##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294687_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5164834
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.171985004745554	33.0	33.0	33.0	27.0	33.0
2	31.0973979028174	33.0	33.0	33.0	27.0	33.0
3	31.168419546494622	33.0	33.0	33.0	27.0	33.0
4	34.58340500391687	37.0	37.0	37.0	27.0	37.0
5	34.88908898136901	37.0	37.0	37.0	33.0	37.0
6	35.01992203428029	37.0	37.0	37.0	33.0	37.0
7	35.00080602784136	37.0	37.0	37.0	33.0	37.0
8	34.94467876411904	37.0	37.0	37.0	33.0	37.0
9	34.623031253279386	37.0	37.0	37.0	27.0	37.0
10	34.71806373641437	37.0	37.0	37.0	27.0	37.0
11	34.990238408436745	37.0	37.0	37.0	33.0	37.0
12	34.92660964515026	37.0	37.0	37.0	33.0	37.0
13	34.81354483028883	37.0	37.0	37.0	33.0	37.0
14	36.02312600947097	40.0	37.0	40.0	27.0	40.0
15	36.277677269008066	40.0	37.0	40.0	27.0	40.0
16	36.25201952279589	40.0	37.0	40.0	27.0	40.0
17	36.248012036785695	40.0	37.0	40.0	27.0	40.0
18	36.229989385912496	40.0	37.0	40.0	27.0	40.0
19	36.238422570793176	40.0	37.0	40.0	27.0	40.0
20	36.16593679487085	40.0	37.0	40.0	27.0	40.0
21	36.08579423849827	40.0	37.0	40.0	27.0	40.0
22	35.99914769768012	40.0	37.0	40.0	27.0	40.0
23	35.621062554963046	37.0	37.0	40.0	27.0	40.0
24	35.962515348992824	37.0	37.0	40.0	27.0	40.0
25	34.84119896205764	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.4710083832371694
1101	2	0.6205900733322274
1101	3	0.46636095696423396
1101	4	0.5976855776355663
1101	5	0.5166684928290763
1101	6	0.3526136376861757
1101	7	0.3897183014649812
1101	8	0.4798094315237975
1101	9	0.7954985996163373
1101	10	0.8103944599448738
1101	11	0.5531768184219317
1101	12	0.4929108702069982
1101	13	0.5218653397061246
1101	14	1.284447673408195
1101	15	0.9823963813498011
1101	16	0.9227968383332623
1101	17	1.011019253359251
1101	18	0.8919315288252037
1101	19	0.918315218717602
1101	20	1.0254920853928056
1101	21	0.9338152321422939
1101	22	0.9581337727388473
1101	23	1.3355600386172597
1101	24	1.123748906013141
1101	25	0.9911261061937111
1102	1	0.3737530370738611
1102	2	0.5125997303390868
1102	3	0.3860700836423234
1102	4	0.34417005398346134
1102	5	0.2509577187128258
1102	6	0.3033651746265207
1102	7	0.2957070345997508
1102	8	0.39154183609799986
1102	9	0.6110329826809675
1102	10	0.6361183255468106
1102	11	0.41528019307672537
1102	12	0.4929122812353839
1102	13	0.5079954485978604
1102	14	1.0521846128490324
1102	15	0.8900927624763568
1102	16	0.6331567195168191
1102	17	0.832750730226941
1102	18	0.8068004530396564
1102	19	0.7633921424485166
1102	20	0.9177088420729618
1102	21	1.013610173637666
1102	22	1.0147471431366242
1102	23	1.28763615613299
1102	24	1.0451330305577216
1102	25	0.9443764672473023
1103	1	0.30943544776748766
1103	2	0.477593546700529
1103	3	0.2884774384952813
1103	4	0.11732291097979441
1103	5	0.24495034483625489
1103	6	0.2331690047432673
1103	7	0.16012650156238095
1103	8	0.40021553104208607
1103	9	0.5789700423578026
1103	10	0.535848928950692
1103	11	0.39433891442449465
1103	12	0.35885368987296573
1103	13	0.32611878174181186
1103	14	1.0440254249616956
1103	15	0.7694025194426857
1103	16	0.5134785215052986
1103	17	0.5104602492894088
1103	18	0.6507337916471982
1103	19	0.7556169503730459
1103	20	0.7963547184005719
1103	21	0.8694124274870987
1103	22	0.7704543893575959
1103	23	1.1764492178035582
1103	24	0.8671264033912891
1103	25	0.7458131707923457
1104	1	0.3274724336750907
1104	2	0.48679905148767944
1104	3	0.2945705940391363
1104	4	0.2773176853338768
1104	5	0.24228634076708033
1104	6	0.21916538987310474
1104	7	0.1838744861785031
1104	8	0.3803190781891601
1104	9	0.5581536566705623
1104	10	0.572236997612741
1104	11	0.38873182630337766
1104	12	0.3915363345520788
1104	13	0.4279295483623784
1104	14	0.8629328368679978
1104	15	0.6372804882916938
1104	16	0.650811157314493
1104	17	0.6927932306731961
1104	18	0.5149382056028884
1104	19	0.5945453478961866
1104	20	0.7096428049998309
1104	21	0.8038973210792264
1104	22	0.8788279764875355
1104	23	1.2490826163168052
1104	24	0.9454037785394078
1104	25	0.6862929923499479
1105	1	0.22594319734632862
1105	2	0.3334267662972792
1105	3	0.39951992655430146
1105	4	0.43879560450464083
1105	5	0.40720665833722336
1105	6	0.3442189469490131
1105	7	0.29689374238398614
1105	8	0.4558607193632085
1105	9	-1.5120012811831671
1105	10	-3.368442788129755E-4
1105	11	0.25296093576698553
1105	12	0.367103273858568
1105	13	0.3650471715710708
1105	14	-1.3246754517444401
1105	15	-0.05322989668194822
1105	16	0.0791807291247082
1105	17	0.29610085950793064
1105	18	0.30671030065939675
1105	19	0.3999423563769824
1105	20	0.5247461913049847
1105	21	0.6712822772702509
1105	22	0.7649727049432116
1105	23	1.181100656299357
1105	24	0.8665012908362186
1105	25	0.4787267977434482
1106	1	0.06234308195951854
1106	2	0.046807886155413314
1106	3	0.15395414025060816
1106	4	-1.1211899503791827
1106	5	-0.2483807370432558
1106	6	-0.048671705296200685
1106	7	0.06385710204880013
1106	8	0.20618365008105854
1106	9	0.4682195576548409
1106	10	0.47545745713290444
1106	11	0.27923345122123777
1106	12	0.14559310962322058
1106	13	0.18653444930454555
1106	14	0.5640272720171566
1106	15	0.35552615748422056
1106	16	0.20194685502591625
1106	17	0.08961043508972466
1106	18	0.0994254450779124
1106	19	0.170612910791192
1106	20	0.3778149162723565
1106	21	0.4704054413065393
1106	22	0.4842649712644089
1106	23	0.7347394510462806
1106	24	0.4296835392148708
1106	25	0.3616231305958877
1107	1	0.36924664650432604
1107	2	0.3485298074140566
1107	3	0.3850576281294984
1107	4	0.5949377184816953
1107	5	0.4701256164118277
1107	6	0.24254008144287553
1107	7	0.3567278823591238
1107	8	0.38071095448366776
1107	9	0.5790089093976007
1107	10	0.5746926891133342
1107	11	0.26956646166119214
1107	12	0.2751030331164017
1107	13	0.39510538243639814
1107	14	0.8351494270758195
1107	15	0.6288788283988325
1107	16	0.6510900869074945
1107	17	0.6175966660434398
1107	18	0.6613934477146941
1107	19	0.5565535506403947
1107	20	0.7489406034463997
1107	21	0.7081680173133051
1107	22	0.6422014794391586
1107	23	0.9193881371443808
1107	24	0.8427710173055019
1107	25	0.7478653910856963
1108	1	0.5253715394592966
1108	2	0.4831166938678315
1108	3	0.5679396148440397
1108	4	0.7412484930593308
1108	5	0.6286496114676865
1108	6	0.42799109163375704
1108	7	0.46078968238374074
1108	8	0.5320205690670008
1108	9	0.3020047695695709
1108	10	0.6466680414140313
1108	11	0.4388003108462115
1108	12	0.5475508114816918
1108	13	0.46463119229527194
1108	14	-0.9122745719309364
1108	15	0.13122723261543712
1108	16	0.4453207764172262
1108	17	0.5825159240110835
1108	18	0.6192809902114931
1108	19	0.6312035382659147
1108	20	0.657511819551388
1108	21	0.7203298817409234
1108	22	0.9120852898085801
1108	23	1.0260032981945315
1108	24	1.0039826202398245
1108	25	0.8442170110838845
1109	1	0.43543443713911856
1109	2	0.35532217368215413
1109	3	0.44635910586501026
1109	4	0.5821560748774743
1109	5	0.588682677284524
1109	6	0.32216289241654295
1109	7	0.3108954244615205
1109	8	0.39805991968761845
1109	9	0.5494178847763393
1109	10	0.5637838858895208
1109	11	0.3187030762122234
1109	12	0.39647874329072863
1109	13	0.28902298467742327
1109	14	0.750669287033169
1109	15	0.46131168938595124
1109	16	0.5053077924640306
1109	17	0.4989331995525319
1109	18	0.5453663256161931
1109	19	0.4132330773310642
1109	20	0.6621236561835531
1109	21	0.6400888343911646
1109	22	0.752548560243028
1109	23	1.1471045056666824
1109	24	0.8790142820892584
1109	25	0.7607009423587101
1110	1	0.3760752475779654
1110	2	0.20119665146623333
1110	3	0.3193085600009553
1110	4	0.5197301154577971
1110	5	0.42949796073589397
1110	6	0.3189488997224288
1110	7	0.269438017443548
1110	8	0.3285215209128083
1110	9	0.46741732206574227
1110	10	0.49127818069418083
1110	11	0.2597423540141577
1110	12	0.17027314283880912
1110	13	0.17021875843970946
1110	14	0.6277589359953168
1110	15	0.3813750514529417
1110	16	0.38986368602190424
1110	17	0.38901796291384727
1110	18	0.4959701934524219
1110	19	0.4088294388042186
1110	20	0.5289227985656098
1110	21	0.5774607047225189
1110	22	0.7023175663429413
1110	23	0.8553317536755216
1110	24	0.7660125231584161
1110	25	0.6172202579158466
1111	1	0.20849053969207532
1111	2	0.18804955675773272
1111	3	0.3423744796788135
1111	4	0.554829538135138
1111	5	0.3624401334352285
1111	6	0.2783072551194934
1111	7	0.18401831943891267
1111	8	0.32587304948691553
1111	9	0.35234616933207263
1111	10	0.27966825813655305
1111	11	0.20878491271023591
1111	12	0.22344524013339395
1111	13	0.1820649039626545
1111	14	-1.8446732172974407
1111	15	-0.5586850293501016
1111	16	-0.04075653085683939
1111	17	0.15681219699354187
1111	18	0.17326597980393643
1111	19	0.21162556059109505
1111	20	0.3264206139711874
1111	21	0.44876506571456787
1111	22	0.1355742527472188
1111	23	-1.9308107868302216
1111	24	-0.3865437317725693
1111	25	0.0950194906095021
1112	1	-0.1660187073192958
1112	2	-0.2751031692198147
1112	3	-0.05019668841303471
1112	4	0.13152339222031628
1112	5	-0.7339011678218412
1112	6	-0.9107147242788542
1112	7	-1.0096398822203057
1112	8	-0.8309746562787907
1112	9	-0.7399769834898464
1112	10	-1.0128531795287756
1112	11	-0.9837268396802159
1112	12	-0.8735918542488506
1112	13	-0.9438559932296045
1112	14	-0.6754326605714098
1112	15	-0.9207864549933404
1112	16	-0.9241654509646793
1112	17	-0.9301497164418251
1112	18	-1.182755557953591
1112	19	-0.9209309353983102
1112	20	-0.9862272274214519
1112	21	-1.9116197669519792
1112	22	-1.7504090123218816
1112	23	-1.452583232385841
1112	24	-1.6231833940842861
1112	25	-2.1355727748397015
1113	1	0.07317045325351401
1113	2	-0.22839457219675197
1113	3	-0.15002211213782957
1113	4	0.16358657522689413
1113	5	0.06945196886462668
1113	6	-0.11309177256785574
1113	7	-0.08314199660292587
1113	8	0.010879654128977734
1113	9	0.18678392754779338
1113	10	-0.059297075029277835
1113	11	-0.12384196092740751
1113	12	-0.09399207150440958
1113	13	-0.22432293407390347
1113	14	-0.03763481208174113
1113	15	-0.20970295460166
1113	16	-0.13686316051773417
1113	17	-0.11831672850821207
1113	18	0.0024889449009748432
1113	19	-0.18990410688720516
1113	20	-0.6546336065196527
1113	21	-0.5908124775912285
1113	22	-0.149411666041523
1113	23	-0.3226509640887869
1113	24	-0.08088155618496273
1113	25	-1.1994747475467094
1114	1	-0.9893122469204236
1114	2	-0.6940527190092816
1114	3	-0.6343599622614917
1114	4	-1.624990429970417
1114	5	-0.9025081150565128
1114	6	-0.7025103731130216
1114	7	-0.6688610583303358
1114	8	-0.5129011485113537
1114	9	-0.5245991179799034
1114	10	-0.5492696442786169
1114	11	-0.5850793665319927
1114	12	-0.6025454472344194
1114	13	-0.6915904427183222
1114	14	-0.6015285108850321
1114	15	-1.4603965749058503
1114	16	-1.0743831453256192
1114	17	-0.9089068191678393
1114	18	-0.9335043429699255
1114	19	-1.060139667665183
1114	20	-2.125628724246347
1114	21	-6.820005333001053
1114	22	-7.823722557320423
1114	23	-7.864742529128893
1114	24	-5.981103522818611
1114	25	-7.332611198485662
1115	1	-1.1547637283514618
1115	2	-1.0893267331656844
1115	3	-0.8383487427367982
1115	4	-0.7211489791985741
1115	5	-0.8093355514230751
1115	6	-0.8767770614400519
1115	7	-1.0317549544081857
1115	8	-0.8599739561534818
1115	9	-2.82387398150453
1115	10	-1.581544670937859
1115	11	-1.2679805124688315
1115	12	-1.1184113252409844
1115	13	-1.5232647720523858
1115	14	-1.2053861927268557
1115	15	-2.172264924762601
1115	16	-1.4879442170774055
1115	17	-3.1706879110423998
1115	18	-2.9036753695241586
1115	19	-2.17975208460134
1115	20	-1.8461340176548262
1115	21	-1.3714884090209267
1115	22	-0.9404546625018497
1115	23	-0.3922258530042839
1115	24	-1.4187623638537303
1115	25	-1.0697988100511822
1116	1	-1.4399783763051062
1116	2	-0.6738096685637984
1116	3	-0.5916697623076921
1116	4	-0.17879883334165214
1116	5	-0.2170215977539982
1116	6	-0.2431842344826194
1116	7	-0.660065542701453
1116	8	-1.5820418281850692
1116	9	-0.6589191185179004
1116	10	-2.3503739545014426
1116	11	-0.9543690416811472
1116	12	-0.6257395706136535
1116	13	-0.6263123179309176
1116	14	-0.9379323615180013
1116	15	-0.9810764521764312
1116	16	-0.572534151945753
1116	17	-0.47612949883231437
1116	18	-0.9934439485840514
1116	19	-0.5893356114864332
1116	20	-0.8687121043696848
1116	21	-0.38965331722989305
1116	22	-0.008683861669112503
1116	23	0.16003635994032805
1116	24	0.0712481906863971
1116	25	0.10136946716876594
1201	1	0.336295392513307
1201	2	0.42294170921651286
1201	3	0.43218503683332443
1201	4	0.5458410988405902
1201	5	0.51348926304793
1201	6	0.37592465897578364
1201	7	0.33386904703407794
1201	8	0.4360503455592948
1201	9	0.7408818682929308
1201	10	0.6957305212513063
1201	11	0.5173034767404587
1201	12	0.50806527942472
1201	13	0.5653100014310795
1201	14	1.2086403554835812
1201	15	0.9200222994931195
1201	16	0.8681928006040636
1201	17	0.8177345700331315
1201	18	0.7041080066209062
1201	19	0.7217548414056694
1201	20	0.912275536444703
1201	21	0.9070919096149908
1201	22	1.0567693286980102
1201	23	0.8101120845224159
1201	24	0.8959422332217883
1201	25	0.7816911531838358
1202	1	-0.22537029133324893
1202	2	0.006560275691182227
1202	3	-0.02337528752750373
1202	4	-0.06930503547481948
1202	5	-0.02253569849992232
1202	6	-0.08798715555633407
1202	7	-0.10192286714259069
1202	8	0.041368442618292534
1202	9	0.12271067476784481
1202	10	0.3704231269733924
1202	11	0.12344710056424191
1202	12	0.08538686984226018
1202	13	0.19972437135399446
1202	14	0.36442047862734483
1202	15	0.3316797020944051
1202	16	0.3427271113232635
1202	17	0.1959301600576424
1202	18	0.16477019591535935
1202	19	0.17859291381822828
1202	20	0.36691776242658136
1202	21	0.4347797739994377
1202	22	0.30587002899156346
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2211	3	0.16386894625584603
2211	4	0.29097985549975647
2211	5	0.21892670367967781
2211	6	0.12453967134631228
2211	7	0.08716655904594717
2211	8	0.25326048603860585
2211	9	0.41254392294129616
2211	10	0.3349686124301243
2211	11	0.11422538499333967
2211	12	0.22966390241334267
2211	13	0.11061622076054078
2211	14	0.36793088144207076
2211	15	0.23030414299705626
2211	16	0.14145214397558448
2211	17	0.2933593931155585
2211	18	0.1597078214915726
2211	19	0.2232203250853999
2211	20	0.268004731825485
2211	21	0.2989872970252989
2211	22	0.3277207325568483
2211	23	0.496254702984416
2211	24	0.4429895917699582
2211	25	0.4830830669482822
2212	1	-0.41189452557705053
2212	2	-0.4300184375363294
2212	3	-0.37606365389009255
2212	4	-0.6865566696794474
2212	5	-0.3995072144337044
2212	6	-0.7604449293973232
2212	7	-0.6868506231295086
2212	8	-0.4412211690441623
2212	9	-0.412755176604783
2212	10	-0.6764444633736701
2212	11	-0.6765786549359234
2212	12	-0.7393650232364664
2212	13	-0.7599101973419593
2212	14	-0.6730727032552934
2212	15	-0.7618921405813737
2212	16	-0.6584894650617414
2212	17	-0.6537353482161095
2212	18	-0.5799926655108152
2212	19	-0.7405170737096469
2212	20	-0.8058034034500494
2212	21	-0.671441415950838
2212	22	-0.6619252078568039
2212	23	-0.12349377341040224
2212	24	-0.39052000248176455
2212	25	-1.0822807196294875
2213	1	-0.09057492913807152
2213	2	-1.2549206334267424
2213	3	-0.2643550660080649
2213	4	0.13710508419380574
2213	5	0.1508460999666994
2213	6	0.03340103066529565
2213	7	0.06684935267831094
2213	8	0.18928714384101397
2213	9	0.25484342224799406
2213	10	-0.3042592323726083
2213	11	-0.01621123366548005
2213	12	0.07208152075175178
2213	13	-0.29087799032085826
2213	14	0.05827352457794177
2213	15	-0.05277547228190116
2213	16	0.04335218268008845
2213	17	0.15433615782008303
2213	18	0.2269919346084066
2213	19	0.1902070551504309
2213	20	0.2843913221971732
2213	21	-0.4167761235067715
2213	22	-0.821634813124362
2213	23	-0.09134170597847913
2213	24	0.12194873976181952
2213	25	-0.39386940340052945
2214	1	-0.15164850409032482
2214	2	-0.4933902533919543
2214	3	-0.4641133500364134
2214	4	-0.49534504652363154
2214	5	-0.28920108124248856
2214	6	-0.21949216886643086
2214	7	-0.4404962396681853
2214	8	-0.06689779660351292
2214	9	-0.02497914990571104
2214	10	-1.9682881438418747
2214	11	-0.8102607664401376
2214	12	-0.45577924166590833
2214	13	-0.36663694528163404
2214	14	-0.7570786105782759
2214	15	-0.6765521071847118
2214	16	-0.6626342198661845
2214	17	-0.5723668098320616
2214	18	-0.571049858359828
2214	19	-1.4574839232600638
2214	20	-2.543750878316253
2214	21	-1.851392793085168
2214	22	-2.0805339742373903
2214	23	-2.6523236835338437
2214	24	-1.7730352279748303
2214	25	-1.987601161802857
2215	1	-0.2061379835675119
2215	2	-0.33449803080050344
2215	3	-0.83907418003988
2215	4	-0.33471426491725254
2215	5	-0.20368179671555708
2215	6	-0.25218772955818025
2215	7	-0.25396219482680493
2215	8	-0.27315562030329943
2215	9	-0.1814560529418685
2215	10	-0.28937307112003197
2215	11	-0.5570345447231091
2215	12	-0.4516807761892494
2215	13	-0.2945068360882317
2215	14	-0.17675799465263253
2215	15	-0.6512429671687272
2215	16	-0.36405838326278683
2215	17	-0.26246624377846217
2215	18	-0.31277623533668475
2215	19	-2.185040237050856
2215	20	-2.386414777579432
2215	21	-3.361840097195966
2215	22	-2.3365520994637166
2215	23	-3.617699061221437
2215	24	-2.5669781427616982
2215	25	-1.7937594884528352
2216	1	-1.3639787553242648
2216	2	-0.9424028290621536
2216	3	-0.7290389768689778
2216	4	-0.7870594325619749
2216	5	-1.7735539312841269
2216	6	-1.5371804252279375
2216	7	-0.9769694047160513
2216	8	-5.148524648132469
2216	9	-4.092983409362404
2216	10	-1.975839220142852
2216	11	-0.8881669538238341
2216	12	-1.0679311311577564
2216	13	-1.7807353762006315
2216	14	-3.334534308981233
2216	15	-1.918319177503598
2216	16	-2.8515700752246502
2216	17	-2.5889480277109413
2216	18	-2.4835161696755748
2216	19	-1.482574885272264
2216	20	-0.8081879029310954
2216	21	-0.6140319865250206
2216	22	-0.5412851722007375
2216	23	-0.8757439457532001
2216	24	-0.5209524248272572
2216	25	-0.09891816127583297
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	6.0
7	85.0
8	391.0
9	1000.0
10	2036.0
11	3867.0
12	6639.0
13	10062.0
14	13627.0
15	18941.0
16	23596.0
17	25817.0
18	27406.0
19	28000.0
20	28933.0
21	31656.0
22	33625.0
23	35506.0
24	38260.0
25	42010.0
26	44963.0
27	50047.0
28	57077.0
29	67855.0
30	81883.0
31	100605.0
32	135393.0
33	195016.0
34	284409.0
35	453701.0
36	790540.0
37	2531882.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.219128575495766	19.470587437805886	12.271254456962351	24.039029529736
2	13.493332662016508	21.81950743418836	38.12528321178626	26.56187669200888
3	20.335570326826474	27.77437254859811	27.589057071705604	24.301000052869814
4	13.151492451174137	18.41982844035347	33.93371333751142	34.49496577096098
5	13.035399818843842	38.682940121440865	34.26113406388417	14.020525995831123
6	33.18226670807848	33.62319020182063	17.833168228258607	15.361374861842295
7	25.522127478393173	32.80359487419083	22.970123842935692	18.70415380448031
8	26.20143697678795	36.140094006813904	20.996210451965368	16.662258564432772
9	26.23740349439641	17.438369431408365	20.594354860257834	35.72987221393739
10	16.548026271078637	30.73091196563175	31.58003061899985	21.141031144289762
11	30.55180739454243	27.099755941956587	20.223360062577992	22.125076600922984
12	23.20004151210957	29.305012935746454	29.233663997422532	18.26128155472144
13	27.967399297612978	22.628649939957487	24.12365233507478	25.280298427354758
14	21.350425698879782	24.923980614161128	25.99697452041231	27.728619166546785
15	29.066395958586	26.07586035041155	21.828704595615605	23.029039095386842
16	20.727570412663205	29.210818991088733	28.815771538150553	21.245839058097516
17	24.284879773059025	29.49860891968174	27.35319499269172	18.863316314567513
18	24.7893342048914	27.032737835346392	28.09130913197812	20.08661882778409
19	22.8193160139583	29.715751567384917	28.721891770331275	18.743040648325508
20	23.877003240578226	27.353620717395906	26.905589527919567	21.863786514106305
21	21.17108152734498	29.23531792945847	28.516811053367626	21.076789489828922
22	22.02978019069336	28.517698797809466	24.266065409923293	25.186455601573883
23	19.628471225771346	29.61338212021407	27.28568364189427	23.472463012120308
24	21.652587635506922	32.16629269088884	25.077113747303127	21.104005926301117
25	22.864257438385902	26.202657274746656	23.541210037730956	27.39187524913649
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8518.0
1	8518.0
2	10592.5
3	12667.0
4	12667.0
5	12667.0
6	15850.5
7	19034.0
8	19034.0
9	19034.0
10	20142.0
11	21250.0
12	21250.0
13	21250.0
14	27675.0
15	34100.0
16	34100.0
17	34100.0
18	49810.5
19	65521.0
20	65521.0
21	65521.0
22	92427.0
23	119333.0
24	119333.0
25	119333.0
26	157405.0
27	195477.0
28	195477.0
29	195477.0
30	239707.5
31	283938.0
32	283938.0
33	283938.0
34	329327.5
35	374717.0
36	374717.0
37	374717.0
38	420382.0
39	466047.0
40	466047.0
41	466047.0
42	513292.0
43	560537.0
44	560537.0
45	560537.0
46	822519.0
47	1084501.0
48	1084501.0
49	1084501.0
50	951676.0
51	818851.0
52	818851.0
53	818851.0
54	628263.5
55	437676.0
56	437676.0
57	437676.0
58	355460.0
59	273244.0
60	273244.0
61	273244.0
62	230004.0
63	186764.0
64	186764.0
65	186764.0
66	147489.0
67	108214.0
68	108214.0
69	108214.0
70	81792.0
71	55370.0
72	55370.0
73	55370.0
74	40044.0
75	24718.0
76	24718.0
77	24718.0
78	17120.5
79	9523.0
80	9523.0
81	9523.0
82	6469.5
83	3416.0
84	3416.0
85	3416.0
86	2216.5
87	1017.0
88	1017.0
89	1017.0
90	656.0
91	295.0
92	295.0
93	295.0
94	184.0
95	73.0
96	73.0
97	73.0
98	53.0
99	33.0
100	33.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030281708957151383
2	0.016534897346168337
3	0.023369579738671174
4	0.026757878375181083
5	0.025809154756958306
6	0.008635321096476672
7	0.0028461708546683204
8	0.0019748940624229163
9	0.00209106430138897
10	0.0012972343351209353
11	0.0015295748130530428
12	0.0014714896935700162
13	0.00309787303909477
14	6.389363143132964E-4
15	0.002071702594894628
16	0.0026719154962192397
17	0.0027687240286909513
18	0.0026525537897248972
19	4.0659583638118864E-4
20	2.9042559741513475E-4
21	0.08269384843733603
22	0.07915065614887139
23	0.080486613896981
24	0.0011810640961548813
25	0.2330374993659041
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5164834.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.26002526716725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.15394507019775	42.93367245166005
2	11.41528915767876	11.931265996246246
3	3.052161559684696	4.785181206857964
4	1.2334225366596578	2.578347717237089
5	0.6079497176942583	1.5885733803934567
6	0.34292107119413634	1.0752638307149776
7	0.2290492462472535	0.8379083577414956
8	0.15286139603258053	0.6390832335229695
9	0.11053557751991125	0.5198932866700338
>10	0.6187260774301829	5.942028268153651
>50	0.04880728125158645	1.7687362609104293
>100	0.028027448505659393	2.6940048824494602
>500	0.002340355321679125	0.881478565504896
>1k	0.0025290933999624777	3.0762187206822253
>5k	3.019813014880536E-4	1.1825115749171116
>10k+	0.0011324298805802009	17.56583226633803
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	141718	2.743902320965204	No Hit
CCCATGTACTCTGCGTTGATACCAC	139616	2.7032040139140965	No Hit
GAGTACATGGGAAGCAGTGGTATCA	62136	1.2030589947324541	No Hit
CATGTACTCTGCGTTGATACCACTG	48994	0.9486074479838075	No Hit
GCGTTGATACCACTGCTTCCCATGT	46229	0.8950723295269509	No Hit
GTATCAACGCAGAGTACATGGGAAG	38651	0.7483493177128249	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	36168	0.7002742004873729	No Hit
GCTTCCCATGTACTCTGCGTTGATA	31498	0.6098550311587942	No Hit
ACTCTGCGTTGATACCACTGCTTCC	30741	0.5951982193425772	No Hit
TATCAACGCAGAGTACATGGGAAGC	30652	0.5934750274645807	No Hit
GGTATCAACGCAGAGTACATGGGAA	24903	0.4821645768286067	No Hit
GTGGTATCAACGCAGAGTACATGGG	19964	0.38653710845305	No Hit
CAGTGGTATCAACGCAGAGTACATG	19498	0.3775145532266865	No Hit
ATACCACTGCTTCCCATGTACTCTG	16996	0.329071563577842	No Hit
GTACTCTGCGTTGATACCACTGCTT	16023	0.3102326231588469	No Hit
GCAGTGGTATCAACGCAGAGTACAT	15412	0.2984026204908038	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	14057	0.2721675081909699	No Hit
CATGGGAAGCAGTGGTATCAACGCA	13893	0.2689921883258978	No Hit
GTACATGGTAAGCAGTGGTATCAAC	13852	0.2681983583596298	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	13754	0.2663009111231842	No Hit
ACATGGGAAGCAGTGGTATCAACGC	13283	0.25718154736434895	No Hit
TACCACTGCTTCCCATGTACTCTGC	12893	0.24963048183155545	No Hit
GATACCACTGCTTCCCATGTACTCT	12579	0.243550905992332	No Hit
CTTCCCATGTACTCTGCGTTGATAC	11896	0.23032686045669618	No Hit
CCATGTACTCTGCGTTGATACCACT	11789	0.2282551578618016	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	11345	0.21965856017831356	No Hit
GTTGATACCACTGCTTCCCATGTAC	10729	0.20773174897779872	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	10591	0.20505983348157947	No Hit
CCACTGCTTCCCATGTACTCTGCGT	10379	0.20095515170477887	No Hit
ACCATGTACTCTGCGTTGATACCAC	10210	0.19768302330723506	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	9002	0.17429408186206952	No Hit
TTCCCATGTACTCTGCGTTGATACC	8767	0.1697440808358991	No Hit
TGATACCACTGCTTCCCATGTACTC	8542	0.16538769687467206	No Hit
GTACATGGGTAAGCAGTGGTATCAA	7702	0.14912386341942452	No Hit
ATCAACGCAGAGTACATGGGAAGCA	7010	0.13572556252533963	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	6471	0.12528960272488912	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	6302	0.12201747432734526	No Hit
CCCCATGTACTCTGCGTTGATACCA	6148	0.11903577152721656	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.9361706494342317E-5	0.0
4	0.0	0.0	0.0	3.8723412988684634E-5	0.0
5	0.0	0.0	0.0	3.8723412988684634E-5	0.0
6	0.0	0.0	0.0	3.8723412988684634E-5	0.0
7	0.0	0.0	0.0	5.8085119483026944E-5	0.0
8	0.0	0.0	0.0	5.8085119483026944E-5	0.0
9	0.0	0.0	0.0	5.8085119483026944E-5	0.0
10	0.0	0.0	0.0	9.680853247171158E-5	0.0
11	0.0	0.0	0.0	1.1617023896605389E-4	0.0
12	0.0	0.0	0.0	1.9361706494342316E-4	0.0
13	0.0	0.0	0.0	1.9361706494342316E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGAC	175	0.0	12.513321	19
CGCAAAA	380	0.0	12.249382	2
ATACCGT	65	8.054573E-4	11.687869	6
GCGACGT	65	8.059153E-4	11.6870775	8
GATACGA	140	8.289135E-9	10.863855	18
ACGCAAA	475	0.0	10.201263	1
GCGTAGG	75	0.002646632	10.134588	1
AACCGTC	85	6.6250825E-4	10.05442	7
CGCCCTA	110	6.798291E-5	9.505689	16
GTACAAA	1120	0.0	9.501177	1
TAGGCCG	100	2.7630467E-4	9.498141	5
CCTACAC	365	0.0	9.369027	3
GTCCTAA	265	0.0	9.321909	1
ATACGAC	175	2.5802365E-8	9.248977	19
GAACAAA	1860	0.0	9.143605	1
GTATCGA	220	1.6552804E-10	9.073788	18
GTCTTAA	420	0.0	9.048739	1
GTACTGG	725	0.0	9.0425	1
ATAACGC	95	0.0018264332	8.999198	3
CTAGACA	455	0.0	8.976788	4
>>END_MODULE