##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294679_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1952494 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.221855739377432 31.0 31.0 34.0 28.0 34.0 2 31.39762017194419 31.0 31.0 34.0 28.0 34.0 3 31.46866264377765 31.0 31.0 34.0 28.0 34.0 4 35.02365743505486 37.0 35.0 37.0 32.0 37.0 5 35.17708633163534 37.0 35.0 37.0 32.0 37.0 6 34.72345523212876 37.0 35.0 37.0 32.0 37.0 7 34.56257535234423 37.0 35.0 37.0 31.0 37.0 8 34.601682770856144 37.0 35.0 37.0 31.0 37.0 9 36.0257250470424 39.0 35.0 39.0 30.0 39.0 10 35.806010671479655 39.0 35.0 39.0 30.0 39.0 11 35.95706875411653 39.0 35.0 39.0 30.0 39.0 12 35.90307627065691 39.0 35.0 39.0 30.0 39.0 13 35.746616891012216 39.0 35.0 39.0 30.0 39.0 14 36.79381549956108 40.0 36.0 41.0 30.0 41.0 15 36.844131659303436 40.0 36.0 41.0 30.0 41.0 16 36.88191359358851 39.0 36.0 41.0 30.0 41.0 17 36.948684605432845 40.0 36.0 41.0 30.0 41.0 18 36.97112974482892 40.0 36.0 41.0 30.0 41.0 19 36.993878086181056 40.0 36.0 41.0 30.0 41.0 20 37.07740049393237 40.0 36.0 41.0 30.0 41.0 21 36.9223946398811 40.0 36.0 41.0 30.0 41.0 22 36.90047242142614 40.0 36.0 41.0 30.0 41.0 23 36.91888989159506 40.0 36.0 41.0 30.0 41.0 24 36.8524917362102 40.0 36.0 41.0 29.0 41.0 25 35.76344357524274 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5332296268116608 1101 2 0.5243833439459387 1101 3 0.4817022284432717 1101 4 0.40927792986583 1101 5 0.45462748105292405 1101 6 0.5226852924258267 1101 7 0.5900358742697804 1101 8 0.5133752989906952 1101 9 0.7912319456075352 1101 10 0.8232693154599104 1101 11 0.7147867783870652 1101 12 0.7954305657497045 1101 13 0.8786414659845505 1101 14 1.0715270786707407 1101 15 0.9553603732626854 1101 16 1.0777209686217546 1101 17 1.1008054289840459 1101 18 0.9859135591803039 1101 19 1.192285822768902 1101 20 1.008968308091788 1101 21 1.2588766339884288 1101 22 1.1967757299454789 1101 23 1.1126143395514632 1101 24 1.1639079643406944 1101 25 1.2248323148908398 1102 1 0.5085786097833918 1102 2 0.46737379899936116 1102 3 0.40142762049229574 1102 4 0.3833026455281683 1102 5 0.37845614908808756 1102 6 0.47447483214900643 1102 7 0.5370355959399191 1102 8 0.600724500306768 1102 9 0.7303294068441986 1102 10 0.7800284079078068 1102 11 0.7071744603046284 1102 12 0.8036444402456411 1102 13 0.7957801580590456 1102 14 1.1007445397979367 1102 15 0.992304945974567 1102 16 0.9233964155860122 1102 17 0.841906214350594 1102 18 0.9020308701513855 1102 19 0.9798073400786436 1102 20 0.8901485273074314 1102 21 1.2699020617107166 1102 22 1.0747852964158398 1102 23 1.0885356628069331 1102 24 1.1828578419620968 1102 25 1.1643402072769575 1103 1 0.3000747043587211 1103 2 0.1595188797257805 1103 3 0.19371083140299206 1103 4 0.09096951829853595 1103 5 0.15482289971333785 1103 6 0.1360776381195734 1103 7 0.24181094845310724 1103 8 0.257954947559206 1103 9 0.0804238186216395 1103 10 0.28313221127969257 1103 11 0.29186609845164924 1103 12 0.36548520903836135 1103 13 0.4662460153338941 1103 14 0.6500500591219165 1103 15 0.6272292000882231 1103 16 0.6776754355980472 1103 17 0.5971546248174064 1103 18 0.6973782547989913 1103 19 0.7474274491396926 1103 20 0.5662142629181943 1103 21 0.7898823171979075 1103 22 0.7721462439452367 1103 23 0.7713145327739923 1103 24 0.8956576220194705 1103 25 0.8596872589024187 1104 1 0.12010322103734339 1104 2 0.14490380947848536 1104 3 0.0357411462670818 1104 4 0.07212199171173239 1104 5 0.11607472741447822 1104 6 0.08408501249633815 1104 7 0.24229772997357202 1104 8 0.17938732034667026 1104 9 -0.14780042782058445 1104 10 0.34656928841094725 1104 11 0.30503826108753174 1104 12 0.2408193718377163 1104 13 -0.11109902739571709 1104 14 0.22454888303597187 1104 15 0.3986424103941886 1104 16 0.3873465856193903 1104 17 0.4476124355720117 1104 18 0.46750585331322014 1104 19 0.6203458678262948 1104 20 0.4512949542206073 1104 21 0.6591654950820427 1104 22 0.6359927261313985 1104 23 0.555416756893294 1104 24 0.6437979531398881 1104 25 0.60763868973131 1105 1 -0.033165613987101494 1105 2 0.043206256426522316 1105 3 0.04185622983501247 1105 4 -0.024925975933022926 1105 5 -0.008956872366752577 1105 6 0.05983057526511715 1105 7 -0.088262139493267 1105 8 -0.09657149292834788 1105 9 0.19921657112395508 1105 10 0.2919822074624605 1105 11 0.09753772604058497 1105 12 -0.3119756219520795 1105 13 -0.5141676542548907 1105 14 -0.8475261296313832 1105 15 -0.1896997489272465 1105 16 0.020660346295180432 1105 17 0.1662575686569383 1105 18 -0.6316746925627115 1105 19 -0.5148615383209858 1105 20 -0.47962988128675477 1105 21 0.01982620946574798 1105 22 0.1297047812421468 1105 23 0.1890823132232171 1105 24 0.45810523654647994 1105 25 0.08829545299910535 1106 1 -0.4694852499058939 1106 2 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0.1875655128035163 2207 5 0.18317589519291033 2207 6 0.32644927908862087 2207 7 0.2799857123667522 2207 8 0.3568587551868063 2207 9 0.4230833488013914 2207 10 0.6018860648934634 2207 11 0.4066628779793291 2207 12 0.44106857500667473 2207 13 0.6104580331906604 2207 14 0.6198087843207674 2207 15 0.5753206539302482 2207 16 0.5921524037616308 2207 17 0.6258232479825026 2207 18 0.5622376180431132 2207 19 0.7095401245198474 2207 20 0.6283389406549205 2207 21 0.8421972288571311 2207 22 0.8023866957276553 2207 23 0.6348639245288581 2207 24 0.7490661884099907 2207 25 0.6544489503979634 2208 1 0.2194905463423069 2208 2 0.19334051569916966 2208 3 0.18224080204876358 2208 4 0.15056653186657343 2208 5 0.13742677627652 2208 6 0.22625891226945782 2208 7 0.17282735140124572 2208 8 0.19833106184718474 2208 9 0.2765532726111246 2208 10 0.2344015269152422 2208 11 -0.07998468114822543 2208 12 -0.2635446381870068 2208 13 -0.07978316384860307 2208 14 -0.40960856208410235 2208 15 0.1198453092889622 2208 16 0.2967598544725547 2208 17 0.21921337293105836 2208 18 0.25221028338602736 2208 19 0.4890507591177382 2208 20 0.3894366500808317 2208 21 0.6680194006882232 2208 22 0.6431568758782333 2208 23 0.6483428839115462 2208 24 0.6033077726880975 2208 25 0.5117280033633733 2209 1 -0.019461723927733487 2209 2 0.041142654385343036 2209 3 0.15735748447704268 2209 4 -0.48807619205898334 2209 5 -0.02743367849348033 2209 6 0.05239479927122659 2209 7 0.0540040259960648 2209 8 0.1819200024006875 2209 9 0.25116972680987715 2209 10 0.34231591776735115 2209 11 0.37592993439845657 2209 12 0.4082076928585465 2209 13 0.3863339737533025 2209 14 0.43729601946145635 2209 15 -0.2048970342883294 2209 16 -0.2049845492763538 2209 17 0.14069283760780138 2209 18 -0.09774451418382313 2209 19 0.3240456282916355 2209 20 0.3226836576773877 2209 21 0.5756606745110844 2209 22 0.5945615961868356 2209 23 0.5664494876054604 2209 24 0.5614268502731505 2209 25 0.6701858838636525 2210 1 -0.07931710681828363 2210 2 -0.09300185429217578 2210 3 -0.1063329868215277 2210 4 -0.039723334925319875 2210 5 0.016894786679259255 2210 6 -0.4574645112507554 2210 7 -0.13115547756915902 2210 8 -0.19757551898457848 2210 9 0.016008385253300617 2210 10 0.22281271440644446 2210 11 0.1456415315759898 2210 12 0.0989411520672121 2210 13 0.14600338504753552 2210 14 0.13737303671430112 2210 15 -0.057458719603438624 2210 16 0.12002003933117322 2210 17 -1.112197634735594 2210 18 -1.570956825502222 2210 19 -0.49911157195506917 2210 20 -0.24961492666153617 2210 21 -0.5119142300073349 2210 22 -0.38126566397074413 2210 23 -0.17708955868917542 2210 24 0.10590200435703423 2210 25 0.18657168825053105 2211 1 -0.2606454196622465 2211 2 -0.2696633526164547 2211 3 -0.09346352117341894 2211 4 -0.10643885170907907 2211 5 -0.07994555518037316 2211 6 0.04209515379375972 2211 7 -0.14412759359665017 2211 8 -0.19154363679282227 2211 9 -0.15122158839695032 2211 10 0.012525075146967879 2211 11 -0.2269033348140539 2211 12 -0.14008077740168545 2211 13 -0.06926800829132418 2211 14 -0.20273522165852853 2211 15 -0.24388366286402885 2211 16 -0.23607614589804626 2211 17 -0.41902828147532034 2211 18 -0.43197765616915973 2211 19 -0.12354640052534904 2211 20 -0.29994233642967316 2211 21 0.024576514360980184 2211 22 -0.03561949579021473 2211 23 -0.799321637779471 2211 24 -0.17141294570024002 2211 25 -0.7419784370174511 2212 1 -0.1879653214685426 2212 2 -0.272860176464242 2212 3 -0.24822395511294815 2212 4 -0.09409383317843378 2212 5 -0.12155240744993989 2212 6 -0.2208616524453646 2212 7 -0.22852775388767554 2212 8 -0.499294641788012 2212 9 -0.27461930483361385 2212 10 -0.6131455254258569 2212 11 -0.2534899429591633 2212 12 -0.3019646845331607 2212 13 -0.2576000030046046 2212 14 -0.3831622729320827 2212 15 -0.26988102637199773 2212 16 -0.5498018717488904 2212 17 -0.7358627878610378 2212 18 -0.4867012451497317 2212 19 -0.5405301066749573 2212 20 -0.8101685122834894 2212 21 -0.1818336843786028 2212 22 -0.47254885820073156 2212 23 -0.5059160613138189 2212 24 -0.5751135119039006 2212 25 -0.24744278823197874 2213 1 -0.12048785023952391 2213 2 -0.1393101485539674 2213 3 -0.13483519955456913 2213 4 0.062127372166521866 2213 5 0.06352821479872262 2213 6 -0.24156156241941318 2213 7 -0.216576126679783 2213 8 -0.2122406212046002 2213 9 -0.17403403867211864 2213 10 -0.2317640449223859 2213 11 0.1382746046437333 2213 12 0.11683995915683454 2213 13 0.1861458257745099 2213 14 -0.733592806993201 2213 15 -0.20441744008580542 2213 16 -0.21605761396715906 2213 17 -0.19916509678151328 2213 18 -0.10386687061281208 2213 19 -0.2177338761773342 2213 20 -0.20150918306594434 2213 21 0.07767015342316341 2213 22 -0.24935226013428746 2213 23 -0.28984285005032717 2213 24 -0.6043440908665687 2213 25 -0.33818940473814507 2214 1 -0.2721909263249991 2214 2 -0.08905664372206346 2214 3 -0.036449804155086696 2214 4 -0.034748903864510794 2214 5 -0.19511817148138988 2214 6 -0.27773789289443584 2214 7 -0.08323088372347343 2214 8 -0.07978349584731603 2214 9 -0.27838785655696796 2214 10 -0.18257049653528412 2214 11 0.037624679504219216 2214 12 0.04367063834561691 2214 13 -0.2390430268183863 2214 14 -0.06634597155016309 2214 15 -0.13896385358791719 2214 16 -0.31073741524659226 2214 17 -0.14519408820658697 2214 18 -0.313391166243683 2214 19 -0.393159219855022 2214 20 -0.4615353842364911 2214 21 -0.5601446008983331 2214 22 -0.5825069491229868 2214 23 -0.9053474777895403 2214 24 -0.3124018014967618 2214 25 -0.6164509400586837 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 4.0 9 30.0 10 56.0 11 154.0 12 387.0 13 986.0 14 2093.0 15 3611.0 16 5285.0 17 6895.0 18 8242.0 19 9377.0 20 9942.0 21 10574.0 22 11373.0 23 12010.0 24 13450.0 25 15072.0 26 16778.0 27 18676.0 28 22140.0 29 27240.0 30 34417.0 31 44799.0 32 59246.0 33 79869.0 34 108813.0 35 150644.0 36 213385.0 37 337866.0 38 729080.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.09449109532159 20.994432653563408 11.965488603857157 23.945587647257852 2 14.939559353319396 21.664496792307684 39.81615308420922 23.579790770163697 3 18.59421374306541 27.50597873554936 29.094170918753303 24.805636602631925 4 11.62348392569379 17.692135110021994 37.85875345627549 32.82562750800872 5 12.475666282791604 39.37395236943808 33.901427441465884 14.248953906304433 6 30.471404357894617 38.46290964429667 17.165529615991208 13.900156381817503 7 25.835551892251036 34.31475856939193 22.079275936136238 17.770413602220795 8 25.047972649698742 36.78347097616274 20.372470614510945 17.79608575962757 9 25.18115311099866 16.344189886565477 22.14257471989642 36.33208228253944 10 15.234687927734855 30.638541616319714 32.50404403862944 21.62272641731599 11 31.862239890695538 26.33464651066728 21.78750306855052 20.015610530086658 12 21.214758119326742 28.911210461000447 30.947553126269224 18.92647829340359 13 27.088226647559484 23.944964747650953 26.66671446877686 22.30009413601271 14 20.656743906854917 23.413492884181718 28.771035415965635 27.158727792997727 15 23.72322739187754 29.601139484404822 24.96545387310995 21.710179250607695 16 20.928730602363235 31.471406043453165 26.413923122899803 21.1859402312838 17 18.93081361581649 30.351079183854086 29.166645326438907 21.55146187389052 18 19.206317256963278 29.411168183445476 30.934861226134995 20.447653333456252 19 19.84778333196755 28.313836761452105 31.98286282061788 19.855517085962468 20 21.978425519797266 27.468896159369667 30.814005896054887 19.73867242477818 21 22.314161755419104 28.256570708904675 29.564489669862752 19.864777865813465 22 20.429688420323718 27.98926265520339 31.117915139942987 20.463133784529905 23 20.085039997582577 28.427400251883107 30.88021314288963 20.60734660764469 24 20.067667301410403 27.960188353971894 31.061452685642056 20.91069165897565 25 20.675671038418734 27.813593012708587 30.014663560156706 21.496072388715973 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1563.0 1 1563.0 2 1651.5 3 1740.0 4 1740.0 5 1740.0 6 3542.0 7 5344.0 8 5344.0 9 5344.0 10 7690.5 11 10037.0 12 10037.0 13 10037.0 14 14158.5 15 18280.0 16 18280.0 17 18280.0 18 28773.0 19 39266.0 20 39266.0 21 39266.0 22 57548.0 23 75830.0 24 75830.0 25 75830.0 26 98766.5 27 121703.0 28 121703.0 29 121703.0 30 145003.0 31 168303.0 32 168303.0 33 168303.0 34 184686.0 35 201069.0 36 201069.0 37 201069.0 38 209401.0 39 217733.0 40 217733.0 41 217733.0 42 218182.0 43 218631.0 44 218631.0 45 218631.0 46 230722.0 47 242813.0 48 242813.0 49 242813.0 50 227789.5 51 212766.0 52 212766.0 53 212766.0 54 188170.5 55 163575.0 56 163575.0 57 163575.0 58 140881.0 59 118187.0 60 118187.0 61 118187.0 62 96593.0 63 74999.0 64 74999.0 65 74999.0 66 56237.5 67 37476.0 68 37476.0 69 37476.0 70 26449.0 71 15422.0 72 15422.0 73 15422.0 74 10478.0 75 5534.0 76 5534.0 77 5534.0 78 3597.5 79 1661.0 80 1661.0 81 1661.0 82 1049.0 83 437.0 84 437.0 85 437.0 86 269.0 87 101.0 88 101.0 89 101.0 90 60.5 91 20.0 92 20.0 93 20.0 94 11.5 95 3.0 96 3.0 97 3.0 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09393114652336959 2 0.0 3 0.1365945298679535 4 0.012701703564774078 5 0.024071776917112164 6 0.011318856805705933 7 0.020537835199493572 8 0.017413625854932203 9 1.0243309326430708E-4 10 0.39211388101576755 11 0.06386703365029546 12 0.0011779805725395315 13 0.0 14 6.65815106217996E-4 15 0.0016389294922289134 16 5.121654663215354E-5 17 0.0 18 5.121654663215354E-5 19 7.170316528501496E-4 20 1.0243309326430708E-4 21 1.5364963989646064E-4 22 0.0028681266114005985 23 5.121654663215354E-5 24 0.0 25 0.001792579132125374 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1952494.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.96477304804297 #Duplication Level Percentage of deduplicated Percentage of total 1 91.9587648200464 72.61502993793323 2 6.222731600751154 9.827531771844 3 0.9066555876418134 2.147815581326275 4 0.2884603528583382 0.9111282518726828 5 0.152718057552066 0.6029673377468429 6 0.08148458497418268 0.38606470556401845 7 0.054625444991923525 0.30194401065049176 8 0.04999092174020693 0.31580174317423343 9 0.03562099025582086 0.25315230701677005 >10 0.21217347911009482 3.1860865813004264 >50 0.019266809100095168 1.058590507710767 >100 0.013929196569382855 2.0540718562011966 >500 0.001821605880705946 0.9847573641259368 >1k 0.0013662044105294505 2.1790627113636427 >5k 1.9517205864706436E-4 1.1461694168195353 >10k+ 1.9517205864706436E-4 2.029825915349983 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 16891 0.8650986891637056 No Hit GGTATCAACGCAGAGTACTTTTTTT 12456 0.6379533048501045 No Hit TATCAACGCAGAGTACTTTTTTTTT 10165 0.5206161965158408 No Hit CCCATGTACTCTGCGTTGATACCAC 7943 0.4068130298991956 No Hit GTACATGGGAAGCAGTGGTATCAAC 7894 0.40430341911422013 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 6474 0.33157592289656207 No Hit GAGTACATGGGAAGCAGTGGTATCA 4003 0.20501983616851063 No Hit CATGTACTCTGCGTTGATACCACTG 2978 0.15252287587055324 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2624 0.1343922183627709 No Hit GTATCAACGCAGAGTACATGGGAAG 2479 0.12696581910110863 No Hit GGTATCAACGCAGAGTACATGGGAA 2447 0.12532688960887972 No Hit ACGCAGAGTACATGGGAAGCAGTGG 2388 0.12230511335758265 No Hit ATTCCATTCCATTCCATTCCATTCC 2312 0.11841265581353899 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2291 0.11733710833426375 No Hit GCGTTGATACCACTGCTTCCCATGT 2270 0.11626156085498855 No Hit GTGGTATCAACGCAGAGTACATGGG 2260 0.115749395388667 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCGTAC 85 2.763427E-7 13.386293 16 CCGTACC 55 0.00302506 12.114172 9 CCGTACA 105 2.7889837E-7 11.739567 17 TATTCCG 100 1.88376E-6 11.423108 5 TTCCGTA 85 5.215531E-5 11.199125 7 TAGCGGC 60 0.0057886443 11.105801 4 ACACCGT 105 3.3839679E-6 10.879151 6 CCCCGTA 105 3.5458597E-6 10.836802 15 TAGACTG 265 0.0 10.4173 5 CATAGAC 210 0.0 10.40926 3 TGTCGCC 215 1.8189894E-12 10.1823435 10 CGCCCTA 75 0.0026901742 10.114089 16 AGCCCCG 105 4.1911353E-5 9.933735 13 CTATACT 375 0.0 9.900027 4 TCTGTCG 225 1.8189894E-12 9.730796 8 GAACTGT 255 0.0 9.705909 6 TAATACT 345 0.0 9.657217 4 TGTGCAA 260 0.0 9.518278 10 GTCCTAC 120 1.6714117E-5 9.513876 1 GTCTTGC 400 0.0 9.513876 1 >>END_MODULE