FastQCFastQC Report
Wed 25 May 2016
SRR1294684_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294684_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3977740
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42280.10629151226575893No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACCT658.056014E-411.68749713
GGTATCA19450.011.430781
GTATTAG5200.010.232091
GTATACG1054.102761E-59.9536661
CGCTATC2600.09.86132512
TGTTCCG1554.0867235E-89.8037725
ACGAAAC1106.806899E-59.50445515
GCGCGCC1106.8737136E-59.49597111
GCCTTAT3050.09.3454681
TGCGCTA2357.2759576E-129.29381410
CAGTGCG1356.891898E-69.1441539
GTATCAA36600.09.1377921
TATACCG1054.5076644E-49.0451465
CACGGGT850.00745186448.9392954
GCGCTAT2451.6370905E-118.91458511
CTAGACA3750.08.86484
TAAGACT5500.08.8072374
TTGTGCG2701.8189894E-128.7925657
GTGCGCT2607.2759576E-128.7654029
GTGTTAC3050.08.7224371
Produced by FastQC (version 0.11.5)