##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294680_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 810733 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.216647157572222 31.0 29.0 33.0 22.0 34.0 2 29.240646427368812 31.0 29.0 34.0 22.0 34.0 3 29.252113827857013 31.0 28.0 34.0 20.0 34.0 4 32.53154984440007 35.0 33.0 37.0 19.0 37.0 5 33.34908903424432 35.0 33.0 37.0 27.0 37.0 6 33.28802330730339 36.0 33.0 37.0 26.0 37.0 7 33.21265324095602 36.0 33.0 37.0 25.0 37.0 8 32.95422290692497 35.0 33.0 37.0 23.0 37.0 9 33.75635628499148 38.0 33.0 39.0 17.0 39.0 10 34.03645713200277 38.0 33.0 39.0 19.0 39.0 11 34.254128054488966 38.0 34.0 39.0 23.0 39.0 12 34.10190407939482 38.0 33.0 39.0 22.0 39.0 13 33.85441569542624 38.0 33.0 39.0 18.0 39.0 14 34.64884493415218 39.0 33.0 41.0 17.0 41.0 15 34.66203670012199 38.0 33.0 41.0 18.0 41.0 16 34.83448064899295 38.0 33.0 41.0 20.0 41.0 17 34.92809223258459 39.0 33.0 41.0 22.0 41.0 18 34.85382363860852 38.0 33.0 41.0 21.0 41.0 19 35.18736254722578 39.0 33.0 41.0 23.0 41.0 20 35.30547541545737 39.0 34.0 41.0 24.0 41.0 21 35.3890639705057 39.0 34.0 41.0 24.0 41.0 22 35.46624474395393 39.0 34.0 41.0 25.0 41.0 23 35.49395793683001 39.0 34.0 41.0 25.0 41.0 24 35.54486002173342 39.0 33.0 41.0 25.0 41.0 25 34.490012124830244 38.0 32.0 40.0 23.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1208967732239046 1101 2 1.2103387682726563 1101 3 1.561402179916172 1101 4 1.436158745458549 1101 5 0.9692707733417052 1101 6 1.0824645471704528 1101 7 0.9330006304803078 1101 8 1.1105065331762631 1101 9 1.3972382049958014 1101 10 1.0702350653793786 1101 11 1.1053506660871193 1101 12 1.1991809530959472 1101 13 1.4065964987942507 1101 14 1.483743293226162 1101 15 1.6429644006897206 1101 16 1.5485313699110606 1101 17 1.5371660895542547 1101 18 1.3599060779335375 1101 19 1.437189229309972 1101 20 1.211664099226283 1101 21 1.12522195461478 1101 22 1.0197866054376803 1101 23 1.1151023216186005 1101 24 1.1851848683282213 1101 25 1.3121606594891233 1102 1 0.9286970146566063 1102 2 1.0016409284258962 1102 3 1.1337612667909127 1102 4 1.1120213058977058 1102 5 0.9531554643920686 1102 6 0.6482587376690603 1102 7 0.7185973774013874 1102 8 0.8306691871220053 1102 9 1.200988294075394 1102 10 0.9973755131312245 1102 11 0.9629172490369555 1102 12 1.0270392204383967 1102 13 1.1856213309466952 1102 14 1.3674431306154062 1102 15 1.3382738470141362 1102 16 1.2874136963030196 1102 17 1.2024881042000217 1102 18 1.3606516471683605 1102 19 0.9636177961110377 1102 20 0.3096791898499234 1102 21 0.6273258527379539 1102 22 0.5791427705016332 1102 23 0.6962074053102256 1102 24 0.7328640478889383 1102 25 0.9574678383328816 1103 1 0.6170564210833227 1103 2 0.7721721662332754 1103 3 0.9783429799025356 1103 4 0.7257777569593671 1103 5 0.6193012404904508 1103 6 0.2918047523017151 1103 7 0.32071746922599687 1103 8 0.39109618968578985 1103 9 0.5984225887710792 1103 10 0.3114624440540794 1103 11 0.5999454103974315 1103 12 0.7091605422368588 1103 13 0.45720712011102904 1103 14 0.44731481006405005 1103 15 0.4885907669176319 1103 16 0.642875133093959 1103 17 0.5894353594931943 1103 18 0.5922713513495665 1103 19 0.69701541742225 1103 20 -0.16014057865286446 1103 21 0.22813380441812825 1103 22 -0.1286224980611408 1103 23 0.4850299674667582 1103 24 0.39468273562781064 1103 25 0.5685289327474905 1104 1 0.26421630442943567 1104 2 0.22365775132850985 1104 3 0.49424804655135546 1104 4 0.3605035484725292 1104 5 0.2429861529458961 1104 6 -0.05392510713684828 1104 7 0.005570377256233883 1104 8 0.1854385942401393 1104 9 0.4395052888184239 1104 10 0.4522827417518087 1104 11 0.39515002222206874 1104 12 0.1518577155552805 1104 13 0.23530341370244656 1104 14 0.16678839118683442 1104 15 0.5387395370419483 1104 16 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2207 11 0.23266513943607237 2207 12 0.45543925071638824 2207 13 0.5648336771147839 2207 14 0.9238270501013304 2207 15 0.8394783943332413 2207 16 0.9314709833497048 2207 17 0.8894698825605971 2207 18 0.8871256255467941 2207 19 -0.34678192322830625 2207 20 0.3287230489702395 2207 21 -0.2601243279554737 2207 22 0.13670426813809655 2207 23 -0.40481819364327976 2207 24 -1.7585191894356669 2207 25 -0.2161374590464007 2208 1 0.6899462963564211 2208 2 0.4300754544879304 2208 3 0.8976844647334232 2208 4 0.7655768042228388 2208 5 0.6876616417736656 2208 6 0.6819513962907635 2208 7 0.7235917536365122 2208 8 0.8756807675225673 2208 9 0.2831898666272181 2208 10 0.23655370763990646 2208 11 0.7850367776918787 2208 12 0.819634421492367 2208 13 1.0344769561131315 2208 14 1.3381321031307891 2208 15 1.238906213970921 2208 16 0.8511496982091415 2208 17 0.9173697949034647 2208 18 0.9440095078251289 2208 19 -0.8833838261962157 2208 20 0.44056049162009714 2208 21 0.2574323538802261 2208 22 0.7283494867835714 2208 23 0.9068060121582562 2208 24 1.0123055957732774 2208 25 1.0613739021880164 2209 1 0.08685494083741574 2209 2 -1.626025897415115 2209 3 -0.28547674591383654 2209 4 0.15214387156137832 2209 5 0.16182537776181505 2209 6 0.24848435986286432 2209 7 0.25804362927076596 2209 8 0.30154517188038454 2209 9 -2.027339580130665 2209 10 -0.2621559189829057 2209 11 0.0778323388074611 2209 12 0.3113920704174973 2209 13 0.38765142042592515 2209 14 0.6136239030631288 2209 15 0.5775464812021482 2209 16 0.45039617847882596 2209 17 0.4283849906902901 2209 18 0.6802659378212823 2209 19 0.3912488183547609 2209 20 0.25655762217964195 2209 21 0.27645952899774784 2209 22 0.23168841902511872 2209 23 0.6026919810609854 2209 24 0.6042253797487334 2209 25 0.6859711161479609 2210 1 -0.06299706848951558 2210 2 -0.1254436281451241 2210 3 -0.10900102196125516 2210 4 -0.12283793667351972 2210 5 -0.2152620276941164 2210 6 -0.2284018074907479 2210 7 -0.34035257409457387 2210 8 -0.23076180950824465 2210 9 -0.15207799051563597 2210 10 -1.9071569016037913 2210 11 -0.8380921613837486 2210 12 -2.3737632500762587 2210 13 -0.8706022630745451 2210 14 -0.6915203828636152 2210 15 -0.8017428581579296 2210 16 -0.510271432743238 2210 17 -0.3877772639200643 2210 18 0.06969884606042598 2210 19 -0.015692999865684953 2210 20 -0.009380422245449438 2210 21 -0.15120297525142945 2210 22 -0.046773062236034946 2210 23 -0.027203529158271067 2210 24 0.07682984297215967 2210 25 0.007058425997534812 2211 1 -0.3943843324468226 2211 2 -0.136709535370084 2211 3 -1.4004688483159349 2211 4 -0.5212343857703985 2211 5 -0.42501161718508484 2211 6 -0.36623149755643425 2211 7 -0.20587494683677932 2211 8 -0.03134678487770515 2211 9 -0.05416645521186325 2211 10 0.24725350480014896 2211 11 -0.25047285719655576 2211 12 -0.02149133915671797 2211 13 -0.1575108735014723 2211 14 -0.529895280236353 2211 15 -0.41938063525152813 2211 16 0.039655445335583295 2211 17 0.059708368263841294 2211 18 0.09404331445123404 2211 19 0.0035034287057484903 2211 20 -0.27083234137516854 2211 21 -0.21806206802646955 2211 22 -0.23808053350040126 2211 23 -0.4905232643799451 2211 24 -0.25819812295559075 2211 25 -0.03611172999589485 2212 1 -0.15080697533949916 2212 2 -0.38185786097948693 2212 3 -2.4220107539007287 2212 4 -1.292712143474688 2212 5 -0.530080608885136 2212 6 -0.3141041443765289 2212 7 -0.25239394246424496 2212 8 -0.2091725644708191 2212 9 -0.913848857746359 2212 10 -0.28265773547948214 2212 11 -0.25200847890734224 2212 12 -0.029292506909968097 2212 13 -0.10421838534919203 2212 14 0.14241378163362128 2212 15 0.37176302981799125 2212 16 -0.8434212964757464 2212 17 -1.2009179245123178 2212 18 -0.1961486055341055 2212 19 -0.09114349612341499 2212 20 -0.17410519242117317 2212 21 0.10681290906357788 2212 22 -0.18935563626792629 2212 23 0.01863548198718945 2212 24 0.0636645220317078 2212 25 0.045167562020971275 2213 1 -3.458671722279604 2213 2 -1.3400254430139285 2213 3 -2.560793569337349 2213 4 -1.3156631917755561 2213 5 -1.1109252930002427 2213 6 -0.7297297047210733 2213 7 -0.44755920674764127 2213 8 -0.3956123925986219 2213 9 -0.4124369555302181 2213 10 -0.6524147368807576 2213 11 -0.684568564444973 2213 12 -0.2669187712132839 2213 13 -0.8693016982057387 2213 14 -0.461541337674106 2213 15 -0.5191695163211918 2213 16 -2.0067706127723923 2213 17 -1.138269246427356 2213 18 -0.4002196123943875 2213 19 -0.2722983205653904 2213 20 -0.2590364900297004 2213 21 -0.212851116650846 2213 22 -0.13757116719230567 2213 23 -0.06727504883757263 2213 24 0.02723299530451584 2213 25 -0.18225462064969378 2214 1 -1.060613704477067 2214 2 -0.999695231682395 2214 3 -6.549695642995111 2214 4 -2.336574510607022 2214 5 -1.6320773457448254 2214 6 -0.8132938059346557 2214 7 -0.8282050676424717 2214 8 -0.6113552131821436 2214 9 -0.5372139590901028 2214 10 -0.46589831461426456 2214 11 -1.006215097659748 2214 12 -0.5593246541676535 2214 13 -5.241178896025811 2214 14 -3.4818875359682124 2214 15 -2.142705051683052 2214 16 -2.472265108733822 2214 17 -3.415307816523679 2214 18 -1.1728248986260752 2214 19 -0.8798121929733185 2214 20 -0.43943263209060035 2214 21 -0.43610718999258324 2214 22 -0.42539288157716015 2214 23 -0.2388046231692016 2214 24 -0.1453828249722804 2214 25 -0.23566160967879313 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 281.0 3 50.0 4 38.0 5 86.0 6 10.0 7 19.0 8 30.0 9 69.0 10 217.0 11 625.0 12 1545.0 13 2975.0 14 5099.0 15 7619.0 16 9971.0 17 11418.0 18 11628.0 19 10838.0 20 10256.0 21 9485.0 22 8722.0 23 8089.0 24 7973.0 25 7911.0 26 8386.0 27 8830.0 28 10331.0 29 12100.0 30 15182.0 31 18991.0 32 25448.0 33 33936.0 34 46425.0 35 63718.0 36 89625.0 37 134411.0 38 228396.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.1284001437163 20.170163063675954 12.795936433934333 21.905500358673414 2 17.383679745676325 22.549135758589962 36.24643036087601 23.820754134857697 3 19.89393881427198 27.71585870191673 27.328588512465334 25.061613971345952 4 13.367344042510917 20.025964634292322 32.42629881789338 34.180392505303374 5 13.775320692782792 40.22446952261663 30.60066267253241 15.399547112068168 6 30.09911133053567 37.97889410022216 16.3961984695137 15.525796099728462 7 26.985984192103952 36.39321277467577 18.208208059953495 18.412594973266785 8 25.387091280233598 36.30722446974727 18.791297758536 19.514386491483126 9 25.15511200030609 20.155982133269813 18.82177825556237 35.86712761086173 10 17.62984484403561 31.83517502101289 27.440426453653778 23.09455368129773 11 31.520604243030103 28.952324533797373 19.76600392461772 19.761067298554803 12 21.826975766042843 30.499661840280005 26.215153504766324 21.45820888891083 13 27.88920195441749 26.09991521313345 23.66553247262939 22.345350359819665 14 21.66845833235625 27.197361746559324 24.760655268299367 26.373524652785065 15 24.985867861418644 30.96481159205638 22.391355327631107 21.657965218893867 16 22.02332834315941 31.205824310498247 23.512605228698476 23.258242117643864 17 21.222959871937753 30.618675414789116 24.557070761301407 23.601293951971723 18 21.689675959324443 28.55960510322064 26.76399086875315 22.986728068701762 19 22.72699221430652 29.308421089009684 26.932969968057428 21.03161672862637 20 24.037144274701756 26.59784806095926 27.409576844563254 21.955430819775724 21 24.92632067414732 26.95095943564352 26.400030607157138 21.722689283052027 22 24.742134436916892 26.974151114095253 25.81185842171588 22.471856027271976 23 24.462801553154566 27.105904307708258 26.38745923956644 22.043834899570736 24 23.970237169469893 26.978366648645046 26.12505523750275 22.926340944382314 25 23.668408261404604 27.605572609442135 26.975586816214236 21.750432312939026 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3387.0 1 3387.0 2 3292.5 3 3198.0 4 3198.0 5 3198.0 6 3811.5 7 4425.0 8 4425.0 9 4425.0 10 5057.0 11 5689.0 12 5689.0 13 5689.0 14 6607.0 15 7525.0 16 7525.0 17 7525.0 18 10685.5 19 13846.0 20 13846.0 21 13846.0 22 18193.0 23 22540.0 24 22540.0 25 22540.0 26 29727.5 27 36915.0 28 36915.0 29 36915.0 30 43760.5 31 50606.0 32 50606.0 33 50606.0 34 57288.5 35 63971.0 36 63971.0 37 63971.0 38 64861.5 39 65752.0 40 65752.0 41 65752.0 42 72969.5 43 80187.0 44 80187.0 45 80187.0 46 92302.5 47 104418.0 48 104418.0 49 104418.0 50 101976.5 51 99535.0 52 99535.0 53 99535.0 54 91458.5 55 83382.0 56 83382.0 57 83382.0 58 73441.5 59 63501.0 60 63501.0 61 63501.0 62 56600.5 63 49700.0 64 49700.0 65 49700.0 66 38171.0 67 26642.0 68 26642.0 69 26642.0 70 20678.5 71 14715.0 72 14715.0 73 14715.0 74 10806.0 75 6897.0 76 6897.0 77 6897.0 78 4751.5 79 2606.0 80 2606.0 81 2606.0 82 1731.5 83 857.0 84 857.0 85 857.0 86 577.5 87 298.0 88 298.0 89 298.0 90 204.5 91 111.0 92 111.0 93 111.0 94 64.5 95 18.0 96 18.0 97 18.0 98 15.0 99 12.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09916951696797836 2 0.05266838774294373 3 0.05501194597974919 4 0.049338068143272816 5 0.046624474395392815 6 0.06574297580091103 7 0.06228931103088193 8 0.05661543319440556 9 0.0631527272233892 10 0.06364610790482193 11 0.05710881387583828 12 0.057355504216554644 13 0.057478849386912834 14 0.06167258517909102 15 0.0645095240973292 16 0.05846561074977828 17 0.06191927551980739 18 0.14863093028160934 19 0.0652495951194783 20 0.06006909796443465 21 0.057355504216554644 22 0.0646328692676874 23 0.06302938205303102 24 0.07339037636311832 25 0.06857991471914922 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 810733.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.05305857597177 #Duplication Level Percentage of deduplicated Percentage of total 1 82.46063120071372 46.22170590905211 2 10.541081432545338 11.817197099851045 3 3.1036381513404785 5.219052332871256 4 1.3084651417589628 2.9337389294252936 5 0.6890962549102746 1.9312976370484194 6 0.39893540127067717 1.3416929649272444 7 0.2556033896283153 1.0029146239737026 8 0.19331856288219015 0.8668877383246469 9 0.1396303059713535 0.7044035147633825 >10 0.7470861316575624 7.794908302541799 >50 0.08036929878660032 3.1716247613239372 >100 0.06837993618747307 7.666555806180235 >500 0.008880511191676335 3.5465567298791933 >1k 0.004440255595838168 4.2086038825146295 >5k 4.4402555958381675E-4 1.5728597673231735 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7461 0.9202783160423962 No Hit GGTATCAACGCAGAGTACTTTTTTT 5178 0.6386812921146666 No Hit TATCAACGCAGAGTACTTTTTTTTT 4430 0.5464191046867464 No Hit GTACTGGTTCACTATCGGTCAGTCA 3150 0.3885372866282734 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 2697 0.33266192445601694 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2099 0.2589015125818241 No Hit ATACAGGGTGACAGCCCCGTACACA 1975 0.2436067114574095 No Hit GGATACCACGTGTCCCGCCCTACTC 1918 0.23657603674699315 No Hit GTTAATGATAGTGTGTCGAAACACA 1880 0.23188892027338226 No Hit GGTATCAACGCAGAGTACATGGGGG 1848 0.2279418748219204 No Hit ACTTAGATGTTTCAGTTCCCCCGGT 1486 0.1832909231522585 No Hit ACCCTGTATCGCGCGCCTTTCCAGA 1304 0.16084210214706937 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1221 0.15060445300734027 No Hit GTATCAACGCAGAGTACATGGGGGT 1190 0.14678075272623664 No Hit GTTCACTATCGGTCAGTCAGGAGTA 1173 0.14468388483014752 No Hit GTATTTAGCCTTGGAGGATGGTCCC 1108 0.1366664487568657 No Hit AGCGTACACGGTGGATGCCCTGGCA 1101 0.1358030325643584 No Hit GTGGATGCCCTGGCAGTCAGAGGCG 1079 0.13308943881647842 No Hit GTACATGGGGGTTAAGCGACTAAGC 1052 0.12975911921680752 No Hit GTACTTAGATGTTTCAGTTCCCCCG 1037 0.1279089416614348 No Hit GGTATCAACGCAGAGTACATGGGGA 1037 0.1279089416614348 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1034 0.12753890615036023 No Hit TCATTAACCTATGGATTCAGTTAAT 988 0.12186502831388385 No Hit CCCATATTCAGACAGGATACCACGT 961 0.11853470871421294 No Hit ATCATTAACTGAATCCATAGGTTAA 948 0.11693122149955656 No Hit GCCTTGGAGGATGGTCCCCCCATAT 941 0.1160678053070493 No Hit GCCCAGAGCCTGAATCAGTGTGTGT 907 0.11187406951487111 No Hit GTGGTATCAACGCAGAGTACATGGG 883 0.10891378542627474 No Hit GGGTTTCCCCATTCGGAAATCGCCG 868 0.107063607870902 No Hit GTGTGTGTGTTAGTGGAAGCGTCTG 866 0.10681691753018564 No Hit GTCAGGAGTATTTAGCCTTGGAGGA 863 0.10644688201911111 No Hit GGGTACTTAGATGTTTCAGTTCCCC 826 0.10188311071585837 No Hit GGTTAATGAGGCGAACCGGGGGAAC 823 0.10151307520478382 No Hit CTATCGGTCAGTCAGGAGTATTTAG 817 0.10077300418263474 No Hit ACGCAGAGTACATGGGGGTTAAGCG 815 0.10052631384191836 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 812 0.10015627833084381 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAGCAC 45 6.772049E-4 14.773553 3 GCTCCGT 45 6.772049E-4 14.773553 6 ACAGATC 40 0.0052862344 14.245925 8 GGTATCA 1590 0.0 13.084396 1 TATGCTG 60 4.0981403E-4 12.663045 5 TCTACTC 60 4.0981403E-4 12.663045 3 TAGACAG 70 1.09257E-4 12.2107935 5 ATCGCCG 180 0.0 12.135419 19 GTCTTTA 55 0.0030671598 12.090437 1 AGGGTGA 495 0.0 11.895588 5 TTCTACT 80 2.8707802E-5 11.871604 2 AATCGCC 185 0.0 11.816187 18 GGGTGAC 490 0.0 11.629327 6 ATACAGG 580 0.0 11.628857 1 TCCGAAT 205 0.0 11.590639 17 ACAGGGT 480 0.0 11.475884 3 TACAGGG 535 0.0 11.361237 2 GCGATTT 160 4.5474735E-11 11.278024 11 GGTGACA 475 0.0 11.196797 7 CGAATGG 215 0.0 11.043353 19 >>END_MODULE