FastQCFastQC Report
Wed 25 May 2016
SRR1294682_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294682_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1782598
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT154410.8662076362702078No Hit
GGTATCAACGCAGAGTACTTTTTTT96690.5424105715365999No Hit
TATCAACGCAGAGTACTTTTTTTTT92090.5166055386576222No Hit
ACGCAGAGTACTTTTTTTTTTTTTT40790.22882332415945714No Hit
ATTCCATTCCATTCCATTCCATTCC35680.20015729850476663No Hit
GTACTGGTTCACTATCGGTCAGTCA28670.16083267231310705No Hit
GAATGGAATGGAATGGAATGGAATG27170.15241798767865777No Hit
GTACATGGGAAGCAGTGGTATCAAC26920.15101554023958288No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26280.1474252747955512No Hit
CCCATGTACTCTGCGTTGATACCAC25390.14243256191244466No Hit
GTTAATGATAGTGTGTCGAAACACA24880.13957156913673188No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21620.12128365453119548No Hit
GGTATCAACGCAGAGTACATGGGGG18960.10636161377943877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCCG1750.013.5683619
AATCGCC2000.012.35761518
ACGTCCT701.09509885E-412.2094678
GGTATCA36150.012.19430351
AAATCGC2150.011.936583517
CACGTCC658.0491405E-411.6880237
AGCGTCG2550.011.55516717
CGTCCTT752.0812104E-411.39582359
CGCCCTA3750.011.152616
CCGTACA4900.011.05690517
ACGATTC700.001483935210.86383818
CCCGTAC4900.010.86292416
CCCCGTA4900.010.86261815
TCTATAC1605.475158E-1010.6844843
CTAGCCT909.548647E-510.5519844
GAAATCG2550.010.43692616
TTAGCAC1856.002665E-1110.2673723
CCCCGGT3350.010.20664719
GCGTCGG2900.010.16143518
GTTACCC856.6110835E-410.05598453
Produced by FastQC (version 0.11.5)