FastQCFastQC Report
Wed 25 May 2016
SRR1294677_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294677_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488774
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC185623.7976651785896958No Hit
GTACATGGGAAGCAGTGGTATCAAC164363.362699325250525No Hit
GTATCAACGCAGAGTACTTTTTTTT160013.2737011379492365No Hit
GGTATCAACGCAGAGTACTTTTTTT120342.46207858846829No Hit
TATCAACGCAGAGTACTTTTTTTTT103272.112837425885992No Hit
GAGTACATGGGAAGCAGTGGTATCA82711.6921931199286377No Hit
CATGTACTCTGCGTTGATACCACTG75541.5454995560320313No Hit
ACGCAGAGTACTTTTTTTTTTTTTT66671.364025091351013No Hit
GTACTTTTTTTTTTTTTTTTTTTTT54361.1121704509650676No Hit
GCGTTGATACCACTGCTTCCCATGT53001.0843457303375386No Hit
ACGCAGAGTACATGGGAAGCAGTGG48991.0023037231931322No Hit
GTATCAACGCAGAGTACATGGGAAG42840.8764786997671725No Hit
TATCAACGCAGAGTACATGGGAAGC40130.8210338520461399No Hit
GAGTACTTTTTTTTTTTTTTTTTTT39780.8138730783552317No Hit
ACTCTGCGTTGATACCACTGCTTCC37190.7608833530425104No Hit
GTACTGGTTCACTATCGGTCAGTCA35780.7320356647448514No Hit
GGTATCAACGCAGAGTACATGGGAA33230.679864313568234No Hit
GGATACCACGTGTCCCGCCCTACTC27360.559767909095001No Hit
GTTAATGATAGTGTGTCGAAACACA27080.5540392901422744No Hit
GCTTCCCATGTACTCTGCGTTGATA26230.5366488397500685No Hit
GCAGAGTACTTTTTTTTTTTTTTTT24530.5018679389656568No Hit
CAGTGGTATCAACGCAGAGTACATG23210.47486159247423143No Hit
GTGGTATCAACGCAGAGTACATGGG22000.45010577485709136No Hit
ATACAGGGTGACAGCCCCGTACACA20770.42494077017189946No Hit
GGGAAGCAGTGGTATCAACGCAGAG20180.4128697516643684No Hit
ACTTAGATGTTTCAGTTCCCCCGGT19890.40693653917761585No Hit
CATGGGAAGCAGTGGTATCAACGCA19640.40182170082696705No Hit
GCAGTGGTATCAACGCAGAGTACAT18740.3834082827646315No Hit
GCAGAGTACATGGGAAGCAGTGGTA18730.38320368923060555No Hit
ATACCACTGCTTCCCATGTACTCTG18300.37440616726748965No Hit
AGCGTACACGGTGGATGCCCTGGCA17830.36479027116827No Hit
GTACTCTGCGTTGATACCACTGCTT17660.3613121810898289No Hit
ACCCTGTATCGCGCGCCTTTCCAGA17600.36008461988567314No Hit
ATCAACGCAGAGTACTTTTTTTTTT16850.34474010483372686No Hit
CCCATATTCAGACAGGATACCACGT16450.3365563634726888No Hit
ACATGGGAAGCAGTGGTATCAACGC16320.3338966475303515No Hit
CTGCTTCCCATGTACTCTGCGTTGA16080.32898640271372864No Hit
GTGGATGCCCTGGCAGTCAGAGGCG15980.32694046737346916No Hit
AAGCAGTGGTATCAACGCAGAGTAC15610.31937050661450894No Hit
ATCATTAACTGAATCCATAGGTTAA15610.31937050661450894No Hit
GTACATGGGGGTTAAGCGACTAAGC15510.31732457127424946No Hit
CTATCGGTCAGTCAGGAGTATTTAG15470.31650619713814565No Hit
TACCACTGCTTCCCATGTACTCTGC15000.30689030103892595No Hit
TCATTAACCTATGGATTCAGTTAAT14690.30054790148412147No Hit
CCTATGGATTCAGTTAATGATAGTG14090.28827228944256444No Hit
ATGGGAAGCAGTGGTATCAACGCAG14040.28724932177243473No Hit
GTATTTAGCCTTGGAGGATGGTCCC13870.28377123169399354No Hit
GTTGATACCACTGCTTCCCATGTAC13800.2823390769558119No Hit
GATACCACTGCTTCCCATGTACTCT13760.2815207028197081No Hit
CCATGTACTCTGCGTTGATACCACT13690.28008854808152644No Hit
CCACTGCTTCCCATGTACTCTGCGT13340.27292777439061816No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA13190.2698588713802289No Hit
GTCAGGAGTATTTAGCCTTGGAGGA13060.2671991554378915No Hit
GTACATGGTAAGCAGTGGTATCAAC12960.26515322009763204No Hit
GTTCACTATCGGTCAGTCAGGAGTA12850.2629026912233466No Hit
GCCTTGGAGGATGGTCCCCCCATAT12750.2608567558830871No Hit
GCCCAGAGCCTGAATCAGTGTGTGT12740.26065216234906113No Hit
GTGGTATCAACGCAGAGTACTTTTT12150.24858114384153002No Hit
GTACTTAGATGTTTCAGTTCCCCCG12130.24817195677347814No Hit
CTTCCCATGTACTCTGCGTTGATAC12130.24817195677347814No Hit
GGTATCAACGCAGAGTACATGGGGG11970.24489846022906292No Hit
TATTCAGACAGGATACCACGTGTCC11460.23446418999373944No Hit
GGTTAATGAGGCGAACCGGGGGAAC11280.23078150638127232No Hit
GCCTGAATCAGTGTGTGTGTTAGTG11170.22853097750698687No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG11060.22628044863270141No Hit
ACCATGTACTCTGCGTTGATACCAC10950.22402991975841594No Hit
GTATCAACGCAGAGTACATGGGGGT10880.2225977650202343No Hit
CGGTACTGGTTCACTATCGGTCAGT10100.20663946936621014No Hit
CACACACACTGATTCAGGCTCTGGG9770.19988788274335378No Hit
GGGTACTTAGATGTTTCAGTTCCCC9590.1962051991308867No Hit
ACGCAGAGTACATGGGGGTTAAGCG8870.18147446468101824No Hit
GAATCAGTGTGTGTGTTAGTGGAAG8780.17963312287478467No Hit
GTGCTAATCTGCGATAAGCGTCGGT8680.17758718753452515No Hit
ATGATAGTGTGTCGAAACACACTGG8530.1745182845241359No Hit
CATCTAAGTACCCCGAGGAAAAGAA7980.1632656401527086No Hit
GAGTACATGGGGGTTAAGCGACTAA7890.16142429834647504No Hit
ATCAACGCAGAGTACATGGGAAGCA7600.1554910858597225No Hit
TCACGGTACTGGTTCACTATCGGTC7600.1554910858597225No Hit
TCTAAGTACCCCGAGGAAAAGAAAT7590.15528649232569655No Hit
GGTTCACTATCGGTCAGTCAGGAGT7480.15303596345141107No Hit
TAATGATAGTGTGTCGAAACACACT7320.14976246690699585No Hit
GCGTACACGGTGGATGCCCTGGCAG7250.14833031216881423No Hit
GTACATGGGTAAGCAGTGGTATCAA7170.14669356389660662No Hit
CAGTTAATGATAGTGTGTCGAAACA7160.14648897036258066No Hit
GGAGTATTTAGCCTTGGAGGATGGT7140.14607978329452875No Hit
GCCCTACTCATCGAGCTCACAGCAT7100.14526140915842495No Hit
TGATACCACTGCTTCCCATGTACTC7070.1446476285563471No Hit
TTCCCATGTACTCTGCGTTGATACC7030.1438292544202433No Hit
CATTAACCTATGGATTCAGTTAATG6970.1426016932160876No Hit
GGTAAGCAGTGGTATCAACGCAGAG6880.14076035140985405No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG6670.13646388719530908No Hit
GGGTTTCCCCATTCGGAAATCGCCG6570.13441795185504957No Hit
GTCCCGCCCTACTCATCGAGCTCAC6480.13257661004881602No Hit
GATATGAACCGTTATAACCGGCGAT6450.13196282944673818No Hit
CAACGCAGAGTACTTTTTTTTTTTT6420.1313490488446603No Hit
GTTATAACCGGCGATTTCCGAATGG6350.12991689410647864No Hit
CCCCATGTACTCTGCGTTGATACCA6290.12868933290232296No Hit
GGTGATATGAACCGTTATAACCGGC6240.1276663652321932No Hit
AGCTCACAGCATGTGCATTTTTGTG6190.12664339756206344No Hit
TATCAACGCAGAGTACATGGGGGTT6050.12377908808570014No Hit
GGAAGCAGTGGTATCAACGCAGAGT5970.12214233981349254No Hit
CCCCAGTAGCGGCGAGCGAACGGGG5900.12071018507531087No Hit
CACTATCATTAACTGAATCCATAGG5730.11723209499686972No Hit
AGTCAGGAGTATTTAGCCTTGGAGG5620.11498156612258426No Hit
CCCTGGCAGTCAGAGGCGATGAAGG5470.11191266311219501No Hit
GAGTACATGGTAAGCAGTGGTATCA5440.11129888251011714No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC5350.1094575407038836No Hit
AACGCAGAGTACTTTTTTTTTTTTT5350.1094575407038836No Hit
GGGTGACAGCCCCGTACACAAAAAT5300.10843457303375385No Hit
CACTGATTCAGGCTCTGGGCTGCTC5290.10822997949972789No Hit
GATTCAGGCTCTGGGCTGCTCCCCG5270.107820792431676No Hit
CCGTTATAACCGGCGATTTCCGAAT5050.10331973468310507No Hit
TCGGTAAGGTGATATGAACCGTTAT4950.10127379934284557No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA4940.10106920580881962No Hit
AAAAAGTACTCTGCGTTGATACCAC4910.10045542520674176No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACAG250.00595506719.0391335
GTACAAA502.1376582E-719.0235021
GAAATCG1050.016.25581216
AATCGCC1001.8189894E-1216.12034418
GAGCCCC551.1044842E-515.5758739
ATCGCCG1055.456968E-1215.35271119
CGCAGAA456.739805E-414.7808892
TACAAAA1250.014.4430392
GGGAGCC602.5119085E-514.279357
CAAGCAG400.005204280414.27788459
AGAACAA1203.6379788E-1214.2529982
ACAACCC400.005297197514.23986111
AAATCGC1152.5465852E-1114.01769117
GCAGAAA551.9523277E-413.8210893
GACCGAT604.1469064E-412.64340818
TGACCGA604.1469064E-412.64340817
CAGGGTA550.003016400812.1158114
CTGACCG550.003105727712.068706516
ACTCCTG1103.893365E-812.06747215
AAAAGTA907.4137424E-611.6135552
Produced by FastQC (version 0.11.5)