FastQCFastQC Report
Wed 25 May 2016
SRR1294685_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294685_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4728338
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC93270.19725747186432105No Hit
CCCATGTACTCTGCGTTGATACCAC87300.18463147093122362No Hit
GTATCAACGCAGAGTACTTTTTTTT52400.11082118071931407No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC909.530368E-510.5548153
TCGCGCG1701.5752448E-910.0539699
TATCGCG1752.5993359E-99.7668167
CTAGCGC800.00452094349.4993333
TGGACCG1606.6640496E-89.4983295
CCGCGCA800.00453635269.4954179
GTATTAG7250.09.4360171
GTCCTAG6000.09.3431851
ATCGCGC2357.2759576E-129.2934848
GTACTGG11250.09.2059411
TGCACCG2002.6702764E-99.0234125
ACTATCG5300.08.95822311
CTGTCGC3100.08.8828099
CTAATAC6100.08.8764273
CGCCCTA2252.6011548E-108.87186316
ACCGTCT1304.3970234E-58.7650918
TAGACTG5650.08.7418255
TGTATCG2659.094947E-128.6022615
TACGACA1554.2181473E-68.57967954
CCGTAAG1000.0029023978.5513911
Produced by FastQC (version 0.11.5)