##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294682_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1782598 Sequences flagged as poor quality 0 Sequence length 25 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.52452487885659 31.0 30.0 33.0 23.0 34.0 2 29.5209037595689 31.0 30.0 34.0 23.0 34.0 3 29.52979808122751 31.0 30.0 34.0 22.0 34.0 4 32.86402374511808 35.0 33.0 37.0 22.0 37.0 5 33.55465057180587 35.0 35.0 37.0 27.0 37.0 6 33.50429373307947 37.0 35.0 37.0 27.0 37.0 7 33.42540830854741 37.0 35.0 37.0 26.0 37.0 8 33.20962381871852 37.0 33.0 37.0 25.0 37.0 9 34.07255533777105 38.0 34.0 39.0 18.0 39.0 10 34.35154084095236 38.0 34.0 39.0 23.0 39.0 11 34.51084596751483 38.0 34.0 39.0 25.0 39.0 12 34.36774584062139 38.0 34.0 39.0 23.0 39.0 13 34.127700693033425 38.0 34.0 39.0 21.0 39.0 14 34.96351897623581 39.0 34.0 41.0 18.0 41.0 15 35.00648828283214 39.0 33.0 41.0 20.0 41.0 16 35.17330940571009 39.0 34.0 41.0 23.0 41.0 17 35.23773447518734 39.0 34.0 41.0 24.0 41.0 18 35.18381261507081 39.0 34.0 41.0 23.0 41.0 19 35.5497790303815 39.0 34.0 41.0 25.0 41.0 20 35.66577714100431 39.0 34.0 41.0 25.0 41.0 21 35.76624454868681 39.0 34.0 41.0 25.0 41.0 22 35.83031507945145 39.0 34.0 41.0 26.0 41.0 23 35.87501107933477 39.0 34.0 41.0 26.0 41.0 24 35.94850213003717 39.0 34.0 41.0 27.0 41.0 25 34.94933911066881 38.0 32.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.001653229675732 1101 2 1.036617621482982 1101 3 1.2668893678385906 1101 4 0.9797770312876892 1101 5 0.7930363002899412 1101 6 0.7502150694389726 1101 7 0.6826872174455758 1101 8 0.7943270842652481 1101 9 1.1515561678596455 1101 10 1.1138684056981916 1101 11 0.8611374849171014 1101 12 1.0826432759057951 1101 13 0.9736435101071095 1101 14 1.313778065890368 1101 15 1.431286745573125 1101 16 1.3620947876073615 1101 17 1.3639824388707709 1101 18 1.2433273965623428 1101 19 1.0244084356568237 1101 20 1.012068910684178 1101 21 0.9803575822375947 1101 22 0.9392279289158054 1101 23 0.9899867271663183 1101 24 1.0263711827356659 1101 25 1.0074039005131823 1102 1 0.870056446994063 1102 2 0.916401284560628 1102 3 1.1459227500053757 1102 4 1.0127623670295094 1102 5 0.7783115929350544 1102 6 0.7019247142822991 1102 7 0.46900650286523415 1102 8 0.5011813093068511 1102 9 0.9408666205883947 1102 10 0.8739694624919707 1102 11 0.6760730532104446 1102 12 0.9280644288493178 1102 13 0.893385143230411 1102 14 1.1725517299636508 1102 15 1.2902140216622797 1102 16 1.0681560617336956 1102 17 1.0732912559160184 1102 18 1.099277373093841 1102 19 0.7998838506226136 1102 20 0.14616900029118796 1102 21 0.47980785034745566 1102 22 0.40595634856107665 1102 23 0.5226267914557923 1102 24 0.7921167699460412 1102 25 0.8107670080273692 1103 1 0.805887744659973 1103 2 0.7591735752947173 1103 3 1.0018361705032461 1103 4 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0.31061213390018594 2207 6 0.33495815405117213 2207 7 0.268333591741154 2207 8 0.2350276762861938 2207 9 -1.0997346819243674 2207 10 -0.06454215709467803 2207 11 0.32043791011427203 2207 12 0.5079452201486845 2207 13 0.7482395049803259 2207 14 0.8750054412642001 2207 15 0.8128439030588339 2207 16 0.8119689916341954 2207 17 0.7155720117069819 2207 18 0.7779616388365724 2207 19 -0.5351103523617056 2207 20 0.09308965515220535 2207 21 -0.28355084100651595 2207 22 0.19958965167640486 2207 23 -0.5536258212363094 2207 24 -1.5466552445908874 2207 25 -0.161950030356671 2208 1 0.6423452528316353 2208 2 0.3519725072792568 2208 3 0.7031160833825716 2208 4 0.6442561063785064 2208 5 0.5947373303627614 2208 6 0.46977069502989366 2208 7 0.5391504682293302 2208 8 0.6423183593328474 2208 9 -0.007551931730645833 2208 10 0.17494376191097416 2208 11 0.3878273185462433 2208 12 0.49360666768180295 2208 13 0.6052249455741219 2208 14 0.849654788898647 2208 15 0.8724631185888683 2208 16 0.5999843823790414 2208 17 0.7326514719956592 2208 18 0.7979046686110465 2208 19 -1.1666053815840414 2208 20 0.0561256725067949 2208 21 0.014865851018591059 2208 22 0.4843620374300812 2208 23 0.5777743098955952 2208 24 0.6914523776864812 2208 25 0.6325154502668582 2209 1 0.17394648999194473 2209 2 -1.3652631895918717 2209 3 -0.3258023851703733 2209 4 0.08978354434371028 2209 5 0.058292137117952336 2209 6 0.13195890695816104 2209 7 0.11420542409553747 2209 8 0.3364712088905861 2209 9 -1.8935032139317087 2209 10 -0.23600698226062633 2209 11 -0.01307380393693336 2209 12 0.3181743225804823 2209 13 0.5133425803351273 2209 14 0.5093179517125108 2209 15 0.7667563169741669 2209 16 0.7401647298538379 2209 17 0.41756727807139526 2209 18 0.6451590274182806 2209 19 0.36339206661909174 2209 20 0.31061975571996214 2209 21 0.5197107294393035 2209 22 0.4060555389565508 2209 23 0.40288091658991476 2209 24 0.4896681182763487 2209 25 0.5177486937236537 2210 1 0.12788240398018402 2210 2 0.14079946188381243 2210 3 0.02272789486616844 2210 4 0.21131925296678133 2210 5 0.15091197750693652 2210 6 0.03905819950536937 2210 7 0.10471173982938353 2210 8 0.17586726920150397 2210 9 0.24242300561271435 2210 10 -1.510710599556603 2210 11 -0.46246306907694645 2210 12 -2.1728885176499446 2210 13 -0.7821962389661721 2210 14 -0.3927802460571641 2210 15 -0.5434609019888939 2210 16 -0.08317426612190815 2210 17 0.040979057877656544 2210 18 0.40426266887486406 2210 19 0.24023352018165411 2210 20 0.3389926142973607 2210 21 0.16579731501693118 2210 22 0.2570640933216879 2210 23 0.19598412013548483 2210 24 0.18591861847642832 2210 25 0.1251349689884762 2211 1 -0.45642941219757915 2211 2 -0.26393458282751325 2211 3 -1.5625647032864123 2211 4 -0.9119022344353347 2211 5 -0.4400072689192669 2211 6 -0.4961555519992089 2211 7 -0.4257587704423216 2211 8 -0.10942397652144109 2211 9 -0.07558134921072934 2211 10 -0.009800955380839582 2211 11 -0.33315348440809345 2211 12 -0.38303714966736635 2211 13 -0.4711179468143243 2211 14 -1.0615485883841558 2211 15 -0.6891240658627922 2211 16 -0.4382297833586293 2211 17 -0.2538176043259881 2211 18 -0.23883114916925052 2211 19 -0.1429444189860618 2211 20 -0.10228083345152328 2211 21 -0.27875225829151873 2211 22 -0.15992837364521506 2211 23 -0.5344620464027017 2211 24 -0.4880663154573881 2211 25 -0.2745718242656068 2212 1 -0.24053353382350906 2212 2 -0.16625593170219233 2212 3 -2.0483896950269305 2212 4 -1.0328789977620794 2212 5 -0.24650298847945606 2212 6 -0.07431400651989861 2212 7 -0.032295062891705584 2212 8 -0.12887519921443413 2212 9 -0.3629608622201346 2212 10 -0.0020225529093735872 2212 11 0.09149222732732909 2212 12 0.2452844658291582 2212 13 -0.12548098143459185 2212 14 0.21810410768433286 2212 15 0.4223116927632944 2212 16 -0.7605697957623363 2212 17 -1.0291278420251615 2212 18 0.03934527334521931 2212 19 0.11424712562382666 2212 20 0.17242312936052429 2212 21 0.26933472998292274 2212 22 -0.1912935354441032 2212 23 0.2916303786388852 2212 24 0.15007799651335318 2212 25 0.13496976004775973 2213 1 -3.0170545920824416 2213 2 -1.4490950196489685 2213 3 -2.47866479037571 2213 4 -1.1605542199877945 2213 5 -0.9874881445576165 2213 6 -0.6988787906125538 2213 7 -0.5376940415918412 2213 8 -0.329880108689359 2213 9 -0.5959785035490697 2213 10 -0.29912009443523857 2213 11 -0.6576156002321412 2213 12 -0.4906974588786355 2213 13 -0.8102278092989366 2213 14 -0.26087007447323884 2213 15 -0.587349212920607 2213 16 -1.7597027184582785 2213 17 -1.1915027166556342 2213 18 -0.4227801785583125 2213 19 -0.1924519594864762 2213 20 -0.1256972406717196 2213 21 -0.04933520230692068 2213 22 -0.09949764934799532 2213 23 -0.14282452187651984 2213 24 -0.03933669658289318 2213 25 -0.026753999823633023 2214 1 -0.7868640619820866 2214 2 -0.768168550853197 2214 3 -5.938935153331833 2214 4 -1.9183975415524444 2214 5 -1.3460012274913709 2214 6 -0.6805963573528331 2214 7 -0.49776039796190474 2214 8 -0.5509306272620478 2214 9 -0.45312241823058486 2214 10 -0.3275692862937092 2214 11 -1.0258629822337042 2214 12 -0.6004805473696351 2214 13 -5.015566721911128 2214 14 -3.623833944132137 2214 15 -2.1170272647041912 2214 16 -2.7766185658276115 2214 17 -3.3223788810425923 2214 18 -1.2489794790481383 2214 19 -0.4947652736621251 2214 20 -0.26861581914694455 2214 21 -0.3722414749671472 2214 22 -0.29454636646506316 2214 23 -0.25611893385207196 2214 24 -0.01718072054996611 2214 25 0.14059354958383352 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 724.0 3 91.0 4 95.0 5 192.0 6 26.0 7 33.0 8 51.0 9 125.0 10 423.0 11 1250.0 12 2858.0 13 5753.0 14 9889.0 15 15110.0 16 19894.0 17 23142.0 18 23876.0 19 23264.0 20 21846.0 21 19943.0 22 18467.0 23 17222.0 24 16652.0 25 16310.0 26 16803.0 27 17855.0 28 20595.0 29 24907.0 30 30706.0 31 38674.0 32 51834.0 33 69564.0 34 95736.0 35 132577.0 36 190040.0 37 291313.0 38 564758.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.16641218459058 23.098923425697503 13.607481379942396 20.127183009769528 2 14.415702360585364 24.62179893039484 40.867662180791115 20.094836528228686 3 17.086634106583844 32.27641231370617 30.01520605637748 20.621747523332512 4 10.833450267451715 20.51553914112358 38.318730600629756 30.332279990794948 5 11.831305284899646 41.625039288761165 32.87717210722464 13.666483319114544 6 26.37263625314722 42.31921564444644 18.13308557907412 13.17506252333222 7 22.320252742478182 38.42618633021559 23.600356569878603 15.653204357427622 8 22.13302018440909 39.610120607990915 21.859033586793856 16.39782562080614 9 23.524871306915465 20.24582203584881 22.259665313775354 33.969641343460374 10 15.185064180507043 33.40501924909985 30.778013924020787 20.63190264637231 11 28.7189213978628 31.63990163295801 22.051584880305317 17.58959208887387 12 18.819493093352012 33.058055360959926 31.044383521043272 17.07806802464479 13 24.33414315416983 29.25576941212396 27.042252888635414 19.367834545070796 14 19.070348373769242 27.409875268044594 28.812520348935116 24.707256009251047 15 22.052259689576523 32.972824894056444 25.05505584737166 19.919859568995378 16 19.594129973386952 34.8513042975497 26.15477106660447 19.399794662458874 17 17.37710697218547 33.213914619174616 29.447958832540795 19.96101957609912 18 17.863236387150273 32.01999335951699 30.97313413382427 19.14363611950847 19 18.941079781106637 30.59746570528438 31.65162542019307 18.809829093415907 20 21.712312657212447 28.97289644762317 30.18243239829642 19.13235849686796 21 22.43190007314213 30.51183212898612 29.040906266366463 18.015361531505285 22 20.432491239809718 29.807999982036137 31.023985125921065 18.735523652233077 23 19.954608428932943 30.546349251258007 30.79076540315754 18.70827691665151 24 19.7846506763141 28.741538705108187 30.950219486986015 20.523591131591697 25 20.628867547769108 29.235812138016055 30.166970840310803 19.968349473904034 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3261.0 1 3261.0 2 3849.5 3 4438.0 4 4438.0 5 4438.0 6 6928.5 7 9419.0 8 9419.0 9 9419.0 10 12750.0 11 16081.0 12 16081.0 13 16081.0 14 22377.5 15 28674.0 16 28674.0 17 28674.0 18 41528.5 19 54383.0 20 54383.0 21 54383.0 22 73200.5 23 92018.0 24 92018.0 25 92018.0 26 117134.5 27 142251.0 28 142251.0 29 142251.0 30 164221.0 31 186191.0 32 186191.0 33 186191.0 34 201545.0 35 216899.0 36 216899.0 37 216899.0 38 220308.0 39 223717.0 40 223717.0 41 223717.0 42 213517.0 43 203317.0 44 203317.0 45 203317.0 46 200408.0 47 197499.0 48 197499.0 49 197499.0 50 177451.5 51 157404.0 52 157404.0 53 157404.0 54 131851.5 55 106299.0 56 106299.0 57 106299.0 58 85541.0 59 64783.0 60 64783.0 61 64783.0 62 53556.0 63 42329.0 64 42329.0 65 42329.0 66 30918.5 67 19508.0 68 19508.0 69 19508.0 70 14204.5 71 8901.0 72 8901.0 73 8901.0 74 6203.5 75 3506.0 76 3506.0 77 3506.0 78 2358.5 79 1211.0 80 1211.0 81 1211.0 82 778.5 83 346.0 84 346.0 85 346.0 86 226.0 87 106.0 88 106.0 89 106.0 90 70.0 91 34.0 92 34.0 93 34.0 94 23.5 95 13.0 96 13.0 97 13.0 98 11.5 99 10.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10417379577448196 2 0.0564344849483731 3 0.06008084828996779 4 0.054302764840979297 5 0.05149786996282953 6 0.07085164462206285 7 0.06619551912433426 8 0.06305403686080653 9 0.06888821820735802 10 0.06821504343660209 11 0.06210037260223561 12 0.06316623265593252 13 0.06361501583643649 14 0.06608332332920827 15 0.06871992451466903 16 0.06400770111937745 17 0.0668125959975272 18 0.1462472189467283 19 0.07146872149525581 20 0.0656906380462673 21 0.06367111373399947 22 0.06978578456836594 23 0.06787845605122411 24 0.0784809586906302 25 0.07118823200744082 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1782598.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.3697265816837 #Duplication Level Percentage of deduplicated Percentage of total 1 86.98421618829322 61.21055510092259 2 9.380178711474194 13.201612224275383 3 1.984067809893282 4.188549279051307 4 0.6402110044894317 1.8020589336202555 5 0.2734298367223502 0.9620591424713101 6 0.1586471186534079 0.6698372617567347 7 0.10478227284977498 0.5161449923732232 8 0.06605217686666445 0.37184589009857527 9 0.05380665141832165 0.34077234137249324 >10 0.2886063746008355 3.8887811246054707 >50 0.028818290985614604 1.4203125014893998 >100 0.03074552278158191 4.422121101498639 >500 0.0035409225342715378 1.7441029932895546 >1k 0.0026556919007036535 3.3177484890207753 >5k 1.609510242850699E-4 1.0690686507994094 >10k+ 8.047551214253496E-5 0.8744299733548936 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 15441 0.8662076362702078 No Hit GGTATCAACGCAGAGTACTTTTTTT 9669 0.5424105715365999 No Hit TATCAACGCAGAGTACTTTTTTTTT 9209 0.5166055386576222 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 4079 0.22882332415945714 No Hit ATTCCATTCCATTCCATTCCATTCC 3568 0.20015729850476663 No Hit GTACTGGTTCACTATCGGTCAGTCA 2867 0.16083267231310705 No Hit GAATGGAATGGAATGGAATGGAATG 2717 0.15241798767865777 No Hit GTACATGGGAAGCAGTGGTATCAAC 2692 0.15101554023958288 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2628 0.1474252747955512 No Hit CCCATGTACTCTGCGTTGATACCAC 2539 0.14243256191244466 No Hit GTTAATGATAGTGTGTCGAAACACA 2488 0.13957156913673188 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2162 0.12128365453119548 No Hit GGTATCAACGCAGAGTACATGGGGG 1896 0.10636161377943877 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.6097897562995135E-5 0.0 2 0.0 0.0 0.0 5.6097897562995135E-5 0.0 3 0.0 0.0 0.0 5.6097897562995135E-5 0.0 4 0.0 0.0 0.0 5.6097897562995135E-5 0.0 5 0.0 0.0 0.0 5.6097897562995135E-5 0.0 6 0.0 0.0 0.0 5.6097897562995135E-5 0.0 7 0.0 0.0 0.0 5.6097897562995135E-5 0.0 8 0.0 0.0 0.0 5.6097897562995135E-5 0.0 9 0.0 0.0 0.0 5.6097897562995135E-5 0.0 10 0.0 0.0 0.0 5.6097897562995135E-5 0.0 11 0.0 0.0 0.0 5.6097897562995135E-5 0.0 12 0.0 0.0 0.0 5.6097897562995135E-5 0.0 13 0.0 0.0 0.0 5.6097897562995135E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGCCG 175 0.0 13.56836 19 AATCGCC 200 0.0 12.357615 18 ACGTCCT 70 1.09509885E-4 12.209467 8 GGTATCA 3615 0.0 12.1943035 1 AAATCGC 215 0.0 11.9365835 17 CACGTCC 65 8.0491405E-4 11.688023 7 AGCGTCG 255 0.0 11.555167 17 CGTCCTT 75 2.0812104E-4 11.3958235 9 CGCCCTA 375 0.0 11.1526 16 CCGTACA 490 0.0 11.056905 17 ACGATTC 70 0.0014839352 10.863838 18 CCCGTAC 490 0.0 10.862924 16 CCCCGTA 490 0.0 10.862618 15 TCTATAC 160 5.475158E-10 10.684484 3 CTAGCCT 90 9.548647E-5 10.551984 4 GAAATCG 255 0.0 10.436926 16 TTAGCAC 185 6.002665E-11 10.267372 3 CCCCGGT 335 0.0 10.206647 19 GCGTCGG 290 0.0 10.161435 18 GTTACCC 85 6.6110835E-4 10.0559845 3 >>END_MODULE