FastQCFastQC Report
Wed 25 May 2016
SRR1294680_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294680_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810733
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT210892.6012262976837013No Hit
GGTATCAACGCAGAGTACTTTTTTT156111.9255414544615799No Hit
TATCAACGCAGAGTACTTTTTTTTT136181.6797145299377232No Hit
ACGCAGAGTACTTTTTTTTTTTTTT82731.02043459437324No Hit
GTACTTTTTTTTTTTTTTTTTTTTT40640.5012747723356519No Hit
GAGTACTTTTTTTTTTTTTTTTTTT34910.4305979897204135No Hit
GTACTGGTTCACTATCGGTCAGTCA29760.36707522698594974No Hit
GCAGAGTACTTTTTTTTTTTTTTTT27560.3399392895071497No Hit
ATCAACGCAGAGTACTTTTTTTTTT22950.28307716597202776No Hit
ATACAGGGTGACAGCCCCGTACACA22560.2782667043280587No Hit
GGATACCACGTGTCCCGCCCTACTC19650.24237325975382773No Hit
GGTATCAACGCAGAGTACATGGGGG19600.24175653390203683No Hit
GTTAATGATAGTGTGTCGAAACACA18690.23053212339944223No Hit
GTGGTATCAACGCAGAGTACTTTTT17470.21548401261574401No Hit
ACTTAGATGTTTCAGTTCCCCCGGT15940.19661220155094217No Hit
ACCCTGTATCGCGCGCCTTTCCAGA14490.17872715184900578No Hit
AGCGTACACGGTGGATGCCCTGGCA13870.17107975128679848No Hit
TCATTAACCTATGGATTCAGTTAAT10870.13407620017934388No Hit
CCCATATTCAGACAGGATACCACGT10750.13259605813504569No Hit
GTATCAACGCAGAGTACATGGGGGT10570.13037584506859842No Hit
GTACTTAGATGTTTCAGTTCCCCCG10330.12741556098000206No Hit
GTGGATGCCCTGGCAGTCAGAGGCG10300.1270455254689275No Hit
CTATCGGTCAGTCAGGAGTATTTAG10270.12667548995785297No Hit
GTTCACTATCGGTCAGTCAGGAGTA10180.1255653834246293No Hit
GCCTTGGAGGATGGTCCCCCCATAT10170.12544203825427114No Hit
CAACGCAGAGTACTTTTTTTTTTTT9910.12223506382495838No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA9850.1214949928028093No Hit
GTCAGGAGTATTTAGCCTTGGAGGA9720.11989150558815295No Hit
GTACATGGGGGTTAAGCGACTAAGC9660.11915143456600384No Hit
ACGCAGAGTACATGGGGGTTAAGCG9650.11902808939564567No Hit
GCCCAGAGCCTGAATCAGTGTGTGT9550.11779463769206384No Hit
GGTATCAACGCAGAGTACATGGGGA9390.11582111496633293No Hit
GTGGTATCAACGCAGAGTACATGGG9300.11471100843310929No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG9100.11224410502594566No Hit
GTATTTAGCCTTGGAGGATGGTCCC9070.11187406951487111No Hit
CCTATGGATTCAGTTAATGATAGTG8980.11076396298164747No Hit
ATCATTAACTGAATCCATAGGTTAA8700.10731029821161837No Hit
CGGTACTGGTTCACTATCGGTCAGT8690.10718695304126019No Hit
GGGTTTCCCCATTCGGAAATCGCCG8680.107063607870902No Hit
CACACACACTGATTCAGGCTCTGGG8500.10484339480445473No Hit
AACGCAGAGTACTTTTTTTTTTTTT8480.10459670446373837No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAA508.648861E-515.2116781
GGTATCA28200.015.1375071
TGTATAG400.005191494714.2848025
CTATACC551.9182106E-413.851934
TAGAGAG657.853715E-411.7208645
GTGTAGG657.9646404E-411.7012911
TATATCC658.018534E-411.691893
TCTAAGC658.018534E-411.691893
TGTAAGG1001.9264553E-611.3995932
ACAGGGT4850.011.1645493
AGAGTAT600.005765862811.11040210
TCCTAAA600.005873097611.0829362
GTACATA1303.2187017E-810.969961
ATTCTAA700.00148054610.8654841
GTGTAAG1502.0972948E-910.7749381
TCACGGT1951.8189894E-1210.7261831
TAAGGCA803.6824914E-410.7136024
AGGTAAC909.269059E-510.5813366
ACCGGGG2200.010.34217315
CCGGGGG2400.010.27035316
Produced by FastQC (version 0.11.5)