##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294681_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4368506 Sequences flagged as poor quality 0 Sequence length 25 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.21806196443361 31.0 31.0 34.0 28.0 34.0 2 31.427685117062904 31.0 31.0 34.0 28.0 34.0 3 31.504198689437533 31.0 31.0 34.0 28.0 34.0 4 35.07734085749224 37.0 35.0 37.0 32.0 37.0 5 35.18491722341688 37.0 35.0 37.0 32.0 37.0 6 34.7082002405399 37.0 35.0 37.0 32.0 37.0 7 34.54206151943021 37.0 35.0 37.0 30.0 37.0 8 34.57953336907401 37.0 35.0 37.0 31.0 37.0 9 36.04588090299063 39.0 35.0 39.0 30.0 39.0 10 35.80655995436426 39.0 35.0 39.0 30.0 39.0 11 35.95831229257783 39.0 35.0 39.0 30.0 39.0 12 35.911515744742026 39.0 35.0 39.0 30.0 39.0 13 35.76387144712632 39.0 35.0 39.0 30.0 39.0 14 36.81431180362348 39.0 36.0 41.0 30.0 41.0 15 36.87227486925736 39.0 36.0 41.0 30.0 41.0 16 36.916905001389495 40.0 36.0 41.0 30.0 41.0 17 36.951850129083034 40.0 36.0 41.0 30.0 41.0 18 36.96224269807573 40.0 36.0 41.0 30.0 41.0 19 36.98279159969106 40.0 36.0 41.0 30.0 41.0 20 37.040529416693026 40.0 36.0 41.0 30.0 41.0 21 36.87817276661632 40.0 36.0 41.0 29.0 41.0 22 36.86209083837816 40.0 36.0 41.0 30.0 41.0 23 36.87201871761192 39.0 36.0 41.0 30.0 41.0 24 36.79552986764811 39.0 36.0 41.0 29.0 41.0 25 35.66276250965433 39.0 34.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5571674304818366 1101 2 0.5571197793216669 1101 3 0.5528585116326319 1101 4 0.434054623175399 1101 5 0.4484394007206376 1101 6 0.5734501828380232 1101 7 0.5802372722815434 1101 8 0.5969771016554191 1101 9 0.8152259159689734 1101 10 0.9192227506720201 1101 11 0.8131853446197468 1101 12 0.8795856508479929 1101 13 0.7983840482363007 1101 14 1.0791173968794396 1101 15 1.0351032702280136 1101 16 1.0877407218025397 1101 17 1.1460485754140208 1101 18 1.13297064503832 1101 19 1.2148357257545044 1101 20 1.1887998602222183 1101 21 1.3754466026905945 1101 22 1.2856407800793548 1101 23 1.3306886615249525 1101 24 1.3934228109258555 1101 25 1.3283673097415303 1102 1 0.43585747080726733 1102 2 0.39325325685404167 1102 3 0.28488468184259474 1102 4 0.2618311733450511 1102 5 0.2856429632993098 1102 6 0.35514540786158477 1102 7 0.4034291552168341 1102 8 0.41328797155650676 1102 9 0.49186710826989355 1102 10 0.6264071026805667 1102 11 0.6232938891316238 1102 12 0.6675205085712079 1102 13 0.6772822130519742 1102 14 0.875596856300703 1102 15 0.7437528473918675 1102 16 0.8125841568536885 1102 17 0.7285651900420831 1102 18 0.7537908489530096 1102 19 0.8314800559415616 1102 20 0.8147852702493239 1102 21 1.1066299450663095 1102 22 1.0072361736145936 1102 23 0.9567007196309376 1102 24 0.9308391988356561 1102 25 0.9035621401339924 1103 1 0.32315763782300877 1103 2 0.30281261914250024 1103 3 0.2673364740841393 1103 4 0.2372719488290258 1103 5 0.19303358013758753 1103 6 0.2306614396837574 1103 7 0.30781861447041337 1103 8 0.3465455843721301 1103 9 0.23619483954906428 1103 10 0.4828992803175183 1103 11 0.4914960715061696 1103 12 0.46580010127442506 1103 13 0.6075000623739726 1103 14 0.7504742166164959 1103 15 0.7201327567939018 1103 16 0.67984522020204 1103 17 0.6253389448978197 1103 18 0.6463630483728267 1103 19 0.762893829214363 1103 20 0.6539678975310466 1103 21 0.8389645714852563 1103 22 0.7842661614876505 1103 23 0.8028399657816792 1103 24 0.911959457575243 1103 25 0.7875869083578024 1104 1 0.13387038927329087 1104 2 0.12937328785836044 1104 3 0.09240736297794072 1104 4 0.04957826669019738 1104 5 0.11047757002429393 1104 6 0.07736472650477566 1104 7 0.1316675931897393 1104 8 0.125750231180767 1104 9 -0.04091567695137854 1104 10 0.3516914196628136 1104 11 0.32630736124618664 1104 12 0.4011184120887208 1104 13 -0.1161516628569359 1104 14 0.2600985357012888 1104 15 0.4369631969286516 1104 16 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0.08655530331186867 1107 14 0.1823788289311281 1107 15 0.2300627304266314 1107 16 0.08888659535679722 1107 17 0.2359569639277197 1107 18 0.18129179688813224 1107 19 0.303466657217065 1107 20 0.27372673720739016 1107 21 0.6420958829319048 1107 22 0.6079553723841613 1107 23 0.5202897115794727 1107 24 0.5041058384095933 1107 25 0.5029420824141582 1108 1 0.08561659386217002 1108 2 0.08874014628943883 1108 3 0.020269108282196413 1108 4 0.009999623786718814 1108 5 0.10310461483194899 1108 6 0.12502164898729262 1108 7 0.10750522084067882 1108 8 0.09569475774243585 1108 9 0.04182834769087407 1108 10 -0.8164548884292202 1108 11 -0.11908847097944886 1108 12 0.0343585500456669 1108 13 0.25581130286296627 1108 14 0.31562806423023204 1108 15 -0.004115192533880929 1108 16 0.1073880023932432 1108 17 0.2462555866936711 1108 18 0.20347310623919412 1108 19 0.27154605649522523 1108 20 0.21892114727207002 1108 21 0.4427759481389444 1108 22 0.452015776873246 1108 23 0.363243228965608 1108 24 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0.2707589173068712 2207 4 0.1905851402275971 2207 5 0.1473367587020249 2207 6 0.27905787640386137 2207 7 0.24962591821536506 2207 8 0.24200948771937192 2207 9 0.3665425553776842 2207 10 0.580259156362601 2207 11 0.43944042803318695 2207 12 0.4217533520458332 2207 13 0.5096418092064212 2207 14 0.47805818996060623 2207 15 0.47052584406257836 2207 16 0.5600933883663686 2207 17 0.5653811220519174 2207 18 0.574379044961546 2207 19 0.5947748331894473 2207 20 0.4885903547484389 2207 21 0.9202207929510351 2207 22 0.6974654418860098 2207 23 0.6442941964738793 2207 24 0.7735145385242461 2207 25 0.9142454569631084 2208 1 0.21090826806117136 2208 2 0.19062328785836158 2208 3 0.18700584782642693 2208 4 0.156487357599282 2208 5 0.11175787305460005 2208 6 0.2715162416562933 2208 7 0.29408425985641173 2208 8 0.2139699281504619 2208 9 0.37428129274558586 2208 10 0.3598202075415955 2208 11 -0.04154112360229334 2208 12 -0.19098007275976414 2208 13 0.06268167047640105 2208 14 -0.4353635855108351 2208 15 0.17577380298925505 2208 16 0.2506530307325434 2208 17 0.2065344071830424 2208 18 0.3057594847630014 2208 19 0.5351882554764984 2208 20 0.46258963853632906 2208 21 0.7611623888709858 2208 22 0.7291389252478524 2208 23 0.738902995126459 2208 24 0.7089713270606595 2208 25 0.7758582590576992 2209 1 -9.143142540075644E-4 2209 2 0.03230350115303793 2209 3 0.10961534254582972 2209 4 -0.4540551977952063 2209 5 -0.048540584182923396 2209 6 0.1397591490905441 2209 7 0.15425846776695096 2209 8 0.2083442810185474 2209 9 0.3159529638990364 2209 10 0.30513574906158425 2209 11 0.37995918702102216 2209 12 0.3774411842292622 2209 13 0.47152252779675763 2209 14 0.41995661535891315 2209 15 -0.18622531691134725 2209 16 -0.26418440973131396 2209 17 0.22344059894116697 2209 18 -0.11487548106843803 2209 19 0.2612627533021339 2209 20 0.34884895723585885 2209 21 0.6619974478894122 2209 22 0.6656845351049654 2209 23 0.6126747387586775 2209 24 0.6345534869281266 2209 25 0.6935909676930265 2210 1 -0.08409118210607858 2210 2 -0.07954576653160572 2210 3 -0.022938388903423146 2210 4 -0.029548220415591686 2210 5 -0.12063450038157697 2210 6 -0.5454525176141445 2210 7 -0.13609842527100113 2210 8 -0.25850555953839915 2210 9 -0.06526705161849833 2210 10 0.17308829831770822 2210 11 0.008271933263195308 2210 12 0.08202959662452258 2210 13 0.14944223493726128 2210 14 0.12599632989164178 2210 15 -0.0034562649342717577 2210 16 0.11530210957089082 2210 17 -0.9376760329900407 2210 18 -1.5419943973119388 2210 19 -0.29128449072070595 2210 20 -0.022202997796618718 2210 21 -0.4425726633503402 2210 22 -0.3093371046016742 2210 23 -0.21491879001425218 2210 24 0.1598423260684001 2210 25 0.17294216353512581 2211 1 -0.2273666935972969 2211 2 -0.25155113966292575 2211 3 -0.1754733287561514 2211 4 -0.05439423317759662 2211 5 -0.07228048169710632 2211 6 -0.058964217384378514 2211 7 -0.11087468826353586 2211 8 -0.06898878198757075 2211 9 0.013343776219066683 2211 10 0.02374366991401189 2211 11 -0.24838574785529488 2211 12 -0.0381213150253501 2211 13 -0.04794858495751697 2211 14 -0.13311908553728102 2211 15 -0.2072815542480413 2211 16 -0.11689152477012499 2211 17 -0.37217731739256266 2211 18 -0.2820873738757399 2211 19 -0.05450640050742095 2211 20 -0.20027744856504626 2211 21 0.10038881544033984 2211 22 0.05748938005773141 2211 23 -0.5991030469699012 2211 24 -0.13105002520546094 2211 25 -0.7371603431951996 2212 1 -0.25425387495169005 2212 2 -0.33516780653884837 2212 3 -0.22825058339223503 2212 4 -0.08943154364335726 2212 5 -0.08892203756236228 2212 6 -0.22704817953403023 2212 7 -0.281745530856476 2212 8 -0.47392493777498146 2212 9 -0.2703017680784825 2212 10 -0.5612804573810095 2212 11 -0.2882110603801422 2212 12 -0.4129238813492577 2212 13 -0.4088623670208733 2212 14 -0.4039053884321362 2212 15 -0.1627276685607768 2212 16 -0.4466218766143015 2212 17 -0.7028137506543288 2212 18 -0.44688140252716835 2212 19 -0.4087584962130606 2212 20 -0.6570498317056561 2212 21 -0.13696329240217864 2212 22 -0.4284125790032931 2212 23 -0.290177831121639 2212 24 -0.4754337852131627 2212 25 -0.27414855367393187 2213 1 -0.2707343595441358 2213 2 -0.3055905131917811 2213 3 -0.22918108472696375 2213 4 -0.18813990550345494 2213 5 -0.16545490137835372 2213 6 -0.41021152299171604 2213 7 -0.49745477031116536 2213 8 -0.4849687929625617 2213 9 -0.3963615460648171 2213 10 -0.41692214095215974 2213 11 -0.16414996106527013 2213 12 -0.07577096634447855 2213 13 -0.03277065598786777 2213 14 -0.7510517532130336 2213 15 -0.35690664369616343 2213 16 -0.4548201221585586 2213 17 -0.3915806808814679 2213 18 -0.2952129008386919 2213 19 -0.4772144431364964 2213 20 -0.5542396248658363 2213 21 -0.1270259793423847 2213 22 -0.5339403247869043 2213 23 -0.6216189621833763 2213 24 -0.7534695756138916 2213 25 -0.6027956559897305 2214 1 -0.31871616854057905 2214 2 -0.24001186394026064 2214 3 -0.20278123015639338 2214 4 -0.12332895331150695 2214 5 -0.10197150444349035 2214 6 -0.1630321893327391 2214 7 -0.12599450959677938 2214 8 -0.09817640657188065 2214 9 -0.1650453931970901 2214 10 -0.08819269746732772 2214 11 0.022114374314192276 2214 12 -0.3021401416431715 2214 13 -0.17378677922170027 2214 14 -0.22439294600066972 2214 15 -0.0743394081681572 2214 16 -0.2131193988958202 2214 17 -0.4601855519971636 2214 18 -0.2350695610742335 2214 19 -0.4243787782848756 2214 20 -0.4346712892636475 2214 21 -0.6137540581057976 2214 22 -0.8750012014636042 2214 23 -1.1909523284554808 2214 24 -0.5703339922692194 2214 25 -0.8866773519636482 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 7.0 9 51.0 10 105.0 11 289.0 12 880.0 13 2117.0 14 4304.0 15 7580.0 16 11249.0 17 14474.0 18 16938.0 19 19164.0 20 20816.0 21 22564.0 22 24648.0 23 27311.0 24 30230.0 25 34142.0 26 38330.0 27 44067.0 28 51861.0 29 63678.0 30 79337.0 31 103438.0 32 136752.0 33 182449.0 34 247017.0 35 340370.0 36 481705.0 37 747480.0 38 1615152.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.13494697186153 22.316383468812646 12.365510710453556 25.183158848872267 2 13.859955783510427 22.633275540882856 40.3371083844225 23.169660291184215 3 16.82661496978593 28.322745496769556 30.755388427979025 24.095251105465486 4 11.236675048025115 18.836925687501473 40.001881856499516 29.924517407973898 5 12.10445202617414 39.127655370316816 35.951731842142806 12.816160761366241 6 28.769201606046163 40.959992161161395 17.941416586194944 12.3293896465975 7 24.33512057100023 34.53013691165515 24.547638378124173 16.587104139220457 8 23.157900038899022 38.38376507279887 21.90122578856378 16.55710909973833 9 24.15093320318956 16.92122379708193 23.110713924361256 35.817129075367255 10 13.794388081545 30.863069410328446 34.69861425713739 20.64392825098916 11 30.97029070674359 26.781702056797226 24.147009703662963 18.100997532796217 12 20.003255159897712 28.370366690786593 33.073363108966134 18.55301504034956 13 25.841918844968177 24.560404330728396 27.89857303520222 21.69910378910121 14 19.5080746843058 23.690158655162406 29.88656435162167 26.915202308910125 15 21.978383209078906 31.11605107159143 25.510523626654358 21.395042092675308 16 20.150294082502423 32.11670728318312 27.332412804206108 20.400585830108355 17 18.738492916913223 30.971762651067124 30.096726454473554 20.193017977546095 18 19.051813591296025 30.38839305233139 31.152273086768943 19.40752026960364 19 19.630343411803675 29.529399995925367 31.521210890635594 19.31904570163537 20 20.91589416586395 28.99615794543477 30.662543813706133 19.425404074995146 21 21.078054005252604 29.566663713708664 29.806746066900292 19.548536214138444 22 19.82816027843776 29.27385092802286 30.955735693815523 19.942253099723857 23 19.709808858876045 29.594895272976995 30.68751287627332 20.00778299187364 24 19.80011468454311 29.014411108605803 30.803306852115313 20.382167354735774 25 20.252369440145294 28.901755936963262 30.09344074322235 20.75243387966909 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2516.0 1 2516.0 2 2845.5 3 3175.0 4 3175.0 5 3175.0 6 7131.0 7 11087.0 8 11087.0 9 11087.0 10 16874.5 11 22662.0 12 22662.0 13 22662.0 14 36057.0 15 49452.0 16 49452.0 17 49452.0 18 80326.0 19 111200.0 20 111200.0 21 111200.0 22 159413.5 23 207627.0 24 207627.0 25 207627.0 26 266605.0 27 325583.0 28 325583.0 29 325583.0 30 383762.0 31 441941.0 32 441941.0 33 441941.0 34 478269.0 35 514597.0 36 514597.0 37 514597.0 38 526133.0 39 537669.0 40 537669.0 41 537669.0 42 521708.5 43 505748.0 44 505748.0 45 505748.0 46 499161.5 47 492575.0 48 492575.0 49 492575.0 50 449793.5 51 407012.0 52 407012.0 53 407012.0 54 352860.5 55 298709.0 56 298709.0 57 298709.0 58 252030.0 59 205351.0 60 205351.0 61 205351.0 62 166762.5 63 128174.0 64 128174.0 65 128174.0 66 95741.0 67 63308.0 68 63308.0 69 63308.0 70 44797.5 71 26287.0 72 26287.0 73 26287.0 74 18065.5 75 9844.0 76 9844.0 77 9844.0 78 6379.5 79 2915.0 80 2915.0 81 2915.0 82 1866.0 83 817.0 84 817.0 85 817.0 86 506.0 87 195.0 88 195.0 89 195.0 90 121.5 91 48.0 92 48.0 93 48.0 94 30.5 95 13.0 96 13.0 97 13.0 98 7.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09442587465829279 2 0.0 3 0.12906014092689813 4 0.010964847020926604 5 0.022639318796860985 6 0.011720254018192947 7 0.019961057624734862 8 0.0180153123287458 9 9.15644845171324E-5 10 0.3968862581395104 11 0.06489632840151759 12 0.0013047939043691367 13 6.86733633878493E-5 14 8.698626029127579E-4 15 0.001144556056464155 16 9.15644845171324E-5 17 2.28911211292831E-5 18 4.3493130145637896E-4 19 4.3493130145637896E-4 20 2.2891121129283101E-4 21 1.1445560564641551E-4 22 0.0030445191101946523 23 1.373467267756986E-4 24 2.28911211292831E-5 25 0.001579487357920534 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4368506.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.06079297420492 #Duplication Level Percentage of deduplicated Percentage of total 1 89.3798431479962 70.66441275190635 2 8.208951517070021 12.980124328527165 3 1.370214756219996 3.2499079551512926 4 0.37710474535897986 1.1925680080966627 5 0.17617669208634443 0.6964334489959362 6 0.09817511134653258 0.4657081292032637 7 0.06420107477335718 0.35530515169644894 8 0.04981269699448252 0.3150585059654066 9 0.03732175151842136 0.2655618543209366 >10 0.20801584861968483 3.0191920667643 >50 0.017144589167433073 0.9351361815072351 >100 0.010744976280214728 1.5926402839544396 >500 0.0012191128596708613 0.6657381663052253 >1k 8.998208368432011E-4 1.6395142692715026 >5k 2.9026478607823074E-5 0.20020327373038205 >10k+ 1.4513239303911538E-4 1.7624956246034849 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 23968 0.5486543912266574 No Hit GGTATCAACGCAGAGTACTTTTTTT 15870 0.3632820923217228 No Hit TATCAACGCAGAGTACTTTTTTTTT 14291 0.32713701205858475 No Hit CCCATGTACTCTGCGTTGATACCAC 12251 0.28043912495484724 No Hit GTACATGGGAAGCAGTGGTATCAAC 10422 0.2385712644093885 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 8724 0.1997021407318658 No Hit GAGTACATGGGAAGCAGTGGTATCA 4958 0.11349417855898562 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 4659 0.10664973334132996 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.5782242258566204E-5 0.0 10 0.0 0.0 0.0 4.5782242258566204E-5 0.0 11 0.0 0.0 0.0 6.86733633878493E-5 0.0 12 0.0 0.0 0.0 6.86733633878493E-5 0.0 13 0.0 0.0 0.0 1.1445560564641551E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGGCG 60 0.005787456 11.106609 4 AGTACCG 70 0.0014664456 10.879945 5 CCGTGCA 90 9.324949E-5 10.577481 9 TATACCG 75 0.002603502 10.154614 5 GTCCTAC 235 0.0 10.112736 1 CGCGATC 125 2.630566E-6 9.862901 17 GGCACCG 80 0.004440688 9.519951 5 CGACTAA 230 4.3655746E-11 9.07123 17 TACGGAG 95 0.0017903264 9.018902 5 CCTACAC 460 0.0 8.878784 3 CTAGACT 440 0.0 8.870864 4 TATACTG 1155 0.0 8.819349 5 TCTAGAC 485 0.0 8.812795 3 ACCGTAT 130 4.2661006E-5 8.787647 8 CTTAGAC 400 0.0 8.785867 3 TAGACAG 710 0.0 8.715448 5 CTAGGAC 470 0.0 8.689875 3 GCGACTA 230 4.1836756E-10 8.658901 16 CGCCTCC 285 0.0 8.657316 11 GCGAACC 110 7.27309E-4 8.621416 18 >>END_MODULE