##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294682_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1782598
Sequences flagged as poor quality	0
Sequence length	25
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.052847585378196	31.0	31.0	34.0	28.0	34.0
2	31.194024115364204	31.0	31.0	34.0	28.0	34.0
3	31.235366582931206	31.0	31.0	34.0	28.0	34.0
4	34.75146106974203	37.0	35.0	37.0	32.0	37.0
5	35.013379348568776	37.0	35.0	37.0	32.0	37.0
6	34.450554191130024	37.0	35.0	37.0	30.0	37.0
7	34.293090197565576	37.0	35.0	37.0	30.0	37.0
8	34.31573018706405	37.0	35.0	37.0	30.0	37.0
9	35.640151621397536	39.0	35.0	39.0	29.0	39.0
10	35.47779028137583	39.0	35.0	39.0	29.0	39.0
11	35.60552968195858	39.0	35.0	39.0	29.0	39.0
12	35.59771692776498	39.0	35.0	39.0	29.0	39.0
13	35.385276433609825	39.0	35.0	39.0	27.0	39.0
14	36.32560173409821	39.0	36.0	41.0	27.0	41.0
15	36.371581253877764	39.0	36.0	41.0	27.0	41.0
16	36.44440473959917	39.0	36.0	41.0	28.0	41.0
17	36.58988790518109	39.0	36.0	41.0	29.0	41.0
18	36.64754756821224	39.0	36.0	41.0	29.0	41.0
19	36.73377732949324	40.0	36.0	41.0	29.0	41.0
20	36.808847536012046	40.0	36.0	41.0	29.0	41.0
21	36.65901117357924	40.0	36.0	41.0	28.0	41.0
22	36.66213021668374	40.0	36.0	41.0	28.0	41.0
23	36.681697724332686	39.0	36.0	41.0	29.0	41.0
24	36.60634085755734	39.0	36.0	41.0	28.0	41.0
25	35.49636261232202	39.0	34.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.4664518752483353
1101	2	0.5301000917444405
1101	3	0.3990043876084002
1101	4	0.27170146233390824
1101	5	0.36313868069101574
1101	6	0.42563826351548784
1101	7	0.6497060221291449
1101	8	0.5834214775098303
1101	9	0.6848152656274351
1101	10	0.774951145749533
1101	11	0.8386032764801783
1101	12	0.9291218700281689
1101	13	0.8022945326701176
1101	14	1.045950124155489
1101	15	1.0393363496156667
1101	16	1.151734708928089
1101	17	1.0832301124280477
1101	18	0.9953213954261173
1101	19	1.1030503295908574
1101	20	0.9890958554529874
1101	21	1.3202371162099453
1101	22	1.2998179099788487
1101	23	1.3138037856112632
1101	24	1.4204328305503324
1101	25	1.369427683800808
1102	1	0.46520946695328647
1102	2	0.48085428407237174
1102	3	0.3441257837709415
1102	4	0.3180851794602262
1102	5	0.3520480353783171
1102	6	0.4361848662719723
1102	7	0.4476575063341315
1102	8	0.5056457937230121
1102	9	0.6653698334514075
1102	10	0.8330157765828758
1102	11	0.7070822330563402
1102	12	0.9059378342991522
1102	13	0.7405129888693622
1102	14	0.9527915481957692
1102	15	0.9806682938032978
1102	16	0.9512258691255084
1102	17	0.9230728734617983
1102	18	0.7663726421014161
1102	19	1.030262769319478
1102	20	0.9359204959582001
1102	21	1.1008752461258808
1102	22	1.055292115961187
1102	23	1.0633155696441534
1102	24	1.0931432292304066
1102	25	0.9746689826757446
1103	1	0.3074079737086066
1103	2	0.28559070908879036
1103	3	0.1455409769519136
1103	4	0.1754734919868568
1103	5	0.18283942130570097
1103	6	0.26216237337747117
1103	7	0.21721239481893662
1103	8	0.31259297465763325
1103	9	0.2968563699197375
1103	10	0.43222239366223647
1103	11	0.39389181131105033
1103	12	0.4687566324748573
1103	13	0.5317514270518728
1103	14	0.5929474261766714
1103	15	0.5796753873483667
1103	16	0.6188335485490271
1103	17	0.5426781789947341
1103	18	0.5999503258423786
1103	19	0.6287878421323825
1103	20	0.5665762410406003
1103	21	0.8042639116421668
1103	22	0.5353610333236318
1103	23	0.6149278252761619
1103	24	0.8185991976385338
1103	25	0.756968942313982
1104	1	0.1385846695293651
1104	2	0.09438119166670589
1104	3	0.006398550083684285
1104	4	0.036388271288615215
1104	5	0.15253647076587384
1104	6	-0.01944903669144793
1104	7	0.14224370905522932
1104	8	0.15743018800821318
1104	9	-0.14307802899257638
1104	10	0.3150169536713321
1104	11	0.3840904290274878
1104	12	0.24948292622046608
1104	13	-0.09639214850221123
1104	14	0.21611932931364208
1104	15	0.3748199171575237
1104	16	0.41824046445911733
1104	17	0.49865557448556075
1104	18	0.3589912696669728
1104	19	0.4868989926677898
1104	20	0.3117394242726661
1104	21	0.5971491486950598
1104	22	0.589140272958879
1104	23	0.56094678473454
1104	24	0.5364609994644312
1104	25	0.6124773875941685
1105	1	-0.0898993798386627
1105	2	-0.06551821228354626
1105	3	-0.041414421416661185
1105	4	-0.1124443802565267
1105	5	-0.07375960753817878
1105	6	-0.20096894609356042
1105	7	-0.2345692435938247
1105	8	-0.35441627454789426
1105	9	-0.01541572116224188
1105	10	0.006141768038524731
1105	11	0.030615718028180083
1105	12	-0.3801147803459983
1105	13	-0.5711331702279736
1105	14	-1.004260761493704
1105	15	-0.317609476047501
1105	16	-0.21329106990293667
1105	17	-0.12608059477944522
1105	18	-1.0009091117573945
1105	19	-0.8737744660475499
1105	20	-0.8242696397051077
1105	21	-0.14137111608871322
1105	22	0.05773141159752271
1105	23	0.17590960929958044
1105	24	0.20087371556475375
1105	25	0.06374611407806441
1106	1	-0.4232780001251619
1106	2	-0.44714642261435245
1106	3	-0.5356728663457488
1106	4	-0.46601524662560223
1106	5	-0.3434250219909245
1106	6	-0.6030793181110496
1106	7	-0.4854184234055765
1106	8	-0.42781139589715167
1106	9	-0.41397848334205634
1106	10	-1.5597572748840136
1106	11	-1.0644649323003748
1106	12	-1.7451280183750129
1106	13	-0.783440779780868
1106	14	-0.9009410498430555
1106	15	-0.864997427423944
1106	16	-0.9181397798378299
1106	17	-1.1679419557927986
1106	18	-0.6302664483624625
1106	19	-0.36929738599207695
1106	20	-0.4975412799471357
1106	21	-0.24225086953783403
1106	22	-0.29334432163582846
1106	23	-0.7333606497892546
1106	24	-0.301423657950167
1106	25	-0.985626948372051
1107	1	0.1455833667107953
1107	2	0.13552370683432713
1107	3	0.10143855399197221
1107	4	0.06745297370574832
1107	5	0.0817214136163571
1107	6	0.07918806484424579
1107	7	-0.01699470005054593
1107	8	0.11097534521895369
1107	9	0.21894776269933658
1107	10	0.19474289163963476
1107	11	0.3179341152260591
1107	12	-0.2224750208640529
1107	13	0.014591530545665421
1107	14	0.05674715836818223
1107	15	0.144033897787736
1107	16	0.03367801959718264
1107	17	0.2134955764196249
1107	18	0.18746910677733553
1107	19	0.20195615891442742
1107	20	0.10038969453181323
1107	21	0.4931476034488469
1107	22	0.3824933719254062
1107	23	0.4041449491774429
1107	24	0.48866385368610565
1107	25	0.45806218448614544
1108	1	0.08837687087599377
1108	2	0.1924459147021409
1108	3	0.13324897215279918
1108	4	0.05609637209713725
1108	5	0.07738376493144017
1108	6	-0.014677211237369647
1108	7	0.08327102008871634
1108	8	0.06625608645605752
1108	9	0.11754410558312856
1108	10	-0.9412198502279097
1108	11	-0.03843360941423413
1108	12	0.11486221132054908
1108	13	0.18719954878531553
1108	14	0.2907711913396227
1108	15	0.1020377895730249
1108	16	0.07341152220001845
1108	17	0.10307470696636756
1108	18	0.2053815255136513
1108	19	0.1473592083562778
1108	20	0.15766206101127267
1108	21	0.4627291835356502
1108	22	0.3460772688688607
1108	23	0.34007738583779457
1108	24	0.47400524066750904
1108	25	0.326833178791361
1109	1	-0.06589939051006866
1109	2	-0.01213717777088874
1109	3	-0.03340519865508895
1109	4	0.014808453334921978
1109	5	-0.05078940876992277
1109	6	-0.07941731050068768
1109	7	-0.14466265840333392
1109	8	-0.049102715327599356
1109	9	0.06866794531901377
1109	10	-0.34060618805398235
1109	11	0.043860598755223634
1109	12	0.07391947753117023
1109	13	0.2598721294584223
1109	14	0.260584162986099
1109	15	-3.3433085531043787
1109	16	-1.4957358087002959
1109	17	-1.4326920636049394
1109	18	-0.7348909997156454
1109	19	-0.2867785065081776
1109	20	-0.17930494204601644
1109	21	0.36060609512639985
1109	22	0.38038667053248787
1109	23	0.21333756064331055
1109	24	0.32057252850436413
1109	25	0.3686078060026148
1110	1	-0.0766632970954646
1110	2	-0.11313809581965018
1110	3	-0.17703879195882521
1110	4	-0.07583893320464341
1110	5	-0.04198123727550751
1110	6	-0.8980580026313518
1110	7	-0.9118385100805426
1110	8	-0.8476315537741854
1110	9	-0.3370637699892356
1110	10	0.02601628607087747
1110	11	0.030001835294754642
1110	12	0.087144807204659
1110	13	0.15329457816650205
1110	14	0.15799651646729984
1110	15	0.028537433129521617
1110	16	0.1538457411010299
1110	17	-0.024576366956843287
1110	18	-0.21157673117281206
1110	19	0.20361623460141232
1110	20	0.06453463307016705
1110	21	-1.120967733004541
1110	22	-1.0517116434216547
1110	23	-1.1289493405152058
1110	24	-1.1553116477636465
1110	25	-0.7901790524636567
1111	1	-0.40928095660861175
1111	2	-0.2811097200369552
1111	3	-0.2215991252923395
1111	4	-0.1730390841213918
1111	5	-0.23925112188931053
1111	6	-0.3105660954433631
1111	7	-0.4228107441546314
1111	8	-0.4018725808381234
1111	9	-0.40207165841245995
1111	10	-0.32336975148263036
1111	11	-0.5748681278186609
1111	12	-0.6859015267045407
1111	13	-0.36383803231927914
1111	14	-0.5158984468384276
1111	15	-0.6486871442597604
1111	16	-0.7039333855532846
1111	17	-0.7413807210015122
1111	18	-1.2136814437073653
1111	19	-0.5664494220536938
1111	20	-0.5048161369075643
1111	21	-0.13691900313997252
1111	22	-0.3414312720977719
1111	23	-1.4759548972067549
1111	24	-1.1983663021301325
1111	25	-1.7800765217458547
1112	1	-0.257974756924213
1112	2	-0.3724459084881069
1112	3	-0.353416619891167
1112	4	-0.18454090729576222
1112	5	-0.18684024495284035
1112	6	-0.14824915582059361
1112	7	-0.25028126058697353
1112	8	-0.13071541860086455
1112	9	-0.5044004776433084
1112	10	-0.1840545079915259
1112	11	-0.10229514512321458
1112	12	-0.42095952058866004
1112	13	-0.12277123385508304
1112	14	-0.7449072387885209
1112	15	-0.26187142900185734
1112	16	-0.2239825458826772
1112	17	-0.35034715052177745
1112	18	-0.17778481932883494
1112	19	-1.2929433626363362
1112	20	-0.9382168734581242
1112	21	-0.8858170376183239
1112	22	-0.9317965310420249
1112	23	-0.5386542836870305
1112	24	-1.1420053997851127
1112	25	-0.9105211563828988
1113	1	-0.019970649381271244
1113	2	-0.12092891819684226
1113	3	0.010871094821307992
1113	4	0.10345386492230801
1113	5	-0.03724166392222372
1113	6	0.033836355755205716
1113	7	-0.05174223892902319
1113	8	-0.09101694959414175
1113	9	-0.40488591453651424
1113	10	0.10811782210556231
1113	11	0.4248006353099427
1113	12	0.07138551301851237
1113	13	0.07194030460645706
1113	14	-0.03145503565185237
1113	15	0.4684310355239063
1113	16	-0.20311809479977683
1113	17	-0.2157784425307412
1113	18	0.1771442322021599
1113	19	-0.1958093073712135
1113	20	-0.004147605347597505
1113	21	-9.95895930796933
1113	22	-2.490795784216033
1113	23	-1.592355739997707
1113	24	-0.39032504130864254
1113	25	-1.5794905365173264
1201	1	0.4660267561447924
1201	2	0.5133344346006581
1201	3	0.4775372227496568
1201	4	0.2784228257387511
1201	5	0.42980741715857107
1201	6	0.5712244581235666
1201	7	0.5229561854634284
1201	8	0.5751807902639428
1201	9	0.7996162317744862
1201	10	0.793583313335013
1201	11	0.8165275901612077
1201	12	0.8577479362006031
1201	13	0.7942869230021685
1201	14	1.1483037577359383
1201	15	1.0373474509477134
1201	16	0.9902000685604904
1201	17	0.9631661825780995
1201	18	0.8764164640805134
1201	19	1.1100485963575935
1201	20	1.0069286299400773
1201	21	1.2720227244344287
1201	22	1.18452010162801
1201	23	1.2374305627153106
1201	24	1.229055441055202
1201	25	1.2418472270407506
1202	1	0.32481350250927576
1202	2	0.35483927317539354
1202	3	0.35039600790242176
1202	4	0.1896598019139546
1202	5	0.2944091294841513
1202	6	0.23945745870390311
1202	7	0.33393212074193457
1202	8	0.3050691982686047
1202	9	0.34794306011210097
1202	10	0.5600331172998096
1202	11	0.423434350708753
1202	12	0.47031047955432825
1202	13	0.4753493067743406
1202	14	0.59612165149845
1202	15	0.6199625787479732
1202	16	0.5864784761800408
1202	17	0.5167612827824826
1202	18	0.5038479358651244
1202	19	0.6204270636044242
1202	20	0.5815931877398128
1202	21	0.8278050436834192
1202	22	0.7636622389938665
1202	23	0.8035319875284941
1202	24	0.9536421115465146
1202	25	0.9304407092962137
1203	1	0.17158077890400492
1203	2	0.1464207955642749
1203	3	0.23318606884895132
1203	4	0.10647980929743284
1203	5	0.1417576247832102
1203	6	0.13122619559067772
1203	7	0.24983466760358652
1203	8	0.18333423006004068
1203	9	0.38925304484153145
1203	10	0.3871162891469524
1203	11	0.3385061469212829
1203	12	0.18229794428464885
1203	13	0.39427399924989714
1203	14	0.42507127525205135
1203	15	0.4049255102773941
1203	16	0.5004113075595527
1203	17	0.4977046424046705
1203	18	0.2986614592770067
1203	19	0.5201798528814763
1203	20	0.5363732008513935
1203	21	0.6554326484282882
1203	22	0.5275382053381747
1203	23	0.6245189298099305
1203	24	0.7918363215166622
1203	25	0.6070494073474748
1204	1	0.1590953626961742
1204	2	0.1360939654535045
1204	3	0.12245039080626441
1204	4	0.024792615728721046
1204	5	0.10143030348450566
1204	6	0.1878580512933965
1204	7	0.06386722015937352
1204	8	0.16309623102234383
1204	9	-0.2765598526971971
1204	10	0.15809569147158697
1204	11	0.1496644578474644
1204	12	0.266862020278289
1204	13	0.1223375727001823
1204	14	0.18590183707069485
1204	15	0.3398885312204811
1204	16	0.3793427255768549
1204	17	0.2792086704097727
1204	18	0.3110219658660611
1204	19	0.3117363323632887
1204	20	0.28948920729282435
1204	21	0.43955106380320785
1204	22	0.5762136088120897
1204	23	0.4737200100008536
1204	24	0.4846737979937714
1204	25	0.43251660365204714
1205	1	0.04925718239328347
1205	2	0.012415794070420816
1205	3	0.08190390442696582
1205	4	-0.03534427931747075
1205	5	-0.04923204864887509
1205	6	-0.03174791763829177
1205	7	-0.017201759447154075
1205	8	-0.01826394782543872
1205	9	0.043023640296446786
1205	10	-0.05063855684765883
1205	11	-0.18280194644702874
1205	12	-0.21749550309210264
1205	13	0.1542304700920596
1205	14	-0.4872966124071425
1205	15	-0.02819200250012699
1205	16	0.16236577099942195
1205	17	-0.08051236685653151
1205	18	-0.23273060545887603
1205	19	-0.1653216273296252
1205	20	0.18978636953804795
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2111	19	-0.240607061859464
2111	20	-0.28687480983737146
2111	21	0.08206511544558737
2111	22	-0.08026088262978703
2111	23	-1.4228220084314245
2111	24	-1.1197662225811058
2111	25	-1.172039605301272
2112	1	-0.2323726709291698
2112	2	-0.21435417740071472
2112	3	-0.14655452488801402
2112	4	-0.02721622684806846
2112	5	-0.2770887480173627
2112	6	-0.2763876422230638
2112	7	-0.3864525279629092
2112	8	-0.4649645884419513
2112	9	-0.2661433992138953
2112	10	-0.2718476762547297
2112	11	-0.06286903662556398
2112	12	0.09319881014429399
2112	13	0.0959938532527218
2112	14	-0.7303879214028726
2112	15	-1.0869853550647264
2112	16	-0.9985167590274457
2112	17	-0.6536970036248704
2112	18	-1.4882598350783027
2112	19	-1.2819639959121716
2112	20	-0.6439720899176464
2112	21	-0.461989637075483
2112	22	-0.9203627111923893
2112	23	-0.42529067555736333
2112	24	-0.1528033532373385
2112	25	-0.1106144756643701
2113	1	0.03893930155291159
2113	2	0.08456021681734072
2113	3	-0.002527842049932616
2113	4	0.1527288963761464
2113	5	0.12452574263270577
2113	6	0.29728840585376304
2113	7	0.13965370631718343
2113	8	0.30040737071858814
2113	9	-0.017706691422787912
2113	10	0.2933234657190482
2113	11	0.5188794647016195
2113	12	0.263639912054046
2113	13	0.49404930663231994
2113	14	-0.17767340987577285
2113	15	0.33211116642370797
2113	16	0.3603246649851215
2113	17	0.14898519820634704
2113	18	0.3547001307231241
2113	19	0.02659359984880183
2113	20	0.593031956250428
2113	21	0.4534931691955819
2113	22	-1.7408798651555486
2113	23	-2.5065967825869535
2113	24	-1.0572809800156122
2113	25	-1.6122349311813764
2114	1	-0.5583565254429921
2114	2	-0.5640209955270805
2114	3	-0.2655187526999043
2114	4	-0.17451019598210848
2114	5	-0.20888127356955977
2114	6	-0.021135099379712585
2114	7	-0.29597230052583967
2114	8	-0.19462272562908112
2114	9	-0.4617726081876228
2114	10	-0.41599480491473173
2114	11	-0.0948375677788249
2114	12	-0.6296301594405946
2114	13	-0.11930294293261312
2114	14	-0.7555412080489035
2114	15	-0.3269949818904223
2114	16	-3.2297338408528162
2114	17	-2.6511499612438527
2114	18	-1.3876018251880424
2114	19	-1.9904457685917833
2114	20	-1.2963530717654663
2114	21	-1.6332819389741502
2114	22	-1.9906929051256341
2114	23	-2.959936197978408
2114	24	-7.421227143961286
2114	25	-3.649390074269057
2201	1	0.5445776579892012
2201	2	0.5863919317778858
2201	3	0.5939565536945217
2201	4	0.4358523360303934
2201	5	0.36322472587419696
2201	6	0.5002281748754385
2201	7	0.5618603772768083
2201	8	0.6114525654993628
2201	9	0.824796002318358
2201	10	1.0508792173823736
2201	11	0.8001630639170472
2201	12	0.8988934313292773
2201	13	0.8781550295517704
2201	14	1.0561591373659027
2201	15	1.0959235363616386
2201	16	1.1896236016211859
2201	17	1.1395579022291287
2201	18	1.1147884198485372
2201	19	1.142607147342659
2201	20	1.1041124968617098
2201	21	1.3376459038107882
2201	22	1.2772218141776719
2201	23	1.254390532670861
2201	24	1.4113327640567732
2201	25	1.6319841537678883
2202	1	0.3721535618128726
2202	2	0.4756123619502439
2202	3	0.3617624867262812
2202	4	0.3263742183357081
2202	5	0.1886883827117316
2202	6	0.3824939199675015
2202	7	0.4699951855654092
2202	8	0.4884854368573883
2202	9	0.6177937432225562
2202	10	0.8691428158376766
2202	11	0.8322541759279503
2202	12	0.7256232572803754
2202	13	0.7772836461617487
2202	14	0.8550606653171613
2202	15	0.868956983681457
2202	16	0.8178384764598121
2202	17	0.8282384039483048
2202	18	0.8184897515011471
2202	19	0.8798566457208423
2202	20	0.8882314222534902
2202	21	1.1437380217897015
2202	22	1.004050584434033
2202	23	1.058072061170563
2202	24	1.2672183334536982
2202	25	1.2366639148116292
2203	1	0.15789666238149636
2203	2	0.2535654485345873
2203	3	-0.43913421737667946
2203	4	-0.08361078820230006
2203	5	0.03304417242154045
2203	6	0.2558305481975438
2203	7	0.26160483113207533
2203	8	0.329743209232781
2203	9	0.355574939241734
2203	10	0.6449215538745321
2203	11	0.507689410802648
2203	12	0.5728621258719855
2203	13	0.5972964167635482
2203	14	0.7359201393889663
2203	15	0.6852108100595231
2203	16	0.6171166798505467
2203	17	0.5346028218258354
2203	18	0.6209094379512692
2203	19	0.8127779664733694
2203	20	0.7047744594736969
2203	21	0.8837352615833751
2203	22	0.9337564153123523
2203	23	0.8023494797880772
2203	24	0.9756706995781599
2203	25	1.0349392196316245
2204	1	0.0029660034859304574
2204	2	0.112979487710966
2204	3	0.019337427054527012
2204	4	0.03471627736684724
2204	5	0.05267817272228825
2204	6	0.17447036500530544
2204	7	0.04405671905752939
2204	8	-0.001261514042504075
2204	9	0.22878826842138267
2204	10	0.48368617785483536
2204	11	0.3705709581258603
2204	12	0.3390581444275895
2204	13	0.4643633369187228
2204	14	0.6335188686451048
2204	15	0.42566946874044476
2204	16	0.41260590838627564
2204	17	0.3713626699915409
2204	18	0.45652196218384233
2204	19	0.53361176962661
2204	20	0.3501121220231127
2204	21	0.7728502295840016
2204	22	0.5909264652143449
2204	23	0.6744758750453599
2204	24	0.6889931931921254
2204	25	0.7469635868284286
2205	1	-0.06863005350324514
2205	2	0.05827784132097591
2205	3	-0.13066850169758482
2205	4	-0.17139960178895564
2205	5	-0.18738760422801448
2205	6	-0.010797858008160688
2205	7	0.035148049916848834
2205	8	0.09192859506456585
2205	9	0.24349870018307485
2205	10	0.3354532937242851
2205	11	0.03617225250853551
2205	12	0.29849354519964066
2205	13	0.058375958420576524
2205	14	0.09400397193633125
2205	15	0.14975247195690855
2205	16	0.4441370813870904
2205	17	0.3559914463941354
2205	18	0.35485068946493215
2205	19	0.41621758368462736
2205	20	0.40054961756366936
2205	21	0.7014702865566917
2205	22	0.541301994347954
2205	23	0.5089476919215272
2205	24	0.5848199956680062
2205	25	0.5121165713862794
2206	1	-0.4345626113876584
2206	2	-0.4205375051664717
2206	3	-0.17034965355399123
2206	4	-0.1764181091977477
2206	5	-0.26744757881414216
2206	6	-0.1699962479248711
2206	7	-0.18189753812195164
2206	8	-0.09376634221828795
2206	9	-0.1434865396240994
2206	10	-0.19617729223853786
2206	11	-0.5362756665401207
2206	12	-0.1915290738042117
2206	13	-1.2754165105003352
2206	14	-0.709065122290184
2206	15	-0.44657169331157576
2206	16	-0.16101079703882704
2206	17	-0.09019986548118908
2206	18	-0.27008545451950994
2206	19	-0.35094178688112976
2206	20	-0.32104951342657984
2206	21	0.04030965994422786
2206	22	-0.047719643313357096
2206	23	-0.07336357151737616
2206	24	0.2111153125148988
2206	25	0.35697836290584206
2207	1	0.22826821990895851
2207	2	0.240127827891623
2207	3	0.2505698862826229
2207	4	0.10484242841985747
2207	5	0.13352542701716175
2207	6	0.3319643429534409
2207	7	0.2579420981085576
2207	8	0.28725062555575676
2207	9	0.4956215127029324
2207	10	0.5532216715156721
2207	11	0.4122071446215898
2207	12	0.3649727051642344
2207	13	0.4271994227766527
2207	14	0.5306860882409126
2207	15	0.3916857843814654
2207	16	0.5204023566451141
2207	17	0.5273872115932505
2207	18	0.5221871429797105
2207	19	0.7202996361871783
2207	20	0.4464028722235227
2207	21	0.7617952230992202
2207	22	0.7979970895585353
2207	23	0.5519559071891749
2207	24	0.7393154503616728
2207	25	0.7893326078146146
2208	1	0.24379856690656965
2208	2	0.248242477333374
2208	3	0.17547443919497496
2208	4	0.2308377603749463
2208	5	0.2324363733942505
2208	6	0.4594200075067292
2208	7	0.3994207201569111
2208	8	0.2267525439088871
2208	9	0.3178099410964421
2208	10	0.5103849872078925
2208	11	0.06343532982501898
2208	12	0.09427190237344973
2208	13	0.21836433212936868
2208	14	-0.2399489917401425
2208	15	0.22194039360524442
2208	16	0.41508335789055195
2208	17	0.31438774176771034
2208	18	0.4162272291879603
2208	19	0.5441653295079192
2208	20	0.5043951737139665
2208	21	0.8568129949234304
2208	22	0.8209103511353177
2208	23	0.7622014501160521
2208	24	0.8358258671917298
2208	25	0.839196456504709
2209	1	0.009532085527002465
2209	2	-0.04571852629140949
2209	3	0.044087030314109654
2209	4	-0.5719031154140382
2209	5	0.0036445723483211623
2209	6	0.06664758781915481
2209	7	0.09786043472459482
2209	8	0.16909607725595066
2209	9	0.1889580344737567
2209	10	0.14517750767606685
2209	11	0.22129117482334237
2209	12	0.15753740300217345
2209	13	0.15665096962971603
2209	14	0.19772459962187128
2209	15	-0.41385870312015527
2209	16	-0.3673595110669936
2209	17	0.18101700257671638
2209	18	-0.16856636733913888
2209	19	0.18772603921489406
2209	20	0.3133290760262639
2209	21	0.5810381556405133
2209	22	0.5238487605972537
2209	23	0.5769033356787219
2209	24	0.5954545961201916
2209	25	0.7565720410352839
2210	1	0.0047630080847014256
2210	2	-0.183807195721446
2210	3	-0.13169951071740016
2210	4	0.05909524619475803
2210	5	-0.01913880515362365
2210	6	-0.3642594919938986
2210	7	-0.12772157166286036
2210	8	-0.16963730288856027
2210	9	0.02968511775990379
2210	10	0.1948069443564009
2210	11	0.0880924613756946
2210	12	0.12408704172558771
2210	13	0.2080682912753602
2210	14	0.17935189204828106
2210	15	0.09165905115136752
2210	16	0.1876033797497101
2210	17	-0.890989611389621
2210	18	-1.497072184760448
2210	19	-0.22048464128371847
2210	20	-0.023534801600256117
2210	21	-0.2691274200237146
2210	22	-0.21416229288073652
2210	23	-0.19444543024273742
2210	24	0.3099924867481363
2210	25	0.38960895391364403
2211	1	-0.36759873979536195
2211	2	-0.3987212488178322
2211	3	-0.26508694123632637
2211	4	-0.1387046212671379
2211	5	-0.16509950354828362
2211	6	-0.08081999293384001
2211	7	-0.13992751964841688
2211	8	0.00822288293527862
2211	9	0.01798876526264337
2211	10	0.023284243824896578
2211	11	-0.36967034951406674
2211	12	-0.21232712632731676
2211	13	-0.23685347066997764
2211	14	-0.27585590876588384
2211	15	-0.3318499627921838
2211	16	-0.3099116660300325
2211	17	-0.25644747644419397
2211	18	-0.3841161792966261
2211	19	-0.2917733714875297
2211	20	-0.3690118711557133
2211	21	0.11760849104051374
2211	22	-0.1409038841494521
2211	23	-0.8102439723235904
2211	24	-0.29474913156916926
2211	25	-1.0261484743061544
2212	1	-0.09494544672579153
2212	2	-0.24361283226177477
2212	3	-0.10866744074655443
2212	4	-0.047396493358206726
2212	5	-0.06347311030425118
2212	6	-0.07660157168291448
2212	7	-0.1649227378436251
2212	8	-0.25890843991479784
2212	9	0.08385421396981485
2212	10	-0.34012016934622125
2212	11	-0.08281132871177022
2212	12	-0.2764960486534207
2212	13	-0.14853034818042232
2212	14	-0.18739252000225548
2212	15	0.03721784609791712
2212	16	-0.29183296621724253
2212	17	-0.40661643354705745
2212	18	-0.23067762693731453
2212	19	-0.18155364031190402
2212	20	-0.5373151708908281
2212	21	0.15163059746900132
2212	22	-0.3167861995862751
2212	23	-0.2536679764040599
2212	24	-0.4143029457105598
2212	25	-0.2579906573594357
2213	1	-0.2658850239215731
2213	2	-0.23540039577455119
2213	3	-0.21539259762300134
2213	4	0.02451546729805898
2213	5	-0.11439925247265847
2213	6	-0.2581093010698723
2213	7	-0.34243375889720795
2213	8	-0.3617765040394545
2213	9	-0.21474963021444182
2213	10	-0.3172086069850195
2213	11	-0.03023850398452055
2213	12	0.05677160987268337
2213	13	-0.02435086572621259
2213	14	-0.7161852613508017
2213	15	-0.3255074262756068
2213	16	-0.3636119353888674
2213	17	-0.3554958146865559
2213	18	-0.11860690875781899
2213	19	-0.1945216688532696
2213	20	-0.3507651449464504
2213	21	-0.025164681418218038
2213	22	-0.25817330246954384
2213	23	-0.37485795295645374
2213	24	-0.6298871528443684
2213	25	-0.3230414123790979
2214	1	-0.19025315440866208
2214	2	-0.11062342575955952
2214	3	-0.029784134637889537
2214	4	-0.021509279303380424
2214	5	-0.058040238067214034
2214	6	0.1282189178097397
2214	7	-0.023060501561062097
2214	8	-0.07591799956438905
2214	9	-0.15807625629326338
2214	10	0.132481475161633
2214	11	0.1714050488220238
2214	12	-0.13592887953653587
2214	13	-0.010348266837247877
2214	14	-0.003498496334024992
2214	15	0.22691933966393663
2214	16	-0.1424839459607128
2214	17	-0.07957406644824516
2214	18	-0.17413543559269584
2214	19	-0.2246106604857232
2214	20	-0.1984269712340634
2214	21	-0.13745954720082665
2214	22	-0.47518173611902625
2214	23	-0.6913079766797523
2214	24	-0.255750264252967
2214	25	-0.6534117437271121
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	20.0
10	46.0
11	115.0
12	418.0
13	1102.0
14	2376.0
15	4408.0
16	6782.0
17	9243.0
18	10860.0
19	11519.0
20	12065.0
21	12344.0
22	12629.0
23	12896.0
24	13821.0
25	14717.0
26	16378.0
27	17800.0
28	20726.0
29	25593.0
30	31788.0
31	42029.0
32	54965.0
33	73684.0
34	99411.0
35	137569.0
36	195782.0
37	307740.0
38	633762.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.00404902401732	22.80076117833281	12.970802218849439	21.22438757880043
2	14.266368525040418	24.249774766941286	40.2361609291607	21.2476957788576
3	16.624712306943003	31.82456804611403	31.257917352252022	20.292802294690944
4	10.5195596484279	19.84179704375127	38.970227682543815	30.668415625277014
5	11.635346108440277	40.090202309933254	33.45845845845846	14.815993123168012
6	26.856071360039856	41.79469512749357	17.29329636208779	14.055937150378783
7	21.9337997472656	38.34453355239974	23.58992833206517	16.131738368269488
8	23.401967853345266	37.550704347850484	21.5827556119171	17.46457218688715
9	24.7441094402664	16.633811997993938	23.43024058144349	35.19183798029618
10	16.00589588037468	31.435946509022646	31.240618718237407	21.317538892365263
11	29.52082050429961	29.180814857185837	22.40955496140484	18.888809677109712
12	20.253768150739493	31.391214492317843	31.904798235358793	16.45021912158387
13	26.043168454132676	26.906627293422297	28.1518323256281	18.898371926816925
14	20.212018772811064	25.07200245935942	30.99482883778438	23.721149930045137
15	22.72477124436	30.20584220951778	27.68382214757382	19.385564398548397
16	19.047771313426423	32.9945018419755	28.26572691415951	19.691999930438566
17	16.840869337898955	31.310424447912546	31.363885744290076	20.48482046989843
18	17.057922989625776	29.153799160884354	34.60945331823172	19.17882453125815
19	17.964508885120335	27.882902769962982	36.478948853548246	17.67363949136844
20	20.42204810632601	26.188984249349122	35.15272414959556	18.236243494729308
21	21.033537604706844	27.95406250210367	33.736808564587825	17.275591328601656
22	19.00545853365722	27.537208349929593	35.646132182908566	17.811200933504626
23	18.678376928928373	28.323074886289433	35.47713559325837	17.52141259152382
24	18.49806770683447	26.467732190730715	35.70167570123814	19.332524401196682
25	19.39288643449948	26.86279217711995	34.955564057656204	18.78875733072436
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2748.0
1	2748.0
2	3156.5
3	3565.0
4	3565.0
5	3565.0
6	8124.5
7	12684.0
8	12684.0
9	12684.0
10	14658.0
11	16632.0
12	16632.0
13	16632.0
14	19088.5
15	21545.0
16	21545.0
17	21545.0
18	34858.0
19	48171.0
20	48171.0
21	48171.0
22	70229.0
23	92287.0
24	92287.0
25	92287.0
26	122357.0
27	152427.0
28	152427.0
29	152427.0
30	176719.0
31	201011.0
32	201011.0
33	201011.0
34	213331.0
35	225651.0
36	225651.0
37	225651.0
38	220781.0
39	215911.0
40	215911.0
41	215911.0
42	206115.5
43	196320.0
44	196320.0
45	196320.0
46	195276.5
47	194233.0
48	194233.0
49	194233.0
50	174149.0
51	154065.0
52	154065.0
53	154065.0
54	129609.0
55	105153.0
56	105153.0
57	105153.0
58	84649.5
59	64146.0
60	64146.0
61	64146.0
62	53418.0
63	42690.0
64	42690.0
65	42690.0
66	30955.5
67	19221.0
68	19221.0
69	19221.0
70	14074.5
71	8928.0
72	8928.0
73	8928.0
74	6250.5
75	3573.0
76	3573.0
77	3573.0
78	2369.5
79	1166.0
80	1166.0
81	1166.0
82	756.0
83	346.0
84	346.0
85	346.0
86	214.5
87	83.0
88	83.0
89	83.0
90	58.0
91	33.0
92	33.0
93	33.0
94	20.5
95	8.0
96	8.0
97	8.0
98	4.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09396397841801685
2	0.0
3	0.13962766703429488
4	0.011443971102851006
5	0.02142939686906414
6	0.017502544039654483
7	0.027487969805867613
8	0.025412347596036795
9	0.0
10	0.39947312854608835
11	0.06490526748038537
12	0.0014585453366378735
13	0.0
14	0.0011219579512599027
15	0.0010658600536969077
16	5.609789756299513E-5
17	0.0
18	3.9268528294096594E-4
19	6.170768731929465E-4
20	2.804894878149757E-4
21	1.1219579512599026E-4
22	0.003814657034283669
23	1.1219579512599026E-4
24	5.609789756299513E-5
25	0.001795132722015844
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1782598.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.9766504469414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.61316337982907	54.77243427208208
2	10.392082439458996	13.297012512901324
3	2.2319907885200845	4.283858834338276
4	0.6620028878167664	1.694109093948761
5	0.28549220164811384	0.9132417395084543
6	0.17309443269605906	0.6644401208944437
7	0.10843003372275857	0.4855893255801696
8	0.07547781000343103	0.3863053973672131
9	0.05392355374217101	0.310486351375776
>10	0.3277176834035324	4.020467652086275
>50	0.03371961504380043	1.5297713352373274
>100	0.034783640288062405	4.5493865905946524
>500	0.004766987246481627	2.156292402673429
>1k	0.00273660378964686	3.243418449270098
>5k	2.648326248045348E-4	1.1821203845038644
>10k+	3.531101664060464E-4	6.51106553763781
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	43277	2.4277487128337403	No Hit
GGTATCAACGCAGAGTACTTTTTTT	29951	1.6801881299092671	No Hit
TATCAACGCAGAGTACTTTTTTTTT	27285	1.5306311350063222	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	14774	0.8287903385956901	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	8340	0.4678564656753794	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	7111	0.3989121495704584	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	5480	0.3074164786452133	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	4475	0.25103809159440327	No Hit
ATTCCATTCCATTCCATTCCATTCC	3270	0.18344012503099408	No Hit
GTGGTATCAACGCAGAGTACTTTTT	2981	0.1672278326352885	No Hit
GAATGGAATGGAATGGAATGGAATG	2961	0.1661058746840286	No Hit
CCCATGTACTCTGCGTTGATACCAC	2903	0.1628521966253749	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2688	0.1507911486493309	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2546	0.1428252471953856	No Hit
GTTAATGATAGTGTGTCGAAACACA	2400	0.1346349541511883	No Hit
GGTATCAACGCAGAGTACATGGGGG	1943	0.10899821496489955	No Hit
ATACAGGGTGACAGCCCCGTACACA	1823	0.10226646725734012	No Hit
ACTTAGATGTTTCAGTTCCCCCGGT	1814	0.10176158617927317	No Hit
CAACGCAGAGTACTTTTTTTTTTTT	1792	0.10052743243288728	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.6097897562995135E-5	0.0	0.0	0.0	0.0
2	5.6097897562995135E-5	0.0	0.0	0.0	0.0
3	5.6097897562995135E-5	0.0	0.0	0.0	0.0
4	5.6097897562995135E-5	0.0	0.0	0.0	0.0
5	5.6097897562995135E-5	0.0	0.0	0.0	0.0
6	5.6097897562995135E-5	0.0	0.0	0.0	0.0
7	5.6097897562995135E-5	0.0	0.0	0.0	0.0
8	5.6097897562995135E-5	0.0	0.0	0.0	0.0
9	5.6097897562995135E-5	0.0	0.0	0.0	0.0
10	5.6097897562995135E-5	0.0	0.0	0.0	0.0
11	5.6097897562995135E-5	0.0	0.0	0.0	0.0
12	5.6097897562995135E-5	0.0	0.0	0.0	0.0
13	5.6097897562995135E-5	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6020	0.0	14.260844	1
GCCCGCC	40	0.0053258943	14.231219	11
TAGGACC	70	7.0721126E-6	13.606638	4
ATCGCCG	170	0.0	13.38432	19
CCCGGGT	50	0.001523347	13.27241	16
AGGACCG	65	5.3223685E-5	13.187972	5
AAATCGC	190	0.0	12.973034	17
AATCGCC	190	0.0	12.973034	18
GAAATCG	200	0.0	11.376351	16
CGCGCGA	85	5.438979E-5	11.152974	13
CCCCGTA	410	0.0	11.098569	15
CGGGTTC	60	0.005967751	11.060342	18
CCCGTAC	405	0.0	11.0018215	16
CCGTACA	415	0.0	10.965159	17
ACAGGGT	395	0.0	10.578726	3
GACAGCT	100	2.3312765E-5	10.477111	7
GCCCCGT	410	0.0	10.404908	14
GTCATAT	165	9.1677066E-10	10.371524	1
GTATCAA	12845	0.0	10.332498	1
CGTACCA	75	0.0025933979	10.158764	10
>>END_MODULE