FastQCFastQC Report
Wed 25 May 2016
SRR1294688_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294688_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5986192
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1610382.690157616060427No Hit
GTACATGGGAAGCAGTGGTATCAAC1370572.289552356489735No Hit
GAGTACATGGGAAGCAGTGGTATCA678271.1330575430925036No Hit
CATGTACTCTGCGTTGATACCACTG589590.9849166214515004No Hit
GCGTTGATACCACTGCTTCCCATGT556120.9290046159561872No Hit
GTATCAACGCAGAGTACATGGGAAG458040.7651608902621232No Hit
ACGCAGAGTACATGGGAAGCAGTGG439260.7337886923773912No Hit
ACTCTGCGTTGATACCACTGCTTCC377770.631068966715401No Hit
TATCAACGCAGAGTACATGGGAAGC344160.5749230896703614No Hit
GCTTCCCATGTACTCTGCGTTGATA321000.5362340533013308No Hit
GGTATCAACGCAGAGTACATGGGAA258590.4319774574554241No Hit
CAGTGGTATCAACGCAGAGTACATG239520.40012081136054434No Hit
GTGGTATCAACGCAGAGTACATGGG222100.3710205085302977No Hit
ATACCACTGCTTCCCATGTACTCTG208820.34883612152767574No Hit
GCAGTGGTATCAACGCAGAGTACAT188400.31472428548900533No Hit
CATGGGAAGCAGTGGTATCAACGCA177410.29636536883548004No Hit
CCATGTACTCTGCGTTGATACCACT172920.2888647741335393No Hit
GGGAAGCAGTGGTATCAACGCAGAG171490.28647594330419074No Hit
GTACTCTGCGTTGATACCACTGCTT170380.28462167601707394No Hit
CTGCTTCCCATGTACTCTGCGTTGA165780.276937325097491No Hit
TACCACTGCTTCCCATGTACTCTGC165330.2761855951162275No Hit
GATACCACTGCTTCCCATGTACTCT156820.26196954591499905No Hit
ACATGGGAAGCAGTGGTATCAACGC154930.25881227999369216No Hit
CTTCCCATGTACTCTGCGTTGATAC140940.2354418301317432No Hit
GCAGAGTACATGGGAAGCAGTGGTA129260.21593026084028044No Hit
CCACTGCTTCCCATGTACTCTGCGT127660.21325744312912115No Hit
GTACATGGTAAGCAGTGGTATCAAC127290.21263935403341558No Hit
AAGCAGTGGTATCAACGCAGAGTAC124780.20844637124903445No Hit
ACCATGTACTCTGCGTTGATACCAC115810.19346188695584773No Hit
GTATCAACGCAGAGTACTTTTTTTT112630.18814966175491865No Hit
ATGGGAAGCAGTGGTATCAACGCAG112600.1880995464228344No Hit
GTTGATACCACTGCTTCCCATGTAC109720.18328847454274771No Hit
ATCAACGCAGAGTACATGGGAAGCA102030.1704422444184884No Hit
TGATACCACTGCTTCCCATGTACTC93430.15607584922100728No Hit
TTCCCATGTACTCTGCGTTGATACC89750.14992836848534094No Hit
GTACATGGGTAAGCAGTGGTATCAA83430.13937073852626178No Hit
GGTAAGCAGTGGTATCAACGCAGAG78850.13171979782806834No Hit
CCCCATGTACTCTGCGTTGATACCA77750.12988223565164633No Hit
TATCAACGCAGAGTACTTTTTTTTT74530.12450319000793827No Hit
GGTATCAACGCAGAGTACTTTTTTT68020.11362816294565893No Hit
ATTCCATTCCATTCCATTCCATTCC60230.10061488171445218No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA750.00261464110.1495052
ACGTCGC856.6294655E-410.0537516
CCTAGTA2700.09.515162
TACGACA1304.1602707E-69.5149214
TAATGCG800.00446050249.5149215
TTAGTAC2151.03682396E-109.2936433
ACGGGTC2054.110916E-109.282855
TAAGACG1054.426223E-49.06183054
TACGTCG1054.426223E-49.06183055
TTAGACT3050.09.0469754
TCGTGCG850.00747793778.9356229
TTAGGAC3850.08.8970693
CGTACCC1304.2934895E-58.7830053
CTTAGAC3850.08.6499283
ATTAACG1554.1243347E-68.5941223
GTGCACG2553.8198777E-118.5863281
TATACTG6800.08.5354445
TGCTACG1451.657486E-58.5308332
CCTATAC4950.08.4577073
GTCTTAG3300.08.3657291
Produced by FastQC (version 0.11.5)