FastQCFastQC Report
Wed 25 May 2016
SRR1294687_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294687_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5164834
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1544422.9902606743992157No Hit
GTACATGGGAAGCAGTGGTATCAAC1353702.6209942081391193No Hit
GAGTACATGGGAAGCAGTGGTATCA674541.3060245498693666No Hit
CATGTACTCTGCGTTGATACCACTG569321.1023006741358967No Hit
GCGTTGATACCACTGCTTCCCATGT505450.9786374547565323No Hit
ACGCAGAGTACATGGGAAGCAGTGG431010.8345089116126482No Hit
GTATCAACGCAGAGTACATGGGAAG399030.7725901742437414No Hit
ACTCTGCGTTGATACCACTGCTTCC364860.7064312231525738No Hit
GCTTCCCATGTACTCTGCGTTGATA343780.6656167458625001No Hit
TATCAACGCAGAGTACATGGGAAGC340270.658820786882986No Hit
GGTATCAACGCAGAGTACATGGGAA268130.5191454362328005No Hit
CAGTGGTATCAACGCAGAGTACATG230360.4460162708036696No Hit
GTGGTATCAACGCAGAGTACATGGG206540.39989668593414623No Hit
ATACCACTGCTTCCCATGTACTCTG188690.3653360398417451No Hit
GCAGTGGTATCAACGCAGAGTACAT174290.3374551824898922No Hit
CATGGGAAGCAGTGGTATCAACGCA173600.33611922474178263No Hit
ACATGGGAAGCAGTGGTATCAACGC162560.3147439007720287No Hit
GGGAAGCAGTGGTATCAACGCAGAG161570.3128270918290888No Hit
GTACTCTGCGTTGATACCACTGCTT155400.3008809189220796No Hit
CTGCTTCCCATGTACTCTGCGTTGA153480.2971634712751659No Hit
TACCACTGCTTCCCATGTACTCTGC148420.28736644778902865No Hit
GATACCACTGCTTCCCATGTACTCT143680.2781889989107104No Hit
CCATGTACTCTGCGTTGATACCACT140410.27185772088706045No Hit
CTTCCCATGTACTCTGCGTTGATAC139720.2705217631389508No Hit
GTACATGGTAAGCAGTGGTATCAAC128690.24916580087569126No Hit
AAGCAGTGGTATCAACGCAGAGTAC123640.23938813909604842No Hit
GCAGAGTACATGGGAAGCAGTGGTA123540.23919452203110497No Hit
CCACTGCTTCCCATGTACTCTGCGT118840.2300945199787641No Hit
GTATCAACGCAGAGTACTTTTTTTT117550.22759685984099393No Hit
ACCATGTACTCTGCGTTGATACCAC116730.22600919990845786No Hit
GTTGATACCACTGCTTCCCATGTAC114340.22138175205631003No Hit
ATGGGAAGCAGTGGTATCAACGCAG108670.21040366447401793No Hit
TTCCCATGTACTCTGCGTTGATACC91660.17746940172714165No Hit
TATCAACGCAGAGTACTTTTTTTTT89270.17284195387499385No Hit
TGATACCACTGCTTCCCATGTACTC86790.16804025066439696No Hit
ATCAACGCAGAGTACATGGGAAGCA83110.16091514267447898No Hit
GGTATCAACGCAGAGTACTTTTTTT81200.1572170567340596No Hit
GTACATGGGTAAGCAGTGGTATCAA80820.1564813118872746No Hit
GGTAAGCAGTGGTATCAACGCAGAG75630.14643258621671093No Hit
CCCCATGTACTCTGCGTTGATACCA70520.13653875419810202No Hit
ATTCCATTCCATTCCATTCCATTCC60360.11686726039985022No Hit
ACGCAGAGTACTTTTTTTTTTTTTT56740.10985832264889829No Hit
GTACTTTTTTTTTTTTTTTTTTTTT55030.10654747083836576No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGC802.8860271E-511.8682910
TCGCGCG600.00590357711.077079
TACGCAG1053.409319E-610.8733255
TCGACCG1002.41636E-510.4440969
CTAGACA4350.010.2796664
TCTACGC1303.769528E-710.2460173
ATCGACC856.6322536E-410.0532378
ATCGCGC1054.1343876E-59.9468538
TATTCCG1401.0387412E-69.5141595
CGCAAAA1804.08545E-99.5141592
TAAACCG1002.7166816E-49.5141585
GGTCGGC1451.7221482E-69.16705311
TCTAGAC3750.09.1335933
CTTAGAC2850.09.0134133
TCTATAC4550.08.9914043
GTCCTAA3500.08.9748461
GTATTAG4350.08.971721
TAGACAG5100.08.9545035
TATACTG8500.08.8425725
AAAAGTA10550.08.8377972
Produced by FastQC (version 0.11.5)