FastQCFastQC Report
Wed 25 May 2016
SRR1294688_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294688_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5986192
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1453202.427586686160417No Hit
GTACATGGGAAGCAGTGGTATCAAC1445972.415508891128116No Hit
GAGTACATGGGAAGCAGTGGTATCA629551.0516702437877035No Hit
GCGTTGATACCACTGCTTCCCATGT511850.8550510909105488No Hit
CATGTACTCTGCGTTGATACCACTG510190.8522780425352211No Hit
GTATCAACGCAGAGTACATGGGAAG456970.7633734434177855No Hit
ACGCAGAGTACATGGGAAGCAGTGG372460.6221985529364912No Hit
ACTCTGCGTTGATACCACTGCTTCC323060.5396753061044484No Hit
TATCAACGCAGAGTACATGGGAAGC311740.5207651207979964No Hit
GCTTCCCATGTACTCTGCGTTGATA299940.5010530901781968No Hit
GGTATCAACGCAGAGTACATGGGAA242750.40551656211494713No Hit
GTGGTATCAACGCAGAGTACATGGG211480.35327968097247797No Hit
CAGTGGTATCAACGCAGAGTACATG206020.34415869053314696No Hit
ATACCACTGCTTCCCATGTACTCTG189590.3167121936616801No Hit
GTACTCTGCGTTGATACCACTGCTT171240.2860583155368221No Hit
GCAGTGGTATCAACGCAGAGTACAT168110.2808296158893667No Hit
CTGCTTCCCATGTACTCTGCGTTGA151640.2533162985751209No Hit
GGGAAGCAGTGGTATCAACGCAGAG149150.24915672601212926No Hit
CATGGGAAGCAGTGGTATCAACGCA147970.24718552295014928No Hit
CCATGTACTCTGCGTTGATACCACT144250.24097122177170394No Hit
TACCACTGCTTCCCATGTACTCTGC142860.23864921138513434No Hit
GTACATGGTAAGCAGTGGTATCAAC139280.23266878175641545No Hit
GATACCACTGCTTCCCATGTACTCT136690.22834215808647634No Hit
ACATGGGAAGCAGTGGTATCAACGC128770.2151117104162379No Hit
CTTCCCATGTACTCTGCGTTGATAC119440.19952584213804034No Hit
GCAGAGTACATGGGAAGCAGTGGTA116680.1949152315862906No Hit
CCACTGCTTCCCATGTACTCTGCGT110480.18455806295554839No Hit
AAGCAGTGGTATCAACGCAGAGTAC106350.1776588522386185No Hit
GTTGATACCACTGCTTCCCATGTAC104810.17508626519162765No Hit
ACCATGTACTCTGCGTTGATACCAC103580.17303153657617398No Hit
ATGGGAAGCAGTGGTATCAACGCAG96930.1619226379641682No Hit
TGATACCACTGCTTCCCATGTACTC89700.1498448429318672No Hit
ATCAACGCAGAGTACATGGGAAGCA89470.14946062538588806No Hit
TTCCCATGTACTCTGCGTTGATACC87760.14660405145708658No Hit
GTACATGGGTAAGCAGTGGTATCAA80420.1343425002071434No Hit
CCCCATGTACTCTGCGTTGATACCA69940.1168355441990501No Hit
GGTAAGCAGTGGTATCAACGCAGAG67910.11344440672801674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAC1657.8216544E-1110.96215619
TCGACCG803.7891028E-410.68299
CGCAAAA3350.010.4924042
GATACGA2001.8189894E-1210.45633218
TAGACTG4600.010.11766055
GTTAGAC1554.080175E-89.8050983
GTATCGA1752.5520421E-99.77734918
CGCCCTA1201.6905824E-59.50559816
CGTAAGA800.00451910089.4998792
ATACGAC1951.6243575E-99.27567119
CTAGGAC4000.09.2612213
GTTCTAG5350.09.0583821
CCGGTCA1901.0679287E-88.9961269
TAGGACG850.0074503988.9396434
TAGGACA4250.08.9396424
TGATACG2401.0004442E-108.71361117
GTACAAA9450.08.6477041
ACGCAAA3900.08.5278131
TGCTACG1451.6913615E-58.5171332
AAGCGGT3750.08.35633210
Produced by FastQC (version 0.11.5)