##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294688_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5986192
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.265098413148124	33.0	33.0	33.0	27.0	33.0
2	31.22245193605551	33.0	33.0	33.0	27.0	33.0
3	31.254509210529832	33.0	33.0	33.0	27.0	33.0
4	34.67885276649997	37.0	37.0	37.0	27.0	37.0
5	34.94576685812951	37.0	37.0	37.0	33.0	37.0
6	35.20648970163336	37.0	37.0	37.0	33.0	37.0
7	35.21040187150696	37.0	37.0	37.0	33.0	37.0
8	35.10438522519826	37.0	37.0	37.0	33.0	37.0
9	34.84652062613428	37.0	37.0	37.0	33.0	37.0
10	34.91502210420247	37.0	37.0	37.0	33.0	37.0
11	35.17644338838447	37.0	37.0	37.0	33.0	37.0
12	35.11487052202803	37.0	37.0	37.0	33.0	37.0
13	35.01792608723542	37.0	37.0	37.0	33.0	37.0
14	36.23739983615627	40.0	37.0	40.0	27.0	40.0
15	36.50374645517551	40.0	37.0	40.0	33.0	40.0
16	36.47963546775647	40.0	37.0	40.0	33.0	40.0
17	36.47615295332993	40.0	37.0	40.0	33.0	40.0
18	36.46383276714145	40.0	37.0	40.0	33.0	40.0
19	36.48207959250221	40.0	37.0	40.0	33.0	40.0
20	36.39647274928703	40.0	37.0	40.0	27.0	40.0
21	36.328557286502004	40.0	37.0	40.0	27.0	40.0
22	36.23439809481553	40.0	37.0	40.0	27.0	40.0
23	35.84540405653544	37.0	37.0	40.0	27.0	40.0
24	36.17530109291516	40.0	37.0	40.0	27.0	40.0
25	35.09420145561653	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5495519353137759
1101	2	0.6840926186429357
1101	3	0.49555359791593645
1101	4	0.6971248045762479
1101	5	0.6290666123952633
1101	6	0.5630961771772007
1101	7	0.6020645793989488
1101	8	0.648941345393176
1101	9	0.882688893823655
1101	10	0.8766113014716126
1101	11	0.5978509021218912
1101	12	0.658368387867327
1101	13	0.7043906929995885
1101	14	1.4269068545581973
1101	15	1.1096844371283225
1101	16	1.0449382438394892
1101	17	1.0357280934993298
1101	18	1.0730106185424901
1101	19	1.0370598398412199
1101	20	1.137666522385004
1101	21	1.1321974253872114
1101	22	1.1725384311551323
1101	23	1.5435279525144665
1101	24	1.2942778710884255
1101	25	1.2196854406466215
1102	1	0.3963639606273688
1102	2	0.56815546554623
1102	3	0.4350526542205806
1102	4	0.46425352643086626
1102	5	0.45323293590324454
1102	6	0.47954506005844877
1102	7	0.47801256647996837
1102	8	0.572228588909411
1102	9	0.7428618823953386
1102	10	0.8343550494360699
1102	11	0.538160408474937
1102	12	0.5615093150135735
1102	13	0.5951973614859156
1102	14	1.2967499356072878
1102	15	0.9843854020482112
1102	16	0.7003329997433454
1102	17	0.9038351243526179
1102	18	0.8739125186765264
1102	19	0.9277706239069801
1102	20	0.9953838903618291
1102	21	1.0147723215193736
1102	22	1.0832147947723172
1102	23	1.3967824368432886
1102	24	1.1724427431051225
1102	25	1.060017403892445
1103	1	0.2792051468927852
1103	2	0.489194978681887
1103	3	0.29979278894377615
1103	4	0.11133907675414889
1103	5	0.32809209121772653
1103	6	0.2982395722449809
1103	7	0.24376143294301755
1103	8	0.4240683358262274
1103	9	0.5823057203667759
1103	10	0.6533100848753222
1103	11	0.4123339838887503
1103	12	0.4057318213426484
1103	13	0.4079286515832905
1103	14	1.0816083252101762
1103	15	0.8316087935966436
1103	16	0.5790949980813807
1103	17	0.5809953244613837
1103	18	0.6839940104655966
1103	19	0.6942335545317206
1103	20	0.8651518518956749
1103	21	0.9577636613580509
1103	22	0.8730838391705547
1103	23	1.336061849155179
1103	24	0.9684165506183717
1103	25	0.8749912514518243
1104	1	0.4012246129573285
1104	2	0.5339553991479775
1104	3	0.40659555149562365
1104	4	0.5051419310600949
1104	5	0.3366723767561268
1104	6	0.32098678604999265
1104	7	0.3593684180265839
1104	8	0.5205624371438304
1104	9	0.6489846542834528
1104	10	0.6704906649945741
1104	11	0.4938966421024915
1104	12	0.5016092873528493
1104	13	0.5262813932385839
1104	14	1.0593249387206853
1104	15	0.8698625917563376
1104	16	0.7879115725710477
1104	17	0.8060023972536001
1104	18	0.7591115366374339
1104	19	0.7962373426965144
1104	20	0.8832656568348227
1104	21	0.9592227299739591
1104	22	0.9961658251759573
1104	23	1.3627311433081601
1104	24	1.1322885418773296
1104	25	0.9000128349710366
1105	1	0.2156420116370512
1105	2	0.33386991764778884
1105	3	0.32337611157105073
1105	4	0.31460532501152727
1105	5	0.25704762547957927
1105	6	0.24125975720980364
1105	7	0.31574188155543936
1105	8	0.35744435587699286
1105	9	-1.673346764675209
1105	10	-0.07322945911180057
1105	11	0.08585761043674012
1105	12	0.2863767185474657
1105	13	0.34752744304568495
1105	14	-1.6044990965598416
1105	15	-0.21618008295687474
1105	16	-0.023359331543282735
1105	17	0.22497942929391002
1105	18	0.26476861432128374
1105	19	0.4211728806003663
1105	20	0.4850611676433303
1105	21	0.6168397403274852
1105	22	0.5953942121001887
1105	23	1.0154003280449047
1105	24	0.712651140986523
1105	25	0.48493355867616117
1106	1	-0.011851539206379158
1106	2	0.011689869605906011
1106	3	0.07008296652716339
1106	4	-1.3335021233375102
1106	5	-0.3517633705892038
1106	6	-0.11699886169279239
1106	7	-0.006722034901791574
1106	8	0.08117906324456214
1106	9	0.3315085762035679
1106	10	0.41463984315659985
1106	11	0.26957870490529956
1106	12	0.09102120459026253
1106	13	0.13917203415822854
1106	14	0.6549447379795126
1106	15	0.3488397993741472
1106	16	0.20291447676110153
1106	17	0.12396283512333639
1106	18	0.2173130854331191
1106	19	0.21243923838937917
1106	20	0.3174005873610426
1106	21	0.4739213218853777
1106	22	0.5540605305299096
1106	23	0.7098642585046662
1106	24	0.3598239708739399
1106	25	0.3751429559294408
1107	1	0.3284433569641294
1107	2	0.2713814207517231
1107	3	0.2795381617235613
1107	4	0.37213765994559367
1107	5	0.3313666060980367
1107	6	0.2081635668791506
1107	7	0.28729212612169164
1107	8	0.3087443478506202
1107	9	0.46972426358206576
1107	10	0.4633822825338356
1107	11	0.3088511359642325
1107	12	0.27282448086161537
1107	13	0.24624670745352972
1107	14	0.8441665404454142
1107	15	0.5709412626299653
1107	16	0.5676572965290774
1107	17	0.6137778997026615
1107	18	0.6649938404498883
1107	19	0.5724363447323739
1107	20	0.7016841682446397
1107	21	0.6734707211646764
1107	22	0.6411902552484179
1107	23	0.9574117697426345
1107	24	0.8129064171490938
1107	25	0.6800202216489026
1108	1	0.35058197556074333
1108	2	0.34141657149480054
1108	3	0.4347403351058645
1108	4	0.5771441620139228
1108	5	0.4673292327766987
1108	6	0.32332144199482826
1108	7	0.3409583884513765
1108	8	0.37815389395356647
1108	9	0.11179691685537563
1108	10	0.4042357746561578
1108	11	0.25589278506951985
1108	12	0.2847365749585933
1108	13	0.35725322044369534
1108	14	-1.1897322914340336
1108	15	-0.0759493634038293
1108	16	0.2942714801082147
1108	17	0.43865391623123884
1108	18	0.6123407673243193
1108	19	0.5267749551670988
1108	20	0.6294652833260912
1108	21	0.6277309976551422
1108	22	0.7169386318511215
1108	23	1.0299985715090543
1108	24	0.8626774047746224
1108	25	0.6964891893971767
1109	1	0.36481481075933786
1109	2	0.21206129051215328
1109	3	0.3029053813305147
1109	4	0.47751380421748024
1109	5	0.4817736765392624
1109	6	0.29776259366455093
1109	7	0.22096116095541873
1109	8	0.35920914290399253
1109	9	0.5010920441829327
1109	10	0.45612918376308187
1109	11	0.30824197083908444
1109	12	0.32395766542730087
1109	13	0.2574618605781893
1109	14	0.7125500898982793
1109	15	0.37892013263980573
1109	16	0.325978545940913
1109	17	0.3451125096288834
1109	18	0.44584242151915277
1109	19	0.3992290978752351
1109	20	0.5173555320784402
1109	21	0.5558556600559541
1109	22	0.6505740381142218
1109	23	1.1222073555646475
1109	24	0.7722104819547653
1109	25	0.6311285316932143
1110	1	0.36279013690679207
1110	2	0.2653795796453089
1110	3	0.3340160479588974
1110	4	0.5673689809615325
1110	5	0.44497725062051074
1110	6	0.3084375175881391
1110	7	0.31194797533117935
1110	8	0.3286156715411721
1110	9	0.45222047471770566
1110	10	0.4792314267835778
1110	11	0.3229295726424084
1110	12	0.23903296839343824
1110	13	0.18371349479486554
1110	14	0.620709428595994
1110	15	0.47683600372089074
1110	16	0.3963963057985538
1110	17	0.4108167721632654
1110	18	0.5215596367644224
1110	19	0.43475126661650165
1110	20	0.6232268189010526
1110	21	0.6237621254863654
1110	22	0.6996543604346144
1110	23	0.8890592637103154
1110	24	0.7141534347965788
1110	25	0.6761022406496622
1111	1	0.18978406372701784
1111	2	0.05374685530679457
1111	3	0.23791221718641964
1111	4	0.5316379159377007
1111	5	0.35062254848640606
1111	6	0.20259811841120268
1111	7	0.15413996446354616
1111	8	0.3096576370503499
1111	9	0.29244772629878213
1111	10	0.16560736282521304
1111	11	0.1622374541766618
1111	12	0.1642052716753497
1111	13	0.1334022580534011
1111	14	-1.8320193670454472
1111	15	-0.54722673964514
1111	16	-0.10337205583695663
1111	17	0.12659081828567054
1111	18	0.2342254933611585
1111	19	0.21291992096002588
1111	20	0.33245243971990135
1111	21	0.33981417175137096
1111	22	0.1745790438609376
1111	23	-2.0835039212649775
1111	24	-0.4346385957523182
1111	25	-0.02843154690830829
1112	1	-0.0873402684214426
1112	2	-0.1914321886989221
1112	3	0.02448954889736399
1112	4	0.1682889369663556
1112	5	-0.6239916007330635
1112	6	-0.8414186219447828
1112	7	-0.9329232960177762
1112	8	-0.7263938383214992
1112	9	-0.517994332923351
1112	10	-0.8049557081219234
1112	11	-0.7599836005121858
1112	12	-0.7460151910948056
1112	13	-0.8136462210409405
1112	14	-0.5418428339273262
1112	15	-0.8154776178340342
1112	16	-0.869133766697523
1112	17	-0.8858881790670665
1112	18	-0.9171480336556002
1112	19	-0.8104495513307768
1112	20	-0.924341060449116
1112	21	-1.7465710143641573
1112	22	-1.732193223090249
1112	23	-1.3210615561352839
1112	24	-1.6348263475535134
1112	25	-1.8309971704011758
1113	1	-0.07519869409291857
1113	2	-0.21676401585781946
1113	3	-0.14523039793139958
1113	4	0.17831379612660214
1113	5	-0.0235924827128855
1113	6	-0.18669357615119253
1113	7	-0.20426799362534354
1113	8	-0.020620523642399746
1113	9	0.1090355511673664
1113	10	-0.21670744906720074
1113	11	-0.28812815684763393
1113	12	-0.15635612235602991
1113	13	-0.24560755204522877
1113	14	-0.17856836701264456
1113	15	-0.30502951880276186
1113	16	-0.163615203036926
1113	17	-0.14133296627441894
1113	18	-0.17126966907461139
1113	19	-0.3087913940642011
1113	20	-0.7828145811804035
1113	21	-0.7377075540527969
1113	22	-0.2608439438481156
1113	23	-0.4735658138695271
1113	24	-0.14872074603496088
1113	25	-1.3286820188749928
1114	1	-0.9896083624186147
1114	2	-0.6697314823159672
1114	3	-0.49189051305447506
1114	4	-1.602626568656504
1114	5	-0.7206560867335483
1114	6	-0.573847200098129
1114	7	-0.5051991229308186
1114	8	-0.43356907496602304
1114	9	-0.37050614251820235
1114	10	-0.441349779445801
1114	11	-0.471493856684134
1114	12	-0.6307228068099278
1114	13	-0.5801604623602259
1114	14	-0.5840286661150031
1114	15	-1.299109847275524
1114	16	-0.8589550043744509
1114	17	-0.8356485673061158
1114	18	-0.8527686524578542
1114	19	-0.8473724945542003
1114	20	-2.0376025887098663
1114	21	-6.910759969491117
1114	22	-8.06697877146945
1114	23	-8.17633804408791
1114	24	-5.971634825919253
1114	25	-7.375709215845941
1115	1	-0.989919888162369
1115	2	-1.0251031170680314
1115	3	-0.6831397980187539
1115	4	-0.5808701470544051
1115	5	-0.6227337450116082
1115	6	-0.826507396181114
1115	7	-1.088972471870278
1115	8	-0.6959542438868809
1115	9	-2.8175948668820396
1115	10	-1.6736053812618508
1115	11	-1.2149601575443114
1115	12	-1.0219754532347949
1115	13	-1.451670216585228
1115	14	-1.0908395614087425
1115	15	-2.0711239739483744
1115	16	-1.3371432285106692
1115	17	-3.18203481272333
1115	18	-2.7754546236072386
1115	19	-2.1910214914193347
1115	20	-1.823816316591632
1115	21	-1.4542699113113216
1115	22	-0.9204654392162652
1115	23	-0.36371196081992707
1115	24	-1.3665630077541309
1115	25	-1.0817440261536646
1116	1	-1.6670478998739142
1116	2	-0.8744468604925792
1116	3	-0.7035015433987653
1116	4	-0.3664535000377782
1116	5	-0.3892837029807268
1116	6	-0.4728328847103498
1116	7	-0.9105788026471515
1116	8	-1.95402283721792
1116	9	-0.9849464347734269
1116	10	-2.5756172660834977
1116	11	-1.1607403173548505
1116	12	-0.8272775459999195
1116	13	-0.8825562594189549
1116	14	-1.272084367372301
1116	15	-1.3303600594667913
1116	16	-0.9025648070986705
1116	17	-0.7306401954247903
1116	18	-1.4468870204553141
1116	19	-1.000841324674191
1116	20	-1.2054631322004
1116	21	-0.794343583437076
1116	22	-0.30497012684458724
1116	23	-0.215277097863094
1116	24	-0.29921807986229965
1116	25	-0.2594790836439813
1201	1	0.33264554785005274
1201	2	0.36412765725349416
1201	3	0.2832407337632503
1201	4	0.3640614094411845
1201	5	0.37553137954143523
1201	6	0.31028578249807026
1201	7	0.3575509830915635
1201	8	0.37643849373950644
1201	9	0.6493412659243916
1201	10	0.6364626739717352
1201	11	0.44190442059194623
1201	12	0.4350323851698903
1201	13	0.4348083579833144
1201	14	1.1657856457424316
1201	15	0.8988812004566427
1201	16	0.8674643891866864
1201	17	0.7912869943704877
1201	18	0.6992407123484412
1201	19	0.6881546755035046
1201	20	0.8330506540165814
1201	21	0.8350000519388843
1201	22	0.9058675806913428
1201	23	0.8281177379319047
1201	24	0.86133092037106
1201	25	0.8711424281748066
1202	1	-0.05205875803487814
1202	2	0.23012949802021154
1202	3	0.11125040817671206
1202	4	0.1337623143793607
1202	5	0.15287750680460022
1202	6	0.1546773868975393
1202	7	0.13630792716359252
1202	8	0.2814652428093254
1202	9	0.3234568734356742
1202	10	0.5246131838079009
1202	11	0.26452591855145613
1202	12	0.3027258930818135
1202	13	0.3524387800334523
1202	14	0.6173275462554955
1202	15	0.5936600445270699
1202	16	0.5906442103378637
1202	17	0.46297839574670263
1202	18	0.4106476391217484
1202	19	0.4785286910574271
1202	20	0.5622820194833906
1202	21	0.6693817095116614
1202	22	0.6153250997577615
1202	23	0.9358271508529299
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2211	5	0.3242337607447823
2211	6	0.18504974597950508
2211	7	0.15836422749995194
2211	8	0.2770214874343111
2211	9	0.38861320200184224
2211	10	0.34229615537688574
2211	11	0.20466718822451924
2211	12	0.1936079558911885
2211	13	0.1637986695688909
2211	14	0.3819738068387011
2211	15	0.22228373333032891
2211	16	0.19496327198771013
2211	17	0.2836468244126138
2211	18	0.22279710477683068
2211	19	0.26341042661682224
2211	20	0.29360917474087955
2211	21	0.3890681620413048
2211	22	0.4356695497878462
2211	23	0.5200629128253809
2211	24	0.5367710990858328
2211	25	0.5614129513343613
2212	1	-0.2805233784969623
2212	2	-0.2916957450580533
2212	3	-0.23513650048064605
2212	4	-0.5363992285189738
2212	5	-0.22186171622610118
2212	6	-0.6108190160363876
2212	7	-0.5659660599842979
2212	8	-0.32799351659602394
2212	9	-0.2618568836694095
2212	10	-0.6129569720434276
2212	11	-0.5117955988173861
2212	12	-0.6478327411531097
2212	13	-0.639396356796496
2212	14	-0.4763502663713268
2212	15	-0.6576439837116865
2212	16	-0.4428335930740417
2212	17	-0.5136488780686932
2212	18	-0.5136916392237083
2212	19	-0.5232078114279233
2212	20	-0.4606894100316836
2212	21	-0.42166734142373485
2212	22	-0.5764068324534293
2212	23	-0.08645767233872448
2212	24	-0.3130128190636299
2212	25	-1.0768550377877517
2213	1	-0.10942013509718862
2213	2	-1.2459083936762383
2213	3	-0.25388593250919556
2213	4	0.028056458152903474
2213	5	0.006497876399265579
2213	6	-0.06919593546940916
2213	7	-0.08332970917446403
2213	8	0.1131541661317641
2213	9	0.09732604090716279
2213	10	-0.44028715067277346
2213	11	-0.11489384088264387
2213	12	-0.0691080350174218
2213	13	-0.37504197220270896
2213	14	-0.04490209698211345
2213	15	-0.1688706524705239
2213	16	-0.16054743935266202
2213	17	-0.035276599060274805
2213	18	0.004529845963787693
2213	19	-0.0017145407185452655
2213	20	0.06372653983229526
2213	21	-0.594886335501279
2213	22	-1.055189168623734
2213	23	-0.22354554510145874
2213	24	-0.051639224224317104
2213	25	-0.7232751370358201
2214	1	-0.2555268495110852
2214	2	-0.4582598886744762
2214	3	-0.45825780704388563
2214	4	-0.545423575422241
2214	5	-0.33450177305466156
2214	6	-0.251821645634827
2214	7	-0.6406663734033273
2214	8	-0.22775449293017402
2214	9	-0.09329522844944194
2214	10	-1.9872358960295884
2214	11	-0.913244628471034
2214	12	-0.5974992957061573
2214	13	-0.41144357031681267
2214	14	-0.8321491193990624
2214	15	-0.6267313868795199
2214	16	-0.8015138466880458
2214	17	-0.629302971962808
2214	18	-0.5759664251492111
2214	19	-1.5519295503021482
2214	20	-2.644129535749215
2214	21	-2.029565861475625
2214	22	-2.190391453083528
2214	23	-2.7453735362981604
2214	24	-1.752406851645702
2214	25	-2.2235273589739037
2215	1	-0.26700009883024123
2215	2	-0.4041155929711451
2215	3	-0.9548495666929995
2215	4	-0.3574861087963015
2215	5	-0.2727566861576918
2215	6	-0.36020830874821996
2215	7	-0.3460650191014736
2215	8	-0.3238694732050149
2215	9	-0.3516916607212224
2215	10	-0.3643548253134554
2215	11	-0.7050863490405916
2215	12	-0.5080254132777
2215	13	-0.4001331597975337
2215	14	-0.3375695256709079
2215	15	-0.7376606335525508
2215	16	-0.6016110177959177
2215	17	-0.4408647665241574
2215	18	-0.5272453135388702
2215	19	-2.4494173648779665
2215	20	-2.6560887575686394
2215	21	-3.5244138219671655
2215	22	-2.671234955142886
2215	23	-3.6164331019650575
2215	24	-2.631278439874599
2215	25	-2.0016279877965886
2216	1	-1.4866388528598904
2216	2	-1.1235900791323665
2216	3	-0.7776142837896138
2216	4	-0.9013182516892826
2216	5	-1.9359774544324964
2216	6	-1.5887615331143365
2216	7	-1.0978129811621926
2216	8	-5.427459571837407
2216	9	-4.272816422597316
2216	10	-2.2093506039445785
2216	11	-1.0789015347879882
2216	12	-1.1545724195459712
2216	13	-1.9124403493119146
2216	14	-3.5798458540403644
2216	15	-1.9662269018768015
2216	16	-3.187833731197223
2216	17	-2.7686267529899737
2216	18	-2.7231857757536773
2216	19	-1.7106895664830901
2216	20	-1.0067278398473505
2216	21	-0.7688682046654307
2216	22	-0.755477280350263
2216	23	-1.1713490257711427
2216	24	-0.6132043874608186
2216	25	-0.41706314714071624
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	79.0
8	365.0
9	1109.0
10	2111.0
11	4052.0
12	6714.0
13	10090.0
14	13550.0
15	18197.0
16	22444.0
17	24069.0
18	25428.0
19	26429.0
20	28178.0
21	30748.0
22	33582.0
23	36575.0
24	40212.0
25	44331.0
26	48297.0
27	55371.0
28	64331.0
29	76912.0
30	93484.0
31	115718.0
32	158001.0
33	227811.0
34	337279.0
35	536920.0
36	935970.0
37	2967832.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.18308298757267	19.12361995123981	12.41748950185399	24.275807559333533
2	14.03980655420756	21.26057541201461	38.18756676723451	26.512051266543317
3	20.69289658280158	27.017406206699395	27.172175198565622	25.117522011933403
4	13.388018087305687	18.079369139800345	34.13301417834773	34.39959859454624
5	13.72187303992218	37.6295008900445	34.248885648245455	14.399740421787863
6	33.512684888945095	33.34582395770807	17.852749738841258	15.288741414505575
7	26.233001521518766	32.09824921769078	22.670696430611862	18.998052830178594
8	26.43211737020263	34.82987892489274	21.115300247121716	17.622703457782915
9	26.417190054590932	17.11934146754204	20.408328612721448	36.05513986514558
10	16.632596451118054	29.661668309621163	31.53303274031613	22.17270249894465
11	31.172576191584305	26.172615449020707	20.60277037222899	22.052037987165992
12	23.954462755839202	28.050663090846793	28.98996731278774	19.00490684052626
13	28.057455766600608	22.05132248730419	24.426051251655732	25.46517049443947
14	21.8902226338389	23.966882230855482	25.954954033910266	28.187941101395353
15	28.79053014591045	25.67813702114025	22.037500737128155	23.493832095821148
16	21.31712406962731	28.761876378414986	28.144540248798787	21.776459303158916
17	24.285868215157087	28.7027912419636	27.257279221923657	19.754061320955657
18	24.809294777826725	26.763782120192026	27.8856365051467	20.541286596834553
19	23.130252420203973	28.923891056199718	28.456062633714485	19.489793889881827
20	24.307328391943432	26.74476334617961	26.6487253118438	22.29918295003316
21	21.84097488749832	28.492450734328845	27.98907718862497	21.67749718954786
22	22.554415970800715	27.774279965145958	24.404314892746253	25.266989171307074
23	20.340787787545967	28.691877450812385	27.103646532980797	23.86368822866085
24	22.211206010163163	31.055438683475977	25.025446336170283	21.707908970190577
25	23.362081320326865	25.6951150745443	23.774633238816346	27.168170366312484
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5936.0
1	5936.0
2	7172.5
3	8409.0
4	8409.0
5	8409.0
6	10589.5
7	12770.0
8	12770.0
9	12770.0
10	14075.0
11	15380.0
12	15380.0
13	15380.0
14	20586.5
15	25793.0
16	25793.0
17	25793.0
18	41168.5
19	56544.0
20	56544.0
21	56544.0
22	87153.5
23	117763.0
24	117763.0
25	117763.0
26	162512.5
27	207262.0
28	207262.0
29	207262.0
30	263470.0
31	319678.0
32	319678.0
33	319678.0
34	376666.0
35	433654.0
36	433654.0
37	433654.0
38	492446.0
39	551238.0
40	551238.0
41	551238.0
42	610118.0
43	668998.0
44	668998.0
45	668998.0
46	945335.0
47	1221672.0
48	1221672.0
49	1221672.0
50	1086850.0
51	952028.0
52	952028.0
53	952028.0
54	745755.5
55	539483.0
56	539483.0
57	539483.0
58	443738.0
59	347993.0
60	347993.0
61	347993.0
62	293899.0
63	239805.0
64	239805.0
65	239805.0
66	189475.5
67	139146.0
68	139146.0
69	139146.0
70	105392.5
71	71639.0
72	71639.0
73	71639.0
74	51889.5
75	32140.0
76	32140.0
77	32140.0
78	22285.0
79	12430.0
80	12430.0
81	12430.0
82	8433.0
83	4436.0
84	4436.0
85	4436.0
86	2924.5
87	1413.0
88	1413.0
89	1413.0
90	929.5
91	446.0
92	446.0
93	446.0
94	275.0
95	104.0
96	104.0
97	104.0
98	68.0
99	32.0
100	32.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030102609471931406
2	0.0141325236477547
3	0.019093941524094115
4	0.020396940158284266
5	0.018342211542830566
6	0.006164185846361092
7	0.0011359475272426944
8	0.001219473080716422
9	5.512686529266018E-4
10	4.176277673686377E-4
11	0.001085832195158458
12	9.856015309899848E-4
13	0.002689522821854027
14	6.682044277898203E-4
15	0.0020547286154536976
16	0.0025057666042118264
17	0.0024556512721275897
18	0.0020213183940642064
19	3.842175459791467E-4
20	3.6751243528440115E-4
21	0.08265688771760077
22	0.07811309760862999
23	0.07946621157490438
24	0.001085832195158458
25	0.22331391976735795
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5986192.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.97419917774868
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.37132858025794	40.45889912052605
2	12.626638415226893	12.872655630463772
3	3.7251622138553895	5.696614819754635
4	1.5419281372698768	3.1439420794787885
5	0.8135790581753869	2.0735770479138678
6	0.44267642876901014	1.35390458708196
7	0.2857850432619103	1.0197364602077899
8	0.20835893676102163	0.8496743954336208
9	0.14584545975854354	0.6690919963412096
>10	0.752637378269396	6.8884096687024154
>50	0.05203447160846319	1.8280590013062603
>100	0.028488187159567867	2.7168550895147887
>500	0.0018014183753532929	0.6626424339623843
>1k	0.002468607791021024	2.858723063282895
>5k	2.6687651794711563E-4	1.0824557013198925
>10k+	0.0010007869423016836	15.824758904709707
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCAC	145320	2.427586686160417	No Hit
GTACATGGGAAGCAGTGGTATCAAC	144597	2.415508891128116	No Hit
GAGTACATGGGAAGCAGTGGTATCA	62955	1.0516702437877035	No Hit
GCGTTGATACCACTGCTTCCCATGT	51185	0.8550510909105488	No Hit
CATGTACTCTGCGTTGATACCACTG	51019	0.8522780425352211	No Hit
GTATCAACGCAGAGTACATGGGAAG	45697	0.7633734434177855	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	37246	0.6221985529364912	No Hit
ACTCTGCGTTGATACCACTGCTTCC	32306	0.5396753061044484	No Hit
TATCAACGCAGAGTACATGGGAAGC	31174	0.5207651207979964	No Hit
GCTTCCCATGTACTCTGCGTTGATA	29994	0.5010530901781968	No Hit
GGTATCAACGCAGAGTACATGGGAA	24275	0.40551656211494713	No Hit
GTGGTATCAACGCAGAGTACATGGG	21148	0.35327968097247797	No Hit
CAGTGGTATCAACGCAGAGTACATG	20602	0.34415869053314696	No Hit
ATACCACTGCTTCCCATGTACTCTG	18959	0.3167121936616801	No Hit
GTACTCTGCGTTGATACCACTGCTT	17124	0.2860583155368221	No Hit
GCAGTGGTATCAACGCAGAGTACAT	16811	0.2808296158893667	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	15164	0.2533162985751209	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	14915	0.24915672601212926	No Hit
CATGGGAAGCAGTGGTATCAACGCA	14797	0.24718552295014928	No Hit
CCATGTACTCTGCGTTGATACCACT	14425	0.24097122177170394	No Hit
TACCACTGCTTCCCATGTACTCTGC	14286	0.23864921138513434	No Hit
GTACATGGTAAGCAGTGGTATCAAC	13928	0.23266878175641545	No Hit
GATACCACTGCTTCCCATGTACTCT	13669	0.22834215808647634	No Hit
ACATGGGAAGCAGTGGTATCAACGC	12877	0.2151117104162379	No Hit
CTTCCCATGTACTCTGCGTTGATAC	11944	0.19952584213804034	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	11668	0.1949152315862906	No Hit
CCACTGCTTCCCATGTACTCTGCGT	11048	0.18455806295554839	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	10635	0.1776588522386185	No Hit
GTTGATACCACTGCTTCCCATGTAC	10481	0.17508626519162765	No Hit
ACCATGTACTCTGCGTTGATACCAC	10358	0.17303153657617398	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	9693	0.1619226379641682	No Hit
TGATACCACTGCTTCCCATGTACTC	8970	0.1498448429318672	No Hit
ATCAACGCAGAGTACATGGGAAGCA	8947	0.14946062538588806	No Hit
TTCCCATGTACTCTGCGTTGATACC	8776	0.14660405145708658	No Hit
GTACATGGGTAAGCAGTGGTATCAA	8042	0.1343425002071434	No Hit
CCCCATGTACTCTGCGTTGATACCA	6994	0.1168355441990501	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	6791	0.11344440672801674	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.6705110694745508E-5	0.0	0.0	0.0
6	0.0	1.6705110694745508E-5	0.0	0.0	0.0
7	0.0	1.6705110694745508E-5	0.0	0.0	0.0
8	0.0	1.6705110694745508E-5	0.0	0.0	0.0
9	0.0	1.6705110694745508E-5	0.0	5.0115332084236524E-5	0.0
10	0.0	1.6705110694745508E-5	0.0	1.0023066416847305E-4	0.0
11	0.0	1.6705110694745508E-5	0.0	1.1693577486321856E-4	0.0
12	0.0	1.6705110694745508E-5	0.0	1.6705110694745508E-4	0.0
13	0.0	1.6705110694745508E-5	0.0	1.8375621764220057E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGAC	165	7.8216544E-11	10.962156	19
TCGACCG	80	3.7891028E-4	10.6829	9
CGCAAAA	335	0.0	10.492404	2
GATACGA	200	1.8189894E-12	10.456332	18
TAGACTG	460	0.0	10.1176605	5
GTTAGAC	155	4.080175E-8	9.805098	3
GTATCGA	175	2.5520421E-9	9.777349	18
CGCCCTA	120	1.6905824E-5	9.505598	16
CGTAAGA	80	0.0045191008	9.499879	2
ATACGAC	195	1.6243575E-9	9.275671	19
CTAGGAC	400	0.0	9.261221	3
GTTCTAG	535	0.0	9.058382	1
CCGGTCA	190	1.0679287E-8	8.996126	9
TAGGACG	85	0.007450398	8.939643	4
TAGGACA	425	0.0	8.939642	4
TGATACG	240	1.0004442E-10	8.713611	17
GTACAAA	945	0.0	8.647704	1
ACGCAAA	390	0.0	8.527813	1
TGCTACG	145	1.6913615E-5	8.517133	2
AAGCGGT	375	0.0	8.356332	10
>>END_MODULE