FastQCFastQC Report
Wed 25 May 2016
SRR1294681_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294681_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4368506
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT239680.5486543912266574No Hit
GGTATCAACGCAGAGTACTTTTTTT158700.3632820923217228No Hit
TATCAACGCAGAGTACTTTTTTTTT142910.32713701205858475No Hit
CCCATGTACTCTGCGTTGATACCAC122510.28043912495484724No Hit
GTACATGGGAAGCAGTGGTATCAAC104220.2385712644093885No Hit
ACGCAGAGTACTTTTTTTTTTTTTT87240.1997021407318658No Hit
GAGTACATGGGAAGCAGTGGTATCA49580.11349417855898562No Hit
GTACTTTTTTTTTTTTTTTTTTTTT46590.10664973334132996No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGCG600.00578745611.1066094
AGTACCG700.001466445610.8799455
CCGTGCA909.324949E-510.5774819
TATACCG750.00260350210.1546145
GTCCTAC2350.010.1127361
CGCGATC1252.630566E-69.86290117
GGCACCG800.0044406889.5199515
CGACTAA2304.3655746E-119.0712317
TACGGAG950.00179032649.0189025
CCTACAC4600.08.8787843
CTAGACT4400.08.8708644
TATACTG11550.08.8193495
TCTAGAC4850.08.8127953
ACCGTAT1304.2661006E-58.7876478
CTTAGAC4000.08.7858673
TAGACAG7100.08.7154485
CTAGGAC4700.08.6898753
GCGACTA2304.1836756E-108.65890116
CGCCTCC2850.08.65731611
GCGAACC1107.27309E-48.62141618
Produced by FastQC (version 0.11.5)