FastQCFastQC Report
Wed 25 May 2016
SRR1294679_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294679_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1952494
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT168910.8650986891637056No Hit
GGTATCAACGCAGAGTACTTTTTTT124560.6379533048501045No Hit
TATCAACGCAGAGTACTTTTTTTTT101650.5206161965158408No Hit
CCCATGTACTCTGCGTTGATACCAC79430.4068130298991956No Hit
GTACATGGGAAGCAGTGGTATCAAC78940.40430341911422013No Hit
ACGCAGAGTACTTTTTTTTTTTTTT64740.33157592289656207No Hit
GAGTACATGGGAAGCAGTGGTATCA40030.20501983616851063No Hit
CATGTACTCTGCGTTGATACCACTG29780.15252287587055324No Hit
GAGTACTTTTTTTTTTTTTTTTTTT26240.1343922183627709No Hit
GTATCAACGCAGAGTACATGGGAAG24790.12696581910110863No Hit
GGTATCAACGCAGAGTACATGGGAA24470.12532688960887972No Hit
ACGCAGAGTACATGGGAAGCAGTGG23880.12230511335758265No Hit
ATTCCATTCCATTCCATTCCATTCC23120.11841265581353899No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22910.11733710833426375No Hit
GCGTTGATACCACTGCTTCCCATGT22700.11626156085498855No Hit
GTGGTATCAACGCAGAGTACATGGG22600.115749395388667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAC852.763427E-713.38629316
CCGTACC550.0030250612.1141729
CCGTACA1052.7889837E-711.73956717
TATTCCG1001.88376E-611.4231085
TTCCGTA855.215531E-511.1991257
TAGCGGC600.005788644311.1058014
ACACCGT1053.3839679E-610.8791516
CCCCGTA1053.5458597E-610.83680215
TAGACTG2650.010.41735
CATAGAC2100.010.409263
TGTCGCC2151.8189894E-1210.182343510
CGCCCTA750.002690174210.11408916
AGCCCCG1054.1911353E-59.93373513
CTATACT3750.09.9000274
TCTGTCG2251.8189894E-129.7307968
GAACTGT2550.09.7059096
TAATACT3450.09.6572174
TGTGCAA2600.09.51827810
GTCCTAC1201.6714117E-59.5138761
GTCTTGC4000.09.5138761
Produced by FastQC (version 0.11.5)