##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294681_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4368506 Sequences flagged as poor quality 0 Sequence length 25 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.06228674059278 31.0 30.0 33.0 25.0 34.0 2 30.15534006362816 31.0 30.0 34.0 25.0 34.0 3 30.071968311363197 31.0 30.0 34.0 25.0 34.0 4 33.73255273084208 35.0 35.0 37.0 28.0 37.0 5 34.04554211439792 35.0 35.0 37.0 28.0 37.0 6 34.18446832853154 37.0 35.0 37.0 30.0 37.0 7 34.05852641612487 37.0 35.0 37.0 29.0 37.0 8 33.89920512870991 37.0 35.0 37.0 28.0 37.0 9 35.09338776231508 39.0 35.0 39.0 27.0 39.0 10 35.18085061574827 38.0 35.0 39.0 27.0 39.0 11 35.43518539290091 39.0 35.0 39.0 28.0 39.0 12 35.210899561543464 39.0 35.0 39.0 27.0 39.0 13 35.07317970949336 38.0 35.0 39.0 27.0 39.0 14 35.975544957475165 39.0 35.0 41.0 27.0 41.0 15 35.960994674151756 39.0 35.0 41.0 27.0 41.0 16 36.075625854697236 39.0 35.0 41.0 27.0 41.0 17 36.05294624752719 39.0 35.0 41.0 27.0 41.0 18 35.953568565546206 39.0 35.0 41.0 27.0 41.0 19 36.2377931952022 39.0 35.0 41.0 27.0 41.0 20 36.36248067417099 39.0 36.0 41.0 27.0 41.0 21 36.39409171007205 39.0 36.0 41.0 27.0 41.0 22 36.38916989011804 39.0 36.0 41.0 27.0 41.0 23 36.360280837430466 39.0 36.0 41.0 27.0 41.0 24 36.370188114655214 39.0 36.0 41.0 27.0 41.0 25 35.18770215721347 38.0 33.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.988202561507503 1101 2 1.0264384074845943 1101 3 1.285442206109174 1101 4 1.1502895945172753 1101 5 0.8500490914639798 1101 6 0.8496526479301352 1101 7 0.8710385986837252 1101 8 1.0167402846233244 1101 9 1.2871118765955316 1101 10 1.2060750085607737 1101 11 0.9920064361628889 1101 12 1.2552164332688776 1101 13 1.2876848045717608 1101 14 1.390588037602349 1101 15 1.4408523836454492 1101 16 1.3992997362777473 1101 17 1.4273784931141336 1101 18 1.388178175832465 1101 19 1.2838807421559935 1101 20 1.179746221415975 1101 21 1.1419133657570057 1101 22 1.0851269361036202 1101 23 1.1466529690666292 1101 24 1.1597629747839946 1101 25 1.1433605029443967 1102 1 0.8738363127066684 1102 2 0.8166070628764288 1102 3 1.1120095023891032 1102 4 1.0409316275800578 1102 5 0.807229431289862 1102 6 0.6798530654366601 1102 7 0.6681257241285081 1102 8 0.7732516678442565 1102 9 1.123294578219685 1102 10 0.96550528461092 1102 11 0.7602661489385198 1102 12 0.9928604676323189 1102 13 1.0582604150522954 1102 14 1.2022476500135255 1102 15 1.3891466670636348 1102 16 1.171668552875488 1102 17 1.2210826599155382 1102 18 1.2245469948255376 1102 19 1.0124954492606761 1102 20 0.20941948602720117 1102 21 0.578357787445789 1102 22 0.7517520736855587 1102 23 0.8082840770158981 1102 24 0.864513219407371 1102 25 0.8141164520531561 1103 1 0.6458984045188068 1103 2 0.68372650209227 1103 3 1.0036243167474197 1103 4 0.7193276957623027 1103 5 0.6474396631297523 1103 6 0.48513508263836513 1103 7 0.4649615210205411 1103 8 0.5648124102272902 1103 9 0.6658340233552948 1103 10 0.6544293589653662 1103 11 0.5982399638808147 1103 12 0.824547474744044 1103 13 0.7648104719241644 1103 14 0.6822583518203587 1103 15 0.889672394805757 1103 16 0.8017544665777976 1103 17 0.8623104226305287 1103 18 0.8985928318612011 1103 19 0.7743639219214344 1103 20 -0.1561339398990782 1103 21 0.2777781654992637 1103 22 -0.18847101691448387 1103 23 0.3861509212954246 1103 24 0.5210907863225529 1103 25 0.6184273069503377 1104 1 0.3392886435670377 1104 2 0.30673071105123384 1104 3 0.5970022311382266 1104 4 0.36112252559799884 1104 5 0.3250914620727201 1104 6 0.11885457874268468 1104 7 0.18605862192498535 1104 8 0.3614991118334814 1104 9 0.6136619793934202 1104 10 0.5153775025269809 1104 11 0.2643553232639917 1104 12 0.38665059792428025 1104 13 0.26826226054744495 1104 14 0.5900650198138422 1104 15 0.6767517311023212 1104 16 0.4643822427584823 1104 17 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0.3486896225040752 2207 9 -1.3902210340493255 2207 10 -0.16596202923295778 2207 11 0.12142644776071876 2207 12 0.42982561952432263 2207 13 0.47311207462962557 2207 14 0.8059601782550061 2207 15 0.8117688117553215 2207 16 0.7727456552511853 2207 17 0.7642367180200438 2207 18 0.7518105203993883 2207 19 -0.4379411802343611 2207 20 0.2461959935058431 2207 21 -0.32653120592529206 2207 22 0.19996135776897006 2207 23 -0.5082517580410837 2207 24 -1.4624954949043953 2207 25 -0.18846287399826878 2208 1 0.5808795526579473 2208 2 0.26551858983911103 2208 3 0.7660779887139846 2208 4 0.6074634346889027 2208 5 0.48684146207271795 2208 6 0.47027882116692865 2208 7 0.4762177128340781 2208 8 0.667241536075899 2208 9 0.003957433938879262 2208 10 0.19926386616334923 2208 11 0.42331744447611186 2208 12 0.37264302216670586 2208 13 0.6950046847898648 2208 14 0.9492392622380876 2208 15 0.8374638523144426 2208 16 0.7461398185160562 2208 17 0.569842625589331 2208 18 0.8655590643637936 2208 19 -1.1800545175204178 2208 20 0.024245640384414457 2208 21 -0.10718894049131933 2208 22 0.2922690430134267 2208 23 0.5151638522345863 2208 24 0.6059019083676205 2208 25 0.6928841688065575 2209 1 0.2294729467353953 2209 2 -1.2686251252157668 2209 3 -0.1198005575688903 2209 4 0.15724056245863238 2209 5 0.15084047843221526 2209 6 0.18183090234315102 2209 7 0.18261732973198974 2209 8 0.35993619335484084 2209 9 -2.030399316941221 2209 10 -0.3088850777173704 2209 11 -0.005168525704050353 2209 12 0.38582199296811126 2209 13 0.43144856551051447 2209 14 0.5593979389827552 2209 15 0.7445362275474068 2209 16 0.6759285946601921 2209 17 0.4709455972190639 2209 18 0.7076029657926597 2209 19 0.5563932459916998 2209 20 0.3751868290363092 2209 21 0.4547964602127621 2209 22 0.316908461112412 2209 23 0.43852401997118307 2209 24 0.4459011318093289 2209 25 0.5069504868848327 2210 1 0.2119105529955192 2210 2 0.19803247631271503 2210 3 0.1714801849761436 2210 4 0.27462469935537115 2210 5 0.0982835199304688 2210 6 0.17332162117102 2210 7 0.027098711125759678 2210 8 0.19045105048407862 2210 9 0.4098466413613622 2210 10 -1.5038324977798965 2210 11 -0.44643447865982466 2210 12 -2.356128715407074 2210 13 -0.7554061553545708 2210 14 -0.4445708357600111 2210 15 -0.8338161327505702 2210 16 -0.18877890853161716 2210 17 0.05799095512071517 2210 18 0.3157410462167576 2210 19 0.21283403521749733 2210 20 0.24382515898666668 2210 21 0.18067440944781765 2210 22 0.18649619554470576 2210 23 0.15999395391528992 2210 24 0.13212106658647116 2210 25 0.10549417371669279 2211 1 -0.34644301860441473 2211 2 -0.130498121779695 2211 3 -1.466428093046023 2211 4 -0.6627293967670127 2211 5 -0.5059925719851321 2211 6 -0.43113135388199453 2211 7 -0.46160270336984155 2211 8 -0.07966879762762602 2211 9 0.14878747518907431 2211 10 0.043869543354105645 2211 11 -0.27753710891116157 2211 12 -0.27124394964566534 2211 13 -0.26412654901414356 2211 14 -0.8021010386768168 2211 15 -0.36640718793094607 2211 16 -0.06917828397481429 2211 17 -0.12919612579889872 2211 18 0.0865585883999671 2211 19 -0.0687844212699531 2211 20 -0.05097806261415627 2211 21 -0.19437612648740554 2211 22 -0.2470358514812574 2211 23 -0.5503046416043205 2211 24 -0.3730707369335633 2211 25 -0.18502178867400687 2212 1 -0.39490581904469124 2212 2 -0.40270464975103337 2212 3 -2.4496670516173857 2212 4 -1.4516500926710236 2212 5 -0.6713166478030175 2212 6 -0.4587586761079834 2212 7 -0.37434120366908274 2212 8 -0.35173219403092304 2212 9 -0.97769751482938 2212 10 -0.2541651179221134 2212 11 -0.4031162267687094 2212 12 -0.11400429634212372 2212 13 -0.5123721410222117 2212 14 -0.09555172315541682 2212 15 -0.03553429462255764 2212 16 -1.236288784056427 2212 17 -1.4931388982824814 2212 18 -0.38368008976744505 2212 19 -0.2737881669644935 2212 20 -0.2431328204832539 2212 21 -0.20475107260380554 2212 22 -0.5462580444075442 2212 23 -0.2646500239372003 2212 24 -0.3273782856589804 2212 25 -0.10740932367218647 2213 1 -3.0692654302852347 2213 2 -1.3983019232556053 2213 3 -2.294850591880813 2213 4 -1.0719395098844586 2213 5 -0.8625086277562843 2213 6 -0.6558489605913707 2213 7 -0.3959949546595851 2213 8 -0.23461196244299032 2213 9 -0.3770390932076637 2213 10 -0.16923260751354974 2213 11 -0.6081431991914741 2213 12 -0.2612665312548188 2213 13 -0.6434307666543475 2213 14 -0.3027266161077762 2213 15 -0.47818406611227005 2213 16 -1.6367087233667164 2213 17 -1.109527194538785 2213 18 -0.4373024171731359 2213 19 -0.27014746113132304 2213 20 -0.25776925625875435 2213 21 -0.02917274453575658 2213 22 -0.1451683573993563 2213 23 -0.015282097974477438 2213 24 8.354207916028145E-5 2213 25 -0.049892095483201615 2214 1 -0.9091976222718934 2214 2 -0.8420696404525785 2214 3 -6.405597536415705 2214 4 -2.1952101557524593 2214 5 -1.3281841566023331 2214 6 -0.8402803250189734 2214 7 -0.730594835001618 2214 8 -0.5076119803795791 2214 9 -0.3564597996694232 2214 10 -0.31179691706651624 2214 11 -0.9110516557875172 2214 12 -0.6325229145626139 2214 13 -5.333606379076105 2214 14 -4.03834312146801 2214 15 -2.4212845531622165 2214 16 -2.956296613153299 2214 17 -3.423622911446131 2214 18 -1.2798801736666476 2214 19 -0.8445141867099721 2214 20 -0.6822476838778471 2214 21 -0.6155057616495796 2214 22 -0.5196646885964 2214 23 -0.5471527775808696 2214 24 -0.41783576065185457 2214 25 -0.20234172200048306 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1637.0 3 229.0 4 216.0 5 470.0 6 67.0 7 128.0 8 362.0 9 885.0 10 1908.0 11 3555.0 12 6180.0 13 10049.0 14 14897.0 15 20066.0 16 24972.0 17 28085.0 18 29704.0 19 30581.0 20 31103.0 21 31950.0 22 32111.0 23 34030.0 24 36645.0 25 39170.0 26 43811.0 27 49585.0 28 57838.0 29 70483.0 30 86576.0 31 108932.0 32 141903.0 33 188678.0 34 253730.0 35 349450.0 36 491511.0 37 744731.0 38 1402278.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.39363429737058 22.075951130637346 13.453327568037254 23.07708700395482 2 14.143820627202885 22.426111626566854 41.5231095059328 21.906958240297453 3 17.509277298344447 28.371561009373725 30.449579641386208 23.669582050895617 4 11.407863772480804 18.528362601560843 40.17866692623491 29.885106699723448 5 11.919647376277782 39.538617345272506 35.90389143894425 12.637843839505456 6 28.569079007648973 40.6463146012234 18.52837854182014 12.256227849307487 7 24.32562251902889 34.215400402681794 24.81649117100691 16.6424859072824 8 23.063627638935653 38.3088135927089 22.285867421613563 16.34169134674189 9 24.15144171301344 17.594190084386252 22.972233166271153 35.28213503632915 10 13.863172824778314 30.998455203654736 34.70420265235852 20.434169319208433 11 30.734773408008508 27.205537604694165 24.27445600949717 17.785232977800153 12 19.846280211073815 28.56890248120304 33.06968193710022 18.515135370622918 13 25.549084384081116 25.084616795915764 27.738733660432004 21.62756515957112 14 19.445432566492087 24.168269104127702 29.50476951814639 26.881528811233824 15 21.923067584326365 31.596816747671223 25.20823035011469 21.27188531788772 16 20.388571449513243 32.43439174793018 26.972393387019856 20.20464341553672 17 18.931991355328304 31.198560595735614 29.79857731701367 20.07087073192241 18 19.38095453139825 30.80501264347554 30.470558272885885 19.343474552240327 19 19.88229009515109 29.95621556219044 30.671784395660683 19.489709946997785 20 21.227483229670256 29.483254408295856 29.727290593635086 19.561971768398802 21 21.38962827026902 29.927801612822318 29.076519845795367 19.606050271113293 22 20.16543434245793 29.641842963588733 30.168116677688445 20.024606016264894 23 20.09259982495229 30.01644422261249 29.761271301727778 20.129684650707443 24 20.139733325513863 29.38376769575174 29.914170621455465 20.562328357278933 25 20.60413739833059 29.341653424569987 29.20835817777449 20.845850999324934 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4258.0 1 4258.0 2 4356.0 3 4454.0 4 4454.0 5 4454.0 6 7358.0 7 10262.0 8 10262.0 9 10262.0 10 16876.0 11 23490.0 12 23490.0 13 23490.0 14 39503.0 15 55516.0 16 55516.0 17 55516.0 18 86383.0 19 117250.0 20 117250.0 21 117250.0 22 163665.0 23 210080.0 24 210080.0 25 210080.0 26 266871.5 27 323663.0 28 323663.0 29 323663.0 30 378265.0 31 432867.0 32 432867.0 33 432867.0 34 471102.0 35 509337.0 36 509337.0 37 509337.0 38 524055.0 39 538773.0 40 538773.0 41 538773.0 42 523078.0 43 507383.0 44 507383.0 45 507383.0 46 498345.5 47 489308.0 48 489308.0 49 489308.0 50 445875.5 51 402443.0 52 402443.0 53 402443.0 54 350744.5 55 299046.0 56 299046.0 57 299046.0 58 251660.0 59 204274.0 60 204274.0 61 204274.0 62 166132.0 63 127990.0 64 127990.0 65 127990.0 66 96079.0 67 64168.0 68 64168.0 69 64168.0 70 45764.5 71 27361.0 72 27361.0 73 27361.0 74 19056.5 75 10752.0 76 10752.0 77 10752.0 78 7285.5 79 3819.0 80 3819.0 81 3819.0 82 2552.5 83 1286.0 84 1286.0 85 1286.0 86 875.5 87 465.0 88 465.0 89 465.0 90 324.0 91 183.0 92 183.0 93 183.0 94 119.0 95 55.0 96 55.0 97 55.0 98 39.0 99 23.0 100 23.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10216307359999048 2 0.05400015474397883 3 0.05658685143158783 4 0.052214647295894756 5 0.04926169267021723 6 0.06762037181590227 7 0.06354575225488988 8 0.060340995296790254 9 0.06560595315652537 10 0.06597221109459389 11 0.05894463690790399 12 0.060432559781307384 13 0.06059279762921237 14 0.06320238543795063 15 0.06656738024395525 16 0.061073511172927315 17 0.06434694149441479 18 0.1413984552155817 19 0.06931431477946923 20 0.06317949431682135 21 0.05995184623759244 22 0.06654448912282597 23 0.06498789288603472 24 0.07631899784502987 25 0.06887938347801285 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4368506.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.0768558931397 #Duplication Level Percentage of deduplicated Percentage of total 1 90.02503630343531 72.98946895148293 2 7.76469049068124 12.5907338393559 3 1.2069791569232304 2.9357422551566397 4 0.35672939787897495 1.1568999193872056 5 0.16842055120753185 0.6827504379848107 6 0.1019976322684796 0.49617883997237855 7 0.06448141762699337 0.3659565423310369 8 0.04599724739703863 0.2983449758952636 9 0.03766664746361187 0.27485040135464595 >10 0.20058578955366715 2.9691732376774453 >50 0.015537633905163988 0.864454411227622 >100 0.009831673318750376 1.5085340289141957 >500 0.0011651168779340966 0.6601881525863907 >1k 7.388541294264406E-4 1.2654713615503812 >5k 8.525239954913189E-5 0.4548093705415381 >10k+ 5.6834933032754585E-5 0.48644327458173725 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 10906 0.2496505670359615 No Hit CCCATGTACTCTGCGTTGATACCAC 10207 0.23364967336659262 No Hit GTATCAACGCAGAGTACTTTTTTTT 9299 0.21286453538120353 No Hit GGTATCAACGCAGAGTACTTTTTTT 5367 0.1228566471008624 No Hit TATCAACGCAGAGTACTTTTTTTTT 5074 0.11614954860998244 No Hit GAGTACATGGGAAGCAGTGGTATCA 4876 0.1116171066263844 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.2891121129283102E-5 0.0 2 0.0 0.0 0.0 4.5782242258566204E-5 0.0 3 0.0 0.0 0.0 4.5782242258566204E-5 0.0 4 0.0 0.0 0.0 6.86733633878493E-5 0.0 5 0.0 2.2891121129283102E-5 0.0 6.86733633878493E-5 0.0 6 0.0 2.2891121129283102E-5 0.0 6.86733633878493E-5 0.0 7 0.0 2.2891121129283102E-5 0.0 9.156448451713241E-5 2.2891121129283102E-5 8 0.0 2.2891121129283102E-5 0.0 9.156448451713241E-5 2.2891121129283102E-5 9 0.0 2.2891121129283102E-5 0.0 1.373467267756986E-4 2.2891121129283102E-5 10 0.0 2.2891121129283102E-5 0.0 1.373467267756986E-4 2.2891121129283102E-5 11 0.0 2.2891121129283102E-5 0.0 1.602378479049817E-4 2.2891121129283102E-5 12 0.0 2.2891121129283102E-5 0.0 1.602378479049817E-4 2.2891121129283102E-5 13 0.0 2.2891121129283102E-5 0.0 1.602378479049817E-4 2.2891121129283102E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTCGG 85 2.7054557E-7 13.410681 1 CGTCGGT 95 1.0435087E-6 11.997793 19 GCGTCGG 90 7.41409E-6 11.618825 18 GGACCGT 75 2.0818782E-4 11.396207 6 TCTGTCG 255 0.0 10.800078 8 CTGTCGC 250 0.0 10.636459 9 TATTCCG 145 1.4531906E-8 10.47939 5 AGCGTCG 100 2.387793E-5 10.455265 17 TGTCGCC 255 0.0 10.427781 10 CCCCCGG 220 0.0 10.370522 18 GCGACTA 165 9.222276E-10 10.368856 16 CGAACCG 120 1.5162223E-6 10.296616 12 ATCGCCG 75 0.002653256 10.131471 19 ACCGACG 85 6.5682497E-4 10.063775 15 CGACTAA 180 3.9108272E-10 10.032829 17 CCCCGGT 220 1.8189894E-12 9.929992 19 GGCGAAC 125 2.5843237E-6 9.876599 10 TCGCCCA 245 0.0 9.69854 12 CGACGCT 80 0.0044994815 9.504786 17 TCGGAAC 90 0.0011093582 9.504677 13 >>END_MODULE