FastQCFastQC Report
Wed 25 May 2016
SRR1294690_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294690_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4484157
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1192242.658782910589437No Hit
CCCATGTACTCTGCGTTGATACCAC1164692.5973443837938768No Hit
GAGTACATGGGAAGCAGTGGTATCA530381.1827864189411743No Hit
CATGTACTCTGCGTTGATACCACTG418770.9338879080281979No Hit
GCGTTGATACCACTGCTTCCCATGT381270.8502601492320631No Hit
GTATCAACGCAGAGTACATGGGAAG336530.7504866578043543No Hit
ACGCAGAGTACATGGGAAGCAGTGG312730.6974109068884073No Hit
GCTTCCCATGTACTCTGCGTTGATA312180.696184366426064No Hit
ACTCTGCGTTGATACCACTGCTTCC250600.5588564361149709No Hit
TATCAACGCAGAGTACATGGGAAGC243910.5439372439457405No Hit
GGTATCAACGCAGAGTACATGGGAA207450.4626287616602184No Hit
CAGTGGTATCAACGCAGAGTACATG174010.38805510154974504No Hit
GTGGTATCAACGCAGAGTACATGGG172880.38553511841802146No Hit
ATACCACTGCTTCCCATGTACTCTG136680.3048064552601526No Hit
GCAGTGGTATCAACGCAGAGTACAT135800.3028439905204033No Hit
GTACATGGTAAGCAGTGGTATCAAC127300.28388836519327937No Hit
CATGGGAAGCAGTGGTATCAACGCA125220.27924981217205375No Hit
GTACTCTGCGTTGATACCACTGCTT124580.2778225650885997No Hit
ACATGGGAAGCAGTGGTATCAACGC120300.2682778502180008No Hit
GGGAAGCAGTGGTATCAACGCAGAG117700.2624796589414688No Hit
CTGCTTCCCATGTACTCTGCGTTGA114360.2550312132246931No Hit
GATACCACTGCTTCCCATGTACTCT105170.2345368371357203No Hit
CTTCCCATGTACTCTGCGTTGATAC101760.22693228626919174No Hit
CCATGTACTCTGCGTTGATACCACT99310.22146860602784424No Hit
TACCACTGCTTCCCATGTACTCTGC99020.22082188469315414No Hit
GCAGAGTACATGGGAAGCAGTGGTA90060.20084042552479764No Hit
ACCATGTACTCTGCGTTGATACCAC89270.19907866740615904No Hit
GTACATGGGTAAGCAGTGGTATCAA87580.19530984307641325No Hit
AAGCAGTGGTATCAACGCAGAGTAC87120.19428400923518066No Hit
CCACTGCTTCCCATGTACTCTGCGT85640.1909835003546932No Hit
GTTGATACCACTGCTTCCCATGTAC83870.18703627013951563No Hit
ATGGGAAGCAGTGGTATCAACGCAG79600.17751385600459574No Hit
TTCCCATGTACTCTGCGTTGATACC74550.16625198448671621No Hit
TGATACCACTGCTTCCCATGTACTC67830.15126589011044886No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA66580.1484782981505777No Hit
GGTAAGCAGTGGTATCAACGCAGAG63880.142457099517256No Hit
CCCCATGTACTCTGCGTTGATACCA63150.1408291458126912No Hit
ATCAACGCAGAGTACATGGGAAGCA62090.13846526783072047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAAA2900.011.1373822
GTACAAA11400.010.5851731
GTCTAGG3250.09.6478311
ACGCAAA3950.09.6219251
GTCCTAC2500.09.5016521
GTCTTGG5650.09.4175661
TGGACTG5200.09.3156015
GGACTGT5050.09.2141816
CTGGTCG1356.8938407E-69.1440259
GTATTAG5000.09.1215861
GTACAAG5650.09.0812251
GTACTAG2106.675691E-109.0491911
CTAGACC2950.09.0153984
GACCGTC950.00183235548.9959437
ACGGGTC850.0074505068.9395385
AGACCGT850.00746346648.9376446
ATCGTGT850.007466888.9371468
ACTGTCC4800.08.9022368
TTGGACT3100.08.8855684
GTCTAGA4000.08.7890271
Produced by FastQC (version 0.11.5)