FastQCFastQC Report
Wed 25 May 2016
SRR1294683_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294683_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5427821
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCATTCCATTCCATTCCATTCC111440.2053125922907185No Hit
GTATCAACGCAGAGTACTTTTTTTT108540.19996974844970014No Hit
GAATGGAATGGAATGGAATGGAATG80010.14740721921375077No Hit
GTACATGGGAAGCAGTGGTATCAAC79010.14556485926857204No Hit
CCCATGTACTCTGCGTTGATACCAC77440.1426723541546414No Hit
GGTATCAACGCAGAGTACTTTTTTT62280.11474217738573177No Hit
TATCAACGCAGAGTACTTTTTTTTT60110.11074425630469391No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCCG604.1036887E-412.66346519
ATACCGA802.8804074E-511.8705766
AGGACCG803.786954E-410.6835185
TCGGAAC1355.6514182E-810.56145513
GGACCGT750.002657592510.1295586
CGGTGCA750.00265820210.1292779
AGCGTAC2400.09.8956131
TATTCCG4050.09.8481825
TCGTTGG1651.0583244E-79.2172713
CTAATAC7850.09.0732183
TGTCGCC2106.7484507E-109.04399810
TATACCG950.00183118058.9966465
TCGCTCC850.00746420358.9375989
TAGACAG9300.08.8837855
GACTCCG1401.09159755E-58.8180617
GCGACTA2502.5465852E-118.74512716
GTCCTAC3700.08.7295341
TATACTG13600.08.7283655
CTGTCGC2401.03682396E-108.7048489
TAGACTG7750.08.6999835
Produced by FastQC (version 0.11.5)