46473517072917 33.90744530426695 2 14.89507292077136 14.541302694750376 3 6.209088062329603 9.092425675216518 4 3.176459413687293 6.202031782953397 5 1.7858129431709366 4.35849604446026 6 1.124943726714721 3.2946761654132635 7 0.7069068072154892 2.415410163066645 8 0.5218723355574112 2.0379097543790117 9 0.3677315680255417 1.6154893844983969 >10 1.5998824293465008 13.31257152936046 >50 0.09200969987613053 3.116335540005948 >100 0.052493456432627095 4.58420781644025 >500 0.0022602361223893995 0.70674274493602 >1k 6.647545645809942E-4 0.608275922653705 >5k 6.647545638883428E-5 0.20667947759868802 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7721 0.12387176184278055 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.6043486833671878E-5 0.0 0.0 0.0 6 0.0 1.6043486833671878E-5 0.0 0.0 0.0 7 0.0 1.6043486833671878E-5 0.0 1.6043486833671878E-5 0.0 8 0.0 1.6043486833671878E-5 0.0 1.6043486833671878E-5 0.0 9 0.0 1.6043486833671878E-5 0.0 3.2086973667343756E-5 0.0 10 0.0 1.6043486833671878E-5 0.0 3.2086973667343756E-5 0.0 11 0.0 1.6043486833671878E-5 0.0 3.2086973667343756E-5 0.0 12 0.0 1.6043486833671878E-5 0.0 3.2086973667343756E-5 0.0 13 0.0 1.6043486833671878E-5 0.0 3.2086973667343756E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2565 0.0 12.112993 1 CGTCGTA 70 0.0014986629 10.851775 9 ATACCGT 130 4.2672236E-6 9.495989 6 TCGGACG 80 0.004537138 9.495303 9 GTCCTAG 625 0.0 9.425465 1 GTATCAA 5015 0.0 9.378327 1 TATCGCG 235 7.2759576E-12 9.293349 7 CCTACAC 820 0.0 9.26698 3 GTCCTAA 520 0.0 9.136036 1 CGTATAT 220 1.6916601E-10 9.066899 3 GTCGTAT 200 2.6539055E-9 9.026403 1 CGACCGG 85 0.0074687614 8.936971 12 GGACTGT 1330 0.0 8.924802 6 TCGCGCG 235 6.730261E-11 8.88922 9 CGCGCGC 300 0.0 8.862425 10 CTATACT 855 0.0 8.775831 4 TGCGCTG 610 0.0 8.719447 5 CCGTATA 230 4.0563464E-10 8.6728945 2 AGGACTG 1140 0.0 8.664815 5 GTTCTAG 640 0.0 8.610714 1 >>END_MODULE