FastQCFastQC Report
Wed 25 May 2016
SRR1294681_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294681_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4368506
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC109060.2496505670359615No Hit
CCCATGTACTCTGCGTTGATACCAC102070.23364967336659262No Hit
GTATCAACGCAGAGTACTTTTTTTT92990.21286453538120353No Hit
GGTATCAACGCAGAGTACTTTTTTT53670.1228566471008624No Hit
TATCAACGCAGAGTACTTTTTTTTT50740.11614954860998244No Hit
GAGTACATGGGAAGCAGTGGTATCA48760.1116171066263844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGG852.7054557E-713.4106811
CGTCGGT951.0435087E-611.99779319
GCGTCGG907.41409E-611.61882518
GGACCGT752.0818782E-411.3962076
TCTGTCG2550.010.8000788
CTGTCGC2500.010.6364599
TATTCCG1451.4531906E-810.479395
AGCGTCG1002.387793E-510.45526517
TGTCGCC2550.010.42778110
CCCCCGG2200.010.37052218
GCGACTA1659.222276E-1010.36885616
CGAACCG1201.5162223E-610.29661612
ATCGCCG750.00265325610.13147119
ACCGACG856.5682497E-410.06377515
CGACTAA1803.9108272E-1010.03282917
CCCCGGT2201.8189894E-129.92999219
GGCGAAC1252.5843237E-69.87659910
TCGCCCA2450.09.6985412
CGACGCT800.00449948159.50478617
TCGGAAC900.00110935829.50467713
Produced by FastQC (version 0.11.5)