FastQCFastQC Report
Wed 25 May 2016
SRR1294683_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294683_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5427821
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT292680.5392219087549128No Hit
GGTATCAACGCAGAGTACTTTTTTT183960.33892053551508056No Hit
TATCAACGCAGAGTACTTTTTTTTT173980.3205337832621967No Hit
ACGCAGAGTACTTTTTTTTTTTTTT103670.1909974555166797No Hit
ATTCCATTCCATTCCATTCCATTCC103380.19046317113257788No Hit
GAATGGAATGGAATGGAATGGAATG89840.16551761747485777No Hit
CCCATGTACTCTGCGTTGATACCAC85190.15695064372977663No Hit
GTACATGGGAAGCAGTGGTATCAAC79640.14672554603403465No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT604.0205062E-412.69424256
TAGCCCG657.883923E-411.7177625
AAACCGC657.883923E-411.7177626
AGCGTCG855.4291304E-511.15591617
TGACCGT600.00578445711.1074636
ACACCGT1252.1776395E-710.6631636
GGTATCA70100.010.364531
TACACCG1303.734367E-710.2530415
CGAACCG1502.521665E-810.11441812
CGTCGGT951.6760713E-49.98179319
GGCGCCC1151.0014301E-59.9346258
TATTCCG3550.09.9229645
GGGCCGT900.00109250559.5206826
ACAGCGC1304.1280637E-69.5206818
CGCAGCG1401.0291242E-69.5206818
CAGATCG900.00109333789.519899
CGCCCGC1401.030292E-69.51988910
CTAGGAC5050.09.2181083
CGTAGAC1054.4946378E-49.04793
GCGTCGG1054.5795136E-49.0309818
Produced by FastQC (version 0.11.5)