##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294684_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3977740 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.14860222136188 33.0 33.0 33.0 27.0 33.0 2 31.22263898595685 33.0 33.0 33.0 27.0 33.0 3 31.073217455137844 33.0 33.0 33.0 27.0 33.0 4 34.60864837822482 37.0 37.0 37.0 27.0 37.0 5 34.732537068787806 37.0 37.0 37.0 27.0 37.0 6 35.0030281013842 37.0 37.0 37.0 33.0 37.0 7 34.98946939719539 37.0 37.0 37.0 33.0 37.0 8 34.95084998013947 37.0 37.0 37.0 33.0 37.0 9 34.643778628065185 37.0 37.0 37.0 27.0 37.0 10 34.730865767998914 37.0 37.0 37.0 27.0 37.0 11 34.95203733778477 37.0 37.0 37.0 33.0 37.0 12 34.88519511079156 37.0 37.0 37.0 33.0 37.0 13 34.8134880610598 37.0 37.0 37.0 33.0 37.0 14 35.94536244198967 40.0 37.0 40.0 27.0 40.0 15 36.267754805492565 40.0 37.0 40.0 27.0 40.0 16 36.296215941715644 40.0 37.0 40.0 27.0 40.0 17 36.24754106603247 40.0 37.0 40.0 27.0 40.0 18 36.25219446218204 40.0 37.0 40.0 27.0 40.0 19 36.33661878353035 40.0 37.0 40.0 27.0 40.0 20 36.2078700468105 40.0 37.0 40.0 27.0 40.0 21 36.15432305781675 40.0 37.0 40.0 27.0 40.0 22 36.011175441331005 40.0 37.0 40.0 27.0 40.0 23 35.602325692478644 37.0 37.0 40.0 27.0 40.0 24 35.912249418011235 37.0 37.0 40.0 27.0 40.0 25 34.80238778804045 37.0 33.0 40.0 22.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.361087979014858 1101 2 0.6456260777815608 1101 3 0.32700440385192664 1101 4 0.4294949119095719 1101 5 0.4613038316889657 1101 6 0.36487594566394677 1101 7 0.48377460791184745 1101 8 0.4787246181770115 1101 9 0.7230436935199407 1101 10 0.682343203807747 1101 11 0.42961846303244755 1101 12 0.4674927770316657 1101 13 0.4847988379860624 1101 14 1.0853642767436966 1101 15 0.9337571859038647 1101 16 0.8021161468005147 1101 17 0.9261892452415807 1101 18 0.925428446257925 1101 19 0.8983223254732309 1101 20 0.9504222899352541 1101 21 0.8940836459001389 1101 22 0.928240527307608 1101 23 1.3020543697055231 1101 24 1.051320572330937 1101 25 1.0158654678493093 1102 1 0.05748546018055123 1102 2 0.3965591429362867 1102 3 0.1642060371599321 1102 4 0.044627323302961486 1102 5 0.028532894253920915 1102 6 0.11246511449155605 1102 7 0.1070879314725488 1102 8 0.2473055607600685 1102 9 0.44373309522049453 1102 10 0.5746488803147258 1102 11 0.3101119515992181 1102 12 0.3973450624770152 1102 13 0.371243562965077 1102 14 1.0059500470988354 1102 15 0.6969837473369367 1102 16 0.3783464097239033 1102 17 0.605467768981157 1102 18 0.5810586616582825 1102 19 0.6440408054277995 1102 20 0.7211328414714302 1102 21 0.7376529593975576 1102 22 0.7755946198751928 1102 23 1.1467368591041733 1102 24 0.8639079566708432 1102 25 0.6818829718470738 1103 1 0.2568303026079839 1103 2 0.5033730186287144 1103 3 0.15840084622690398 1103 4 0.02830309054851199 1103 5 0.18232032180571878 1103 6 0.18545433363976827 1103 7 0.2898828610800308 1103 8 0.40482680405533955 1103 9 0.5887054786420265 1103 10 0.6421003628221769 1103 11 0.39613773774085814 1103 12 0.40535904626761976 1103 13 0.3618527664071749 1103 14 1.0719674672305146 1103 15 0.7242283584151608 1103 16 0.4216897285550374 1103 17 0.5390019039206564 1103 18 0.6532288330492193 1103 19 0.6657448266886803 1103 20 0.7828024856759725 1103 21 0.7994116723320772 1103 22 0.6911541144684605 1103 23 1.1810963932201375 1103 24 0.8518073866619815 1103 25 0.7400925812093604 1104 1 0.25206349975255904 1104 2 0.500157107493731 1104 3 0.1754389899262101 1104 4 0.23648632722985496 1104 5 0.16356651584956694 1104 6 0.20836333041624044 1104 7 0.187427949299952 1104 8 0.3850886354062766 1104 9 0.5850528485335076 1104 10 0.5369649761633255 1104 11 0.4188880374960249 1104 12 0.4411994329613762 1104 13 0.4210285045938278 1104 14 0.8347405907115188 1104 15 0.6508184159984296 1104 16 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2213 16 0.029937381769713056 2213 17 0.21624313492679192 2213 18 0.14450574346819423 2213 19 0.1826490762443811 2213 20 0.2585336656167385 2213 21 -0.41086975300545703 2213 22 -0.7672242137386291 2213 23 -0.027089964091636887 2213 24 0.09729426138193276 2213 25 -0.5355065312304532 2214 1 -0.10678265348202487 2214 2 -0.3809856355610606 2214 3 -0.3539252712114376 2214 4 -0.3700105360439423 2214 5 -0.2218857598595534 2214 6 -0.14278364224495732 2214 7 -0.4246933165767288 2214 8 -0.08832998770180467 2214 9 -0.032737615413367394 2214 10 -1.943986863168874 2214 11 -0.7884489879974907 2214 12 -0.4824415376537914 2214 13 -0.2109611535414757 2214 14 -0.8372089845489441 2214 15 -0.5902604039493298 2214 16 -0.6640156338263239 2214 17 -0.5670314956074307 2214 18 -0.478727379519043 2214 19 -1.3791671876885019 2214 20 -2.5071880765668126 2214 21 -1.9531170459349383 2214 22 -2.233842411975772 2214 23 -2.514995462156172 2214 24 -1.6879870486106583 2214 25 -1.9306038652065638 2215 1 -0.22597033882460948 2215 2 -0.396124403405004 2215 3 -0.8468480967887864 2215 4 -0.2775891241756909 2215 5 -0.11709403386887374 2215 6 -0.15305208877568788 2215 7 -0.20859752663980657 2215 8 -0.2034293500226525 2215 9 -0.17364831444433548 2215 10 -0.23101115653005166 2215 11 -0.6841171129561445 2215 12 -0.4782676442442906 2215 13 -0.265038762287773 2215 14 -0.18840675425386166 2215 15 -0.612508704765915 2215 16 -0.442876584103459 2215 17 -0.27510919639582454 2215 18 -0.4074512014520977 2215 19 -2.4442682757986773 2215 20 -2.557631820149034 2215 21 -3.5339544564455707 2215 22 -2.5231458258746997 2215 23 -3.6127639446408466 2215 24 -2.4904046637423747 2215 25 -1.8374896208290465 2216 1 -1.5088633457627054 2216 2 -1.1632935678368632 2216 3 -0.7683460209916682 2216 4 -0.8477774887663969 2216 5 -1.7099819431504955 2216 6 -1.5445755222840631 2216 7 -1.1124517176959756 2216 8 -5.13496088816294 2216 9 -4.221600906350936 2216 10 -2.0957227128857454 2216 11 -1.1233178027466977 2216 12 -1.1807175050813825 2216 13 -1.8737568261453745 2216 14 -3.4990482290043374 2216 15 -2.0458923899347567 2216 16 -2.951883163285217 2216 17 -2.797215006013282 2216 18 -2.5577356072058706 2216 19 -1.6396257561997203 2216 20 -0.9422357882875758 2216 21 -0.6848479955814213 2216 22 -0.6574996581625854 2216 23 -0.9111460844993999 2216 24 -0.5200562596210716 2216 25 -0.26067513032508316 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 25.0 8 236.0 9 723.0 10 1607.0 11 3115.0 12 5599.0 13 8467.0 14 11264.0 15 14364.0 16 16530.0 17 17923.0 18 19086.0 19 20357.0 20 21073.0 21 22879.0 22 24827.0 23 26537.0 24 28788.0 25 32049.0 26 35180.0 27 39670.0 28 46079.0 29 54781.0 30 66558.0 31 81611.0 32 109938.0 33 156022.0 34 227493.0 35 356454.0 36 609020.0 37 1919485.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.12066192766667 20.867711285306427 13.891136115188532 24.120490671838375 2 15.192971481214773 20.996070570818233 40.51692058268597 23.294037365281024 3 17.7375018104572 25.590058176024723 30.604924041262333 26.06751597225575 4 12.532742713496825 17.536113481083845 38.58851213237448 31.342631673044846 5 13.125860130600294 38.120032678990775 35.61197797160428 13.142129218804651 6 30.48264844958356 38.69024153792623 17.847287450044146 12.979822562446072 7 26.182570277449425 33.29204516943566 23.331575213425534 17.19380933968938 8 23.991562995337777 37.34113680844404 21.22376791700763 17.44353227921055 9 24.76037696099209 17.296024391948524 21.903655021261 36.03994362579839 10 14.774162537006049 30.12184342418587 34.27046796563265 20.83352607317544 11 32.27908978601213 25.139590648067657 24.44077007277066 18.140549493149564 12 21.070712616769566 26.803288126372337 31.52032855118 20.605670705678097 13 26.43434730447392 23.503530244658375 27.16050872530061 22.901613725567096 14 20.724853885851967 23.075778236332848 28.132001512427244 28.06736636538794 15 22.17086875930352 31.2711415075342 24.338373732703715 22.21961600045856 16 21.830837551072946 30.520152130256 26.16812867114302 21.480881647528033 17 20.67753138414027 29.937530891386515 28.160877061457956 21.22406066301526 18 21.029534065894286 29.530772487432262 28.74226552200247 20.697427924670986 19 21.598613075872805 29.166535729109345 28.758639050532175 20.47621214448568 20 22.14773520039721 28.6625646569333 28.527361745721485 20.662338396948005 21 22.272024815077053 28.23726498720045 28.353334047914153 21.137376149808347 22 22.14862500459179 28.111157015020304 28.438343977057663 21.301874003330244 23 22.257945042657713 28.176809342124198 28.316150147895495 21.249095467322594 24 22.000345929247413 28.285171400902836 28.187099453653026 21.52738321619673 25 22.08336419907065 28.168752182648564 28.247541701500857 21.500341916779924 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2775.0 1 2775.0 2 2843.0 3 2911.0 4 2911.0 5 2911.0 6 4280.0 7 5649.0 8 5649.0 9 5649.0 10 9487.5 11 13326.0 12 13326.0 13 13326.0 14 23725.0 15 34124.0 16 34124.0 17 34124.0 18 56037.0 19 77950.0 20 77950.0 21 77950.0 22 114350.5 23 150751.0 24 150751.0 25 150751.0 26 195794.5 27 240838.0 28 240838.0 29 240838.0 30 288427.0 31 336016.0 32 336016.0 33 336016.0 34 372535.0 35 409054.0 36 409054.0 37 409054.0 38 431902.0 39 454750.0 40 454750.0 41 454750.0 42 463588.0 43 472426.0 44 472426.0 45 472426.0 46 463226.5 47 454027.0 48 454027.0 49 454027.0 50 433199.0 51 412371.0 52 412371.0 53 412371.0 54 376776.5 55 341182.0 56 341182.0 57 341182.0 58 296018.5 59 250855.0 60 250855.0 61 250855.0 62 206557.5 63 162260.0 64 162260.0 65 162260.0 66 125434.5 67 88609.0 68 88609.0 69 88609.0 70 65450.0 71 42291.0 72 42291.0 73 42291.0 74 29775.5 75 17260.0 76 17260.0 77 17260.0 78 11622.0 79 5984.0 80 5984.0 81 5984.0 82 3884.5 83 1785.0 84 1785.0 85 1785.0 86 1109.0 87 433.0 88 433.0 89 433.0 90 259.0 91 85.0 92 85.0 93 85.0 94 51.0 95 17.0 96 17.0 97 17.0 98 14.0 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030997501093585806 2 0.01438002483822472 3 0.02121807860745046 4 0.02265105311056027 5 0.021947135810787027 6 0.005731898012439224 7 1.508394213799796E-4 8 6.284975890832483E-4 9 8.547567211532176E-4 10 3.016788427599592E-4 11 9.553163354065373E-4 12 8.547567211532176E-4 13 0.0028156691990929524 14 7.039172997732381E-4 15 0.0019860523815030647 16 0.0024134307420796736 17 0.0023631509349530135 18 0.0024134307420796736 19 4.273783605766088E-4 20 3.77098553449949E-4 21 0.08502315385118182 22 0.08180524619507559 23 0.08326336060174873 24 0.0012067153710398368 25 0.2247004580490429 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3977740.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.2764511127325 #Duplication Level Percentage of deduplicated Percentage of total 1 75.47259455038599 46.24692750317808 2 14.146706765256495 17.33719971014803 3 4.856524377376395 8.92771735764295 4 2.152170153286993 5.275093967366896 5 1.0876144566937027 3.3322577042546397 6 0.6080075680809469 2.235392761301011 7 0.393142467160776 1.6863262618521433 8 0.2538407934894875 1.2443570378140645 9 0.1810538284953064 0.9984902463509125 >10 0.7916558654490851 8.343572829510496 >50 0.03866291711581039 1.6005410161827147 >100 0.01666350155369759 1.8262416450416903 >500 8.672081724406845E-4 0.36461246000147973 >1k 4.955474829278011E-4 0.5812694993549832 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4228 0.10629151226575893 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.5139903563329932E-5 0.0 4 0.0 0.0 0.0 2.5139903563329932E-5 0.0 5 0.0 0.0 0.0 5.0279807126659864E-5 0.0 6 0.0 0.0 0.0 5.0279807126659864E-5 0.0 7 0.0 0.0 0.0 7.54197106899898E-5 0.0 8 0.0 0.0 0.0 7.54197106899898E-5 0.0 9 0.0 0.0 0.0 1.2569951781664964E-4 0.0 10 0.0 0.0 0.0 1.508394213799796E-4 0.0 11 0.0 2.5139903563329932E-5 0.0 1.508394213799796E-4 0.0 12 0.0 2.5139903563329932E-5 0.0 1.508394213799796E-4 0.0 13 0.0 5.0279807126659864E-5 0.0 1.508394213799796E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGACCT 65 8.056014E-4 11.687497 13 GGTATCA 1945 0.0 11.43078 1 GTATTAG 520 0.0 10.23209 1 GTATACG 105 4.102761E-5 9.953666 1 CGCTATC 260 0.0 9.861325 12 TGTTCCG 155 4.0867235E-8 9.803772 5 ACGAAAC 110 6.806899E-5 9.504455 15 GCGCGCC 110 6.8737136E-5 9.495971 11 GCCTTAT 305 0.0 9.345468 1 TGCGCTA 235 7.2759576E-12 9.293814 10 CAGTGCG 135 6.891898E-6 9.144153 9 GTATCAA 3660 0.0 9.137792 1 TATACCG 105 4.5076644E-4 9.045146 5 CACGGGT 85 0.0074518644 8.939295 4 GCGCTAT 245 1.6370905E-11 8.914585 11 CTAGACA 375 0.0 8.8648 4 TAAGACT 550 0.0 8.807237 4 TTGTGCG 270 1.8189894E-12 8.792565 7 GTGCGCT 260 7.2759576E-12 8.765402 9 GTGTTAC 305 0.0 8.722437 1 >>END_MODULE