FastQCFastQC Report
Wed 25 May 2016
SRR1294686_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294686_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4720264
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1530273.241916130114756No Hit
CCCATGTACTCTGCGTTGATACCAC1485003.146010477380079No Hit
GAGTACATGGGAAGCAGTGGTATCA682941.4468258555029974No Hit
GCGTTGATACCACTGCTTCCCATGT520011.101654483732266No Hit
CATGTACTCTGCGTTGATACCACTG519791.1011884081059873No Hit
GTATCAACGCAGAGTACATGGGAAG436420.9245669310021644No Hit
ACGCAGAGTACATGGGAAGCAGTGG397930.8430248816591615No Hit
GCTTCCCATGTACTCTGCGTTGATA379120.8031754156123471No Hit
ACTCTGCGTTGATACCACTGCTTCC324620.6877157718297112No Hit
TATCAACGCAGAGTACATGGGAAGC320460.678902705441899No Hit
GGTATCAACGCAGAGTACATGGGAA260030.5508802050054827No Hit
GTGGTATCAACGCAGAGTACATGGG218920.46378761865861734No Hit
CAGTGGTATCAACGCAGAGTACATG217890.4616055373174043No Hit
GCAGTGGTATCAACGCAGAGTACAT189410.4012699289700746No Hit
ATACCACTGCTTCCCATGTACTCTG179780.3808685276925189No Hit
CATGGGAAGCAGTGGTATCAACGCA156060.3306171010773974No Hit
GTACTCTGCGTTGATACCACTGCTT155330.32907057740838225No Hit
ACATGGGAAGCAGTGGTATCAACGC150430.318689802095815No Hit
GGGAAGCAGTGGTATCAACGCAGAG149520.31676194382348105No Hit
CTGCTTCCCATGTACTCTGCGTTGA147830.3131816356034324No Hit
GTACATGGTAAGCAGTGGTATCAAC143670.3043685692156201No Hit
TACCACTGCTTCCCATGTACTCTGC135540.28714495629905445No Hit
GATACCACTGCTTCCCATGTACTCT133740.28333161026586645No Hit
GCAGAGTACATGGGAAGCAGTGGTA133410.2826324968264487No Hit
CTTCCCATGTACTCTGCGTTGATAC129640.27464565541249386No Hit
CCATGTACTCTGCGTTGATACCACT121510.2574220424959282No Hit
AAGCAGTGGTATCAACGCAGAGTAC110240.23354625927702347No Hit
GTTGATACCACTGCTTCCCATGTAC110140.23333440671962413No Hit
CCACTGCTTCCCATGTACTCTGCGT107930.22865246520109894No Hit
ATGGGAAGCAGTGGTATCAACGCAG101450.2149244194816222No Hit
ACCATGTACTCTGCGTTGATACCAC100840.2136321188814863No Hit
TTCCCATGTACTCTGCGTTGATACC97530.20661979923156842No Hit
GTACATGGGTAAGCAGTGGTATCAA90670.19208671379397424No Hit
TGATACCACTGCTTCCCATGTACTC90630.1920019727710145No Hit
ATCAACGCAGAGTACATGGGAAGCA79610.16865582094560813No Hit
CCCCATGTACTCTGCGTTGATACCA71800.1521101362127203No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA70510.14937723822226892No Hit
GGTAAGCAGTGGTATCAACGCAGAG69850.14797901134343333No Hit
AAAAAGTACTCTGCGTTGATACCAC50130.10620168702428509No Hit
GCTTACCATGTACTCTGCGTTGATA49570.10501531270284883No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTCA400.005277501414.251511
TACGTCT658.045164E-411.6894594
CCGCTCC600.005894912411.0792249
GATACGA1106.0120565E-610.36946118
GTACAAA9350.010.1615051
TAGAACG856.6130457E-410.0563734
TATTCCG1651.0282747E-89.785395
GTCTGCG1752.5956979E-99.7678069
CGCAGAT3150.09.6471138
CGCCCTA800.0044978319.50523816
GTAGGAC2700.09.4981883
TGGACCG800.00452781569.4975845
CCGTGTC1304.2636148E-69.4964789
AACCGTG1002.768092E-49.4963787
GTCCTAG4000.09.2634811
AAGCGGT1951.6971171E-99.25297810
CGCATAG1752.588422E-89.24709119
GGTATCG1554.2317515E-79.19871517
GTGTAGG4300.09.05911
CCGCCCG1054.5124564E-49.0442659
Produced by FastQC (version 0.11.5)