FastQCFastQC Report
Wed 25 May 2016
SRR1294685_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294685_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4728338
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT153220.3240462082025439No Hit
GGTATCAACGCAGAGTACTTTTTTT104040.22003503133659227No Hit
TATCAACGCAGAGTACTTTTTTTTT101550.21476891034439585No Hit
CCCATGTACTCTGCGTTGATACCAC96720.2045539045643522No Hit
GTACATGGGAAGCAGTGGTATCAAC93670.19810343507591888No Hit
ACGCAGAGTACTTTTTTTTTTTTTT59040.12486417003183783No Hit
ATTCCATTCCATTCCATTCCATTCC53620.1134013685146874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG2106.548362E-119.5151075
AAACCGT1002.771887E-49.4950826
ATCGACC800.00454202349.4939788
CTAGGAC5050.09.4208983
CTAATAC4600.09.3082573
CGCCCTA2151.07320375E-109.27338416
GTCTAGC1651.0349504E-79.2305861
ACATCGA1451.7204566E-69.16766556
GTCCTAG5350.09.0742261
TATCGCG2106.7666406E-109.0418837
TAAACCG950.00179869629.0143125
ATCGTAC950.00179869629.0143123
TAGACAG6700.08.94704155
CTTAGAC4050.08.9277553
GGACTGT10100.08.83700756
TGAACCG2054.0308805E-98.818885
AACCGTT1401.0949732E-58.8158367
TGGTCGG1401.0949732E-58.81583610
GTACCGT1201.7751342E-48.7038266
AGGACCG1651.0275526E-68.6500985
Produced by FastQC (version 0.11.5)