FastQCFastQC Report
Wed 25 May 2016
SRR1294680_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294680_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810733
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74610.9202783160423962No Hit
GGTATCAACGCAGAGTACTTTTTTT51780.6386812921146666No Hit
TATCAACGCAGAGTACTTTTTTTTT44300.5464191046867464No Hit
GTACTGGTTCACTATCGGTCAGTCA31500.3885372866282734No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA26970.33266192445601694No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20990.2589015125818241No Hit
ATACAGGGTGACAGCCCCGTACACA19750.2436067114574095No Hit
GGATACCACGTGTCCCGCCCTACTC19180.23657603674699315No Hit
GTTAATGATAGTGTGTCGAAACACA18800.23188892027338226No Hit
GGTATCAACGCAGAGTACATGGGGG18480.2279418748219204No Hit
ACTTAGATGTTTCAGTTCCCCCGGT14860.1832909231522585No Hit
ACCCTGTATCGCGCGCCTTTCCAGA13040.16084210214706937No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12210.15060445300734027No Hit
GTATCAACGCAGAGTACATGGGGGT11900.14678075272623664No Hit
GTTCACTATCGGTCAGTCAGGAGTA11730.14468388483014752No Hit
GTATTTAGCCTTGGAGGATGGTCCC11080.1366664487568657No Hit
AGCGTACACGGTGGATGCCCTGGCA11010.1358030325643584No Hit
GTGGATGCCCTGGCAGTCAGAGGCG10790.13308943881647842No Hit
GTACATGGGGGTTAAGCGACTAAGC10520.12975911921680752No Hit
GTACTTAGATGTTTCAGTTCCCCCG10370.1279089416614348No Hit
GGTATCAACGCAGAGTACATGGGGA10370.1279089416614348No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10340.12753890615036023No Hit
TCATTAACCTATGGATTCAGTTAAT9880.12186502831388385No Hit
CCCATATTCAGACAGGATACCACGT9610.11853470871421294No Hit
ATCATTAACTGAATCCATAGGTTAA9480.11693122149955656No Hit
GCCTTGGAGGATGGTCCCCCCATAT9410.1160678053070493No Hit
GCCCAGAGCCTGAATCAGTGTGTGT9070.11187406951487111No Hit
GTGGTATCAACGCAGAGTACATGGG8830.10891378542627474No Hit
GGGTTTCCCCATTCGGAAATCGCCG8680.107063607870902No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG8660.10681691753018564No Hit
GTCAGGAGTATTTAGCCTTGGAGGA8630.10644688201911111No Hit
GGGTACTTAGATGTTTCAGTTCCCC8260.10188311071585837No Hit
GGTTAATGAGGCGAACCGGGGGAAC8230.10151307520478382No Hit
CTATCGGTCAGTCAGGAGTATTTAG8170.10077300418263474No Hit
ACGCAGAGTACATGGGGGTTAAGCG8150.10052631384191836No Hit
GCAGAGTACTTTTTTTTTTTTTTTT8120.10015627833084381No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCAC456.772049E-414.7735533
GCTCCGT456.772049E-414.7735536
ACAGATC400.005286234414.2459258
GGTATCA15900.013.0843961
TATGCTG604.0981403E-412.6630455
TCTACTC604.0981403E-412.6630453
TAGACAG701.09257E-412.21079355
ATCGCCG1800.012.13541919
GTCTTTA550.003067159812.0904371
AGGGTGA4950.011.8955885
TTCTACT802.8707802E-511.8716042
AATCGCC1850.011.81618718
GGGTGAC4900.011.6293276
ATACAGG5800.011.6288571
TCCGAAT2050.011.59063917
ACAGGGT4800.011.4758843
TACAGGG5350.011.3612372
GCGATTT1604.5474735E-1111.27802411
GGTGACA4750.011.1967977
CGAATGG2150.011.04335319
Produced by FastQC (version 0.11.5)