##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294680_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	810733
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.048490686822912	31.0	31.0	34.0	28.0	34.0
2	31.176468701779747	31.0	31.0	34.0	28.0	34.0
3	31.209152705021257	31.0	31.0	34.0	28.0	34.0
4	34.75730110899643	37.0	35.0	37.0	32.0	37.0
5	35.049162918001365	37.0	35.0	37.0	32.0	37.0
6	34.43025015634	37.0	35.0	37.0	30.0	37.0
7	34.28548979750423	37.0	35.0	37.0	30.0	37.0
8	34.30455896084161	37.0	35.0	37.0	30.0	37.0
9	35.619244560169626	39.0	35.0	39.0	29.0	39.0
10	35.44389953289184	39.0	35.0	39.0	28.0	39.0
11	35.59155603632762	39.0	35.0	39.0	29.0	39.0
12	35.59462116381102	39.0	35.0	39.0	29.0	39.0
13	35.3733954335151	39.0	35.0	39.0	28.0	39.0
14	36.27340937151936	39.0	36.0	41.0	27.0	41.0
15	36.295749648774624	39.0	36.0	41.0	27.0	41.0
16	36.35604200149741	39.0	36.0	41.0	27.0	41.0
17	36.517319512095845	39.0	36.0	41.0	28.0	41.0
18	36.57936213278601	39.0	36.0	41.0	29.0	41.0
19	36.6224725032779	39.0	36.0	41.0	28.0	41.0
20	36.70368049653832	39.0	36.0	41.0	29.0	41.0
21	36.52103590207874	39.0	36.0	41.0	28.0	41.0
22	36.539628953058525	39.0	36.0	41.0	28.0	41.0
23	36.536814216270955	39.0	36.0	41.0	28.0	41.0
24	36.43223231322766	39.0	35.0	41.0	28.0	41.0
25	35.26571756669582	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5918901968610157
1101	2	0.5024253043190257
1101	3	0.5306744545462045
1101	4	0.4544902455184996
1101	5	0.40588297654769434
1101	6	0.5205236528274568
1101	7	0.4609094890358705
1101	8	0.5116351549211089
1101	9	0.7871033416646824
1101	10	0.6088402995694366
1101	11	0.838063052942033
1101	12	0.8839434142481934
1101	13	0.9918079434252078
1101	14	1.3094736630652335
1101	15	1.1648485052018458
1101	16	1.1798568610099878
1101	17	1.0850967709781045
1101	18	1.0465902607353783
1101	19	0.9865157341537625
1101	20	0.7823289536282729
1101	21	1.2040067987803482
1101	22	1.2059202654517875
1101	23	1.2670807472551786
1101	24	1.3184116071262437
1101	25	1.1516521930553196
1102	1	0.388214286791456
1102	2	0.32040815352724294
1102	3	0.2389679270092735
1102	4	0.39031244480197813
1102	5	0.40831943850044894
1102	6	0.4548714498080386
1102	7	0.44503172567878835
1102	8	0.43838203281619315
1102	9	0.6178688431342252
1102	10	0.5629562088615714
1102	11	0.6809131465565912
1102	12	0.7472094717903772
1102	13	0.8497108693390558
1102	14	1.019580040300589
1102	15	1.0310266557582395
1102	16	1.154965862044392
1102	17	0.8429589902154078
1102	18	0.9024465154188022
1102	19	1.0280322252166485
1102	20	1.0421201510524156
1102	21	1.305106742849155
1102	22	1.1171505572657523
1102	23	0.9798357578475532
1102	24	0.9710071166507461
1102	25	0.9239415279060594
1103	1	0.3455281448665879
1103	2	0.27501254050121204
1103	3	0.2790509603264404
1103	4	0.1981219722601324
1103	5	0.17943642615333033
1103	6	0.2784206099476094
1103	7	0.06478329112297843
1103	8	0.3499217080426078
1103	9	0.15765950561629438
1103	10	0.2214963105461294
1103	11	0.17415938026574196
1103	12	0.2899863494916559
1103	13	0.47447256190406506
1103	14	0.6158229742519978
1103	15	0.6573414423628208
1103	16	0.5981143531588344
1103	17	0.6033706685147351
1103	18	0.5344438820649344
1103	19	0.6977020780532968
1103	20	0.5386439626405846
1103	21	0.9673377742830525
1103	22	0.8406851996810971
1103	23	0.8779651894080729
1103	24	0.9631998071782348
1103	25	0.7452364080894682
1104	1	0.09214845969035323
1104	2	0.24659857864896395
1104	3	-0.0761794192908205
1104	4	0.009628305448124763
1104	5	0.1492050376748182
1104	6	0.25194612232608904
1104	7	0.25564113814417766
1104	8	0.3289373941125433
1104	9	-0.15965393133282646
1104	10	0.22566364568589847
1104	11	0.2697546428501809
1104	12	0.39944287347745444
1104	13	-0.26216297934261235
1104	14	0.2283090347423382
1104	15	0.49067658016342364
1104	16	0.4695460601422212
1104	17	0.5002832578235612
1104	18	0.661834514123889
1104	19	0.684407255332367
1104	20	0.5814526520363188
1104	21	0.748801695928492
1104	22	0.6686271128954075
1104	23	0.619811375961774
1104	24	0.46098375732768915
1104	25	0.6313374497264022
1105	1	0.038632565857785295
1105	2	0.02030216649216854
1105	3	0.003168464338891397
1105	4	-0.03091552591602209
1105	5	-0.0337263577579634
1105	6	-0.11215475117295881
1105	7	-0.10468901080786708
1105	8	-0.2442050367655284
1105	9	0.014223875053581025
1105	10	0.00674216434701691
1105	11	-0.09997320083439831
1105	12	-0.3652210820535018
1105	13	-0.47509798427559957
1105	14	-0.9244454951804215
1105	15	-0.3562026459597334
1105	16	-0.13110990636161546
1105	17	-0.12882134549236213
1105	18	-0.9276883099490689
1105	19	-0.9282587215187021
1105	20	-0.6562969449028202
1105	21	-0.11336063532737484
1105	22	0.081563561628478
1105	23	0.09819961380377862
1105	24	0.47494145398529497
1105	25	0.1829398271838727
1106	1	-0.4599085482005698
1106	2	-0.28126282024523164
1106	3	-0.49600925449399824
1106	4	-0.4626396638470567
1106	5	-0.301232989057695
1106	6	-0.3869292869819745
1106	7	-0.34423808242590326
1106	8	-0.3529848776143325
1106	9	-0.5399405811798417
1106	10	-1.4370509391012618
1106	11	-1.1084347392959373
1106	12	-1.6750619308598687
1106	13	-0.7285197349387289
1106	14	-0.8559574315199399
1106	15	-0.5490673674451472
1106	16	-0.7249560602077665
1106	17	-1.1515799661820196
1106	18	-0.44140183780052666
1106	19	-0.25547357563011275
1106	20	-0.6275966796508357
1106	21	-0.1975781419586724
1106	22	-0.12018710150150014
1106	23	-0.5815086090078978
1106	24	-0.31235297572293064
1106	25	-0.7687312603492842
1107	1	0.16281428452226265
1107	2	0.07926221027979707
1107	3	0.0036610751763319627
1107	4	0.05101112980373301
1107	5	0.009426351601646843
1107	6	0.06160551155281979
1107	7	-0.04161292980074904
1107	8	-0.1500178446473015
1107	9	0.15603011479085183
1107	10	0.031044298993975872
1107	11	-0.05203669289789303
1107	12	-0.18787023366817124
1107	13	0.14884290242390819
1107	14	0.20230874674623323
1107	15	0.10989478151700638
1107	16	0.18476310951140107
1107	17	0.08016606556401484
1107	18	0.09497178857309763
1107	19	0.1806192204626882
1107	20	0.1273483752942255
1107	21	0.4612863269057925
1107	22	0.5075296262152307
1107	23	0.6410677035684174
1107	24	0.6102233368533803
1107	25	0.5485336969156691
1108	1	0.14576983417341083
1108	2	0.17933758988400683
1108	3	0.2576084669042551
1108	4	0.1842047254895789
1108	5	0.10864874111541667
1108	6	0.1605078821724888
1108	7	0.07376706803430011
1108	8	0.09640851262840755
1108	9	0.13705689127949938
1108	10	-0.506567368115519
1108	11	-0.04478411217971967
1108	12	0.3228850384758175
1108	13	0.3183761589999463
1108	14	0.32010246642464324
1108	15	0.24271049511386877
1108	16	0.24963404900106667
1108	17	0.3768267294162442
1108	18	0.3392548458753737
1108	19	0.3897856165106717
1108	20	0.514144938928979
1108	21	0.7881011942042306
1108	22	0.5565747850929981
1108	23	0.6813456043546893
1108	24	0.866620270497684
1108	25	0.7385261195925352
1109	1	0.05615030819727096
1109	2	0.028167241218724826
1109	3	-0.06290066816507434
1109	4	-0.06951617716424607
1109	5	-0.08498042805937445
1109	6	-0.11602967811626996
1109	7	-0.14817736348382482
1109	8	-0.17193067757708036
1109	9	0.04405271366091057
1109	10	-0.27513810617147527
1109	11	0.15673382095737054
1109	12	-0.05149143356055674
1109	13	0.17208745448202478
1109	14	0.12212042282909863
1109	15	-4.005103873312159
1109	16	-1.823870196918513
1109	17	-1.6623063584338382
1109	18	-0.8630185261968819
1109	19	-0.38595597458708397
1109	20	-0.5384869757953652
1109	21	0.09655670624397317
1109	22	0.15840667760075178
1109	23	-0.016451801408713607
1109	24	0.11275225802637578
1109	25	0.0643717383469351
1110	1	-0.24288636792685736
1110	2	-0.13476447196774544
1110	3	-0.21639151027502734
1110	4	-0.1297562282642346
1110	5	-0.14691019528704885
1110	6	-0.7961742138342629
1110	7	-0.9166543164985796
1110	8	-0.9116304651556248
1110	9	-0.4819132097782344
1110	10	-0.02312455873315855
1110	11	0.005057474012225782
1110	12	-0.09825683411612118
1110	13	-0.0022526278127230626
1110	14	0.0728297322365492
1110	15	-0.03812352601050861
1110	16	-0.05663137875425406
1110	17	-0.03918732814521064
1110	18	-0.26339806243478847
1110	19	0.07484261125049585
1110	20	-0.042046892015157766
1110	21	-0.9841708743796289
1110	22	-1.001300834394371
1110	23	-1.2379078539347503
1110	24	-1.0755006869074464
1110	25	-0.6436817086739666
1111	1	-0.2415610215590327
1111	2	-0.13650364113033575
1111	3	-0.24628102144453123
1111	4	-0.1479633178095483
1111	5	-0.27733192339619706
1111	6	-0.2868310972104666
1111	7	-0.2783369236935229
1111	8	-0.24945609544671044
1111	9	-0.02955107957436809
1111	10	-0.1826831230093049
1111	11	-0.6283337573982237
1111	12	-0.493436448054716
1111	13	-0.1198287767740851
1111	14	-0.29009582790879307
1111	15	-0.6386950839512622
1111	16	-0.46786731712991525
1111	17	-0.6864136019956888
1111	18	-0.9275673178135619
1111	19	-0.6171637426923269
1111	20	-0.4937803084841903
1111	21	-0.17596196624086247
1111	22	-0.2599972369196166
1111	23	-1.4311530782712367
1111	24	-1.2444535853371477
1111	25	-1.6526681582970753
1112	1	-0.19105982869690763
1112	2	-0.18311056013421023
1112	3	-0.09177685257208879
1112	4	-0.0840587056906017
1112	5	-0.0608014921131641
1112	6	0.09622273988745178
1112	7	-0.09938339193749357
1112	8	0.021791910182464846
1112	9	-0.23550992390503467
1112	10	0.0411237122025625
1112	11	0.3266247373099276
1112	12	-0.076594328116947
1112	13	0.21976034156451618
1112	14	-0.6182189419924882
1112	15	-0.15187233082621532
1112	16	-0.07457867531249462
1112	17	0.1015188816045196
1112	18	0.03212850693054037
1112	19	-0.65365802308078
1112	20	-0.47705602564141003
1112	21	-0.28159969348173775
1112	22	-0.57181466132797
1112	23	-0.24173765225088317
1112	24	-0.6480823221799881
1112	25	-0.3123409072215182
1113	1	-0.3295731278070093
1113	2	-0.32832453759764846
1113	3	-0.5467292902721752
1113	4	-0.2862443150098457
1113	5	-0.1946184988966948
1113	6	-0.03653125478162167
1113	7	-0.04320945988565228
1113	8	-0.25313246258750155
1113	9	-0.2843547135590896
1113	10	-0.08658582282218674
1113	11	-3.801775785845507E-4
1113	12	-0.035728299375072936
1113	13	-0.3799776955827312
1113	14	-0.4195978608580475
1113	15	-0.10753785045051245
1113	16	-0.477563394733707
1113	17	-0.5522776406006216
1113	18	-0.23077571973254862
1113	19	-0.23664083859969764
1113	20	-0.37092404995494377
1113	21	-11.128262399561535
1113	22	-3.3978570478342363
1113	23	-2.141208966789648
1113	24	-1.0112975195511922
1113	25	-1.6938564839628825
1201	1	0.4016222971916612
1201	2	0.37099155903884906
1201	3	0.38896408899419654
1201	4	0.3053420574019867
1201	5	0.34261316478846027
1201	6	0.37633803975069213
1201	7	0.5541244461346508
1201	8	0.536131649422984
1201	9	0.6793435010745341
1201	10	0.9596512695811441
1201	11	0.8040785889599675
1201	12	0.8338108794980883
1201	13	0.5865754668891512
1201	14	0.9403868182896531
1201	15	0.7752509874672668
1201	16	0.7897887988935253
1201	17	0.9560514123404502
1201	18	0.6952546740044951
1201	19	0.8563923801962403
1201	20	0.8665845846294431
1201	21	1.0344629852309666
1201	22	0.992943134599038
1201	23	0.9865264252647634
1201	24	1.172437580255746
1201	25	1.198793492125965
1202	1	0.31253139008464004
1202	2	0.3092904087607167
1202	3	0.4684011488562305
1202	4	0.17498211265723995
1202	5	0.3125823073937042
1202	6	0.28418157922917686
1202	7	0.39792634340614796
1202	8	0.21795186472642314
1202	9	0.5961802034796264
1202	10	0.4068172557411458
1202	11	0.6090812404263488
1202	12	0.5523680889770262
1202	13	0.5844139216625521
1202	14	0.6190590992272504
1202	15	0.7001435648889967
1202	16	0.767084936045265
1202	17	0.8358412372563748
1202	18	0.6505796477626191
1202	19	0.9383483331201674
1202	20	0.9017487027604503
1202	21	1.2829332091544003
1202	22	0.9916070355934963
1202	23	1.0475237912565944
1202	24	1.0290003414470092
1202	25	1.0044426431111546
1203	1	0.29422976014826574
1203	2	0.361338120776761
1203	3	0.31719897397738706
1203	4	0.06532619675038376
1203	5	0.15074315712897857
1203	6	0.13315059046632172
1203	7	0.15610659423456497
1203	8	0.12663324253010444
1203	9	0.14298534069344981
1203	10	0.46688931694426117
1203	11	0.43220318607883
1203	12	0.22847784373253432
1203	13	0.4503466618224081
1203	14	0.5842767297668487
1203	15	0.4908155519114601
1203	16	0.3686624976213153
1203	17	0.48201889583789637
1203	18	0.46343348335057755
1203	19	0.46387646266729377
1203	20	0.5401547154689865
1203	21	0.5776718853966187
1203	22	0.608570968309202
1203	23	0.5157657098453683
1203	24	0.7873395508117014
1203	25	0.807379911551358
1204	1	0.05990434161232017
1204	2	0.023317914335500234
1204	3	0.013810565325819368
1204	4	-0.12517604638343727
1204	5	0.09243991461520551
1204	6	-0.021115011167701425
1204	7	0.135353047165232
1204	8	0.14840805377859567
1204	9	-0.33542221666147753
1204	10	-0.016004508054827227
1204	11	-0.07625871711991294
1204	12	0.24500043920249936
1204	13	0.13421068779169332
1204	14	0.18881041324790004
1204	15	0.15372542534764477
1204	16	0.28090672565501507
1204	17	0.09548864088658604
1204	18	0.22299871660002424
1204	19	0.3832513030947666
1204	20	0.21263894058478883
1204	21	0.5920060576255253
1204	22	0.460505899191503
1204	23	0.5301545926553075
1204	24	0.3673628939929401
1204	25	0.3206381090200878
1205	1	0.004284211355010115
1205	2	-0.08019589132066685
1205	3	0.042271007055095566
1205	4	-0.3252243217599826
1205	5	0.025724110511703202
1205	6	-0.09624883405550122
1205	7	-0.21347150967591233
1205	8	-0.37120453632466166
1205	9	-0.1475510218794298
1205	10	-0.05451465713857573
1205	11	-0.4217562001433137
1205	12	-0.4214310765724818
1205	13	-0.05721890032068444
1205	14	-0.5940787435182386
1205	15	-0.15307512886133878
1205	16	0.05317482065981238
1205	17	-0.3553947554010932
1205	18	-0.4882249732000332
1205	19	-0.29112505614683215
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2111	20	-0.11819210533005986
2111	21	0.2228186493319697
2111	22	0.2238868134104024
2111	23	-0.8758250870673905
2111	24	-0.7923305338569122
2111	25	-1.0684992019049346
2112	1	-0.37050297487094497
2112	2	-0.24332569408025861
2112	3	-0.17977472952529538
2112	4	-0.1754305017011859
2112	5	-0.2638225935460312
2112	6	-0.15119738175640407
2112	7	-0.3326718006064553
2112	8	-0.30396600619040015
2112	9	-0.28305367062204567
2112	10	-0.3584433441645487
2112	11	0.019291416404904282
2112	12	0.038474954196004774
2112	13	0.18368316764392034
2112	14	-0.6221557901233652
2112	15	-0.8726649934478345
2112	16	-1.260693993160892
2112	17	-0.7068783386955815
2112	18	-1.3698865598295527
2112	19	-1.101631131154754
2112	20	-0.8372809194616551
2112	21	-0.4828969160116259
2112	22	-0.8604912699069303
2112	23	-0.551417729423342
2112	24	-0.27979072784422243
2112	25	-0.22307168785427223
2113	1	0.009851188284606849
2113	2	-0.02922445558722231
2113	3	0.13347275803587166
2113	4	0.3782671371604991
2113	5	0.08058384230573523
2113	6	0.4425914777277029
2113	7	0.18925348897499106
2113	8	0.38804140928097297
2113	9	0.08647032110008013
2113	10	0.47738231026901445
2113	11	0.5651317529859199
2113	12	0.43774889391568905
2113	13	0.697529480329635
2113	14	0.01581160548838767
2113	15	0.43688690498267846
2113	16	0.5959933681975826
2113	17	0.16516226723527438
2113	18	0.4218759507417431
2113	19	-0.02159900351876587
2113	20	0.580777666406135
2113	21	0.712191928441122
2113	22	-1.6588979456531519
2113	23	-2.2355465861441104
2113	24	-0.9975412467298383
2113	25	-1.682006465412904
2114	1	-0.5355941669296662
2114	2	-0.6818313821181441
2114	3	-0.3745711274036978
2114	4	-0.298492506656423
2114	5	-0.28949385862291166
2114	6	-0.3109761097244501
2114	7	-0.5507598215563334
2114	8	-0.2752925699220228
2114	9	-0.6470977718152966
2114	10	-0.6637733471280143
2114	11	-0.25495647842637226
2114	12	-0.8441254760103689
2114	13	-0.14738261119290996
2114	14	-1.1195025820216173
2114	15	-0.2042847587494947
2114	16	-3.3698390033515793
2114	17	-2.9550582270515875
2114	18	-1.5206994966634042
2114	19	-2.1068448130883084
2114	20	-1.2684662448682218
2114	21	-1.4135982088483061
2114	22	-1.9930299108660492
2114	23	-3.2681641274025424
2114	24	-7.941148962345004
2114	25	-3.976490457673698
2201	1	0.62373001713215
2201	2	0.6083081634936676
2201	3	0.5814893039191027
2201	4	0.4335222551934237
2201	5	0.42651352230200956
2201	6	0.5064959234897088
2201	7	0.5391490701516588
2201	8	0.4379238987667051
2201	9	0.7847785976922594
2201	10	1.0184663022780498
2201	11	0.813340142493935
2201	12	0.9868628759674891
2201	13	0.9658915209717804
2201	14	1.18517969222588
2201	15	1.2330927063641042
2201	16	1.1159065853925085
2201	17	1.0731276799948901
2201	18	1.1274841038440329
2201	19	1.2679331825292692
2201	20	1.3166565783355608
2201	21	1.544480444132894
2201	22	1.4302891988098807
2201	23	1.462097664778291
2201	24	1.6357510491876894
2201	25	1.8169128406771264
2202	1	0.43279101638578865
2202	2	0.472336599203242
2202	3	0.4175030822258847
2202	4	0.34589444355497534
2202	5	0.3143112779960049
2202	6	0.4091665040194741
2202	7	0.45931779565827924
2202	8	0.6328351514132393
2202	9	0.6302480980654437
2202	10	0.937564445514127
2202	11	0.7905347817490522
2202	12	0.8524416975871532
2202	13	0.7811464479350008
2202	14	1.1263357469996507
2202	15	0.9625291492457109
2202	16	1.0407623723031136
2202	17	1.0261558828745834
2202	18	0.9726620228671052
2202	19	0.9000271500592945
2202	20	1.0278169633099807
2202	21	1.1805205518003987
2202	22	1.0519754529252054
2202	23	0.9874173706728016
2202	24	1.27123192993745
2202	25	1.224853645311093
2203	1	0.27987056157854084
2203	2	0.4051207961383838
2203	3	-0.34069678918153556
2203	4	-0.11637703758443507
2203	5	0.11095593313122976
2203	6	0.08313509308240441
2203	7	0.42245633426851725
2203	8	0.23338763875818103
2203	9	0.44191756067829857
2203	10	0.7109577189074017
2203	11	0.5872129463517481
2203	12	0.6125338067358683
2203	13	0.6612527148337222
2203	14	0.7135064379439271
2203	15	0.7285095001317217
2203	16	0.8509102956585863
2203	17	0.7856783310762765
2203	18	0.6405706341719437
2203	19	0.78546770531117
2203	20	0.7952560232018726
2203	21	1.0409400065594738
2203	22	0.8917143303999353
2203	23	1.0342855967863116
2203	24	1.103874130504174
2203	25	1.1805394689214452
2204	1	0.1651725611662549
2204	2	0.1734572216176673
2204	3	0.26651821803347175
2204	4	0.043754893976071685
2204	5	0.055956963846540475
2204	6	0.34514292651880396
2204	7	0.3919471609021983
2204	8	0.3669889580737902
2204	9	0.126642359688816
2204	10	0.45090824457221146
2204	11	0.3835642141315887
2204	12	0.2691819205268402
2204	13	0.4685707935803691
2204	14	0.7300184973131323
2204	15	0.6237269810634913
2204	16	0.5189581208492697
2204	17	0.49114112225335305
2204	18	0.4732030810901051
2204	19	0.627603347446815
2204	20	0.5704776269949008
2204	21	0.8170686398309641
2204	22	0.8435386965100165
2204	23	0.6551266604845196
2204	24	0.7814251509123409
2204	25	0.8953817694780284
2205	1	-0.015285756961546326
2205	2	0.07341604971561821
2205	3	-0.05995063635835152
2205	4	-0.1277840930079961
2205	5	-0.17405173839639332
2205	6	0.19227820428879028
2205	7	0.10315901840998976
2205	8	0.45572002133815914
2205	9	0.39830299092893284
2205	10	0.6100329673636651
2205	11	-0.08885903302147113
2205	12	0.39276687032518964
2205	13	0.0011321537502979595
2205	14	0.04846538088086305
2205	15	0.3577583652815477
2205	16	0.34146230821885126
2205	17	0.40520132680285315
2205	18	0.5045414486241881
2205	19	0.5933530035678558
2205	20	0.5507675146143214
2205	21	0.822640882483185
2205	22	0.9452641734958576
2205	23	0.7650060455260146
2205	24	0.9348494367201994
2205	25	0.8675738925445913
2206	1	-0.41452393281595334
2206	2	-0.39203205101446414
2206	3	-0.1806246391093822
2206	4	0.055821874614480294
2206	5	-0.12661760444230907
2206	6	0.1092245591718708
2206	7	-0.04962269781051276
2206	8	0.03470743007797239
2206	9	0.010828649589392114
2206	10	0.08294906089874132
2206	11	-0.1699732008343986
2206	12	0.01416883837089955
2206	13	-1.266981273400269
2206	14	-0.4790343545968625
2206	15	-0.23137505975283545
2206	16	0.04350547825377049
2206	17	-0.1377338123358669
2206	18	-0.20909414549283412
2206	19	-0.08008896024549728
2206	20	-0.00605821811237206
2206	21	0.26319108881055797
2206	22	0.06904366772926807
2206	23	0.11541446791517984
2206	24	0.16457010120014814
2206	25	-0.0479620295800558
2207	1	0.10419222737135314
2207	2	0.19308330921186467
2207	3	0.16955291320513766
2207	4	0.17873002543639416
2207	5	0.23510621390801134
2207	6	0.1674870829511974
2207	7	0.25989586225972516
2207	8	0.301889030975488
2207	9	0.4105906124204708
2207	10	0.5954347933020401
2207	11	0.4336856450755562
2207	12	0.34998806670122207
2207	13	0.4214944107127039
2207	14	0.31014178315250973
2207	15	0.36551777474304004
2207	16	0.4922635876142891
2207	17	0.6355348087070141
2207	18	0.5557743975050684
2207	19	0.5100383506173927
2207	20	0.42631386129838944
2207	21	0.7297224775233033
2207	22	0.572246438325827
2207	23	0.5019951225173997
2207	24	0.7827550677871429
2207	25	0.6198702712833182
2208	1	0.3210624171086316
2208	2	0.26163499272610835
2208	3	0.28670379226316456
2208	4	0.3284387675254905
2208	5	0.1271154272318995
2208	6	0.3292490689757912
2208	7	0.3610598059602239
2208	8	0.1828427996104054
2208	9	0.3714677898608798
2208	10	0.34093925697717253
2208	11	-0.05874771063832185
2208	12	-0.08169843161401502
2208	13	0.18173856068721506
2208	14	-0.04340087043397034
2208	15	0.4743847441687308
2208	16	0.398277348540347
2208	17	0.29356709264150993
2208	18	0.576309323587104
2208	19	0.7155935435252445
2208	20	0.5760948738936591
2208	21	0.9730685397909511
2208	22	1.01712813228432
2208	23	0.9376071526813945
2208	24	1.144140236946754
2208	25	1.1730353309026
2209	1	-0.23021971829163235
2209	2	-0.11331288202164913
2209	3	0.024128484031603392
2209	4	-0.8158103955543723
2209	5	-0.11636271952948363
2209	6	-0.020079426550914548
2209	7	-0.003893507310358757
2209	8	-0.07694909305386233
2209	9	0.09798190085923864
2209	10	0.15378189849541002
2209	11	0.1651903922649467
2209	12	0.07729014894610486
2209	13	0.2579956176444398
2209	14	0.15637954738455306
2209	15	-0.5104351430365952
2209	16	-0.6455774851837432
2209	17	-0.031508580102304506
2209	18	-0.3551514373978364
2209	19	0.01772827308631264
2209	20	0.129629330507953
2209	21	0.45662356947682525
2209	22	0.4171642918998657
2209	23	0.22891696185194377
2209	24	0.36000001928919545
2209	25	0.31318701174150476
2210	1	-0.1672626003983737
2210	2	-0.1499218174979795
2210	3	-0.08962017482366846
2210	4	-0.0065905567042037205
2210	5	-0.16644418411264184
2210	6	-0.6014450836852774
2210	7	-0.31699735440392374
2210	8	-0.3136339160758723
2210	9	-0.024803218542480465
2210	10	0.03955620375588609
2210	11	0.025964299165600835
2210	12	0.1327076433159533
2210	13	0.09940093813819573
2210	14	0.006001703095442679
2210	15	-0.13494648832426748
2210	16	-0.0017795492187602235
2210	17	-1.0712897876105885
2210	18	-1.6793585685697607
2210	19	-0.43735200969604193
2210	20	-0.1997498389914938
2210	21	-0.584956232618012
2210	22	-0.39016134600699104
2210	23	-0.2827294194474561
2210	24	0.08564496383750964
2210	25	0.04519044294741548
2211	1	-0.12332145713072151
2211	2	-0.1888932358066846
2211	3	0.020869613764180173
2211	4	0.0935672327046646
2211	5	-0.007519461206236144
2211	6	0.10841996147071598
2211	7	0.1733569662036274
2211	8	0.2044156528896437
2211	9	0.19882157620300234
2211	10	0.35582262411713117
2211	11	-0.045605384742444244
2211	12	0.05592834323385176
2211	13	-0.00797154749398743
2211	14	-0.04720411587007334
2211	15	0.013452526454059921
2211	16	0.06670618559240893
2211	17	-0.013304104113053938
2211	18	-0.14837796512148316
2211	19	0.11610909457324681
2211	20	-0.19066476099477825
2211	21	0.20537499685653415
2211	22	0.17524172254801584
2211	23	-0.43547854711576406
2211	24	0.08942421260598366
2211	25	-0.8226923567241755
2212	1	-0.10851376985818462
2212	2	-0.22804046173813575
2212	3	-0.10406427464469203
2212	4	-0.012001850635215305
2212	5	-0.03004322210482968
2212	6	-0.06239275290102597
2212	7	0.00843407457427503
2212	8	-0.17623001516471248
2212	9	0.07060436362478129
2212	10	-0.22210788670945902
2212	11	0.08068739142072445
2212	12	-0.17259128078102037
2212	13	-0.10462276630372003
2212	14	-0.09594341364715575
2212	15	0.10769099947267335
2212	16	-0.0971235737875844
2212	17	-0.4739945447697167
2212	18	-0.12481343759026231
2212	19	-0.2472258097285831
2212	20	-0.46695360973669153
2212	21	0.057131863298032215
2212	22	-0.14948270339391456
2212	23	-0.07461006336219356
2212	24	-0.32629725467860027
2212	25	0.10949547874305665
2213	1	-0.18779148159370607
2213	2	-0.09175171910147739
2213	3	-0.166134843191017
2213	4	0.058526516910959
2213	5	-0.021594056561617947
2213	6	-0.07176669447244421
2213	7	-0.1509099819549462
2213	8	-0.07888081840823702
2213	9	-0.25800292699729255
2213	10	-0.16716755717705922
2213	11	0.010289189836157675
2213	12	-0.029165990065969538
2213	13	0.11152511816735
2213	14	-0.6159484608987285
2213	15	-0.18560246500064892
2213	16	-0.29338395883974755
2213	17	-0.2544371090391593
2213	18	-0.0916171327097004
2213	19	-0.2785191015327797
2213	20	-0.15673325735883736
2213	21	0.12931353779014643
2213	22	-0.47434274177958713
2213	23	-0.5048344668235458
2213	24	-0.6581278131304202
2213	25	-0.21482231215462377
2214	1	-0.15313261425141533
2214	2	-0.17067588327147476
2214	3	0.05167079128381502
2214	4	-0.11883520051018337
2214	5	-0.2417896799757031
2214	6	-0.14578075432441295
2214	7	-0.29342879606932115
2214	8	-0.29062813764866746
2214	9	-0.07466468810373783
2214	10	-0.2876460506846783
2214	11	-0.2634801083795608
2214	12	-0.30817318729737764
2214	13	-0.5471643846567034
2214	14	-0.14169273765907064
2214	15	0.027817288812521213
2214	16	-0.21720980009168755
2214	17	-0.4233759279248446
2214	18	-0.46865353403203613
2214	19	-0.39173777901439877
2214	20	-0.46408323241793425
2214	21	-0.6658631088515037
2214	22	-0.6853681691055229
2214	23	-1.1574074073402727
2214	24	-0.44202435641449966
2214	25	-0.89226923141117
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	8.0
10	32.0
11	85.0
12	200.0
13	480.0
14	1060.0
15	2002.0
16	3170.0
17	4317.0
18	5105.0
19	5486.0
20	5434.0
21	5482.0
22	5590.0
23	5790.0
24	6256.0
25	6602.0
26	7591.0
27	8084.0
28	9563.0
29	11705.0
30	14970.0
31	19339.0
32	25757.0
33	34975.0
34	46863.0
35	65110.0
36	91332.0
37	142046.0
38	276296.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.670362839757196	19.72033030997732	12.460942659936027	23.148364190329456
2	17.118952848841726	21.508807461889425	36.003468466190476	25.368771223078372
3	19.452407389338397	26.576940985829733	29.073319063845872	24.897332560986
4	12.767143768218203	18.754864869199007	33.522973284698004	34.95501807788478
5	13.537143223717973	38.36948797915598	31.528808574473967	16.564560222652087
6	30.713726167492183	36.909222124215866	15.79758328655757	16.579468421734383
7	26.203997636111726	35.869290302999644	18.870762181242124	19.0559498796465
8	26.926592997706532	33.61675773505576	18.993341664990876	20.463307602246832
9	26.401449554230005	15.818297538520746	20.287715299260423	37.49253760798883
10	18.430832060964722	29.562292814429835	28.30205968925725	23.704815435348195
11	32.31928041695927	25.752147184747365	20.6678950986551	21.26067729963826
12	23.16900635360377	28.17714108799451	27.98052597626064	20.673326582141083
13	29.888298475945195	22.736119950859223	25.507689347404565	21.867892225791014
14	22.925557896398775	24.081191626250117	27.465573980787543	25.52767649656357
15	25.69607965275268	27.568738366807732	25.537701719091327	21.197480261348257
16	21.277931548279827	28.658422265310858	26.519244337216247	23.544401849193076
17	20.65488884749973	27.711219353350607	27.409147031143423	24.224744768006236
18	20.816450379843328	24.54605669712074	31.351043320271017	23.286449602764918
19	21.67336023919243	25.44367531403874	32.805083825894755	20.077880620874076
20	22.43158034960998	23.041399624043457	33.4015432966751	21.12547672967146
21	22.890632998202854	23.83891534655847	32.34434687793332	20.926104777305362
22	22.912660399724192	23.849741399195025	31.77366971379373	21.463928487287053
23	22.799794259748303	23.973796487367576	32.3437737054584	20.882635547425718
24	22.33633020982247	23.79167987487866	32.06172685705405	21.81026305824482
25	22.296436364354022	24.10606907219165	32.79023383005414	20.807260733400188
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1456.0
1	1456.0
2	1576.0
3	1696.0
4	1696.0
5	1696.0
6	3650.0
7	5604.0
8	5604.0
9	5604.0
10	5796.5
11	5989.0
12	5989.0
13	5989.0
14	5355.0
15	4721.0
16	4721.0
17	4721.0
18	8280.0
19	11839.0
20	11839.0
21	11839.0
22	18456.0
23	25073.0
24	25073.0
25	25073.0
26	34609.5
27	44146.0
28	44146.0
29	44146.0
30	51696.5
31	59247.0
32	59247.0
33	59247.0
34	63881.0
35	68515.0
36	68515.0
37	68515.0
38	64468.5
39	60422.0
40	60422.0
41	60422.0
42	67914.0
43	75406.0
44	75406.0
45	75406.0
46	88013.0
47	100620.0
48	100620.0
49	100620.0
50	98354.5
51	96089.0
52	96089.0
53	96089.0
54	89665.0
55	83241.0
56	83241.0
57	83241.0
58	73380.0
59	63519.0
60	63519.0
61	63519.0
62	57328.0
63	51137.0
64	51137.0
65	51137.0
66	38819.5
67	26502.0
68	26502.0
69	26502.0
70	20712.0
71	14922.0
72	14922.0
73	14922.0
74	10859.0
75	6796.0
76	6796.0
77	6796.0
78	4697.5
79	2599.0
80	2599.0
81	2599.0
82	1711.0
83	823.0
84	823.0
85	823.0
86	543.0
87	263.0
88	263.0
89	263.0
90	179.5
91	96.0
92	96.0
93	96.0
94	52.0
95	8.0
96	8.0
97	8.0
98	6.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08708169027287652
2	0.0
3	0.1332127839868366
4	0.009127542606505471
5	0.01739166902050367
6	0.0157881818058473
7	0.025162414753069136
8	0.020968678960890948
9	1.2334517035818203E-4
10	0.3927310224204516
11	0.059945752794076476
12	0.0014801420442981845
13	1.2334517035818203E-4
14	0.0011101065332236384
15	0.0017268323850145486
16	1.2334517035818203E-4
17	0.0
18	4.933806814327281E-4
19	6.167258517909102E-4
20	1.2334517035818203E-4
21	4.933806814327281E-4
22	0.002960284088596369
23	2.4669034071636407E-4
24	0.0
25	0.0019735227257309126
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	810733.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.71571225954323
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.70399504255938	38.82836888429383
2	12.096774506508082	11.786059722552515
3	3.686417942881294	5.387594273214671
4	1.5331854807650418	2.9876089088583693
5	0.7539360169331131	1.8364265031509823
6	0.4725878313878972	1.38134716867526
7	0.30930910637521636	1.0547749397802042
8	0.21772149465245	0.8485166148965149
9	0.16035971037248517	0.703083375767671
>10	0.8708016618248487	7.821209917954707
>50	0.09489105831622204	3.205394047959445
>100	0.08309366093693867	8.23611334726546
>500	0.010514938575292704	3.836573050985591
>1k	0.005385700245881629	4.766730871898309
>5k	2.564619164705538E-4	1.0336058633191285
>10k+	7.693857494116612E-4	6.286592509427281
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	21089	2.6012262976837013	No Hit
GGTATCAACGCAGAGTACTTTTTTT	15611	1.9255414544615799	No Hit
TATCAACGCAGAGTACTTTTTTTTT	13618	1.6797145299377232	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	8273	1.02043459437324	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4064	0.5012747723356519	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3491	0.4305979897204135	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2976	0.36707522698594974	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	2756	0.3399392895071497	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	2295	0.28307716597202776	No Hit
ATACAGGGTGACAGCCCCGTACACA	2256	0.2782667043280587	No Hit
GGATACCACGTGTCCCGCCCTACTC	1965	0.24237325975382773	No Hit
GGTATCAACGCAGAGTACATGGGGG	1960	0.24175653390203683	No Hit
GTTAATGATAGTGTGTCGAAACACA	1869	0.23053212339944223	No Hit
GTGGTATCAACGCAGAGTACTTTTT	1747	0.21548401261574401	No Hit
ACTTAGATGTTTCAGTTCCCCCGGT	1594	0.19661220155094217	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	1449	0.17872715184900578	No Hit
AGCGTACACGGTGGATGCCCTGGCA	1387	0.17107975128679848	No Hit
TCATTAACCTATGGATTCAGTTAAT	1087	0.13407620017934388	No Hit
CCCATATTCAGACAGGATACCACGT	1075	0.13259605813504569	No Hit
GTATCAACGCAGAGTACATGGGGGT	1057	0.13037584506859842	No Hit
GTACTTAGATGTTTCAGTTCCCCCG	1033	0.12741556098000206	No Hit
GTGGATGCCCTGGCAGTCAGAGGCG	1030	0.1270455254689275	No Hit
CTATCGGTCAGTCAGGAGTATTTAG	1027	0.12667548995785297	No Hit
GTTCACTATCGGTCAGTCAGGAGTA	1018	0.1255653834246293	No Hit
GCCTTGGAGGATGGTCCCCCCATAT	1017	0.12544203825427114	No Hit
CAACGCAGAGTACTTTTTTTTTTTT	991	0.12223506382495838	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	985	0.1214949928028093	No Hit
GTCAGGAGTATTTAGCCTTGGAGGA	972	0.11989150558815295	No Hit
GTACATGGGGGTTAAGCGACTAAGC	966	0.11915143456600384	No Hit
ACGCAGAGTACATGGGGGTTAAGCG	965	0.11902808939564567	No Hit
GCCCAGAGCCTGAATCAGTGTGTGT	955	0.11779463769206384	No Hit
GGTATCAACGCAGAGTACATGGGGA	939	0.11582111496633293	No Hit
GTGGTATCAACGCAGAGTACATGGG	930	0.11471100843310929	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG	910	0.11224410502594566	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	907	0.11187406951487111	No Hit
CCTATGGATTCAGTTAATGATAGTG	898	0.11076396298164747	No Hit
ATCATTAACTGAATCCATAGGTTAA	870	0.10731029821161837	No Hit
CGGTACTGGTTCACTATCGGTCAGT	869	0.10718695304126019	No Hit
GGGTTTCCCCATTCGGAAATCGCCG	868	0.107063607870902	No Hit
CACACACACTGATTCAGGCTCTGGG	850	0.10484339480445473	No Hit
AACGCAGAGTACTTTTTTTTTTTTT	848	0.10459670446373837	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	1.2334517035818203E-4	0.0
13	0.0	0.0	0.0	1.2334517035818203E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTTAA	50	8.648861E-5	15.211678	1
GGTATCA	2820	0.0	15.137507	1
TGTATAG	40	0.0051914947	14.284802	5
CTATACC	55	1.9182106E-4	13.85193	4
TAGAGAG	65	7.853715E-4	11.720864	5
GTGTAGG	65	7.9646404E-4	11.701291	1
TATATCC	65	8.018534E-4	11.69189	3
TCTAAGC	65	8.018534E-4	11.69189	3
TGTAAGG	100	1.9264553E-6	11.399593	2
ACAGGGT	485	0.0	11.164549	3
AGAGTAT	60	0.0057658628	11.110402	10
TCCTAAA	60	0.0058730976	11.082936	2
GTACATA	130	3.2187017E-8	10.96996	1
ATTCTAA	70	0.001480546	10.865484	1
GTGTAAG	150	2.0972948E-9	10.774938	1
TCACGGT	195	1.8189894E-12	10.726183	1
TAAGGCA	80	3.6824914E-4	10.713602	4
AGGTAAC	90	9.269059E-5	10.581336	6
ACCGGGG	220	0.0	10.342173	15
CCGGGGG	240	0.0	10.270353	16
>>END_MODULE