FastQCFastQC Report
Wed 25 May 2016
SRR1294684_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294684_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3977740
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT132320.33265120394998166No Hit
GGTATCAACGCAGAGTACTTTTTTT94640.23792404732335448No Hit
TATCAACGCAGAGTACTTTTTTTTT93750.2356865959062181No Hit
ACGCAGAGTACTTTTTTTTTTTTTT44850.11275246748153475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27500.012.7397161
CGCGCGT701.0962947E-412.20882119
ACCGGTC658.069494E-411.6851678
ACCGTCT1451.4595571E-810.4763578
TGCTCGT1106.09599E-610.35730710
TGCACCG750.002614653710.1493445
GGCCGTC856.636447E-410.05242811
TCGCGCG951.6554582E-49.9938939
ACACCGT1304.271935E-69.4949156
CCGATCC1002.7742298E-49.4941999
ACAGCGT1304.2760403E-69.4941998
CCGCGCG1002.7745802E-49.4940818
AAATCGC800.0045413569.49407917
GTATTAG5750.09.4373841
CGCTATC2451.8189894E-129.30020512
GTATCAA54050.09.2999971
GCGCTAT2357.2759576E-129.29196111
TGGGCCG1651.0414806E-79.2266775
TTGTGCG2900.09.1668137
GCTATCG2503.6379788E-129.11431613
Produced by FastQC (version 0.11.5)