FastQCFastQC Report
Wed 25 May 2016
SRR1294690_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294690_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4484157
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1308362.917739053293629No Hit
GTACATGGGAAGCAGTGGTATCAAC1138942.539919989420531No Hit
GAGTACATGGGAAGCAGTGGTATCA583111.3003781981763796No Hit
CATGTACTCTGCGTTGATACCACTG491461.0959919556786257No Hit
GCGTTGATACCACTGCTTCCCATGT425040.9478704692989117No Hit
ACGCAGAGTACATGGGAAGCAGTGG377450.8417412682026967No Hit
GCTTCCCATGTACTCTGCGTTGATA353720.7888216224365024No Hit
GTATCAACGCAGAGTACATGGGAAG341150.7607895976880381No Hit
ACTCTGCGTTGATACCACTGCTTCC299070.6669481019509353No Hit
TATCAACGCAGAGTACATGGGAAGC281990.6288584454112557No Hit
GGTATCAACGCAGAGTACATGGGAA220360.49141901142176775No Hit
CAGTGGTATCAACGCAGAGTACATG205390.45803481011035074No Hit
GTGGTATCAACGCAGAGTACATGGG184220.4108241526779727No Hit
ATACCACTGCTTCCCATGTACTCTG158330.35308754800512115No Hit
CATGGGAAGCAGTGGTATCAACGCA156640.3493187236753753No Hit
GCAGTGGTATCAACGCAGAGTACAT153720.3428069088571163No Hit
ACATGGGAAGCAGTGGTATCAACGC148900.33205795425985307No Hit
GGGAAGCAGTGGTATCAACGCAGAG135610.30242027654250286No Hit
CTGCTTCCCATGTACTCTGCGTTGA132890.2963544764378232No Hit
GTACTCTGCGTTGATACCACTGCTT126130.28127917911883993No Hit
CCATGTACTCTGCGTTGATACCACT122200.272514989997005No Hit
CTTCCCATGTACTCTGCGTTGATAC121950.27195747160503075No Hit
GATACCACTGCTTCCCATGTACTCT120060.26774263256170555No Hit
GTACATGGTAAGCAGTGGTATCAAC118790.26491043913047646No Hit
TACCACTGCTTCCCATGTACTCTGC116230.25920145079666035No Hit
GTATCAACGCAGAGTACTTTTTTTT109080.24325642478619727No Hit
AAGCAGTGGTATCAACGCAGAGTAC108450.24185147843842222No Hit
ACCATGTACTCTGCGTTGATACCAC103140.2300097877928895No Hit
GCAGAGTACATGGGAAGCAGTGGTA99690.22231603398364508No Hit
CCACTGCTTCCCATGTACTCTGCGT99380.22162471117759702No Hit
ATGGGAAGCAGTGGTATCAACGCAG96430.2150459941523011No Hit
GTACATGGGTAAGCAGTGGTATCAA92960.20730763887169876No Hit
GTTGATACCACTGCTTCCCATGTAC91390.20380642337010058No Hit
TATCAACGCAGAGTACTTTTTTTTT80500.17952092221570298No Hit
GGTATCAACGCAGAGTACTTTTTTT77850.1736112272607761No Hit
TTCCCATGTACTCTGCGTTGATACC77240.17225088238435896No Hit
GGTAAGCAGTGGTATCAACGCAGAG74980.1672109161209119No Hit
TGATACCACTGCTTCCCATGTACTC74090.16522615064548365No Hit
CCCCATGTACTCTGCGTTGATACCA73200.16324138517005538No Hit
ATCAACGCAGAGTACATGGGAAGCA69780.15561453356784785No Hit
ACGCAGAGTACTTTTTTTTTTTTTT56060.12501792421630198No Hit
GCTTACCATGTACTCTGCGTTGATA51640.11516099904619753No Hit
AAAAAGTACTCTGCGTTGATACCAC50450.11250721150040019No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA48220.10753414744399004No Hit
GTACTTTTTTTTTTTTTTTTTTTTT47320.10552708123288279No Hit
GAGTACATGGTAAGCAGTGGTATCA44940.10021950614128808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGT957.4471245E-812.9935236
GTGTACG1207.185008E-1012.693481
CGCAAAA1156.9321686E-811.5834822
CTAGGAC3600.011.3650163
GCGTAGA951.6106546E-410.0211681
ATAGACG951.6195823E-410.015683
TGTGCGG951.6557366E-49.99379110
AGTACTC13900.09.92561155
CTACGCA1106.726128E-59.5148964
GTCCTAT2650.09.3404851
GTCTAAG3100.09.213011
CTGTGCG1356.9077687E-69.142579
TAGACAG6350.09.1402945
AAAAGTA12350.09.0912572
AAAAAGT12400.09.059461
TAGGACT3700.09.0005774
TACGAGA850.00734431958.9551964
GTGTAGG4700.08.9124431
TACCGTG1502.725912E-68.8615567
TAGACTG5300.08.7967915
Produced by FastQC (version 0.11.5)