##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294683_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5427821 Sequences flagged as poor quality 0 Sequence length 25 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.346339166306333 31.0 30.0 34.0 26.0 34.0 2 30.54306488736456 31.0 30.0 34.0 26.0 34.0 3 30.477424181821767 31.0 30.0 34.0 26.0 34.0 4 34.19820163561031 37.0 35.0 37.0 30.0 37.0 5 34.47331498220004 37.0 35.0 37.0 30.0 37.0 6 34.571984042952046 37.0 35.0 37.0 31.0 37.0 7 34.50814000682779 37.0 35.0 37.0 30.0 37.0 8 34.318581250192295 37.0 35.0 37.0 30.0 37.0 9 35.56481081450549 39.0 35.0 39.0 29.0 39.0 10 35.67451892757701 39.0 35.0 39.0 29.0 39.0 11 35.89061540533485 39.0 35.0 39.0 30.0 39.0 12 35.67537046634368 39.0 35.0 39.0 29.0 39.0 13 35.55478155967192 39.0 35.0 39.0 29.0 39.0 14 36.51864753830312 39.0 36.0 41.0 27.0 41.0 15 36.505064923843285 39.0 36.0 41.0 27.0 41.0 16 36.63002869844087 39.0 36.0 41.0 29.0 41.0 17 36.605819720289226 39.0 36.0 41.0 28.0 41.0 18 36.50761880319929 39.0 36.0 41.0 27.0 41.0 19 36.803393111158236 40.0 36.0 41.0 29.0 41.0 20 36.892843739688544 40.0 36.0 41.0 29.0 41.0 21 36.9450597210188 40.0 36.0 41.0 30.0 41.0 22 36.9354381804411 40.0 36.0 41.0 30.0 41.0 23 36.91296636348177 40.0 36.0 41.0 30.0 41.0 24 36.918581692358686 40.0 36.0 41.0 30.0 41.0 25 35.83060974192038 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0134119159987023 1101 2 1.0122955601760033 1101 3 1.3519430183137047 1101 4 1.100869021132084 1101 5 0.8438712436657312 1101 6 0.8575336183379534 1101 7 0.8934690840793991 1101 8 1.0441660587098909 1101 9 1.2514268175192385 1101 10 1.2067429219208918 1101 11 0.9936426648386103 1101 12 1.1879870482417445 1101 13 1.228874951282691 1101 14 1.3335173277476216 1101 15 1.4920427815419472 1101 16 1.3801933230035814 1101 17 1.341079713380779 1101 18 1.3991705097802551 1101 19 1.2989747732581378 1101 20 1.229191838810685 1101 21 1.1581230319997786 1101 22 1.0735444689456557 1101 23 1.0978913367201386 1101 24 1.1937030979432066 1101 25 1.2213831270322189 1102 1 0.9146978123321112 1102 2 0.8613193760125668 1102 3 1.1505145539354302 1102 4 0.9443798993086787 1102 5 0.7732788318648929 1102 6 0.7268484865405043 1102 7 0.6353483154903685 1102 8 0.8037021413475216 1102 9 1.134177864972763 1102 10 1.0282795301364231 1102 11 0.8425548585345553 1102 12 1.0195761310702665 1102 13 1.0341523128562642 1102 14 1.2482568052577818 1102 15 1.3730957454811872 1102 16 1.1986855594026622 1102 17 1.2680602770383302 1102 18 1.262502512624856 1102 19 0.9862472536057183 1102 20 0.3401909072162397 1102 21 0.6252612646751459 1102 22 0.7506627978490386 1102 23 0.9023523880544104 1102 24 0.9576222516051374 1102 25 0.9548939152184275 1103 1 0.6348834964607057 1103 2 0.6317372067158082 1103 3 0.9422887720376494 1103 4 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2208 21 -0.23921858894212988 2208 22 0.1671630384694538 2208 23 0.3623860256883944 2208 24 0.4633237354562496 2208 25 0.4700557177264244 2209 1 0.12802991652262463 2209 2 -1.4148945517111038 2209 3 -0.24408335985555496 2209 4 0.10709151424023133 2209 5 0.08880252573030845 2209 6 0.11907861677120479 2209 7 0.09363915132538381 2209 8 0.2217090818614622 2209 9 -2.1233623298881596 2209 10 -0.41812239887735103 2209 11 -0.09286557763784486 2209 12 0.26835992625021987 2209 13 0.3671016889115819 2209 14 0.5199970374531162 2209 15 0.6485803853257295 2209 16 0.6081134638517582 2209 17 0.3913523280446185 2209 18 0.5929457295895517 2209 19 0.30304732026009873 2209 20 0.2829825068713063 2209 21 0.28124843970167035 2209 22 0.2611342722288512 2209 23 0.2812676506541507 2209 24 0.35038573320787947 2209 25 0.35723675098753915 2210 1 0.15486002344307437 2210 2 0.18662100226013223 2210 3 0.08437543700562244 2210 4 0.26516713791498603 2210 5 0.1337172548949468 2210 6 0.044666017515439194 2210 7 0.12763178325503333 2210 8 0.22571210314688273 2210 9 0.35531011662887835 2210 10 -1.3483661807621345 2210 11 -0.34174799552749136 2210 12 -2.122041883178028 2210 13 -0.612858082999459 2210 14 -0.2947367533107723 2210 15 -0.6319034362502975 2210 16 -0.0200192528553913 2210 17 0.21101131647936455 2210 18 0.41167379084472344 2210 19 0.30116346601116106 2210 20 0.26163044624856013 2210 21 0.2511600876526998 2210 22 0.26556033050098193 2210 23 0.1927082373585236 2210 24 0.21880596561422294 2210 25 0.14561521457520854 2211 1 -0.3062010315470829 2211 2 -0.03922228545206963 2211 3 -1.452124111949221 2211 4 -0.6201610057158504 2211 5 -0.48284287322690034 2211 6 -0.4306631588315497 2211 7 -0.43222247966388494 2211 8 -0.11205436924487344 2211 9 0.12082801196672222 2211 10 0.10420637713334457 2211 11 -0.21057534471323436 2211 12 -0.22800425180949446 2211 13 -0.3004809362940435 2211 14 -0.7978681872988531 2211 15 -0.3364275888757149 2211 16 -0.04269629071050929 2211 17 -0.1003849296205388 2211 18 0.035711028675315504 2211 19 -0.10783849282866953 2211 20 -0.09778657016447312 2211 21 -0.25716170786358106 2211 22 -0.22495004713231026 2211 23 -0.576101485640919 2211 24 -0.3403157244496384 2211 25 -0.16185451051978816 2212 1 -0.3659689746340433 2212 2 -0.33171488394544824 2212 3 -2.3900241455619984 2212 4 -1.327889182754511 2212 5 -0.572459539298265 2212 6 -0.49457662535979097 2212 7 -0.32966527268933987 2212 8 -0.3263999635811672 2212 9 -0.9161310923547745 2212 10 -0.23186907058828865 2212 11 -0.3139733868607095 2212 12 -0.06521337313816389 2212 13 -0.3827756652003842 2212 14 -0.14550420450678558 2212 15 0.06016589532267602 2212 16 -1.1814569915310713 2212 17 -1.3803728001372804 2212 18 -0.33021259861160246 2212 19 -0.1738512979569009 2212 20 -0.18189201764276675 2212 21 -0.08976919289648322 2212 22 -0.2878853039753082 2212 23 -0.11742236658336935 2212 24 -0.21332666078713203 2212 25 -0.04090337673842015 2213 1 -3.0955555254492673 2213 2 -1.4629976309496229 2213 3 -2.4692308984828166 2213 4 -1.0789132730702775 2213 5 -0.9190447347312443 2213 6 -0.6894569476352572 2213 7 -0.45838978863606883 2213 8 -0.2571414822442719 2213 9 -0.38588015664287667 2213 10 -0.38160086488904454 2213 11 -0.6798219240273653 2213 12 -0.3847595350454611 2213 13 -0.6515910543870618 2213 14 -0.3038686995755384 2213 15 -0.4640802175664476 2213 16 -1.6812848020132094 2213 17 -1.1123106198988282 2213 18 -0.41506251534691074 2213 19 -0.30492775777665315 2213 20 -0.2995053078333072 2213 21 -0.13660049803810637 2213 22 -0.2015220629018657 2213 23 -0.14847862852830218 2213 24 -0.11520634458193513 2213 25 -0.1952998853000878 2214 1 -1.0302897624011607 2214 2 -0.8284047709734139 2214 3 -6.518234821076106 2214 4 -2.186558604896394 2214 5 -1.341730295821364 2214 6 -0.869573226828841 2214 7 -0.7160655839086374 2214 8 -0.5322923683756784 2214 9 -0.32131780858144765 2214 10 -0.2919180978145093 2214 11 -0.9640437130455339 2214 12 -0.6789865274325209 2214 13 -5.345130802584549 2214 14 -3.936711944663834 2214 15 -2.2629383404490113 2214 16 -3.0402504460091464 2214 17 -3.5665575452534526 2214 18 -1.247383227784347 2214 19 -0.8282103642300882 2214 20 -0.5614639592813546 2214 21 -0.6026845092946544 2214 22 -0.5023339551498651 2214 23 -0.4547478159740237 2214 24 -0.38645587903813094 2214 25 -0.2802533918717103 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2053.0 3 249.0 4 242.0 5 662.0 6 89.0 7 73.0 8 147.0 9 371.0 10 889.0 11 2250.0 12 4776.0 13 8657.0 14 13449.0 15 19100.0 16 23725.0 17 27287.0 18 29479.0 19 30286.0 20 30926.0 21 31745.0 22 33138.0 23 34623.0 24 37670.0 25 41119.0 26 46732.0 27 53574.0 28 62691.0 29 77938.0 30 97891.0 31 124086.0 32 164465.0 33 222692.0 34 304231.0 35 422714.0 36 604204.0 37 928739.0 38 1944859.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.397335554288766 22.5897687896131 13.64399291738492 22.36890273871321 2 13.868727505656707 22.276655652791028 42.59638062848216 21.258236213070106 3 16.941521856305748 28.996961360390326 30.718850994581175 23.342665788722748 4 11.071985713566821 17.902778742958304 40.965126865274094 30.060108678200788 5 12.317060245538656 38.96647413973455 35.69819813592641 13.01826747880038 6 28.71788792594865 40.850566210826884 18.40509663488767 12.026449228336794 7 23.870519104195083 34.34909461228773 25.67639067559979 16.1039956079174 8 22.3014857345956 39.01132671228912 22.685980442147713 16.001207110967567 9 23.60367020511705 17.367529340624092 23.601660699602892 35.42713975465597 10 14.093131471048052 30.833179699713874 34.36607695938173 20.707611869856347 11 31.000389895170738 27.74035009452423 23.933056013096056 17.326203997208978 12 19.261686803492246 28.781579334987935 33.76908687719526 18.18764698432456 13 24.915153063688756 25.70523888583483 28.36284218525151 21.016765865224905 14 19.02690914051691 23.503267202704833 30.084203404453792 27.385620252324465 15 21.7624687349459 31.486727872766924 25.2229320264954 21.527871365791775 16 20.67281387017944 32.90674189119236 26.584154181273313 19.83629005735489 17 18.304848832889803 31.47636697487876 30.56269985316064 19.65608433907079 18 18.661828516647116 31.48483259526146 30.89802209529264 18.95531679279879 19 19.50021275701379 29.47929103610822 31.119197762253442 19.901298444624548 20 21.374325427704598 29.25759208961495 29.655445256890072 19.712637225790385 21 21.81176411162653 30.327399063615484 28.60948356165318 19.251353263104804 22 19.699307171275077 29.848510547294133 31.018133750235062 19.43404853119573 23 19.620040802224985 30.476286518194158 30.269307221285885 19.634365458294972 24 19.75845119361131 28.739820152102403 30.52756821459172 20.97416043969457 25 20.684257714174148 29.15190590576085 29.298771443778946 20.865064936286057 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3817.0 1 3817.0 2 3825.5 3 3834.0 4 3834.0 5 3834.0 6 7273.0 7 10712.0 8 10712.0 9 10712.0 10 18623.0 11 26534.0 12 26534.0 13 26534.0 14 46364.0 15 66194.0 16 66194.0 17 66194.0 18 104092.0 19 141990.0 20 141990.0 21 141990.0 22 199600.5 23 257211.0 24 257211.0 25 257211.0 26 328773.5 27 400336.0 28 400336.0 29 400336.0 30 473405.5 31 546475.0 32 546475.0 33 546475.0 34 604589.5 35 662704.0 36 662704.0 37 662704.0 38 698125.5 39 733547.0 40 733547.0 41 733547.0 42 701149.5 43 668752.0 44 668752.0 45 668752.0 46 635354.0 47 601956.0 48 601956.0 49 601956.0 50 541974.5 51 481993.0 52 481993.0 53 481993.0 54 416289.5 55 350586.0 56 350586.0 57 350586.0 58 291551.0 59 232516.0 60 232516.0 61 232516.0 62 184963.5 63 137411.0 64 137411.0 65 137411.0 66 100618.5 67 63826.0 68 63826.0 69 63826.0 70 44321.0 71 24816.0 72 24816.0 73 24816.0 74 16811.5 75 8807.0 76 8807.0 77 8807.0 78 5759.5 79 2712.0 80 2712.0 81 2712.0 82 1737.0 83 762.0 84 762.0 85 762.0 86 479.0 87 196.0 88 196.0 89 196.0 90 136.5 91 77.0 92 77.0 93 77.0 94 49.0 95 21.0 96 21.0 97 21.0 98 28.5 99 36.0 100 36.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10188250496838418 2 0.05243356403978686 3 0.055731388341656814 4 0.05184400885732967 5 0.048011900171357896 6 0.06803835277545077 7 0.06475895207303262 8 0.06077945459144655 9 0.06634338162588634 10 0.06744879759299358 11 0.06053994779857331 12 0.06168221096458413 13 0.061663787365132346 14 0.06367195970537716 15 0.06695136040779531 16 0.06170063456403591 17 0.06496161166700228 18 0.14449629050036836 19 0.06988071272062951 20 0.06370880690428074 21 0.061534822168969834 22 0.06781726958202933 23 0.06645392322259705 24 0.07807921447667489 25 0.07023076111021347 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 5427821.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.8904109528336 #Duplication Level Percentage of deduplicated Percentage of total 1 87.58638020469874 67.34552767810416 2 9.376552002647577 14.419338736083745 3 1.6906431708113705 3.8998274453486386 4 0.5053302279811376 1.5542019558543507 5 0.22746452772444545 0.8744920506962411 6 0.12847062389351308 0.5926895439923486 7 0.08035310574752495 0.43248683255845827 8 0.061695236313291005 0.3795017659168903 9 0.047318799265238264 0.32745257291689295 >10 0.2543959664550204 3.6076182884017745 >50 0.02347432767866432 1.2538521213810039 >100 0.01531317154773168 2.380798486342883 >500 0.001763249429031307 0.9251531373482089 >1k 6.763086453739124E-4 0.932055282288956 >5k 1.2076940094668325E-4 0.6664568565509534 >10k+ 4.8307760378673305E-5 0.4085472462145151 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTCCATTCCATTCCATTCCATTCC 11144 0.2053125922907185 No Hit GTATCAACGCAGAGTACTTTTTTTT 10854 0.19996974844970014 No Hit GAATGGAATGGAATGGAATGGAATG 8001 0.14740721921375077 No Hit GTACATGGGAAGCAGTGGTATCAAC 7901 0.14556485926857204 No Hit CCCATGTACTCTGCGTTGATACCAC 7744 0.1426723541546414 No Hit GGTATCAACGCAGAGTACTTTTTTT 6228 0.11474217738573177 No Hit TATCAACGCAGAGTACTTTTTTTTT 6011 0.11074425630469391 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.8423599451787375E-5 0.0 2 0.0 0.0 0.0 1.8423599451787375E-5 0.0 3 0.0 0.0 0.0 1.8423599451787375E-5 0.0 4 0.0 0.0 0.0 3.684719890357475E-5 0.0 5 0.0 0.0 0.0 3.684719890357475E-5 0.0 6 0.0 0.0 0.0 3.684719890357475E-5 0.0 7 0.0 0.0 0.0 3.684719890357475E-5 0.0 8 0.0 0.0 0.0 3.684719890357475E-5 0.0 9 0.0 0.0 0.0 1.1054159671072425E-4 0.0 10 0.0 1.8423599451787375E-5 0.0 1.1054159671072425E-4 0.0 11 0.0 1.8423599451787375E-5 0.0 1.1054159671072425E-4 0.0 12 0.0 1.8423599451787375E-5 0.0 1.47388795614299E-4 0.0 13 0.0 1.8423599451787375E-5 0.0 1.47388795614299E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGCCG 60 4.1036887E-4 12.663465 19 ATACCGA 80 2.8804074E-5 11.870576 6 AGGACCG 80 3.786954E-4 10.683518 5 TCGGAAC 135 5.6514182E-8 10.561455 13 GGACCGT 75 0.0026575925 10.129558 6 CGGTGCA 75 0.002658202 10.129277 9 AGCGTAC 240 0.0 9.895613 1 TATTCCG 405 0.0 9.848182 5 TCGTTGG 165 1.0583244E-7 9.21727 13 CTAATAC 785 0.0 9.073218 3 TGTCGCC 210 6.7484507E-10 9.043998 10 TATACCG 95 0.0018311805 8.996646 5 TCGCTCC 85 0.0074642035 8.937598 9 TAGACAG 930 0.0 8.883785 5 GACTCCG 140 1.09159755E-5 8.818061 7 GCGACTA 250 2.5465852E-11 8.745127 16 GTCCTAC 370 0.0 8.729534 1 TATACTG 1360 0.0 8.728365 5 CTGTCGC 240 1.03682396E-10 8.704848 9 TAGACTG 775 0.0 8.699983 5 >>END_MODULE