FastQCFastQC Report
Wed 25 May 2016
SRR1294678_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294678_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3334153
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC731732.1946503354825047No Hit
GTACATGGGAAGCAGTGGTATCAAC661821.9849718954109183No Hit
GTATCAACGCAGAGTACTTTTTTTT500941.5024505474103917No Hit
GGTATCAACGCAGAGTACTTTTTTT342391.0269174809914243No Hit
GAGTACATGGGAAGCAGTGGTATCA328090.9840280275080358No Hit
TATCAACGCAGAGTACTTTTTTTTT304040.911895764831428No Hit
CATGTACTCTGCGTTGATACCACTG287160.8612682141461415No Hit
GCGTTGATACCACTGCTTCCCATGT218910.6565685497936058No Hit
ACGCAGAGTACTTTTTTTTTTTTTT204570.6135591258109631No Hit
ACGCAGAGTACATGGGAAGCAGTGG201920.60561108023537No Hit
GTATCAACGCAGAGTACATGGGAAG184880.5545036475530667No Hit
ACTCTGCGTTGATACCACTGCTTCC155440.46620536010195096No Hit
TATCAACGCAGAGTACATGGGAAGC151350.45393837655320557No Hit
GGTATCAACGCAGAGTACATGGGAA132970.39881193214588534No Hit
GCTTCCCATGTACTCTGCGTTGATA120010.3599414903875137No Hit
GTACTTTTTTTTTTTTTTTTTTTTT105120.31528247204012533No Hit
CAGTGGTATCAACGCAGAGTACATG101800.30532492060202393No Hit
GAGTACTTTTTTTTTTTTTTTTTTT100380.30106596787849865No Hit
GTACTGGTTCACTATCGGTCAGTCA97620.2927880034299566No Hit
GTGGTATCAACGCAGAGTACATGGG92730.27812160989612655No Hit
CATGGGAAGCAGTGGTATCAACGCA81110.24327017986277175No Hit
GCAGTGGTATCAACGCAGAGTACAT78160.23442235554277202No Hit
ATACCACTGCTTCCCATGTACTCTG76930.23073326269070438No Hit
GGATACCACGTGTCCCGCCCTACTC74290.22281520973992494No Hit
GGGAAGCAGTGGTATCAACGCAGAG71960.21582692815836588No Hit
ACATGGGAAGCAGTGGTATCAACGC71650.21489715678914556No Hit
GTTAATGATAGTGTGTCGAAACACA65590.1967216261521292No Hit
GTACTCTGCGTTGATACCACTGCTT64680.1939922972940954No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA62660.1879337870817566No Hit
CTGCTTCCCATGTACTCTGCGTTGA62470.18736392721029899No Hit
GCAGAGTACATGGGAAGCAGTGGTA61180.18349487860934996No Hit
CCATGTACTCTGCGTTGATACCACT61110.18328493023565506No Hit
AAGCAGTGGTATCAACGCAGAGTAC59070.17716643477368915No Hit
TACCACTGCTTCCCATGTACTCTGC58450.17530689203524855No Hit
GCAGAGTACTTTTTTTTTTTTTTTT57120.17131787293504527No Hit
AGCGTACACGGTGGATGCCCTGGCA57080.17119790243579103No Hit
ATACAGGGTGACAGCCCCGTACACA54310.1628899453624354No Hit
GTACATGGTAAGCAGTGGTATCAAC53440.1602805870036558No Hit
GATACCACTGCTTCCCATGTACTCT52890.1586309926389101No Hit
CTTCCCATGTACTCTGCGTTGATAC52620.15782119176894402No Hit
CCACTGCTTCCCATGTACTCTGCGT52360.1570413835237915No Hit
ATCAACGCAGAGTACTTTTTTTTTT51280.1538021800439272No Hit
ATGGGAAGCAGTGGTATCAACGCAG50170.15047299868962222No Hit
ACTTAGATGTTTCAGTTCCCCCGGT49360.148043596079724No Hit
GTTGATACCACTGCTTCCCATGTAC48960.14684389108718165No Hit
ACCCTGTATCGCGCGCCTTTCCAGA47800.1433647466088089No Hit
GTACATGGGGGTTAAGCGACTAAGC44700.13406703291660582No Hit
GTGGATGCCCTGGCAGTCAGAGGCG42950.1288183235742331No Hit
GTGGTATCAACGCAGAGTACTTTTT42660.1279485374546399No Hit
ACCATGTACTCTGCGTTGATACCAC42510.12749864808243652No Hit
GGTATCAACGCAGAGTACATGGGGG41160.12344964373260615No Hit
TCATTAACCTATGGATTCAGTTAAT38930.11676128839918265No Hit
CTATCGGTCAGTCAGGAGTATTTAG38810.11640137690141993No Hit
GTATCAACGCAGAGTACATGGGGGT36870.11058280768758962No Hit
CCCATATTCAGACAGGATACCACGT36200.10857330182508121No Hit
ATCAACGCAGAGTACATGGGAAGCA34370.10308465148420004No Hit
TTCCCATGTACTCTGCGTTGATACC34310.10290469573531869No Hit
CCTATGGATTCAGTTAATGATAGTG33960.10185495386684414No Hit
GTTCACTATCGGTCAGTCAGGAGTA33760.10125510137057298No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAC400.00520587814.2807167
AGACCCG801.9535528E-613.0906565
GGACCGT957.1913746E-813.0280216
TCGCGTT550.003024011612.11514710
CCCGAAC1001.9745312E-611.37916916
ATCGCCT700.001515116210.83730417
TGGTTCG1158.5847205E-710.7622795
TGTCCCG14200.010.75870611
CCGTACA12650.010.64454617
CCCCGTA12400.010.62973615
CCCGTAC12500.010.62055916
AGGACCG1105.897269E-610.3859765
TAGACAG3500.010.3365175
CTTAACG1201.5613987E-610.27286117
TCGGTCG1201.5613987E-610.27286115
CTATGAC1951.4551915E-1110.231873
TAACGCC1303.9433507E-710.21207419
GTACTGG21800.010.2061661
TAGGACC1409.399446E-810.2005114
TAGACTG3300.010.0974755
Produced by FastQC (version 0.11.5)