##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294686_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4720264
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.96273810108926	33.0	33.0	33.0	27.0	33.0
2	30.99866893038186	33.0	33.0	33.0	27.0	33.0
3	31.02847361927214	33.0	33.0	33.0	27.0	33.0
4	34.42859954443226	37.0	37.0	37.0	27.0	37.0
5	34.744198417715616	37.0	37.0	37.0	27.0	37.0
6	34.8471945636939	37.0	37.0	37.0	33.0	37.0
7	34.87190504598895	37.0	37.0	37.0	33.0	37.0
8	34.795904635842405	37.0	37.0	37.0	27.0	37.0
9	34.516843125723476	37.0	37.0	37.0	27.0	37.0
10	34.614439785571314	37.0	37.0	37.0	27.0	37.0
11	34.855245596432745	37.0	37.0	37.0	33.0	37.0
12	34.801065999698324	37.0	37.0	37.0	27.0	37.0
13	34.65972157489497	37.0	37.0	37.0	27.0	37.0
14	35.850682504198915	40.0	37.0	40.0	27.0	40.0
15	36.084159275837116	40.0	37.0	40.0	27.0	40.0
16	36.06443580274323	37.0	37.0	40.0	27.0	40.0
17	36.066057534070126	40.0	37.0	40.0	27.0	40.0
18	36.04963408826286	40.0	37.0	40.0	27.0	40.0
19	36.07046957543053	40.0	37.0	40.0	27.0	40.0
20	35.992983231446374	40.0	37.0	40.0	27.0	40.0
21	35.926615968937334	37.0	37.0	40.0	27.0	40.0
22	35.83828785847571	37.0	37.0	40.0	27.0	40.0
23	35.492270135738174	37.0	37.0	40.0	27.0	40.0
24	35.81714730362539	37.0	37.0	40.0	27.0	40.0
25	34.65788756730556	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5359521565921064
1101	2	0.6590861380023192
1101	3	0.44007049472696735
1101	4	0.6168799139577601
1101	5	0.5618844746387666
1101	6	0.4282331093400984
1101	7	0.5641904600580006
1101	8	0.580544343226201
1101	9	0.754668232638771
1101	10	0.7845145088081793
1101	11	0.5676827287988289
1101	12	0.5822875579147606
1101	13	0.6047678653153952
1101	14	1.2686384530715102
1101	15	1.0709298970875878
1101	16	1.0338125408735905
1101	17	1.0358449629090032
1101	18	1.0578770950163872
1101	19	0.9462222222948
1101	20	1.1098178713691311
1101	21	1.0615469259311041
1101	22	1.149745471537976
1101	23	1.4919636731824966
1101	24	1.2126953617392076
1101	25	1.0872703061798745
1102	1	0.2897722842707964
1102	2	0.5395350490881299
1102	3	0.37290400456604544
1102	4	0.3491082768408802
1102	5	0.329073654494465
1102	6	0.24793114289728635
1102	7	0.32363233413940407
1102	8	0.41662819085538416
1102	9	0.6889823074824619
1102	10	0.6752921856128324
1102	11	0.44983137735363954
1102	12	0.5225276769935263
1102	13	0.48904585745842155
1102	14	1.1891310472815348
1102	15	0.9337527073467342
1102	16	0.7570449625436169
1102	17	0.8801388440803919
1102	18	0.8173167843954019
1102	19	0.8756309380557354
1102	20	0.9614031187293293
1102	21	1.011199211040065
1102	22	1.0595814386210662
1102	23	1.313450665893626
1102	24	1.07940284592879
1102	25	0.9761344177694795
1103	1	0.4241147089823585
1103	2	0.5698116163713962
1103	3	0.36402470173050006
1103	4	0.30860652730584803
1103	5	0.43212621845543
1103	6	0.30939450252240164
1103	7	0.3917270077479955
1103	8	0.5911034907257147
1103	9	0.6912911635050563
1103	10	0.732371872294145
1103	11	0.5772599871188646
1103	12	0.5472620855103258
1103	13	0.5510345297495007
1103	14	1.2479275359839335
1103	15	0.8472277250252631
1103	16	0.6534291357189517
1103	17	0.7214806996935721
1103	18	0.7814262544073642
1103	19	0.9112563577010562
1103	20	0.958306738134759
1103	21	1.0069523443081039
1103	22	0.8312757155380126
1103	23	1.3320887731943714
1103	24	0.9787431540086473
1103	25	0.9529566243392864
1104	1	0.3321808039328715
1104	2	0.4900657217209812
1104	3	0.3009942166221222
1104	4	0.3357388149757412
1104	5	0.2407791855728334
1104	6	0.143183112986911
1104	7	0.16921655929589008
1104	8	0.3835936880356172
1104	9	0.5684315046326915
1104	10	0.6423692926739335
1104	11	0.42318505747378765
1104	12	0.36893819269317873
1104	13	0.37495427427226247
1104	14	0.9079370965370757
1104	15	0.7183101863005916
1104	16	0.6313071309187137
1104	17	0.6554402514162874
1104	18	0.5954174699874031
1104	19	0.6833868272966725
1104	20	0.7076114156568991
1104	21	0.8192895255400003
1104	22	0.9206863117403117
1104	23	1.19834691049072
1104	24	0.970747931009754
1104	25	0.8315550050477327
1105	1	0.35959129469199524
1105	2	0.39957926116845854
1105	3	0.3386479557587556
1105	4	0.3217306595062226
1105	5	0.36886731083041724
1105	6	0.37558600408009113
1105	7	0.33612431552810307
1105	8	0.4372293807998986
1105	9	-1.5893763161352368
1105	10	-0.01958765798606521
1105	11	0.1434659050008804
1105	12	0.3065086279824598
1105	13	0.33095522849382775
1105	14	-1.5397185918181222
1105	15	-0.16914157149252418
1105	16	-9.535224753491889E-4
1105	17	0.288299789354312
1105	18	0.2979183764788331
1105	19	0.3104066740762832
1105	20	0.529511105401518
1105	21	0.6680476560964834
1105	22	0.5761878811184857
1105	23	0.9516369265855928
1105	24	0.7421188070929929
1105	25	0.49976723245961807
1106	1	0.12165441440579627
1106	2	0.09517815389700957
1106	3	0.1043932964281673
1106	4	-1.1867369302977266
1106	5	-0.22349744131103932
1106	6	-0.056319653555988225
1106	7	0.08522038940066778
1106	8	0.21145213844226163
1106	9	0.4371587743729961
1106	10	0.45996400729516296
1106	11	0.37990181047045013
1106	12	0.11732826397641105
1106	13	0.21494831170836903
1106	14	0.6956091943673428
1106	15	0.4039426604487062
1106	16	0.2519785001063255
1106	17	0.09894353829211155
1106	18	0.24148930763585952
1106	19	0.21036405113936496
1106	20	0.4625288131892944
1106	21	0.5242367493262563
1106	22	0.5972285436315374
1106	23	0.7758982028766397
1106	24	0.47998076966727865
1106	25	0.3830443582400562
1107	1	0.29879030810763396
1107	2	0.27111548475791025
1107	3	0.19661455633517022
1107	4	0.4200709084688796
1107	5	0.2574950183163338
1107	6	0.14756578182607427
1107	7	0.10090025595148688
1107	8	0.15303994025046563
1107	9	0.28544534238938724
1107	10	0.34700610500203055
1107	11	0.15497310338746217
1107	12	0.12440750308551429
1107	13	0.08870652474848129
1107	14	0.6106680702154108
1107	15	0.41666878355520254
1107	16	0.4500838219229877
1107	17	0.3878480590842983
1107	18	0.583081455875714
1107	19	0.3836769150672268
1107	20	0.5215196358132488
1107	21	0.5441713606935039
1107	22	0.44087797235028603
1107	23	0.7061906811250367
1107	24	0.6759365751502813
1107	25	0.48810264388846036
1108	1	0.4256837823762254
1108	2	0.3402515697549653
1108	3	0.4483810201774645
1108	4	0.6037272220694447
1108	5	0.5387286420899784
1108	6	0.3583271120714855
1108	7	0.2747320324671918
1108	8	0.37080034595151545
1108	9	0.22725357854966433
1108	10	0.4146197213043479
1108	11	0.3046081459291443
1108	12	0.2648838461267786
1108	13	0.36401582864634463
1108	14	-1.1209186545305982
1108	15	-0.08982416815620553
1108	16	0.2812492055173621
1108	17	0.3650098517326654
1108	18	0.5211590400120798
1108	19	0.47679139161862594
1108	20	0.6307824282859542
1108	21	0.5666904890848627
1108	22	0.7457140237394313
1108	23	1.005570831013685
1108	24	0.845155699139049
1108	25	0.7319515469237672
1109	1	0.40118205474996316
1109	2	0.36446164036536643
1109	3	0.4053350111333849
1109	4	0.6330855167026073
1109	5	0.6050054691239382
1109	6	0.43970298015917564
1109	7	0.36431032667025676
1109	8	0.4153699306337586
1109	9	0.5693409586108729
1109	10	0.5287049254836518
1109	11	0.3991072400755229
1109	12	0.38257614215812197
1109	13	0.3958987738146149
1109	14	0.7434695408517484
1109	15	0.49648416897575487
1109	16	0.4333507541939028
1109	17	0.5038778643296524
1109	18	0.45664336587119436
1109	19	0.47800240457062415
1109	20	0.6793212699990079
1109	21	0.6638960743184796
1109	22	0.7225231486238428
1109	23	1.072108291471828
1109	24	0.802217003518912
1109	25	0.7265805876143787
1110	1	0.3700859466235329
1110	2	0.21208062052270193
1110	3	0.2566929666417863
1110	4	0.5428813918087982
1110	5	0.49096076554656776
1110	6	0.29850698447642543
1110	7	0.1910052852791253
1110	8	0.28493410774593286
1110	9	0.3959563198971878
1110	10	0.38868683375206814
1110	11	0.1425472820936733
1110	12	0.1015374626866219
1110	13	0.1707906195752571
1110	14	0.5767060141604148
1110	15	0.43993334081205404
1110	16	0.28987046751761625
1110	17	0.3501642058593575
1110	18	0.48041027636697464
1110	19	0.33057063815545007
1110	20	0.4725000890307953
1110	21	0.4909784730260327
1110	22	0.5575146982950372
1110	23	0.7762939457990754
1110	24	0.711821519735615
1110	25	0.4842285155916599
1111	1	-7.331517695448042E-4
1111	2	-0.005706288193731979
1111	3	0.18378917051314758
1111	4	0.34788028112681246
1111	5	0.27643722216291877
1111	6	0.09868791947435085
1111	7	0.12124165439467305
1111	8	0.1663154146677357
1111	9	0.12122311828500898
1111	10	0.04265626204983164
1111	11	0.07012822161688348
1111	12	0.06576928766452994
1111	13	-0.036133674797667936
1111	14	-1.9802217891419431
1111	15	-0.7657052692770492
1111	16	-0.17370932713136966
1111	17	-0.0468819496525299
1111	18	0.03275412365125874
1111	19	0.032894716467509966
1111	20	0.16075756686944942
1111	21	0.26974340755310067
1111	22	0.05506254971980695
1111	23	-2.0796933895174803
1111	24	-0.49329601999431816
1111	25	-0.22566088075804203
1112	1	-0.1043641703913103
1112	2	-0.18290509909242303
1112	3	0.06499827578745965
1112	4	0.1600900855957974
1112	5	-0.5664411363648441
1112	6	-0.8125238517044409
1112	7	-1.0003660098247664
1112	8	-0.6597116468221031
1112	9	-0.6088031942060326
1112	10	-0.8345637571081568
1112	11	-0.8761552038725355
1112	12	-0.7639282864315646
1112	13	-0.8567267769808069
1112	14	-0.5772508551097317
1112	15	-0.894766727021846
1112	16	-0.8727456759849588
1112	17	-0.7753889248594774
1112	18	-0.9459714190615927
1112	19	-0.7957490679215553
1112	20	-0.8866310295892532
1112	21	-1.80946074248682
1112	22	-1.650418592084172
1112	23	-1.4159474354114323
1112	24	-1.6462330595482086
1112	25	-1.8570083481349542
1113	1	-0.035903200031434324
1113	2	-0.1260200026165137
1113	3	-0.22643083885250093
1113	4	-0.005913343499628354
1113	5	-0.11716202886311322
1113	6	-0.09250571785715778
1113	7	-0.13580313026316304
1113	8	-0.011626545404347155
1113	9	0.135162188128497
1113	10	-0.22072465279117637
1113	11	-0.18954288383827134
1113	12	-0.12275319665460671
1113	13	-0.21586467109341356
1113	14	-0.17369121246046149
1113	15	-0.2449276117229715
1113	16	-0.11894504946808837
1113	17	-0.019509426402429142
1113	18	0.008529783455450968
1113	19	-0.16068327266792437
1113	20	-0.7603849967973275
1113	21	-0.7366032407571765
1113	22	-0.19444519846025798
1113	23	-0.38072604049165903
1113	24	-0.09414087206536692
1113	25	-1.2594485242844797
1114	1	-1.0989647042860788
1114	2	-0.8173454201399579
1114	3	-0.5911534511533496
1114	4	-1.6387135925926941
1114	5	-0.8120585465754715
1114	6	-0.7290375566320861
1114	7	-0.6356000107486466
1114	8	-0.5311774255889077
1114	9	-0.5322287709633997
1114	10	-0.6404533670362937
1114	11	-0.5992253319717449
1114	12	-0.6874929445523605
1114	13	-0.7749225058226727
1114	14	-0.6534852091503609
1114	15	-1.538066058987127
1114	16	-0.9438917619411313
1114	17	-1.0785726943697327
1114	18	-0.9791194216200267
1114	19	-1.0555944183288588
1114	20	-2.2057686251972797
1114	21	-7.052335390480497
1114	22	-7.904655715537345
1114	23	-8.038353470820827
1114	24	-6.087611791703598
1114	25	-7.384526470353325
1115	1	-1.1251674405320067
1115	2	-1.03164383699513
1115	3	-0.8130434112274472
1115	4	-0.689669411279759
1115	5	-0.8873649806912525
1115	6	-0.9003611418759263
1115	7	-1.1426557651587146
1115	8	-0.8239989592074863
1115	9	-2.7582693284545243
1115	10	-1.7186035948017206
1115	11	-1.2237735682685909
1115	12	-1.1155099535293616
1115	13	-1.5368316665899258
1115	14	-1.1537092147846053
1115	15	-2.1065773857479613
1115	16	-1.4531598708965277
1115	17	-3.2044258582166307
1115	18	-2.9282784568646605
1115	19	-2.270295633293628
1115	20	-1.9682197552540188
1115	21	-1.492549246882625
1115	22	-1.0994735517569652
1115	23	-0.48872273982301095
1115	24	-1.573096344328036
1115	25	-1.3349275614730374
1116	1	-1.4583329651181387
1116	2	-0.8558922433570615
1116	3	-0.6764555490664748
1116	4	-0.32035431360772293
1116	5	-0.32314602856957464
1116	6	-0.4103087103730019
1116	7	-0.8039821652232462
1116	8	-1.862409761722489
1116	9	-0.9156659193672425
1116	10	-2.6060196101875555
1116	11	-1.2230733255605841
1116	12	-0.794358408847117
1116	13	-0.7826125776051001
1116	14	-1.2797815062196136
1116	15	-1.2742392574002892
1116	16	-0.9130296191721072
1116	17	-0.7603002564477563
1116	18	-1.4837418821276316
1116	19	-0.9217332675653509
1116	20	-1.121299343513165
1116	21	-0.5782773319544887
1116	22	-0.17395257655176266
1116	23	-0.06024901296413532
1116	24	-0.1305989294870855
1116	25	-0.057829173950977975
1201	1	0.36892316990499197
1201	2	0.4927620739547329
1201	3	0.3989652488191098
1201	4	0.5639510274347899
1201	5	0.48564224483053664
1201	6	0.42055468202250523
1201	7	0.3865581917644434
1201	8	0.5466285611767887
1201	9	0.83722526672274
1201	10	0.7290787219928774
1201	11	0.5176474922446062
1201	12	0.5958215362395407
1201	13	0.5725115631058628
1201	14	1.2262307859651003
1201	15	0.9606749009004929
1201	16	0.9550254828701412
1201	17	0.8800873252733368
1201	18	0.7564042855874362
1201	19	0.8113011017972767
1201	20	0.9206201786882033
1201	21	0.9412806107552072
1201	22	1.0090909930092877
1201	23	0.7980465425854248
1201	24	0.8498651663013206
1201	25	0.8361249314014358
1202	1	-0.2644905781017144
1202	2	0.06357750416280084
1202	3	-0.021652891319821066
1202	4	-0.0379169301159763
1202	5	-0.014711682171380858
1202	6	-0.005459322537170408
1202	7	-0.049745666053155446
1202	8	0.15761190799793923
1202	9	0.15558261443093357
1202	10	0.38692324072462014
1202	11	0.19696225451196625
1202	12	0.16712440570977805
1202	13	0.27250864169522515
1202	14	0.45893372061693327
1202	15	0.4938798818676915
1202	16	0.5209793443110442
1202	17	0.25927077582353064
1202	18	0.26296038634651353
1202	19	0.29061948737263776
1202	20	0.45818183657736
1202	21	0.46896733157799986
1202	22	0.4991367605112842
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2211	7	0.24330755510267466
2211	8	0.2763343823568576
2211	9	0.45855001867460743
2211	10	0.4355770474864187
2211	11	0.24952069919670805
2211	12	0.31365487405818726
2211	13	0.18869769843121986
2211	14	0.5570337381437724
2211	15	0.33514665742755767
2211	16	0.23066029556619583
2211	17	0.3235404069230654
2211	18	0.28144560243692496
2211	19	0.27489643495820815
2211	20	0.3904689927925844
2211	21	0.41682832523345326
2211	22	0.44025390481659343
2211	23	0.5058126844943871
2211	24	0.49392119859698624
2211	25	0.6057011692538907
2212	1	-0.27228129292719316
2212	2	-0.21493798281770182
2212	3	-0.0960512848143864
2212	4	-0.5012208343066362
2212	5	-0.17028148665465181
2212	6	-0.5247331818015724
2212	7	-0.5243848854558095
2212	8	-0.2604367764986506
2212	9	-0.26449876562645613
2212	10	-0.579700850037284
2212	11	-0.4861358258790105
2212	12	-0.5705458833457797
2212	13	-0.4917953748868129
2212	14	-0.39808679480977105
2212	15	-0.6036979324625236
2212	16	-0.3654985863951268
2212	17	-0.37030881362248635
2212	18	-0.30488641309732856
2212	19	-0.47179675017566325
2212	20	-0.43169304005038356
2212	21	-0.4102481898034327
2212	22	-0.4848107680162812
2212	23	0.024444345597324002
2212	24	-0.3334987070079123
2212	25	-0.955236381313803
2213	1	-0.07630836920788653
2213	2	-1.1959018164642146
2213	3	-0.22447443087746421
2213	4	0.19504198444265342
2213	5	0.0723189379635798
2213	6	0.11053593577724996
2213	7	-0.00861145835828836
2213	8	0.1337133713713854
2213	9	0.1675125094559533
2213	10	-0.30549497628477695
2213	11	-0.05011784185668233
2213	12	0.035978063461215015
2213	13	-0.3408064940712876
2213	14	0.09940008016768331
2213	15	9.350631750209004E-4
2213	16	0.029142826936485733
2213	17	0.20012191499419885
2213	18	0.197908668460812
2213	19	0.2190739072218051
2213	20	0.28909705309877154
2213	21	-0.39357830890450884
2213	22	-0.8193108523052075
2213	23	0.02509024462079168
2213	24	0.1611813005920908
2213	25	-0.6340783321763155
2214	1	-0.34406713229682495
2214	2	-0.48533578740564565
2214	3	-0.5233314430773852
2214	4	-0.6106478765859151
2214	5	-0.35760793093017185
2214	6	-0.23932934167094544
2214	7	-0.4688841540143045
2214	8	-0.19860564518875634
2214	9	-0.1513462984012932
2214	10	-1.9950303173400314
2214	11	-0.9814069444496454
2214	12	-0.5563306863050528
2214	13	-0.4100938919877848
2214	14	-0.8806221672145966
2214	15	-0.6258435019610005
2214	16	-0.8335040631404311
2214	17	-0.635566704448479
2214	18	-0.5994020185588127
2214	19	-1.5598004439197837
2214	20	-2.646447567671281
2214	21	-1.9726554501900964
2214	22	-2.250259325758549
2214	23	-2.633996824693078
2214	24	-1.7726591927917141
2214	25	-2.089653483779742
2215	1	-0.27631605713412455
2215	2	-0.38902563704262505
2215	3	-0.5740975458611182
2215	4	-0.2965103677304697
2215	5	-0.19564852103249564
2215	6	-0.14504995036372748
2215	7	-0.24035339694792413
2215	8	-0.27531023026278945
2215	9	-0.2890737291680523
2215	10	-0.3342036327999054
2215	11	-0.5755366467278336
2215	12	-0.4620325060003694
2215	13	-0.31887949859488884
2215	14	-0.2476256653441169
2215	15	-0.6316405004879826
2215	16	-0.3652398855758463
2215	17	-0.2900120626879428
2215	18	-0.24031851490874345
2215	19	-1.9748470267678329
2215	20	-2.4121556417918057
2215	21	-3.390918729499937
2215	22	-2.487892076054216
2215	23	-3.3100790900376893
2215	24	-2.1112143200920954
2215	25	-1.4302341654831494
2216	1	-1.5516996915549939
2216	2	-1.2425944770357482
2216	3	-0.883817114765872
2216	4	-0.991083984110162
2216	5	-2.0783692597492944
2216	6	-1.543396687966336
2216	7	-1.202593126222368
2216	8	-5.182371610939882
2216	9	-4.185887008402769
2216	10	-2.180428145385143
2216	11	-1.123218318371805
2216	12	-1.2449867089797806
2216	13	-1.9694477582483287
2216	14	-3.5443336697975383
2216	15	-2.0336054360154776
2216	16	-3.3063192177617893
2216	17	-2.8789605353318564
2216	18	-2.7592974491593267
2216	19	-1.7470018471157545
2216	20	-0.9215353093127945
2216	21	-0.8047544759338336
2216	22	-0.7105370302123646
2216	23	-1.1339032379803058
2216	24	-0.6364283774303203
2216	25	-0.5043889576028207
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	4.0
7	63.0
8	325.0
9	973.0
10	1949.0
11	3788.0
12	6550.0
13	9910.0
14	13829.0
15	19161.0
16	23627.0
17	26145.0
18	26972.0
19	27948.0
20	29337.0
21	31615.0
22	33357.0
23	35154.0
24	37966.0
25	41292.0
26	43910.0
27	48804.0
28	55609.0
29	65203.0
30	78364.0
31	95820.0
32	128698.0
33	181023.0
34	262590.0
35	412969.0
36	711650.0
37	2265659.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.258607638179534	19.83071242261666	12.26517976500387	23.64550017419993
2	12.736617130657555	22.185135722393245	38.2602158616509	26.8180312852983
3	20.676752349840434	28.529040889628536	27.148409647657957	23.645797112873073
4	12.838649759596995	19.121632201945726	34.59091017926769	33.448807859189586
5	12.359663170420495	39.347643773606656	34.688543666774166	13.604149389198689
6	32.48996030732953	33.78324009563529	18.251530980304086	15.47526861673109
7	24.72488826896123	33.300976386066544	23.14872642716594	18.825408917806293
8	25.93361461751227	36.253457439596495	21.541903659462537	16.271024283428694
9	26.000823683092168	18.121239880328677	20.99423718429189	34.883699252287265
10	15.956971041655944	31.713720188896254	31.973832813976678	20.355475955471125
11	29.20709917266428	27.87819699623661	20.150217829199196	22.764486001899915
12	23.09863815727311	30.5768033791631	29.149285775118205	17.175272688445588
13	27.670548256462464	22.946795066676977	23.976805797279532	25.40585087958103
14	20.960088199035937	26.121888474573186	26.37236339816276	26.545659928228122
15	29.63041036372966	26.03616322687777	21.913437570045385	22.41998883934719
16	19.367612379540198	29.980892528044173	30.050530248765767	20.600964843649862
17	24.158203648024298	30.06717591666695	27.844680162300133	17.929940273008622
18	24.45486591687713	27.266248305939893	28.874750035433056	19.404135741749926
19	22.191378706562354	30.774453772824835	29.53650903142063	17.49765848919219
20	23.295816260618345	28.000502515650123	27.557814192507983	21.14586703122355
21	19.885660225902047	30.153744252320962	29.602505603238356	20.358089918538635
22	21.150715659273676	29.48100656612878	24.25806137008839	25.11021640450915
23	18.414216729734907	30.492746445308722	27.79460728357642	23.298429541379946
24	20.78302838940285	33.89180401219267	25.322146547655063	20.003021050749414
25	22.03115658205832	26.98260473000516	23.534038950186158	27.45219973775036
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9592.0
1	9592.0
2	12002.5
3	14413.0
4	14413.0
5	14413.0
6	17124.5
7	19836.0
8	19836.0
9	19836.0
10	21140.0
11	22444.0
12	22444.0
13	22444.0
14	28777.5
15	35111.0
16	35111.0
17	35111.0
18	51116.0
19	67121.0
20	67121.0
21	67121.0
22	94825.0
23	122529.0
24	122529.0
25	122529.0
26	160019.5
27	197510.0
28	197510.0
29	197510.0
30	238944.5
31	280379.0
32	280379.0
33	280379.0
34	317175.5
35	353972.0
36	353972.0
37	353972.0
38	386730.0
39	419488.0
40	419488.0
41	419488.0
42	463338.0
43	507188.0
44	507188.0
45	507188.0
46	789439.0
47	1071690.0
48	1071690.0
49	1071690.0
50	928765.0
51	785840.0
52	785840.0
53	785840.0
54	578396.5
55	370953.0
56	370953.0
57	370953.0
58	286564.5
59	202176.0
60	202176.0
61	202176.0
62	163938.0
63	125700.0
64	125700.0
65	125700.0
66	96022.5
67	66345.0
68	66345.0
69	66345.0
70	48286.5
71	30228.0
72	30228.0
73	30228.0
74	21048.0
75	11868.0
76	11868.0
77	11868.0
78	7946.5
79	4025.0
80	4025.0
81	4025.0
82	2686.5
83	1348.0
84	1348.0
85	1348.0
86	848.5
87	349.0
88	349.0
89	349.0
90	227.5
91	106.0
92	106.0
93	106.0
94	66.5
95	27.0
96	27.0
97	27.0
98	26.5
99	26.0
100	26.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03279477588541658
2	0.014130565578535436
3	0.019087915421679806
4	0.019003174398720072
5	0.015380495667191496
6	0.005275128679243364
7	0.0
8	1.694820459194655E-4
9	4.237051147986638E-5
10	2.118525573993319E-5
11	0.001101633298476526
12	8.89780741077194E-4
13	0.0025210454330520495
14	6.567429279379288E-4
15	0.0023303781313926512
16	0.002563415944531916
17	0.002542230688791983
18	0.002012599295293653
19	4.44890370538597E-4
20	2.754083246191315E-4
21	0.08039804553304646
22	0.07698721935891721
23	0.07796174112295415
24	0.001101633298476526
25	0.2171065008228353
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4720264.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.91409933335415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.8191136081693	52.916121811455085
2	7.581399231439557	8.933026148136896
3	1.321264979878865	2.335234088107969
4	0.4302650700662619	1.0139471631022534
5	0.21571336866727675	0.6354279414598194
6	0.12473720387015386	0.4409268011622655
7	0.07923256940113486	0.3267540824894736
8	0.0614808291036608	0.28976701383280346
9	0.04907990841890044	0.26023487398767225
>10	0.2681412238937463	2.9609181690028676
>50	0.02503368510058765	1.0455844390369053
>100	0.01831750252986817	2.1479514700275737
>500	0.0024151416917309316	1.0223888457798176
>1k	0.0023785486056950816	3.314291239404848
>5k	2.9274444377785594E-4	1.3381988493419004
>10k+	0.0011343847196391918	21.01922706367199
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	153027	3.241916130114756	No Hit
CCCATGTACTCTGCGTTGATACCAC	148500	3.146010477380079	No Hit
GAGTACATGGGAAGCAGTGGTATCA	68294	1.4468258555029974	No Hit
GCGTTGATACCACTGCTTCCCATGT	52001	1.101654483732266	No Hit
CATGTACTCTGCGTTGATACCACTG	51979	1.1011884081059873	No Hit
GTATCAACGCAGAGTACATGGGAAG	43642	0.9245669310021644	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	39793	0.8430248816591615	No Hit
GCTTCCCATGTACTCTGCGTTGATA	37912	0.8031754156123471	No Hit
ACTCTGCGTTGATACCACTGCTTCC	32462	0.6877157718297112	No Hit
TATCAACGCAGAGTACATGGGAAGC	32046	0.678902705441899	No Hit
GGTATCAACGCAGAGTACATGGGAA	26003	0.5508802050054827	No Hit
GTGGTATCAACGCAGAGTACATGGG	21892	0.46378761865861734	No Hit
CAGTGGTATCAACGCAGAGTACATG	21789	0.4616055373174043	No Hit
GCAGTGGTATCAACGCAGAGTACAT	18941	0.4012699289700746	No Hit
ATACCACTGCTTCCCATGTACTCTG	17978	0.3808685276925189	No Hit
CATGGGAAGCAGTGGTATCAACGCA	15606	0.3306171010773974	No Hit
GTACTCTGCGTTGATACCACTGCTT	15533	0.32907057740838225	No Hit
ACATGGGAAGCAGTGGTATCAACGC	15043	0.318689802095815	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	14952	0.31676194382348105	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	14783	0.3131816356034324	No Hit
GTACATGGTAAGCAGTGGTATCAAC	14367	0.3043685692156201	No Hit
TACCACTGCTTCCCATGTACTCTGC	13554	0.28714495629905445	No Hit
GATACCACTGCTTCCCATGTACTCT	13374	0.28333161026586645	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	13341	0.2826324968264487	No Hit
CTTCCCATGTACTCTGCGTTGATAC	12964	0.27464565541249386	No Hit
CCATGTACTCTGCGTTGATACCACT	12151	0.2574220424959282	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	11024	0.23354625927702347	No Hit
GTTGATACCACTGCTTCCCATGTAC	11014	0.23333440671962413	No Hit
CCACTGCTTCCCATGTACTCTGCGT	10793	0.22865246520109894	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	10145	0.2149244194816222	No Hit
ACCATGTACTCTGCGTTGATACCAC	10084	0.2136321188814863	No Hit
TTCCCATGTACTCTGCGTTGATACC	9753	0.20661979923156842	No Hit
GTACATGGGTAAGCAGTGGTATCAA	9067	0.19208671379397424	No Hit
TGATACCACTGCTTCCCATGTACTC	9063	0.1920019727710145	No Hit
ATCAACGCAGAGTACATGGGAAGCA	7961	0.16865582094560813	No Hit
CCCCATGTACTCTGCGTTGATACCA	7180	0.1521101362127203	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	7051	0.14937723822226892	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	6985	0.14797901134343333	No Hit
AAAAAGTACTCTGCGTTGATACCAC	5013	0.10620168702428509	No Hit
GCTTACCATGTACTCTGCGTTGATA	4957	0.10501531270284883	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.118525573993319E-5	0.0
10	0.0	0.0	0.0	6.355576721979957E-5	0.0
11	0.0	0.0	0.0	1.2711153443959914E-4	0.0
12	0.0	0.0	0.0	1.906673016593987E-4	0.0
13	0.0	0.0	0.0	2.3303781313926508E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGTCA	40	0.0052775014	14.25151	1
TACGTCT	65	8.045164E-4	11.689459	4
CCGCTCC	60	0.0058949124	11.079224	9
GATACGA	110	6.0120565E-6	10.369461	18
GTACAAA	935	0.0	10.161505	1
TAGAACG	85	6.6130457E-4	10.056373	4
TATTCCG	165	1.0282747E-8	9.78539	5
GTCTGCG	175	2.5956979E-9	9.767806	9
CGCAGAT	315	0.0	9.647113	8
CGCCCTA	80	0.004497831	9.505238	16
GTAGGAC	270	0.0	9.498188	3
TGGACCG	80	0.0045278156	9.497584	5
CCGTGTC	130	4.2636148E-6	9.496478	9
AACCGTG	100	2.768092E-4	9.496378	7
GTCCTAG	400	0.0	9.263481	1
AAGCGGT	195	1.6971171E-9	9.252978	10
CGCATAG	175	2.588422E-8	9.247091	19
GGTATCG	155	4.2317515E-7	9.198715	17
GTGTAGG	430	0.0	9.0591	1
CCGCCCG	105	4.5124564E-4	9.044265	9
>>END_MODULE