FastQCFastQC Report
Wed 25 May 2016
SRR1294678_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294678_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3334153
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC681502.0439973810440013No Hit
CCCATGTACTCTGCGTTGATACCAC638781.91586888784048No Hit
GAGTACATGGGAAGCAGTGGTATCA315120.9451275931248506No Hit
CATGTACTCTGCGTTGATACCACTG249410.7480460554749587No Hit
GCGTTGATACCACTGCTTCCCATGT203250.6096000993355734No Hit
GTATCAACGCAGAGTACATGGGAAG180800.542266656629135No Hit
GTATCAACGCAGAGTACTTTTTTTT176690.5299396878307624No Hit
ACGCAGAGTACATGGGAAGCAGTGG162360.48696025647293334No Hit
TATCAACGCAGAGTACATGGGAAGC143530.430484143949003No Hit
ACTCTGCGTTGATACCACTGCTTCC139310.4178272562776813No Hit
GGTATCAACGCAGAGTACATGGGAA124320.3728683116821574No Hit
GCTTCCCATGTACTCTGCGTTGATA113000.3389166603932093No Hit
GGTATCAACGCAGAGTACTTTTTTT109440.3282392859595825No Hit
GTACTGGTTCACTATCGGTCAGTCA102640.30784430108636285No Hit
TATCAACGCAGAGTACTTTTTTTTT100100.30022617438371907No Hit
GTGGTATCAACGCAGAGTACATGGG89410.26816405845802516No Hit
CAGTGGTATCAACGCAGAGTACATG83380.25007850569544954No Hit
ATACCACTGCTTCCCATGTACTCTG71580.21468720841545064No Hit
GGATACCACGTGTCCCGCCCTACTC69620.20880865395199322No Hit
GCAGTGGTATCAACGCAGAGTACAT68660.20592936196989162No Hit
GTTAATGATAGTGTGTCGAAACACA66810.20038072637938328No Hit
GTACTCTGCGTTGATACCACTGCTT66450.1993009918860952No Hit
CATGGGAAGCAGTGGTATCAACGCA65260.19573186953328178No Hit
GGGAAGCAGTGGTATCAACGCAGAG62480.18739391983511255No Hit
ACGCAGAGTACTTTTTTTTTTTTTT56610.1697882490695538No Hit
ACATGGGAAGCAGTGGTATCAACGC55680.16699893496189286No Hit
TACCACTGCTTCCCATGTACTCTGC55200.16555928897084207No Hit
CTGCTTCCCATGTACTCTGCGTTGA55100.1652593627227065No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA54040.1620801444924693No Hit
GTACATGGTAAGCAGTGGTATCAAC53840.16148029199619815No Hit
GCAGAGTACATGGGAAGCAGTGGTA53820.161420306746571No Hit
AGCGTACACGGTGGATGCCCTGGCA50840.15248250455213064No Hit
AAGCAGTGGTATCAACGCAGAGTAC50540.1515827258077239No Hit
CCATGTACTCTGCGTTGATACCACT50290.15083291018738493No Hit
GTACATGGGGGTTAAGCGACTAAGC50000.14996312406779172No Hit
GTTGATACCACTGCTTCCCATGTAC49750.14921330844745279No Hit
ATACAGGGTGACAGCCCCGTACACA49280.14780365508121554No Hit
GATACCACTGCTTCCCATGTACTCT47040.14108530712297845No Hit
ACTTAGATGTTTCAGTTCCCCCGGT46420.13922576438453782No Hit
CTTCCCATGTACTCTGCGTTGATAC45410.13619650927836846No Hit
CCACTGCTTCCCATGTACTCTGCGT45080.13520675265952103No Hit
GTGGATGCCCTGGCAGTCAGAGGCG44000.13196754917965672No Hit
GTACTTTTTTTTTTTTTTTTTTTTT43260.1297480949434534No Hit
ATGGGAAGCAGTGGTATCAACGCAG42480.12740867020799587No Hit
GAGTACTTTTTTTTTTTTTTTTTTT39600.11877079426169104No Hit
ACCATGTACTCTGCGTTGATACCAC39170.11748111139470804No Hit
ACCCTGTATCGCGCGCCTTTCCAGA38950.11682127364880976No Hit
GTATCAACGCAGAGTACATGGGGGT38310.11490174566074203No Hit
TCATTAACCTATGGATTCAGTTAAT37570.1126822914245387No Hit
GGTATCAACGCAGAGTACATGGGGG37540.11259231355009802No Hit
GTTCACTATCGGTCAGTCAGGAGTA37360.11205244630345398No Hit
TTCCCATGTACTCTGCGTTGATACC37110.11130263068311501No Hit
GTATTTAGCCTTGGAGGATGGTCCC36440.1092931248206066No Hit
CTATCGGTCAGTCAGGAGTATTTAG34210.10260476948718311No Hit
ATCATTAACTGAATCCATAGGTTAA33680.1010151603720645No Hit
GTACTTAGATGTTTCAGTTCCCCCG33500.10047529312542046No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG655.459052E-513.1530981
GGACCGT655.4698256E-513.1503346
ATCGCCG2700.013.01660619
AATCGCC2750.012.78934618
TGTCCCG14150.011.81185811
TAGACAG2550.011.1734865
AAATCGC3150.011.1632917
CCCGCCC15100.011.14031214
GTACTGG23400.011.0827031
TACTGGT21800.011.0653532
GAAATCG3350.011.06422116
CGCCCTA15200.010.9419716
CCGCCCT15500.010.79150415
CCGTACA12850.010.72422517
ACTGGTT22150.010.7190023
CCCGTAC12950.010.64141316
TGGTTCG1252.2509812E-710.6371595
GTACAAA8250.010.5933361
GTCCCGC15800.010.52613512
CTGGTTC24200.010.518014
Produced by FastQC (version 0.11.5)