FastQCFastQC Report
Wed 25 May 2016
SRR1294677_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294677_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488774
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC169613.470110930614149No Hit
CCCATGTACTCTGCGTTGATACCAC165033.3764070920302633No Hit
GAGTACATGGGAAGCAGTGGTATCA77581.587236636973325No Hit
CATGTACTCTGCGTTGATACCACTG66521.3609561883406236No Hit
GTATCAACGCAGAGTACTTTTTTTT52601.0761619889765004No Hit
GCGTTGATACCACTGCTTCCCATGT49761.0180574253131303No Hit
GTATCAACGCAGAGTACATGGGAAG41610.8513136950819806No Hit
ACGCAGAGTACATGGGAAGCAGTGG38840.7946412861567923No Hit
GTACTGGTTCACTATCGGTCAGTCA38150.7805243323090018No Hit
TATCAACGCAGAGTACATGGGAAGC37250.7621109142466662No Hit
GGTATCAACGCAGAGTACTTTTTTT35880.7340816000851109No Hit
ACTCTGCGTTGATACCACTGCTTCC33870.6929582997458948No Hit
TATCAACGCAGAGTACTTTTTTTTT31490.6442650386477186No Hit
GGTATCAACGCAGAGTACATGGGAA31360.6416053227053812No Hit
GTTAATGATAGTGTGTCGAAACACA29230.5980268999578537No Hit
GGATACCACGTGTCCCGCCCTACTC27320.5589495349588971No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA25080.5131205833370842No Hit
GCTTCCCATGTACTCTGCGTTGATA24540.5020725324996829No Hit
GTGGTATCAACGCAGAGTACATGGG20970.4290326408524185No Hit
CAGTGGTATCAACGCAGAGTACATG19300.39486552067008474No Hit
ATACAGGGTGACAGCCCCGTACACA18830.38524962457086503No Hit
GTACATGGGGGTTAAGCGACTAAGC18450.37747507027787897No Hit
ACTTAGATGTTTCAGTTCCCCCGGT18380.37604291553969726No Hit
ATACCACTGCTTCCCATGTACTCTG18090.3701097030529447No Hit
GTACTCTGCGTTGATACCACTGCTT17820.36458567763424404No Hit
GTATTTAGCCTTGGAGGATGGTCCC16600.33962526648307806No Hit
GCAGTGGTATCAACGCAGAGTACAT16370.3349196152004812No Hit
AGCGTACACGGTGGATGCCCTGGCA16250.33246449279216983No Hit
GGGAAGCAGTGGTATCAACGCAGAG15910.32550831263528746No Hit
GCAGAGTACATGGGAAGCAGTGGTA15610.31937050661450894No Hit
GTGGATGCCCTGGCAGTCAGAGGCG15590.31896131954645707No Hit
ATCATTAACTGAATCCATAGGTTAA15510.31732457127424946No Hit
CATGGGAAGCAGTGGTATCAACGCA15120.30934542344723737No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15040.30770867517502976No Hit
ACCCTGTATCGCGCGCCTTTCCAGA14860.30402599156256266No Hit
TCATTAACCTATGGATTCAGTTAAT14860.30402599156256266No Hit
GTTCACTATCGGTCAGTCAGGAGTA14770.3021846497563291No Hit
CCCATATTCAGACAGGATACCACGT14770.3021846497563291No Hit
CTGCTTCCCATGTACTCTGCGTTGA14460.2958422502015246No Hit
GTTGATACCACTGCTTCCCATGTAC14430.29522846959944676No Hit
CTATCGGTCAGTCAGGAGTATTTAG13840.28315745109191565No Hit
TACCACTGCTTCCCATGTACTCTGC13560.27742883213918906No Hit
AAGCAGTGGTATCAACGCAGAGTAC13490.2759966774010074No Hit
GTACATGGTAAGCAGTGGTATCAAC13480.27579208386698145No Hit
ACATGGGAAGCAGTGGTATCAACGC12760.261061349417113No Hit
GCCTTGGAGGATGGTCCCCCCATAT12760.261061349417113No Hit
GTACTTAGATGTTTCAGTTCCCCCG12710.26003838174698324No Hit
CCACTGCTTCCCATGTACTCTGCGT12470.25512813693036046No Hit
CCTATGGATTCAGTTAATGATAGTG12370.253082201590101No Hit
GATACCACTGCTTCCCATGTACTCT12270.25103626624984143No Hit
TATTCAGACAGGATACCACGTGTCC12100.24755817617140027No Hit
CCATGTACTCTGCGTTGATACCACT12090.2473535826373743No Hit
GTCAGGAGTATTTAGCCTTGGAGGA11950.24448927316101104No Hit
GCCCAGAGCCTGAATCAGTGTGTGT11880.24305711842282934No Hit
GGTTAATGAGGCGAACCGGGGGAAC10890.22280235855426025No Hit
GTATCAACGCAGAGTACATGGGGGT10800.2209610167480267No Hit
GGTATCAACGCAGAGTACATGGGGG10790.22075642321400074No Hit
ATGGGAAGCAGTGGTATCAACGCAG10630.21748292666958552No Hit
ACCATGTACTCTGCGTTGATACCAC10270.21011755944465133No Hit
CTTCCCATGTACTCTGCGTTGATAC10260.20991296591062533No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG10150.20766243703633994No Hit
GTACTTTTTTTTTTTTTTTTTTTTT9560.19559141852880882No Hit
GCCTGAATCAGTGTGTGTGTTAGTG9450.19334088965452337No Hit
GTGCTAATCTGCGATAAGCGTCGGT9290.19006739311010815No Hit
GGTTCACTATCGGTCAGTCAGGAGT9240.1890444254399784No Hit
GGGTACTTAGATGTTTCAGTTCCCC8950.18311121295322583No Hit
GAGTACATGGGGGTTAAGCGACTAA8710.17820096813660302No Hit
CACACACACTGATTCAGGCTCTGGG8550.1749274715921878No Hit
CGGTACTGGTTCACTATCGGTCAGT8450.17288153625192829No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA8320.17022182030959093No Hit
GAATCAGTGTGTGTGTTAGTGGAAG8290.1696080397075131No Hit
ATGATAGTGTGTCGAAACACACTGG8050.16469779489089026No Hit
TTCCCATGTACTCTGCGTTGATACC7860.16081051774439722No Hit
TAATGATAGTGTGTCGAAACACACT7620.1559002729277744No Hit
TCTAAGTACCCCGAGGAAAAGAAAT7590.15528649232569655No Hit
GCCCTACTCATCGAGCTCACAGCAT7500.15344515051946298No Hit
TGATACCACTGCTTCCCATGTACTC7480.15303596345141107No Hit
GGAGTATTTAGCCTTGGAGGATGGT7450.15242218284933323No Hit
GAGTACTTTTTTTTTTTTTTTTTTT7240.14812571863478827No Hit
ATCAACGCAGAGTACATGGGAAGCA7160.14648897036258066No Hit
ACGCAGAGTACATGGGGGTTAAGCG7100.14526140915842495No Hit
TCACGGTACTGGTTCACTATCGGTC7030.1438292544202433No Hit
CATCTAAGTACCCCGAGGAAAAGAA6980.14280628675011356No Hit
GTCCCGCCCTACTCATCGAGCTCAC6960.14239709968206163No Hit
GGGTTTCCCCATTCGGAAATCGCCG6830.13973738373972427No Hit
GAACAAAAAAAAAAAAAAAAAAAAA6560.13421335832102363No Hit
GCGTACACGGTGGATGCCCTGGCAG6450.13196282944673818No Hit
GATATGAACCGTTATAACCGGCGAT6340.1297123005724527No Hit
GTACATGGGTAAGCAGTGGTATCAA6310.12909851997037486No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG6070.12418827515375205No Hit
CATTAACCTATGGATTCAGTTAATG6010.12296071394959633No Hit
GGTAAGCAGTGGTATCAACGCAGAG5970.12214233981349254No Hit
GGTGATATGAACCGTTATAACCGGC5970.12214233981349254No Hit
TATCAACGCAGAGTACATGGGGGTT5930.12132396567738873No Hit
GTTATAACCGGCGATTTCCGAATGG5890.12050559154128493No Hit
GATTCAGGCTCTGGGCTGCTCCCCG5790.11845965620102543No Hit
AGCTCACAGCATGTGCATTTTTGTG5620.11498156612258426No Hit
CCCCATGTACTCTGCGTTGATACCA5540.11334481785037664No Hit
AAAAAGTACTCTGCGTTGATACCAC5540.11334481785037664No Hit
AGTCAGGAGTATTTAGCCTTGGAGG5520.11293563078232476No Hit
CAGTTAATGATAGTGTGTCGAAACA5490.1123218501802469No Hit
GCAGAGTACTTTTTTTTTTTTTTTT5350.1094575407038836No Hit
CACTATCATTAACTGAATCCATAGG5340.10925294716985766No Hit
CCCCAGTAGCGGCGAGCGAACGGGG5330.10904835363583168No Hit
GGAAGCAGTGGTATCAACGCAGAGT5180.10597945062544244No Hit
CCCTGGCAGTCAGAGGCGATGAAGG5170.10577485709141647No Hit
TCCATGTACTCTGCGTTGATACCAC5060.10352432821713103No Hit
GAGTACATGGTAAGCAGTGGTATCA5000.10229676701297531No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC4920.10066001874076771No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACA250.00603379418.99590319
GTACAAA1750.017.9122581
TACATAA1650.016.1160852
CCGAATG1450.015.73525218
CGAATGG1550.015.31927719
CTGCTTG508.649739E-515.20918613
TACATGA1350.014.7730782
TCTTATG400.00525114614.25861215
ATCTAAA400.005283379514.2454682
TACAAAA3100.013.7859372
GTACATA2000.013.7734391
ACATGAA1800.013.7178573
CATAAAA2650.013.6169154
TTCATCG655.4068125E-513.16179414
GGTTATA1102.730303E-912.9530781
ACATAAA3250.012.8574483
CCTTCAT751.466086E-512.67302112
TACAAGA604.0940696E-412.6626392
AACCGAC701.0807907E-412.22291918
CTTCATC701.0818259E-412.22166713
Produced by FastQC (version 0.11.5)