FastQCFastQC Report
Wed 25 May 2016
SRR1294689_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294689_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4024013
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC254360.6321053137750798No Hit
CCCATGTACTCTGCGTTGATACCAC233990.581484204946654No Hit
GAGTACATGGGAAGCAGTGGTATCA116660.2899096001926435No Hit
GCGTTGATACCACTGCTTCCCATGT82140.20412458906072123No Hit
CATGTACTCTGCGTTGATACCACTG81960.20367727440244354No Hit
GTATCAACGCAGAGTACATGGGAAG76370.18978566918148623No Hit
ACGCAGAGTACATGGGAAGCAGTGG68950.17134636493470573No Hit
GTATCAACGCAGAGTACTTTTTTTT59000.14661980465768873No Hit
ATTCCATTCCATTCCATTCCATTCC58030.14420927566585895No Hit
TATCAACGCAGAGTACATGGGAAGC57300.1423951662183994No Hit
ACTCTGCGTTGATACCACTGCTTCC55600.13817052777911007No Hit
GGTATCAACGCAGAGTACATGGGAA54280.13489022028507364No Hit
GTGGTATCAACGCAGAGTACATGGG54020.13424409911200585No Hit
GAATGGAATGGAATGGAATGGAATG51030.12681370562172636No Hit
GCTTCCCATGTACTCTGCGTTGATA47720.11858808607228655No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATA500.001501098713.2990262
GACCGCG1001.941713E-611.3951987
CTAGACG856.609034E-410.0569614
TAGGACC2550.010.0569614
CTGATCG856.623396E-410.0545879
ATTAGAC3350.09.6406053
CTAGGAC3950.09.3785933
GTCCTAG3550.09.367841
TCTGTCG1951.6989361E-99.2525118
GTCTAGG3800.09.2516221
AGGACCG1451.7130133E-69.1704875
TACACTG9100.09.080515
TAGGACG950.00182784538.9983344
GTCCGGA850.0074283498.9427441
CGCGCGA850.007464038.93752212
CTACACT6400.08.9046014
GCTACAC3900.08.7681523
ACGGTAC1856.596383E-88.7286573
GGACCGT1201.773104E-48.7046666
TTAAGAC3500.08.6846463
Produced by FastQC (version 0.11.5)