FastQCFastQC Report
Wed 25 May 2016
SRR1294682_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294682_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1782598
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT432772.4277487128337403No Hit
GGTATCAACGCAGAGTACTTTTTTT299511.6801881299092671No Hit
TATCAACGCAGAGTACTTTTTTTTT272851.5306311350063222No Hit
ACGCAGAGTACTTTTTTTTTTTTTT147740.8287903385956901No Hit
GTACTTTTTTTTTTTTTTTTTTTTT83400.4678564656753794No Hit
GAGTACTTTTTTTTTTTTTTTTTTT71110.3989121495704584No Hit
GCAGAGTACTTTTTTTTTTTTTTTT54800.3074164786452133No Hit
ATCAACGCAGAGTACTTTTTTTTTT44750.25103809159440327No Hit
ATTCCATTCCATTCCATTCCATTCC32700.18344012503099408No Hit
GTGGTATCAACGCAGAGTACTTTTT29810.1672278326352885No Hit
GAATGGAATGGAATGGAATGGAATG29610.1661058746840286No Hit
CCCATGTACTCTGCGTTGATACCAC29030.1628521966253749No Hit
GTACTGGTTCACTATCGGTCAGTCA26880.1507911486493309No Hit
GTACATGGGAAGCAGTGGTATCAAC25460.1428252471953856No Hit
GTTAATGATAGTGTGTCGAAACACA24000.1346349541511883No Hit
GGTATCAACGCAGAGTACATGGGGG19430.10899821496489955No Hit
ATACAGGGTGACAGCCCCGTACACA18230.10226646725734012No Hit
ACTTAGATGTTTCAGTTCCCCCGGT18140.10176158617927317No Hit
CAACGCAGAGTACTTTTTTTTTTTT17920.10052743243288728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60200.014.2608441
GCCCGCC400.005325894314.23121911
TAGGACC707.0721126E-613.6066384
ATCGCCG1700.013.3843219
CCCGGGT500.00152334713.2724116
AGGACCG655.3223685E-513.1879725
AAATCGC1900.012.97303417
AATCGCC1900.012.97303418
GAAATCG2000.011.37635116
CGCGCGA855.438979E-511.15297413
CCCCGTA4100.011.09856915
CGGGTTC600.00596775111.06034218
CCCGTAC4050.011.001821516
CCGTACA4150.010.96515917
ACAGGGT3950.010.5787263
GACAGCT1002.3312765E-510.4771117
GCCCCGT4100.010.40490814
GTCATAT1659.1677066E-1010.3715241
GTATCAA128450.010.3324981
CGTACCA750.002593397910.15876410
Produced by FastQC (version 0.11.5)