FastQCFastQC Report
Wed 25 May 2016
SRR1294691_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294691_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6233059
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT204840.32863478430093473No Hit
GGTATCAACGCAGAGTACTTTTTTT145910.23409051639010636No Hit
TATCAACGCAGAGTACTTTTTTTTT136370.2187850299507834No Hit
ACGCAGAGTACTTTTTTTTTTTTTT75980.12189841296223893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39200.013.3094121
ACCGCGT604.116056E-412.6590038
CGCCCTA4300.09.93576516
GTATTAA10250.09.7528111
GTCGTAC1002.6984757E-49.52061
CTAGTAC3800.09.5144793
GTATCAA76700.09.4833621
TCGCGCG2950.09.3332589
TACGCTG2054.129106E-109.2824185
GTCTTAG4850.09.22615051
TATCGCG3000.09.1777777
ATACCGT1356.8991067E-69.143646
TACGACC1252.7081236E-59.1338994
CTGGACG4900.09.1261324
GCGCGCC3900.09.00715111
TGGACGG3600.08.9858965
GGACCGG3400.08.936776
CGCGCCT3300.08.91862912
TGTATCG3850.08.8966555
GCTCGTT2900.08.83926411
Produced by FastQC (version 0.11.5)