##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294689_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4024013
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.090544190587853	33.0	33.0	33.0	27.0	33.0
2	31.072028842849164	33.0	33.0	33.0	27.0	33.0
3	31.066921006467922	33.0	33.0	33.0	27.0	33.0
4	34.435797299859615	37.0	37.0	37.0	27.0	37.0
5	34.67763175715387	37.0	37.0	37.0	27.0	37.0
6	34.847049698895106	37.0	37.0	37.0	33.0	37.0
7	34.888869643313775	37.0	37.0	37.0	33.0	37.0
8	34.849332991717475	37.0	37.0	37.0	33.0	37.0
9	34.52823164338684	37.0	37.0	37.0	27.0	37.0
10	34.63861995475661	37.0	37.0	37.0	27.0	37.0
11	34.85143487359509	37.0	37.0	37.0	33.0	37.0
12	34.79368257508114	37.0	37.0	37.0	27.0	37.0
13	34.69883969062724	37.0	37.0	37.0	27.0	37.0
14	35.860358552519585	40.0	37.0	40.0	27.0	40.0
15	36.15887771734336	40.0	37.0	40.0	27.0	40.0
16	36.172133887241415	40.0	37.0	40.0	27.0	40.0
17	36.12040443209304	40.0	37.0	40.0	27.0	40.0
18	36.12737931015631	40.0	37.0	40.0	27.0	40.0
19	36.207166080228866	40.0	37.0	40.0	27.0	40.0
20	36.11179660701892	40.0	37.0	40.0	27.0	40.0
21	36.06479750438182	40.0	37.0	40.0	27.0	40.0
22	35.92552906762478	40.0	37.0	40.0	27.0	40.0
23	35.543189100035214	37.0	37.0	40.0	27.0	40.0
24	35.87046711827223	37.0	37.0	40.0	27.0	40.0
25	34.730719806322696	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.3605532990576421
1101	2	0.4787425587714118
1101	3	0.3756198900978305
1101	4	0.37056353608295467
1101	5	0.3047160524958201
1101	6	0.22867686977161839
1101	7	0.34351966873597206
1101	8	0.4646175707501996
1101	9	0.63638779512263
1101	10	0.6608626509125628
1101	11	0.40206525404151705
1101	12	0.45438396146892046
1101	13	0.4103193406772476
1101	14	1.127731808893536
1101	15	0.8352772192372626
1101	16	0.7143674056985176
1101	17	0.7544322424349446
1101	18	0.7949697253435559
1101	19	0.679730758005924
1101	20	0.8716324183769046
1101	21	0.8604654406610024
1101	22	0.961047781713745
1101	23	1.232319183924858
1101	24	1.0662977129975246
1101	25	0.7504185562263572
1102	1	0.2695862266099418
1102	2	0.517994422949549
1102	3	0.31912445464630323
1102	4	0.21759055368880809
1102	5	0.12063430140386089
1102	6	0.23028207315460492
1102	7	0.3133648411498271
1102	8	0.3999009291498723
1102	9	0.6462297102050272
1102	10	0.5999188128912394
1102	11	0.49201808277565817
1102	12	0.48068598033241017
1102	13	0.50623256718589
1102	14	1.1807799407457438
1102	15	0.849084737179254
1102	16	0.6262781867603167
1102	17	0.7470567882928165
1102	18	0.713660191825717
1102	19	0.6803621720066317
1102	20	0.7837426439552715
1102	21	0.8306570397187087
1102	22	0.8973132651864333
1102	23	1.202679711876037
1102	24	0.9695800048951142
1102	25	0.8895314999976875
1103	1	0.32413746465832105
1103	2	0.48142477928866256
1103	3	0.28653086861116606
1103	4	-0.009906683594628873
1103	5	0.17918638202544201
1103	6	0.08648532858556734
1103	7	0.20013299728832834
1103	8	0.4473721830675146
1103	9	0.6298077273252716
1103	10	0.5459233959788108
1103	11	0.3519444571053114
1103	12	0.40802859059682106
1103	13	0.40985696033565233
1103	14	0.9942686682238602
1103	15	0.749863116792298
1103	16	0.45469498584818524
1103	17	0.4894892278901466
1103	18	0.6001742591235768
1103	19	0.6566448462300016
1103	20	0.8257284887024738
1103	21	0.8012049313966401
1103	22	0.7726699082165425
1103	23	1.2501256345422789
1103	24	0.8279376818198187
1103	25	0.6270923747901733
1104	1	0.3080405195143072
1104	2	0.5008795628185538
1104	3	0.36645098212861527
1104	4	0.2094261061317937
1104	5	0.17755491455280037
1104	6	0.15896996139867525
1104	7	0.24966005196750274
1104	8	0.38106082925855134
1104	9	0.5572717201163258
1104	10	0.573840529500103
1104	11	0.38563371630226584
1104	12	0.3506606277150581
1104	13	0.3853018550113916
1104	14	0.8292692933211896
1104	15	0.7148914098277999
1104	16	0.579223147031179
1104	17	0.7431422230061742
1104	18	0.6789732185431987
1104	19	0.6945671081926292
1104	20	0.7684417069267937
1104	21	0.769161818071133
1104	22	0.7683188315109959
1104	23	1.2280605143039836
1104	24	0.8863762398701596
1104	25	0.63917910826774
1105	1	0.33499017783661245
1105	2	0.36120955467505667
1105	3	0.3829317545469344
1105	4	0.34860359614921776
1105	5	0.3290178616344761
1105	6	0.33182694349240194
1105	7	0.3212269353691184
1105	8	0.4140123679584633
1105	9	-1.5445458766036566
1105	10	-0.025512397752237348
1105	11	0.1569880316635448
1105	12	0.3052600413166431
1105	13	0.32257543498536734
1105	14	-1.469280371209912
1105	15	-0.12132711865476153
1105	16	-0.003105100819439599
1105	17	0.2079735073451019
1105	18	0.17429674937346817
1105	19	0.39910235501713487
1105	20	0.46998916567295623
1105	21	0.6925165015251977
1105	22	0.6656522376498302
1105	23	1.0820578411964306
1105	24	0.820826121320998
1105	25	0.31889506148669255
1106	1	0.08628409713788443
1106	2	0.018569314807834303
1106	3	0.13786251340355093
1106	4	-1.1254290377014726
1106	5	-0.2897707988634224
1106	6	-0.08595285746196168
1106	7	-0.0211491796003358
1106	8	0.18360338275001453
1106	9	0.3516507527445043
1106	10	0.38902551051238277
1106	11	0.2626463814390618
1106	12	0.07770028776259608
1106	13	0.05593291820820667
1106	14	0.6388455044442694
1106	15	0.37998068288388254
1106	16	0.16522095781266444
1106	17	-0.032514065822375926
1106	18	0.20102315054559483
1106	19	0.19634608975155032
1106	20	0.32492499795768737
1106	21	0.5121510307194299
1106	22	0.5310729502824998
1106	23	0.7693632877723928
1106	24	0.39371216041761414
1106	25	0.3886320906544469
1107	1	0.42059911720752297
1107	2	0.30735312838360684
1107	3	0.25515463477255196
1107	4	0.47792830700608135
1107	5	0.476603844582975
1107	6	0.2768188467606407
1107	7	0.2880247421122064
1107	8	0.3481563285730118
1107	9	0.5936865880993949
1107	10	0.5848200650426065
1107	11	0.32213737540386234
1107	12	0.3977479455692503
1107	13	0.349326398466836
1107	14	0.784646724646052
1107	15	0.5483938352659194
1107	16	0.618794995841256
1107	17	0.5472955269680781
1107	18	0.5966819919365491
1107	19	0.5092846285373014
1107	20	0.6135667235460005
1107	21	0.61473546663062
1107	22	0.6140523681977044
1107	23	0.8446084992327272
1107	24	0.8387647414484007
1107	25	0.632729747346005
1108	1	0.5152008381927935
1108	2	0.4546803612232537
1108	3	0.6056815535762823
1108	4	0.7996953570622267
1108	5	0.6133778759140398
1108	6	0.5302563492974954
1108	7	0.4303346782465596
1108	8	0.5086012495626946
1108	9	0.29496421766985037
1108	10	0.5797421501781486
1108	11	0.4467443642764195
1108	12	0.4921295731141697
1108	13	0.43553921189821665
1108	14	-0.8883529724825578
1108	15	0.03927708666600438
1108	16	0.4404480655789982
1108	17	0.5101394163081565
1108	18	0.714822396067845
1108	19	0.5217227915112019
1108	20	0.699911868863623
1108	21	0.8290460787796832
1108	22	0.945701306560288
1108	23	1.1225283096828136
1108	24	0.9245244545132536
1108	25	0.85200669181215
1109	1	0.3791367271331616
1109	2	0.26084809768757466
1109	3	0.37655282369892973
1109	4	0.5273222772348092
1109	5	0.6143539719633608
1109	6	0.422150439267611
1109	7	0.2810343394251049
1109	8	0.34160543065924287
1109	9	0.5137303573055334
1109	10	0.5279604314552273
1109	11	0.33477777064233294
1109	12	0.350747876331134
1109	13	0.32705034929051635
1109	14	0.7892941816121279
1109	15	0.4633085952913589
1109	16	0.3961028389567076
1109	17	0.44160439333137447
1109	18	0.4329774117772942
1109	19	0.48717717402741556
1109	20	0.6253988735241762
1109	21	0.5810402356420639
1109	22	0.6544914792823988
1109	23	1.1297186499802763
1109	24	0.8160886498010456
1109	25	0.6727375762681049
1110	1	0.4224187901860077
1110	2	0.2840954188332745
1110	3	0.45172771054682315
1110	4	0.6985883787189948
1110	5	0.48652811102130045
1110	6	0.29931560714627636
1110	7	0.32087143573868815
1110	8	0.2604601849605501
1110	9	0.3276030291480154
1110	10	0.40209291581633977
1110	11	0.24339093806484158
1110	12	0.13123606683077327
1110	13	0.1498001849207924
1110	14	0.5771891849782165
1110	15	0.5166030295041821
1110	16	0.39103132919700556
1110	17	0.4492337151717223
1110	18	0.4994705989229544
1110	19	0.4642058463304153
1110	20	0.5713754863540856
1110	21	0.6510469581248302
1110	22	0.7024473445426835
1110	23	0.870731981027987
1110	24	0.6936406297979971
1110	25	0.6201547833785668
1111	1	0.1635033791508711
1111	2	0.003210960265786156
1111	3	0.298991162622368
1111	4	0.48938474658760356
1111	5	0.4789690931282493
1111	6	0.3129544469466268
1111	7	0.21103350391226172
1111	8	0.3561664866088279
1111	9	0.3373362165060456
1111	10	0.2790154198749093
1111	11	0.19935782000619184
1111	12	0.2283231713597189
1111	13	0.12703444782740547
1111	14	-1.8401596994148335
1111	15	-0.5962536734878014
1111	16	-0.01305008750345138
1111	17	0.1560745105320862
1111	18	0.19434947936731817
1111	19	0.20704892629619565
1111	20	0.30132308348251513
1111	21	0.38754600418305785
1111	22	0.2331806253447084
1111	23	-1.8550133938774351
1111	24	-0.3721932187734822
1111	25	0.13601069675115696
1112	1	-0.18031168640311535
1112	2	-0.3387863012385566
1112	3	-0.042105167764926676
1112	4	0.10995295062038934
1112	5	-0.8120453911038652
1112	6	-0.9373354617553105
1112	7	-1.1135835900111601
1112	8	-0.878385330525802
1112	9	-0.8561672429139335
1112	10	-1.1019109270509233
1112	11	-0.9661595866850732
1112	12	-1.0312979903721384
1112	13	-0.9641077181550983
1112	14	-0.8254847176076012
1112	15	-1.0317911365012975
1112	16	-0.9850557073039923
1112	17	-0.9572777790691873
1112	18	-1.1302125658337303
1112	19	-0.9302659364309704
1112	20	-1.1701378231140396
1112	21	-1.9194441805814364
1112	22	-1.76954179047204
1112	23	-1.5860404824956618
1112	24	-1.8507873862037627
1112	25	-2.1108942791242526
1113	1	0.11101435655623959
1113	2	-0.05496429176833573
1113	3	-0.10141241378622823
1113	4	0.2202013501772484
1113	5	0.14769542939054503
1113	6	0.025367408930740964
1113	7	0.01185375639687436
1113	8	0.0027538748611277697
1113	9	0.18369448346039263
1113	10	-0.08894243657063328
1113	11	-0.12503216557860952
1113	12	-0.028807557246565807
1113	13	-0.11029626099722378
1113	14	0.08470362236497664
1113	15	-0.07852497775514422
1113	16	0.012878629068104885
1113	17	0.0056505426942976555
1113	18	0.09617127445949336
1113	19	-0.08278413664299933
1113	20	-0.5646040462903983
1113	21	-0.4845353029717785
1113	22	-0.07768301462950689
1113	23	-0.24494474837423041
1113	24	0.016418013841231982
1113	25	-0.9473994068159755
1114	1	-0.8535307074775176
1114	2	-0.5907641991268449
1114	3	-0.37079767810224595
1114	4	-1.3905049134363026
1114	5	-0.4889016080473425
1114	6	-0.4526816634498019
1114	7	-0.3848436196491889
1114	8	-0.30080472230000765
1114	9	-0.2485349688453553
1114	10	-0.3921087039134292
1114	11	-0.3612178932575105
1114	12	-0.4391750618328132
1114	13	-0.40274073609969463
1114	14	-0.369080519300347
1114	15	-1.1556971101373037
1114	16	-0.6162039609642136
1114	17	-0.5950015864897935
1114	18	-0.5276315596131766
1114	19	-0.6370974858633431
1114	20	-1.8068750117307673
1114	21	-6.523214748916594
1114	22	-7.749241404437534
1114	23	-7.699522395096558
1114	24	-5.646063031931554
1114	25	-7.112122892348594
1115	1	-1.1485713656136873
1115	2	-1.0202062867484436
1115	3	-0.8502694020410253
1115	4	-0.6289030816073478
1115	5	-0.8019766386055238
1115	6	-0.9852331476799776
1115	7	-1.07374293293919
1115	8	-0.8875620541532214
1115	9	-2.802515896185465
1115	10	-1.6947963663731898
1115	11	-1.2282827191083925
1115	12	-1.068675901423461
1115	13	-1.4250431851595522
1115	14	-1.0627486275917946
1115	15	-2.071642803936797
1115	16	-1.350554598240727
1115	17	-3.0893674290954323
1115	18	-2.8838819648227414
1115	19	-2.0526860484298055
1115	20	-1.8122481588903483
1115	21	-1.3630050334872905
1115	22	-0.946966458393554
1115	23	-0.3075636452933779
1115	24	-1.4319400637655306
1115	25	-1.034787165761692
1116	1	-1.433668898576439
1116	2	-0.6875299619850743
1116	3	-0.5075685430868901
1116	4	-0.09094885642442563
1116	5	-0.14424902833485476
1116	6	-0.14311998165828044
1116	7	-0.4794937149621532
1116	8	-1.5140910034607273
1116	9	-0.5791152405620821
1116	10	-2.245950129536965
1116	11	-0.8245687378281943
1116	12	-0.580252475399682
1116	13	-0.5370648454833571
1116	14	-0.8265874862133273
1116	15	-0.9879267381417876
1116	16	-0.5535246799417521
1116	17	-0.40011978653652136
1116	18	-1.0329275683194012
1116	19	-0.6064370715071377
1116	20	-0.9147191395383913
1116	21	-0.28605530897781506
1116	22	0.05123898549696548
1116	23	0.2742101293212471
1116	24	0.10614276169803816
1116	25	-0.005836836184016647
1201	1	0.30822334641664995
1201	2	0.39394309036331876
1201	3	0.308603872044074
1201	4	0.32981581283590344
1201	5	0.3671556242715752
1201	6	0.23099483150892297
1201	7	0.3403598848094589
1201	8	0.4497508462456281
1201	9	0.6996237133322296
1201	10	0.6283530690378356
1201	11	0.48707529371130676
1201	12	0.543895580206204
1201	13	0.4565155049187837
1201	14	1.1174661778320925
1201	15	0.8594025124647615
1201	16	0.7248951298539268
1201	17	0.7204996339172638
1201	18	0.5479317678414048
1201	19	0.6514674603251578
1201	20	0.8233870969266661
1201	21	0.8225542531912495
1201	22	0.8071600819509328
1201	23	0.7329708295136683
1201	24	0.7574072606786544
1201	25	0.7555567958691185
1202	1	-0.27677229307382945
1202	2	-0.015198560774013714
1202	3	-0.026321042460544675
1202	4	-0.17547464090493747
1202	5	-0.07065760181954772
1202	6	-0.19182351594731273
1202	7	-0.13358389487904532
1202	8	0.09357572495544275
1202	9	0.1462350239117569
1202	10	0.36050720108893586
1202	11	0.12711382067182342
1202	12	0.11541272049586837
1202	13	0.24852908619112668
1202	14	0.4028736015923471
1202	15	0.41743634056557966
1202	16	0.35702032633624015
1202	17	0.21978754225030883
1202	18	0.10051737994684373
1202	19	0.19712392595494777
1202	20	0.34723089903734916
1202	21	0.30990736339023073
1202	22	0.25689879722320796
1202	23	0.6501259328084572
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2211	5	0.3536378453852649
2211	6	0.2787863670918469
2211	7	0.1633933379943926
2211	8	0.28085748163844926
2211	9	0.44816196795548535
2211	10	0.4900246941946875
2211	11	0.22867060860176736
2211	12	0.22501433897479473
2211	13	0.15221293276573533
2211	14	0.3519425554533768
2211	15	0.2655640443740168
2211	16	0.18816491680866676
2211	17	0.2024968886489873
2211	18	0.16577191575922967
2211	19	0.20921515707338756
2211	20	0.3298728122020478
2211	21	0.3705166285031538
2211	22	0.4769477699106588
2211	23	0.5713307832855534
2211	24	0.548169930996913
2211	25	0.5185321327004928
2212	1	-0.23363370859233967
2212	2	-0.36667204444906787
2212	3	-0.17920521347942042
2212	4	-0.4572778693517634
2212	5	-0.16604018360988704
2212	6	-0.4672981482388039
2212	7	-0.3879821772978147
2212	8	-0.28411793184228884
2212	9	-0.19116327153438561
2212	10	-0.6769078974542779
2212	11	-0.466346285883219
2212	12	-0.5861907718986856
2212	13	-0.7062879709175434
2212	14	-0.47635253740261163
2212	15	-0.6380124997447183
2212	16	-0.4447949270339322
2212	17	-0.5422868961321186
2212	18	-0.42140613526989057
2212	19	-0.45516512617594884
2212	20	-0.4935048315231114
2212	21	-0.44863969422407735
2212	22	-0.5137906508513623
2212	23	0.019245483642464478
2212	24	-0.2450663573127727
2212	25	-0.8916652073975442
2213	1	-0.1216379354306163
2213	2	-1.23929811638396
2213	3	-0.3440086039269872
2213	4	0.13376512159859288
2213	5	0.004241686810168233
2213	6	0.024663359067531587
2213	7	-0.02458615366388983
2213	8	0.14165028297070847
2213	9	0.02090144957696083
2213	10	-0.36115664293534877
2213	11	-0.08436898643697077
2213	12	0.014895083979482138
2213	13	-0.4743399328698601
2213	14	-0.015975597648093753
2213	15	-0.1416059644496812
2213	16	-0.13214628229416547
2213	17	-0.0290656544475425
2213	18	0.024689880096801176
2213	19	-0.00372677162754087
2213	20	0.09519010242822645
2213	21	-0.5085344011430237
2213	22	-0.9854690984838186
2213	23	-0.18521525400564798
2213	24	-0.04386244881089141
2213	25	-0.6739863450607686
2214	1	-0.2976538178332504
2214	2	-0.4980534060381281
2214	3	-0.46521368506701677
2214	4	-0.6163884799742902
2214	5	-0.295776745378582
2214	6	-0.2519116330603879
2214	7	-0.6602681830478687
2214	8	-0.23972322229398202
2214	9	-0.19870463918938697
2214	10	-2.189539525576066
2214	11	-1.0037358407535564
2214	12	-0.5646071701236011
2214	13	-0.38461357407194185
2214	14	-0.8814782203949534
2214	15	-0.722811275992072
2214	16	-0.8393759243257009
2214	17	-0.7386012400919384
2214	18	-0.6125185649176785
2214	19	-1.5646873958643326
2214	20	-2.564732175175898
2214	21	-1.9285598222033329
2214	22	-2.024577978958753
2214	23	-2.5940181601474066
2214	24	-1.6870037545778658
2214	25	-2.110401053947392
2215	1	-0.25352616456214605
2215	2	-0.33851023965649674
2215	3	-0.9186210623367757
2215	4	-0.33216901757909767
2215	5	-0.13227932726184122
2215	6	-0.2262286553435473
2215	7	-0.22087428044704183
2215	8	-0.24579960524231836
2215	9	-0.19904122996436513
2215	10	-0.26723389090918204
2215	11	-0.6150208042389664
2215	12	-0.47256576374062575
2215	13	-0.3568635002833531
2215	14	-0.2567113250149262
2215	15	-0.5497465073791901
2215	16	-0.46345687830347515
2215	17	-0.2212975558280874
2215	18	-0.2508637092088648
2215	19	-2.4239488962331066
2215	20	-2.4690895985069545
2215	21	-3.2851261017990723
2215	22	-2.2943507277554502
2215	23	-3.5683510329045234
2215	24	-2.3172346041478136
2215	25	-1.540984764207792
2216	1	-1.360349854521214
2216	2	-1.0073467222144643
2216	3	-0.7111688639414702
2216	4	-0.6979399493443452
2216	5	-1.8339949751052345
2216	6	-1.4061252157663944
2216	7	-0.9606892099507576
2216	8	-5.1160182813102395
2216	9	-3.9345566939926115
2216	10	-1.923750510285572
2216	11	-0.9722488183169844
2216	12	-1.2259684084162998
2216	13	-1.8304239271869989
2216	14	-3.248807440625157
2216	15	-1.8358281063180115
2216	16	-2.8044636946427346
2216	17	-2.4481989817916556
2216	18	-2.357290891130738
2216	19	-1.5183210309828326
2216	20	-0.7723888203116047
2216	21	-0.6993550220652551
2216	22	-0.5170533307373972
2216	23	-0.8254348253580801
2216	24	-0.43164712477093303
2216	25	-0.0803937109126096
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	4.0
7	55.0
8	258.0
9	771.0
10	1620.0
11	3366.0
12	6166.0
13	9412.0
14	13186.0
15	17084.0
16	20171.0
17	21584.0
18	22789.0
19	23199.0
20	23652.0
21	25422.0
22	26974.0
23	28184.0
24	30272.0
25	32761.0
26	35230.0
27	39793.0
28	45799.0
29	53866.0
30	64706.0
31	80623.0
32	108417.0
33	153020.0
34	221967.0
35	352892.0
36	608373.0
37	1952397.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.780281275603954	20.69348933682276	13.45102535139564	23.075204036177645
2	14.987602685455176	20.899714971124297	40.28466103236723	23.828021311053295
3	19.068431590158617	25.955237291569055	28.508319838735936	26.46801127953639
4	12.68491517688922	16.795801149136157	36.72301821735754	33.79626545661708
5	14.113773973215618	37.396923872301926	33.87429037293326	14.6150117815492
6	32.61570662099861	36.95417214342891	16.61854074450168	13.811580491070796
7	26.860506380199663	32.51719925288493	22.546379086344672	18.075915280570733
8	24.41201569979339	37.650229796108455	20.389602192046624	17.548152312051535
9	24.19405542488143	17.849207443827726	21.632353836538474	36.324383294752366
10	15.599364812711697	30.432305088705046	31.87151622386624	22.09681387471701
11	32.744844656285565	26.439172162883512	21.3943160751296	19.42166710570132
12	21.352626679370513	27.898810579978324	30.524421356180376	20.22414138447079
13	26.623324177251085	23.96671006548591	26.06600679586244	23.343958961400567
14	20.59933051523488	23.27918309936158	27.302627491619013	28.818858893784526
15	24.29572258282726	29.643258223828497	23.03479643245798	23.026222760886263
16	22.303171096778556	31.048830511595725	25.298842750846866	21.34915564077885
17	21.12034891306374	30.10620227762694	27.881011698776692	20.892437110532626
18	21.340113416119465	29.560781551826153	28.561264411548574	20.537840620505808
19	21.567857073629686	28.38311213563015	29.058807624847105	20.99022316589306
20	22.99237077534791	27.7404324055666	27.802634194831015	21.464562624254473
21	22.85666179792578	28.675723204495323	27.382302769362692	21.0853122282162
22	21.699757196018144	28.23464867285975	28.121292066355647	21.944302064766465
23	21.35111878216017	28.723217994026196	28.266727018147726	21.65893620566591
24	21.656432585407508	28.18709270663595	27.908537159667823	22.247937548288718
25	22.604805481504506	27.372655437472353	26.76454854096185	23.257990540061286
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4008.0
1	4008.0
2	4516.0
3	5024.0
4	5024.0
5	5024.0
6	6555.0
7	8086.0
8	8086.0
9	8086.0
10	11053.0
11	14020.0
12	14020.0
13	14020.0
14	22098.0
15	30176.0
16	30176.0
17	30176.0
18	47829.0
19	65482.0
20	65482.0
21	65482.0
22	94190.5
23	122899.0
24	122899.0
25	122899.0
26	162350.0
27	201801.0
28	201801.0
29	201801.0
30	246399.5
31	290998.0
32	290998.0
33	290998.0
34	335778.5
35	380559.0
36	380559.0
37	380559.0
38	417741.0
39	454923.0
40	454923.0
41	454923.0
42	461110.0
43	467297.0
44	467297.0
45	467297.0
46	504581.0
47	541865.0
48	541865.0
49	541865.0
50	503732.5
51	465600.0
52	465600.0
53	465600.0
54	408366.5
55	351133.0
56	351133.0
57	351133.0
58	304877.0
59	258621.0
60	258621.0
61	258621.0
62	218437.5
63	178254.0
64	178254.0
65	178254.0
66	139302.0
67	100350.0
68	100350.0
69	100350.0
70	75015.0
71	49680.0
72	49680.0
73	49680.0
74	35647.5
75	21615.0
76	21615.0
77	21615.0
78	14757.0
79	7899.0
80	7899.0
81	7899.0
82	5338.0
83	2777.0
84	2777.0
85	2777.0
86	1735.5
87	694.0
88	694.0
89	694.0
90	442.0
91	190.0
92	190.0
93	190.0
94	112.0
95	34.0
96	34.0
97	34.0
98	31.0
99	28.0
100	28.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02974642477546668
2	0.01394130684965481
3	0.019830949850311117
4	0.020303115322937576
5	0.0192096794915921
6	0.004821057983659596
7	2.48508143487608E-5
8	2.733589578363688E-4
9	1.988065147900864E-4
10	7.455244304628241E-5
11	8.200768735091064E-4
12	8.697785022066282E-4
13	0.0023359765487835156
14	5.715687300214984E-4
15	0.0016401537470182128
16	0.0018389602618082994
17	0.0021868716626909502
18	0.0017395570044132563
19	2.733589578363688E-4
20	3.230605865338904E-4
21	0.07763394402552874
22	0.0763914033080907
23	0.07760909321117998
24	9.94032573950432E-4
25	0.21781738776688844
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4024013.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.01853937872062
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.98473253240773	52.00490481854788
2	12.879578955578571	16.748228230092536
3	3.7653326219942116	7.344492820713359
4	1.4004325994313183	3.6421632845347705
5	0.6666994641298282	2.1673912681148586
6	0.36652215820733525	1.4298441225946377
7	0.2156899175314647	0.9816690377628778
8	0.13766571033833117	0.7160658726985865
9	0.10234740413347955	0.5989030853366227
>10	0.42999886760076234	4.991752957275689
>50	0.02874919109903646	1.298441223537444
>100	0.018939221163214705	2.3336286806667133
>500	0.0013476451295528298	0.5550222533480255
>1k	0.0014246532635159195	1.9237264757048744
>5k	4.2354556482905473E-4	1.7491335650366695
>10k+	1.1551242677156037E-4	1.5146323040345158
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	25436	0.6321053137750798	No Hit
CCCATGTACTCTGCGTTGATACCAC	23399	0.581484204946654	No Hit
GAGTACATGGGAAGCAGTGGTATCA	11666	0.2899096001926435	No Hit
GCGTTGATACCACTGCTTCCCATGT	8214	0.20412458906072123	No Hit
CATGTACTCTGCGTTGATACCACTG	8196	0.20367727440244354	No Hit
GTATCAACGCAGAGTACATGGGAAG	7637	0.18978566918148623	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	6895	0.17134636493470573	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5900	0.14661980465768873	No Hit
ATTCCATTCCATTCCATTCCATTCC	5803	0.14420927566585895	No Hit
TATCAACGCAGAGTACATGGGAAGC	5730	0.1423951662183994	No Hit
ACTCTGCGTTGATACCACTGCTTCC	5560	0.13817052777911007	No Hit
GGTATCAACGCAGAGTACATGGGAA	5428	0.13489022028507364	No Hit
GTGGTATCAACGCAGAGTACATGGG	5402	0.13424409911200585	No Hit
GAATGGAATGGAATGGAATGGAATG	5103	0.12681370562172636	No Hit
GCTTCCCATGTACTCTGCGTTGATA	4772	0.11858808607228655	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	4.9701628697521605E-5	0.0	0.0	0.0
5	0.0	4.9701628697521605E-5	0.0	0.0	0.0
6	0.0	4.9701628697521605E-5	0.0	0.0	0.0
7	0.0	4.9701628697521605E-5	0.0	0.0	0.0
8	0.0	4.9701628697521605E-5	0.0	0.0	0.0
9	0.0	4.9701628697521605E-5	0.0	0.0	0.0
10	0.0	4.9701628697521605E-5	0.0	0.0	0.0
11	0.0	4.9701628697521605E-5	0.0	2.4850814348760803E-5	0.0
12	0.0	4.9701628697521605E-5	0.0	2.4850814348760803E-5	0.0
13	0.0	4.9701628697521605E-5	0.0	4.9701628697521605E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTATA	50	0.0015010987	13.299026	2
GACCGCG	100	1.941713E-6	11.395198	7
CTAGACG	85	6.609034E-4	10.056961	4
TAGGACC	255	0.0	10.056961	4
CTGATCG	85	6.623396E-4	10.054587	9
ATTAGAC	335	0.0	9.640605	3
CTAGGAC	395	0.0	9.378593	3
GTCCTAG	355	0.0	9.36784	1
TCTGTCG	195	1.6989361E-9	9.252511	8
GTCTAGG	380	0.0	9.251622	1
AGGACCG	145	1.7130133E-6	9.170487	5
TACACTG	910	0.0	9.08051	5
TAGGACG	95	0.0018278453	8.998334	4
GTCCGGA	85	0.007428349	8.942744	1
CGCGCGA	85	0.00746403	8.937522	12
CTACACT	640	0.0	8.904601	4
GCTACAC	390	0.0	8.768152	3
ACGGTAC	185	6.596383E-8	8.728657	3
GGACCGT	120	1.773104E-4	8.704666	6
TTAAGAC	350	0.0	8.684646	3
>>END_MODULE