FastQCFastQC Report
Wed 25 May 2016
SRR1294679_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294679_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1952494
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC80510.4123444169354682No Hit
CCCATGTACTCTGCGTTGATACCAC67620.34632628832662227No Hit
GTATCAACGCAGAGTACTTTTTTTT66170.33889988906496No Hit
GGTATCAACGCAGAGTACTTTTTTT43760.22412360806230391No Hit
TATCAACGCAGAGTACTTTTTTTTT36990.18945000599233597No Hit
GAGTACATGGGAAGCAGTGGTATCA36390.18637701319440675No Hit
CATGTACTCTGCGTTGATACCACTG26420.13531411620214967No Hit
GTATCAACGCAGAGTACATGGGAAG23550.1206149673187216No Hit
ATTCCATTCCATTCCATTCCATTCC22190.11364951697674872No Hit
GGTATCAACGCAGAGTACATGGGAA22040.1128812687772664No Hit
GCGTTGATACCACTGCTTCCCATGT21340.10929611051301567No Hit
GTGGTATCAACGCAGAGTACATGGG21110.10811812994047612No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20620.1056085191555006No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTCG400.00528986514.2458869
CACACCG604.1019497E-412.6633355
TCAACGG701.0938592E-412.2110723
GACTGCG700.001495615210.853737
TCGATGA803.7566738E-410.69208813
ATTAGAC1553.6779966E-910.4166143
CTTACAC3200.010.3878913
GTCTTAG1951.4551915E-1110.2312251
GTATAGA4300.010.1632441
CCTAGAC1901.0004442E-109.9973693
TCTGGAC1901.0004442E-109.9973693
TAGACTG2400.09.893235
ACTATCG1451.6246668E-79.82449711
GTCTAGG1752.572051E-99.7718641
CTAGACA2350.09.6995754
GTCTAAT1804.1782187E-99.5004231
GTACTGG3800.09.5004231
TAGACAG3200.09.49755
CCTGTAC1901.0568328E-99.49753
TACACGG900.00111646469.49755
Produced by FastQC (version 0.11.5)