FastQCFastQC Report
Wed 25 May 2016
SRR1294686_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294686_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4720264
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1682223.563826091083041No Hit
GTACATGGGAAGCAGTGGTATCAAC1464643.1028772966935745No Hit
GAGTACATGGGAAGCAGTGGTATCA737121.5616075711019552No Hit
CATGTACTCTGCGTTGATACCACTG618451.3102021412361682No Hit
GCGTTGATACCACTGCTTCCCATGT557651.1813957863373743No Hit
ACGCAGAGTACATGGGAAGCAGTGG484671.026785789947342No Hit
GTATCAACGCAGAGTACATGGGAAG442570.9375958632822232No Hit
GCTTCCCATGTACTCTGCGTTGATA421210.892344157021726No Hit
ACTCTGCGTTGATACCACTGCTTCC393670.8339999627139499No Hit
TATCAACGCAGAGTACATGGGAAGC366020.7754227305930346No Hit
GGTATCAACGCAGAGTACATGGGAA280470.5941828677379062No Hit
CAGTGGTATCAACGCAGAGTACATG255420.5411138021093735No Hit
GTGGTATCAACGCAGAGTACATGGG226050.47889270600118977No Hit
ATACCACTGCTTCCCATGTACTCTG212510.4502078697293202No Hit
CATGGGAAGCAGTGGTATCAACGCA195320.41379041511237513No Hit
GCAGTGGTATCAACGCAGAGTACAT188930.4002530366945578No Hit
ACATGGGAAGCAGTGGTATCAACGC183490.3887282575720341No Hit
CTGCTTCCCATGTACTCTGCGTTGA171800.36396269361205225No Hit
GGGAAGCAGTGGTATCAACGCAGAG170130.3604247559034834No Hit
GTACTCTGCGTTGATACCACTGCTT166390.3525014702567484No Hit
CTTCCCATGTACTCTGCGTTGATAC161310.3417393603408623No Hit
GATACCACTGCTTCCCATGTACTCT160180.33934542644224985No Hit
TACCACTGCTTCCCATGTACTCTGC159120.3370997893338169No Hit
CCATGTACTCTGCGTTGATACCACT152630.32335055835860027No Hit
GTACATGGTAAGCAGTGGTATCAAC132860.28146730776075235No Hit
AAGCAGTGGTATCAACGCAGAGTAC131150.27784462902922374No Hit
CCACTGCTTCCCATGTACTCTGCGT129680.2747303964354536No Hit
GCAGAGTACATGGGAAGCAGTGGTA129460.27426432080917507No Hit
ATGGGAAGCAGTGGTATCAACGCAG122560.25964649434862114No Hit
ACCATGTACTCTGCGTTGATACCAC118880.25185032023632575No Hit
GTTGATACCACTGCTTCCCATGTAC116830.24750734280963946No Hit
GTATCAACGCAGAGTACTTTTTTTT107830.2284406126436996No Hit
TTCCCATGTACTCTGCGTTGATACC103510.21928858216404848No Hit
TGATACCACTGCTTCCCATGTACTC98420.20850528699242246No Hit
GTACATGGGTAAGCAGTGGTATCAA94680.20058200134568743No Hit
ATCAACGCAGAGTACATGGGAAGCA92420.19579413354846253No Hit
CCCCATGTACTCTGCGTTGATACCA85750.1816635679699271No Hit
TATCAACGCAGAGTACTTTTTTTTT82030.17378265283467195No Hit
GGTAAGCAGTGGTATCAACGCAGAG80580.17071079075238166No Hit
GGTATCAACGCAGAGTACTTTTTTT71970.15247028556029915No Hit
AAAAAGTACTCTGCGTTGATACCAC59400.12584041909520313No Hit
ACGCAGAGTACTTTTTTTTTTTTTT56140.11893402572398493No Hit
GCTTACCATGTACTCTGCGTTGATA55470.11751461358940941No Hit
GGAAGCAGTGGTATCAACGCAGAGT54680.1158409783859547No Hit
GTACTTTTTTTTTTTTTTTTTTTTT50370.10671013316204349No Hit
GAGTACATGGTAAGCAGTGGTATCA49690.10526953577172803No Hit
CTGCGTTGATACCACTGCTTCCCAT47990.10166804229593937No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAT602.5389845E-514.2686242
GTCGATA602.5392386E-514.2684733
TCGCGCG400.00529937314.2426289
TCGATAC604.0502654E-412.6830874
TCTACGC701.07868516E-412.2301193
GCGTCGA657.9144223E-411.7123121
CGGTACT657.941955E-411.7074644
TACGCAG752.050699E-411.4147785
CGATACC700.001476336410.8712175
CGGGTTA1158.88811E-710.73357514
CTACGCA909.399319E-510.5692394
TACACCG909.399319E-510.5692395
AATACCG750.002620961710.1464695
CACGTAC2002.5465852E-119.987933
TAGACTG5550.09.9407975
AAAAGTA13650.09.8260132
AGTACTC16750.09.6542895
GTCTAGG3950.09.6367131
TATACTG7650.09.5744875
TACGACT900.00110127239.5123154
Produced by FastQC (version 0.11.5)