FastQCFastQC Report
Wed 25 May 2016
SRR1294689_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294689_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4024013
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC253860.6308627730576417No Hit
CCCATGTACTCTGCGTTGATACCAC248280.6169960186510332No Hit
GTATCAACGCAGAGTACTTTTTTTT188530.4685124029171874No Hit
GAGTACATGGGAAGCAGTGGTATCA132690.32974545559370705No Hit
GGTATCAACGCAGAGTACTTTTTTT132290.32875142301975663No Hit
TATCAACGCAGAGTACTTTTTTTTT123470.3068330047641496No Hit
CATGTACTCTGCGTTGATACCACTG94100.23384616302183914No Hit
GCGTTGATACCACTGCTTCCCATGT92660.23026764575561756No Hit
ACGCAGAGTACATGGGAAGCAGTGG87030.21627663727726526No Hit
GTATCAACGCAGAGTACATGGGAAG83100.20651026723820226No Hit
ACGCAGAGTACTTTTTTTTTTTTTT73660.18305109849297208No Hit
ATTCCATTCCATTCCATTCCATTCC69560.17286226460998014No Hit
TATCAACGCAGAGTACATGGGAAGC67120.16679866590888248No Hit
ACTCTGCGTTGATACCACTGCTTCC64560.16043685743559974No Hit
GGTATCAACGCAGAGTACATGGGAA61120.15188817729962603No Hit
GTGGTATCAACGCAGAGTACATGGG57000.14164964178793657No Hit
GAATGGAATGGAATGGAATGGAATG56810.14117747631531014No Hit
GCTTCCCATGTACTCTGCGTTGATA52290.12994490822967023No Hit
CAGTGGTATCAACGCAGAGTACATG44980.11177896294072608No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC752.086909E-411.39328
TATTCCG3100.010.7423695
TCTACGC1105.941376E-610.3796393
TAGGACT4400.010.3796394
CGCCCTA1951.4551915E-1110.2245416
CCCTACG750.002615173310.1491062
TCGGAAC1502.4720066E-810.12716413
ACTCGGA1701.5788828E-910.05257311
GGACCGT951.6531575E-49.9952786
TAGACAG6350.09.8892625
CTCGGAA1951.6552804E-109.73741512
GTCCTAC2450.09.7132011
CCTACAC4050.09.63213353
TACGCAG1106.727428E-59.5146695
TATACCG1106.727428E-59.5146695
TACACCG1401.0377389E-69.5146695
TACACTG8200.09.5146685
ACCGATC800.0045403719.4943348
GACTGCG1304.275307E-69.4943347
CGAAAAC800.0045408369.49421618
Produced by FastQC (version 0.11.5)