FastQCFastQC Report
Wed 25 May 2016
SRR1294687_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294687_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5164834
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1417182.743902320965204No Hit
CCCATGTACTCTGCGTTGATACCAC1396162.7032040139140965No Hit
GAGTACATGGGAAGCAGTGGTATCA621361.2030589947324541No Hit
CATGTACTCTGCGTTGATACCACTG489940.9486074479838075No Hit
GCGTTGATACCACTGCTTCCCATGT462290.8950723295269509No Hit
GTATCAACGCAGAGTACATGGGAAG386510.7483493177128249No Hit
ACGCAGAGTACATGGGAAGCAGTGG361680.7002742004873729No Hit
GCTTCCCATGTACTCTGCGTTGATA314980.6098550311587942No Hit
ACTCTGCGTTGATACCACTGCTTCC307410.5951982193425772No Hit
TATCAACGCAGAGTACATGGGAAGC306520.5934750274645807No Hit
GGTATCAACGCAGAGTACATGGGAA249030.4821645768286067No Hit
GTGGTATCAACGCAGAGTACATGGG199640.38653710845305No Hit
CAGTGGTATCAACGCAGAGTACATG194980.3775145532266865No Hit
ATACCACTGCTTCCCATGTACTCTG169960.329071563577842No Hit
GTACTCTGCGTTGATACCACTGCTT160230.3102326231588469No Hit
GCAGTGGTATCAACGCAGAGTACAT154120.2984026204908038No Hit
CTGCTTCCCATGTACTCTGCGTTGA140570.2721675081909699No Hit
CATGGGAAGCAGTGGTATCAACGCA138930.2689921883258978No Hit
GTACATGGTAAGCAGTGGTATCAAC138520.2681983583596298No Hit
GGGAAGCAGTGGTATCAACGCAGAG137540.2663009111231842No Hit
ACATGGGAAGCAGTGGTATCAACGC132830.25718154736434895No Hit
TACCACTGCTTCCCATGTACTCTGC128930.24963048183155545No Hit
GATACCACTGCTTCCCATGTACTCT125790.243550905992332No Hit
CTTCCCATGTACTCTGCGTTGATAC118960.23032686045669618No Hit
CCATGTACTCTGCGTTGATACCACT117890.2282551578618016No Hit
GCAGAGTACATGGGAAGCAGTGGTA113450.21965856017831356No Hit
GTTGATACCACTGCTTCCCATGTAC107290.20773174897779872No Hit
AAGCAGTGGTATCAACGCAGAGTAC105910.20505983348157947No Hit
CCACTGCTTCCCATGTACTCTGCGT103790.20095515170477887No Hit
ACCATGTACTCTGCGTTGATACCAC102100.19768302330723506No Hit
ATGGGAAGCAGTGGTATCAACGCAG90020.17429408186206952No Hit
TTCCCATGTACTCTGCGTTGATACC87670.1697440808358991No Hit
TGATACCACTGCTTCCCATGTACTC85420.16538769687467206No Hit
GTACATGGGTAAGCAGTGGTATCAA77020.14912386341942452No Hit
ATCAACGCAGAGTACATGGGAAGCA70100.13572556252533963No Hit
GGTAAGCAGTGGTATCAACGCAGAG64710.12528960272488912No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA63020.12201747432734526No Hit
CCCCATGTACTCTGCGTTGATACCA61480.11903577152721656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAC1750.012.51332119
CGCAAAA3800.012.2493822
ATACCGT658.054573E-411.6878696
GCGACGT658.059153E-411.68707758
GATACGA1408.289135E-910.86385518
ACGCAAA4750.010.2012631
GCGTAGG750.00264663210.1345881
AACCGTC856.6250825E-410.054427
CGCCCTA1106.798291E-59.50568916
GTACAAA11200.09.5011771
TAGGCCG1002.7630467E-49.4981415
CCTACAC3650.09.3690273
GTCCTAA2650.09.3219091
ATACGAC1752.5802365E-89.24897719
GAACAAA18600.09.1436051
GTATCGA2201.6552804E-109.07378818
GTCTTAA4200.09.0487391
GTACTGG7250.09.04251
ATAACGC950.00182643328.9991983
CTAGACA4550.08.9767884
Produced by FastQC (version 0.11.5)