##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294685_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4728338 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.953362259635416 33.0 33.0 33.0 27.0 33.0 2 31.052546581906793 33.0 33.0 33.0 27.0 33.0 3 30.87733850668036 33.0 33.0 33.0 27.0 33.0 4 34.2843409248662 37.0 37.0 37.0 27.0 37.0 5 34.57323440075562 37.0 37.0 37.0 27.0 37.0 6 34.772005935277896 37.0 37.0 37.0 27.0 37.0 7 34.728378555001775 37.0 37.0 37.0 27.0 37.0 8 34.6487133534024 37.0 37.0 37.0 27.0 37.0 9 34.35239105157034 37.0 37.0 37.0 27.0 37.0 10 34.43571377511506 37.0 37.0 37.0 27.0 37.0 11 34.69320869193361 37.0 37.0 37.0 27.0 37.0 12 34.60969837604672 37.0 37.0 37.0 27.0 37.0 13 34.54451331524946 37.0 37.0 37.0 27.0 37.0 14 35.63709743254395 37.0 37.0 40.0 27.0 40.0 15 35.95727483948905 40.0 37.0 40.0 27.0 40.0 16 35.96878141960241 40.0 37.0 40.0 27.0 40.0 17 35.89787172575226 37.0 37.0 40.0 27.0 40.0 18 35.89973707463383 37.0 37.0 40.0 27.0 40.0 19 35.98203322182128 40.0 37.0 40.0 27.0 40.0 20 35.87792877751125 40.0 37.0 40.0 27.0 40.0 21 35.82316619497168 40.0 37.0 40.0 27.0 40.0 22 35.662971428861475 37.0 37.0 40.0 27.0 40.0 23 35.26097563245267 37.0 33.0 40.0 27.0 40.0 24 35.57701289544021 37.0 37.0 40.0 27.0 40.0 25 34.35475319234792 37.0 33.0 40.0 22.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.38912827514449333 1101 2 0.5757666242743618 1101 3 0.29698630730050724 1101 4 0.32494226118429737 1101 5 0.41609508764523184 1101 6 0.22942910570766628 1101 7 0.32668326418239246 1101 8 0.3841502809943407 1101 9 0.6864322783208365 1101 10 0.6346611801640663 1101 11 0.41872530546392284 1101 12 0.4913747856912849 1101 13 0.40825295969818143 1101 14 1.0735552529127048 1101 15 0.7849005860930234 1101 16 0.8105251578738262 1101 17 0.8391026341261352 1101 18 0.7929574543839593 1101 19 0.6911371169371918 1101 20 0.8306333652808391 1101 21 0.9175253685674747 1101 22 0.9251220935082074 1101 23 1.2541847299123248 1101 24 1.0223392421873996 1101 25 0.9824662986155275 1102 1 0.09438502677161154 1102 2 0.4152285783445713 1102 3 0.08160173741907428 1102 4 -0.03186312068462627 1102 5 0.10476130524386207 1102 6 0.1592612816335901 1102 7 0.18231141571365583 1102 8 0.2379438989925049 1102 9 0.4164867605433784 1102 10 0.4637500780141792 1102 11 0.28447850633225613 1102 12 0.31431272267676746 1102 13 0.35001526728360943 1102 14 0.8616062171000323 1102 15 0.591551814787465 1102 16 0.3419351257745191 1102 17 0.536403405496138 1102 18 0.5127792770765964 1102 19 0.5132570905972358 1102 20 0.6264792090857156 1102 21 0.5774742644239694 1102 22 0.6177939904078258 1102 23 1.0210751250921888 1102 24 0.7689623588202323 1102 25 0.5128925457358591 1103 1 0.25070451678327643 1103 2 0.5400402614485742 1103 3 0.125609958574195 1103 4 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2212 4 -0.447471572468352 2212 5 -0.15425989195668421 2212 6 -0.491027973445064 2212 7 -0.4223004076481658 2212 8 -0.20346960899709643 2212 9 -0.16641883526982326 2212 10 -0.5580476002033379 2212 11 -0.46959661289231747 2212 12 -0.7270568502824801 2212 13 -0.6648273214380538 2212 14 -0.47569868813965854 2212 15 -0.6311441122223229 2212 16 -0.44382740828964273 2212 17 -0.5178345830149453 2212 18 -0.4780853333532278 2212 19 -0.5674227217401864 2212 20 -0.5027991338475175 2212 21 -0.5266856281190755 2212 22 -0.519148305671699 2212 23 9.07591337700353E-4 2212 24 -0.19599171262160553 2212 25 -0.7644545913259506 2213 1 -0.04938916697976481 2213 2 -1.235626650461807 2213 3 -0.25589441748298114 2213 4 0.20478661511214113 2213 5 0.15564603630505047 2213 6 0.09730115021392294 2213 7 0.1536399361989922 2213 8 0.26580026164545956 2213 9 0.2313492092071172 2213 10 -0.3232651359883931 2213 11 0.05494697609010757 2213 12 0.018620410414136757 2213 13 -0.2183614216326788 2213 14 0.09699996683661993 2213 15 -0.009880277490204037 2213 16 0.07459534379852784 2213 17 0.22328813494758037 2213 18 0.22833773602865648 2213 19 0.27213915708969694 2213 20 0.2892510586078245 2213 21 -0.3642247947233699 2213 22 -0.707599032747936 2213 23 -0.0892015522046492 2213 24 0.16708480590934727 2213 25 -0.5590864072736039 2214 1 -0.1677393675204506 2214 2 -0.4155521286670094 2214 3 -0.4575588344203254 2214 4 -0.48390294235213105 2214 5 -0.15598667147093437 2214 6 -0.1205458064850049 2214 7 -0.4415520833850408 2214 8 0.025211488332971044 2214 9 -0.0018364563639465814 2214 10 -1.8333339144548333 2214 11 -0.7998097026305899 2214 12 -0.5039584892966218 2214 13 -0.2788966448951271 2214 14 -0.8249320836191529 2214 15 -0.5581643939105732 2214 16 -0.7024483703972635 2214 17 -0.49629081304189526 2214 18 -0.4616915560244408 2214 19 -1.2970695038722937 2214 20 -2.4021149387186327 2214 21 -1.915061386976305 2214 22 -2.024081262212455 2214 23 -2.499140304838491 2214 24 -1.5814539855377987 2214 25 -1.8233608748008336 2215 1 -0.19539578835652804 2215 2 -0.4145562276107455 2215 3 -0.7735381576174412 2215 4 -0.239376511226574 2215 5 -0.20087460706868399 2215 6 -0.13670599088250412 2215 7 -0.2472650028869765 2215 8 -0.22630882368350314 2215 9 -0.22228935877897982 2215 10 -0.34962017215869423 2215 11 -0.740630954426635 2215 12 -0.49900536313074184 2215 13 -0.2961315586920179 2215 14 -0.17277567062199495 2215 15 -0.7081128814504893 2215 16 -0.4008128457238129 2215 17 -0.19971293893318176 2215 18 -0.24103463017954851 2215 19 -2.2613543608483724 2215 20 -2.400609458855854 2215 21 -3.3317897892300365 2215 22 -2.3928217111674357 2215 23 -3.4986993897960907 2215 24 -2.6687214825838907 2215 25 -1.8687902327982187 2216 1 -1.41444479395415 2216 2 -1.1041036131847868 2216 3 -0.6099818868723439 2216 4 -0.617570355352882 2216 5 -1.645401612928076 2216 6 -1.4417313489579442 2216 7 -0.9346252820864436 2216 8 -5.203848880106978 2216 9 -3.938139289475714 2216 10 -1.8935775453814259 2216 11 -0.8987950291481042 2216 12 -0.9932462674692033 2216 13 -1.7755213301038282 2216 14 -3.5084208777982013 2216 15 -1.8991637998408422 2216 16 -2.684120079162774 2216 17 -2.5432744800370983 2216 18 -2.42676018308142 2216 19 -1.4253842544551887 2216 20 -0.6492322614550048 2216 21 -0.5237807562092769 2216 22 -0.4952628455468826 2216 23 -0.8239424241779361 2216 24 -0.39302018094097946 2216 25 -0.09220229558643211 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 5.0 7 116.0 8 591.0 9 1569.0 10 3173.0 11 5750.0 12 9341.0 13 13570.0 14 17630.0 15 21472.0 16 23933.0 17 25615.0 18 26865.0 19 27944.0 20 29437.0 21 31630.0 22 33666.0 23 36550.0 24 39074.0 25 41930.0 26 45326.0 27 51405.0 28 58601.0 29 68876.0 30 83248.0 31 101018.0 32 134168.0 33 187968.0 34 267892.0 35 417015.0 36 711684.0 37 2211276.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.51225256607352 20.231738350727497 13.673920857107378 23.582088226091603 2 15.527734193060391 19.746062674847103 40.79832019869273 23.927882933399776 3 19.04994485450431 24.858011453848487 28.22237320660798 27.869670485039222 4 12.954662615373127 16.08383842294007 36.86609429876471 34.0954046629221 5 14.417860261417555 36.80840181272973 34.385195758564436 14.388542167288284 6 33.10384298858738 37.07576523160998 16.4048406804296 13.415551099373035 7 28.09203608249603 31.65375709878188 21.827300267581673 18.426906551140416 8 25.05208574568959 37.16630702069958 19.878365428642795 17.903241804968044 9 24.515597162275796 17.469760776398093 21.328233257816347 36.686408803509764 10 15.45421091256707 29.96140269990758 32.28316248300133 22.301223904524026 11 33.90010635982699 25.292320329827554 21.846190027724617 18.96138328262084 12 21.73629550672178 26.644946317745227 30.569477093703107 21.049281081829882 13 27.132147572000758 23.18456821116811 26.22343235733408 23.459851859497054 14 21.032884899678745 22.281385949736798 27.325408021047686 29.36032112953677 15 23.61634120750562 30.137473898866872 23.12148508441719 23.124699809210313 16 22.927919144168058 30.43334719338237 24.767089842304223 21.871643820145348 17 21.236166337896773 29.614289149578354 27.525812507891427 21.623732004633446 18 21.491318975924255 29.323761633282885 28.15882598275385 21.02609340803901 19 22.06499721042923 27.96913749178885 28.45909947833546 21.50676581944646 20 23.14518119441603 27.624553250320037 27.737828179528496 21.492437375735435 21 23.295390687058994 27.98193789213745 27.10459136428221 21.618080056521343 22 22.272727368933253 27.65546929748719 28.21791639742621 21.853886936153344 23 22.293096357805055 27.96346227477966 27.980923881996787 21.7625174854185 24 22.208569183121515 27.389404038498466 27.92232374819491 22.479703030185107 25 22.810402933508552 27.41777061828356 27.17126263803811 22.60056381016978 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2439.0 1 2439.0 2 2838.0 3 3237.0 4 3237.0 5 3237.0 6 4380.0 7 5523.0 8 5523.0 9 5523.0 10 8506.0 11 11489.0 12 11489.0 13 11489.0 14 19458.5 15 27428.0 16 27428.0 17 27428.0 18 46678.0 19 65928.0 20 65928.0 21 65928.0 22 99323.5 23 132719.0 24 132719.0 25 132719.0 26 179683.0 27 226647.0 28 226647.0 29 226647.0 30 283118.0 31 339589.0 32 339589.0 33 339589.0 34 391557.0 35 443525.0 36 443525.0 37 443525.0 38 489994.0 39 536463.0 40 536463.0 41 536463.0 42 549943.5 43 563424.0 44 563424.0 45 563424.0 46 574636.0 47 585848.0 48 585848.0 49 585848.0 50 559955.0 51 534062.0 52 534062.0 53 534062.0 54 490116.5 55 446171.0 56 446171.0 57 446171.0 58 392853.5 59 339536.0 60 339536.0 61 339536.0 62 285694.5 63 231853.0 64 231853.0 65 231853.0 66 180543.5 67 129234.0 68 129234.0 69 129234.0 70 95856.5 71 62479.0 72 62479.0 73 62479.0 74 44440.0 75 26401.0 76 26401.0 77 26401.0 78 18169.0 79 9937.0 80 9937.0 81 9937.0 82 6571.5 83 3206.0 84 3206.0 85 3206.0 86 2068.0 87 930.0 88 930.0 89 930.0 90 571.0 91 212.0 92 212.0 93 212.0 94 124.5 95 37.0 96 37.0 97 37.0 98 29.0 99 21.0 100 21.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028953090916935296 2 0.013450815064405293 3 0.017849823764713942 4 0.020281967998057666 5 0.01865348881573187 6 0.006873451094232265 7 0.0013112429779766168 8 0.001628479182325798 9 8.88261372177708E-4 10 5.921742481184721E-4 11 0.0010363049342073261 12 0.0011209012553671078 13 0.0026224859559532336 14 5.921742481184721E-4 15 0.0020091626275448157 16 0.002537889634793452 17 0.002262951591024161 18 0.0019880135472548706 19 2.537889634793452E-4 20 4.018325255089632E-4 21 0.07933020016758531 22 0.07670771421163208 23 0.07797665902902881 24 9.728576933374898E-4 25 0.2207541000664504 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4728338.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.35746491637894 #Duplication Level Percentage of deduplicated Percentage of total 1 80.58957040569675 54.28309161227769 2 12.448900116393329 16.770527056749387 3 3.5148606471625645 7.1025630818164025 4 1.3927096057593922 3.752375536345688 5 0.6379022477334348 2.1483739135892055 6 0.36289579700370994 1.466624454898726 7 0.2388422474203574 1.1261465810816076 8 0.1501517399825835 0.8091072446408097 9 0.10425976048624407 0.632040584322803 >10 0.5059242518926655 6.051104543022055 >50 0.03470351420932579 1.5981112150663705 >100 0.017131049909543762 1.9628460651491857 >500 0.0012638926480274379 0.5948213295896098 >1k 7.899318767964097E-4 1.2064338539502604 >5k 9.479182521462185E-5 0.4958329275002695 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 9327 0.19725747186432105 No Hit CCCATGTACTCTGCGTTGATACCAC 8730 0.18463147093122362 No Hit GTATCAACGCAGAGTACTTTTTTTT 5240 0.11082118071931407 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 2.114908028994543E-5 0.0 0.0 0.0 6 0.0 2.114908028994543E-5 0.0 0.0 0.0 7 0.0 2.114908028994543E-5 0.0 0.0 0.0 8 0.0 2.114908028994543E-5 0.0 0.0 0.0 9 0.0 2.114908028994543E-5 0.0 2.114908028994543E-5 0.0 10 0.0 2.114908028994543E-5 0.0 2.114908028994543E-5 0.0 11 0.0 2.114908028994543E-5 0.0 4.229816057989086E-5 0.0 12 0.0 2.114908028994543E-5 0.0 1.0574540144972715E-4 0.0 13 0.0 2.114908028994543E-5 0.0 1.2689448173967258E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGAC 90 9.530368E-5 10.554815 3 TCGCGCG 170 1.5752448E-9 10.053969 9 TATCGCG 175 2.5993359E-9 9.766816 7 CTAGCGC 80 0.0045209434 9.499333 3 TGGACCG 160 6.6640496E-8 9.498329 5 CCGCGCA 80 0.0045363526 9.495417 9 GTATTAG 725 0.0 9.436017 1 GTCCTAG 600 0.0 9.343185 1 ATCGCGC 235 7.2759576E-12 9.293484 8 GTACTGG 1125 0.0 9.205941 1 TGCACCG 200 2.6702764E-9 9.023412 5 ACTATCG 530 0.0 8.958223 11 CTGTCGC 310 0.0 8.882809 9 CTAATAC 610 0.0 8.876427 3 CGCCCTA 225 2.6011548E-10 8.871863 16 ACCGTCT 130 4.3970234E-5 8.765091 8 TAGACTG 565 0.0 8.741825 5 TGTATCG 265 9.094947E-12 8.602261 5 TACGACA 155 4.2181473E-6 8.5796795 4 CCGTAAG 100 0.002902397 8.551391 1 >>END_MODULE