##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294677_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 488774 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.904986762798348 31.0 31.0 34.0 28.0 34.0 2 30.97321666046066 31.0 31.0 34.0 27.0 34.0 3 31.062941154807742 31.0 31.0 34.0 27.0 34.0 4 34.47516643683993 37.0 35.0 37.0 31.0 37.0 5 34.95100189453613 37.0 35.0 37.0 32.0 37.0 6 34.29290633298825 37.0 35.0 37.0 30.0 37.0 7 34.219197420484726 37.0 35.0 37.0 30.0 37.0 8 34.2185713642706 37.0 35.0 37.0 30.0 37.0 9 35.47136508897773 39.0 35.0 39.0 28.0 39.0 10 35.33636199961536 39.0 35.0 39.0 28.0 39.0 11 35.4862533604488 39.0 35.0 39.0 28.0 39.0 12 35.51421515874412 39.0 35.0 39.0 29.0 39.0 13 35.229062920695455 39.0 35.0 39.0 27.0 39.0 14 36.0829258512114 39.0 36.0 41.0 27.0 41.0 15 36.05159644334601 39.0 36.0 41.0 27.0 41.0 16 36.059270746807314 39.0 36.0 41.0 27.0 41.0 17 36.36566388555856 39.0 36.0 41.0 28.0 41.0 18 36.40468601030333 39.0 36.0 41.0 28.0 41.0 19 36.36225944915237 39.0 36.0 41.0 27.0 41.0 20 36.57229721711875 39.0 36.0 41.0 28.0 41.0 21 36.396039887555396 39.0 36.0 41.0 27.0 41.0 22 36.51052019951961 39.0 36.0 41.0 28.0 41.0 23 36.51995605330889 39.0 35.0 41.0 28.0 41.0 24 36.39852365305847 39.0 35.0 41.0 28.0 41.0 25 35.27208075715975 38.0 33.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.38466016195379993 1101 2 0.35093547724454766 1101 3 0.36067212048379105 1101 4 0.365246854329655 1101 5 0.33950086674641966 1101 6 0.3017384440528943 1101 7 0.5327930743496054 1101 8 0.4110133649879515 1101 9 0.49088959359697526 1101 10 0.8867483743458422 1101 11 0.7194511641478201 1101 12 0.8417689656913865 1101 13 0.6216902539944087 1101 14 0.9712597045310005 1101 15 0.8243910691041805 1101 16 0.9877281685709747 1101 17 0.8118177161417393 1101 18 0.8015399938920709 1101 19 0.9303451409600925 1101 20 0.6889188807962583 1101 21 1.048664953045396 1101 22 0.8803897468160997 1101 23 1.050019402291376 1101 24 0.9662717275791408 1101 25 0.7903184109649217 1102 1 0.45619189342541944 1102 2 0.44142523651919063 1102 3 0.32533562357580337 1102 4 0.26802325354229595 1102 5 0.159695699822322 1102 6 0.057262524952676586 1102 7 0.5218514183242249 1102 8 0.4299881214466055 1102 9 0.5586439946274666 1102 10 0.593011025391661 1102 11 0.4651254126129629 1102 12 0.551484411259132 1102 13 0.6699490797543106 1102 14 0.7729459530184499 1102 15 0.8689874345249322 1102 16 0.6854837406073528 1102 17 0.6500583076670026 1102 18 0.5405562374166877 1102 19 0.756768610148697 1102 20 0.6389247358257322 1102 21 0.8082730564061791 1102 22 0.6762265420517792 1102 23 0.6551127203086935 1102 24 0.9083638132521656 1102 25 0.7721354775393934 1103 1 0.4011905040718382 1103 2 0.4326660710386747 1103 3 0.34968617166161664 1103 4 0.24795871948612103 1103 5 0.2637724601900189 1103 6 0.3182920076027642 1103 7 0.2777697810419113 1103 8 0.2736796055597779 1103 9 0.09559115949553387 1103 10 0.6742037145564055 1103 11 0.6181471148830013 1103 12 0.5413687830161251 1103 13 0.4086269822273607 1103 14 0.7369554573311206 1103 15 0.7517135163777127 1103 16 0.8042014523136487 1103 17 0.7876077147816218 1103 18 0.8336928307217093 1103 19 0.8781754298899926 1103 20 0.7361125707591825 1103 21 0.7458961843231151 1103 22 0.9935798518322585 1103 23 0.9459276754240662 1103 24 1.084939878943068 1103 25 1.158645704883007 1104 1 0.27703905593253353 1104 2 0.16565620297417283 1104 3 0.21014541491258854 1104 4 0.043195542977272794 1104 5 0.20342875967141794 1104 6 0.12682406735869733 1104 7 0.2702226280975282 1104 8 0.03436311792474811 1104 9 -0.0653317073498485 1104 10 0.45811980863098256 1104 11 0.47094634430013116 1104 12 0.5315230672043114 1104 13 -0.12983679110327984 1104 14 0.31652517798365665 1104 15 0.5346552075255033 1104 16 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2207 4 0.30907131686820577 2207 5 0.12873167010985753 2207 6 0.14589496051877404 2207 7 0.08304858577920982 2207 8 0.21611471908759938 2207 9 0.20577433612576357 2207 10 0.5977431309998593 2207 11 0.4103599861328959 2207 12 0.5296974372819179 2207 13 0.5713940678075602 2207 14 0.4921924136916971 2207 15 0.5089768151901595 2207 16 0.1532448489191509 2207 17 0.27022962571065534 2207 18 0.4369906332252995 2207 19 0.6125569603050067 2207 20 0.3797685394980661 2207 21 0.6701382667069353 2207 22 0.6977231097310721 2207 23 0.6176763144117103 2207 24 0.7698866041867234 2207 25 0.9262534496798978 2208 1 0.05712642434281534 2208 2 0.09898714919247098 2208 3 -0.08638235576067999 2208 4 -0.10120991192159323 2208 5 -0.046285298049028256 2208 6 0.0914040544682777 2208 7 0.43131094794365765 2208 8 0.0949276365289009 2208 9 0.08114542238303102 2208 10 0.20825945486053854 2208 11 -0.18190745172429956 2208 12 -0.17803119021534997 2208 13 0.18529615415335599 2208 14 -0.4002589688443834 2208 15 0.016440837775029138 2208 16 0.188557308957904 2208 17 0.22825777809948278 2208 18 0.05131372664362743 2208 19 0.426578069678321 2208 20 0.12236796307249875 2208 21 0.6774439198089084 2208 22 0.6007527867950415 2208 23 0.6909935156983451 2208 24 0.6164423138492268 2208 25 0.5388246421725924 2209 1 -0.09738637613737922 2209 2 -0.09952557506603554 2209 3 -0.0386418525069665 2209 4 -0.7576577905766655 2209 5 -0.04539159872454235 2209 6 -0.23509918018972797 2209 7 0.006861887852387838 2209 8 -0.0034650385666168404 2209 9 0.04449517513891976 2209 10 0.07184263654445999 2209 11 0.005011953365404054 2209 12 -0.14101793716886846 2209 13 0.0389571772389985 2209 14 0.1656251551302006 2209 15 -0.3309023599154557 2209 16 -0.5230703099717502 2209 17 0.1308683710089582 2209 18 -0.18418176198505165 2209 19 0.15383399353284943 2209 20 0.11861595034347516 2209 21 0.5407317424410394 2209 22 0.4694294829658574 2209 23 0.44510710507304196 2209 24 0.5250153485205686 2209 25 0.47775963459805837 2210 1 0.052310418204768894 2210 2 0.06939959841094989 2210 3 0.09864533430729239 2210 4 0.0942790169695229 2210 5 0.05578334184237832 2210 6 -0.3485325977385685 2210 7 -0.0952695499857299 2210 8 -0.09391567604917839 2210 9 0.03454839894238404 2210 10 0.35559520800087796 2210 11 0.33133263050979167 2210 12 0.3301027057722905 2210 13 0.360654452812625 2210 14 0.35338795573829884 2210 15 0.2708815770949258 2210 16 0.4541567030224911 2210 17 -1.0266295434889372 2210 18 -1.620760126653117 2210 19 -0.24965175296753728 2210 20 -0.04880288907335739 2210 21 -0.1353328579131272 2210 22 -0.028720659265886184 2210 23 0.035102859497833094 2210 24 0.37576299729715856 2210 25 0.5179454456272126 2211 1 -0.32756271513737545 2211 2 -0.4950469017432262 2211 3 -0.14233523923572378 2211 4 -0.0994128908759464 2211 5 -0.2743783250445446 2211 6 -0.14257250787468934 2211 7 -0.36831919537580404 2211 8 -0.4881494102459101 2211 9 -0.395344557315056 2211 10 -0.18733241633285758 2211 11 -0.34112673251386383 2211 12 -0.1794563444898074 2211 13 -0.16626066105901316 2211 14 -0.3314937675338001 2211 15 -0.16352659875636277 2211 16 -0.15244653031524535 2211 17 -0.42564280281671785 2211 18 -0.3738767294459464 2211 19 -0.21216441760785898 2211 20 -0.450349076465983 2211 21 -0.10288935322170545 2211 22 -0.1041578646753436 2211 23 -0.7816893522990043 2211 24 -0.2756620500575835 2211 25 -1.0561702320962354 2212 1 0.058815175489208116 2212 2 -0.06921014585407192 2212 3 0.02491815128632524 2212 4 0.26732998245239514 2212 5 0.2894430681211375 2212 6 0.17451127224548912 2212 7 -0.013658112701691039 2212 8 -0.016865159397859486 2212 9 0.2558784584455651 2212 10 -0.10513038100803129 2212 11 0.23220026150425355 2212 12 0.041107989443737836 2212 13 -0.0314659132491073 2212 14 -0.1508940952898854 2212 15 0.19393996809981218 2212 16 0.09002890483827741 2212 17 -0.22995353716569156 2212 18 -0.023774558567680515 2212 19 -0.09459035215105871 2212 20 -0.3053035210677919 2212 21 0.1333951336646848 2212 22 -0.3267184085076593 2212 23 -0.08828291452208248 2212 24 -0.2180147452781469 2212 25 0.2153075701845708 2213 1 -0.14055841508959688 2213 2 -0.13748996592542184 2213 3 -0.019178685644387627 2213 4 0.20813608189041588 2213 5 -0.05275067732993932 2213 6 -0.27910698788742394 2213 7 -0.13608476314136908 2213 8 -0.22070236453799197 2213 9 -0.06326938610944666 2213 10 0.13173112881764837 2213 11 0.21464647978108786 2213 12 0.2555333035433165 2213 13 0.33940903156720026 2213 14 -0.7482798799720882 2213 15 -0.2500053374090143 2213 16 -0.2937429150898936 2213 17 -0.3500415924329019 2213 18 -0.23344035422696408 2213 19 -0.0227871282305685 2213 20 -0.08280647414077436 2213 21 -0.12407107005559226 2213 22 -0.1289388807607068 2213 23 -0.42359092898318806 2213 24 -0.40080936650925025 2213 25 -0.22181762817841388 2214 1 -0.22337347237273875 2214 2 -0.014997564709616995 2214 3 0.009486266424818979 2214 4 0.255575876138721 2214 5 -0.062149788856707744 2214 6 0.31600640935362634 2214 7 -0.35778221462605586 2214 8 0.0423558538393749 2214 9 -0.06626213803836833 2214 10 0.0316843670687561 2214 11 0.055750057054879676 2214 12 0.17133877466793734 2214 13 0.2309685358926643 2214 14 0.37365138025704425 2214 15 0.26287752441001544 2214 16 -0.07704897073637085 2214 17 -0.2118372435902529 2214 18 0.04935132218173521 2214 19 -0.20740251284595246 2214 20 -0.13005922139352322 2214 21 -0.24546457017250134 2214 22 -0.34553930161644075 2214 23 -0.5187978497220271 2214 24 -0.639434571091904 2214 25 -0.7828650913535355 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 2.0 10 11.0 11 33.0 12 121.0 13 321.0 14 757.0 15 1563.0 16 2609.0 17 3392.0 18 3832.0 19 3900.0 20 3749.0 21 3624.0 22 3515.0 23 3516.0 24 3661.0 25 4012.0 26 4448.0 27 4651.0 28 5346.0 29 6748.0 30 8574.0 31 10971.0 32 14913.0 33 20390.0 34 27842.0 35 39069.0 36 56315.0 37 90245.0 38 160642.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.954636219343605 18.460498694383286 10.42486303824689 21.160002048026215 2 14.358374217941217 24.0018904442544 34.69578987425682 26.94394546354757 3 22.621291205414572 32.16787800821035 26.04759875779451 19.16323202858056 4 12.393751764835875 21.95987837467312 30.734949275036115 34.91142058545489 5 10.786657116545586 41.71636140386779 31.86247825642075 15.634503223165867 6 29.727611650167585 32.5585107036017 18.922723719803404 18.791153926427317 7 22.698279196262487 37.44022002713504 20.48327388589776 19.378226890704713 8 28.600281985851595 31.787458791799082 22.030707399775313 17.58155182257401 9 29.105271556997714 14.927144242533357 19.866645934521884 36.10093826594704 10 19.921245124037902 30.802934101847267 27.244380447263605 22.03144032685122 11 29.204730180698473 27.924731490997836 17.4308714719166 25.439666856387095 12 24.909312988344187 31.860858606008158 27.503585538362703 15.726242867284954 13 33.700851518288616 21.265656520191335 23.444577657567713 21.58891430395234 14 23.489876587665314 27.95293472567762 27.580365326698963 20.9768233599581 15 33.8761981729461 21.49335570263828 25.8387977862572 18.79164833815842 16 17.369003606991384 27.16823556129328 33.28068448952786 22.18207634218748 17 23.490406609189524 27.417170307749593 27.946658373808752 21.14576470925213 18 24.14285538919828 19.441295977281932 34.15259404141791 22.263254592101873 19 21.007179231173698 27.8478060277717 35.572077721468744 15.572937019585858 20 23.193745984851894 20.178855667445486 35.331461984475446 21.295936363227177 21 19.65083996047245 24.839342598711468 36.75366683511569 18.756150605700398 22 21.747661409783205 24.506502221967608 28.85100131763088 24.894835050618305 23 18.8962588026368 24.316964486654363 34.54602740734982 22.240749303359017 24 20.931146092058906 28.598902560283484 31.244092361705 19.225858985952605 25 21.54722470583662 22.232429479559457 30.92546633412377 25.294879480480155 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1625.0 1 1625.0 2 1736.0 3 1847.0 4 1847.0 5 1847.0 6 4508.0 7 7169.0 8 7169.0 9 7169.0 10 6478.5 11 5788.0 12 5788.0 13 5788.0 14 3999.5 15 2211.0 16 2211.0 17 2211.0 18 3721.0 19 5231.0 20 5231.0 21 5231.0 22 8493.5 23 11756.0 24 11756.0 25 11756.0 26 17223.0 27 22690.0 28 22690.0 29 22690.0 30 25330.0 31 27970.0 32 27970.0 33 27970.0 34 29867.5 35 31765.0 36 31765.0 37 31765.0 38 24803.5 39 17842.0 40 17842.0 41 17842.0 42 26629.0 43 35416.0 44 35416.0 45 35416.0 46 79965.5 47 124515.0 48 124515.0 49 124515.0 50 108936.5 51 93358.0 52 93358.0 53 93358.0 54 68365.0 55 43372.0 56 43372.0 57 43372.0 58 32497.5 59 21623.0 60 21623.0 61 21623.0 62 20913.0 63 20203.0 64 20203.0 65 20203.0 66 14110.0 67 8017.0 68 8017.0 69 8017.0 70 6205.0 71 4393.0 72 4393.0 73 4393.0 74 2954.5 75 1516.0 76 1516.0 77 1516.0 78 954.5 79 393.0 80 393.0 81 393.0 82 224.0 83 55.0 84 55.0 85 55.0 86 34.5 87 14.0 88 14.0 89 14.0 90 9.5 91 5.0 92 5.0 93 5.0 94 2.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10209217347894937 2 0.0 3 0.1248020557558299 4 0.012275612041557037 5 0.02536959821921788 6 0.012275612041557037 7 0.02189150814077672 8 0.01984557280051721 9 0.0 10 0.3983436107485259 11 0.06874342743271943 12 0.0010229676701297532 13 0.0 14 0.001227561204155704 15 0.0018413418062335559 16 2.0459353402595064E-4 17 0.0 18 0.0 19 6.13780602077852E-4 20 0.0 21 2.0459353402595064E-4 22 0.0036826836124671117 23 0.0 24 0.0 25 0.0030689030103892593 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 488774.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.025819703994074 #Duplication Level Percentage of deduplicated Percentage of total 1 81.75539685945178 16.372188373358647 2 9.049764509966183 3.6245790488037417 3 3.0720977513511305 1.845638270448101 4 1.4813906682604385 1.1866424973505139 5 0.8346870179095023 0.8357645864960083 6 0.5414738304676087 0.6506074382025231 7 0.3882265199579081 0.5442188005090287 8 0.2738018614439983 0.4386485369516382 9 0.26767196902361035 0.48243155323319165 >10 1.6836771181332435 6.612258426184699 >50 0.20841634229319278 3.0314623936625105 >100 0.3289708932274905 14.097517462058129 >500 0.04495254441617883 6.156219438840855 >1k 0.05925562673041755 22.314607569142385 >5k 0.005108243683656685 6.798233948614288 >10k+ 0.005108243683656685 15.008981656143739 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 18562 3.7976651785896958 No Hit GTACATGGGAAGCAGTGGTATCAAC 16436 3.362699325250525 No Hit GTATCAACGCAGAGTACTTTTTTTT 16001 3.2737011379492365 No Hit GGTATCAACGCAGAGTACTTTTTTT 12034 2.46207858846829 No Hit TATCAACGCAGAGTACTTTTTTTTT 10327 2.112837425885992 No Hit GAGTACATGGGAAGCAGTGGTATCA 8271 1.6921931199286377 No Hit CATGTACTCTGCGTTGATACCACTG 7554 1.5454995560320313 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 6667 1.364025091351013 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 5436 1.1121704509650676 No Hit GCGTTGATACCACTGCTTCCCATGT 5300 1.0843457303375386 No Hit ACGCAGAGTACATGGGAAGCAGTGG 4899 1.0023037231931322 No Hit GTATCAACGCAGAGTACATGGGAAG 4284 0.8764786997671725 No Hit TATCAACGCAGAGTACATGGGAAGC 4013 0.8210338520461399 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 3978 0.8138730783552317 No Hit ACTCTGCGTTGATACCACTGCTTCC 3719 0.7608833530425104 No Hit GTACTGGTTCACTATCGGTCAGTCA 3578 0.7320356647448514 No Hit GGTATCAACGCAGAGTACATGGGAA 3323 0.679864313568234 No Hit GGATACCACGTGTCCCGCCCTACTC 2736 0.559767909095001 No Hit GTTAATGATAGTGTGTCGAAACACA 2708 0.5540392901422744 No Hit GCTTCCCATGTACTCTGCGTTGATA 2623 0.5366488397500685 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 2453 0.5018679389656568 No Hit CAGTGGTATCAACGCAGAGTACATG 2321 0.47486159247423143 No Hit GTGGTATCAACGCAGAGTACATGGG 2200 0.45010577485709136 No Hit ATACAGGGTGACAGCCCCGTACACA 2077 0.42494077017189946 No Hit GGGAAGCAGTGGTATCAACGCAGAG 2018 0.4128697516643684 No Hit ACTTAGATGTTTCAGTTCCCCCGGT 1989 0.40693653917761585 No Hit CATGGGAAGCAGTGGTATCAACGCA 1964 0.40182170082696705 No Hit GCAGTGGTATCAACGCAGAGTACAT 1874 0.3834082827646315 No Hit GCAGAGTACATGGGAAGCAGTGGTA 1873 0.38320368923060555 No Hit ATACCACTGCTTCCCATGTACTCTG 1830 0.37440616726748965 No Hit AGCGTACACGGTGGATGCCCTGGCA 1783 0.36479027116827 No Hit GTACTCTGCGTTGATACCACTGCTT 1766 0.3613121810898289 No Hit ACCCTGTATCGCGCGCCTTTCCAGA 1760 0.36008461988567314 No Hit ATCAACGCAGAGTACTTTTTTTTTT 1685 0.34474010483372686 No Hit CCCATATTCAGACAGGATACCACGT 1645 0.3365563634726888 No Hit ACATGGGAAGCAGTGGTATCAACGC 1632 0.3338966475303515 No Hit CTGCTTCCCATGTACTCTGCGTTGA 1608 0.32898640271372864 No Hit GTGGATGCCCTGGCAGTCAGAGGCG 1598 0.32694046737346916 No Hit AAGCAGTGGTATCAACGCAGAGTAC 1561 0.31937050661450894 No Hit ATCATTAACTGAATCCATAGGTTAA 1561 0.31937050661450894 No Hit GTACATGGGGGTTAAGCGACTAAGC 1551 0.31732457127424946 No Hit CTATCGGTCAGTCAGGAGTATTTAG 1547 0.31650619713814565 No Hit TACCACTGCTTCCCATGTACTCTGC 1500 0.30689030103892595 No Hit TCATTAACCTATGGATTCAGTTAAT 1469 0.30054790148412147 No Hit CCTATGGATTCAGTTAATGATAGTG 1409 0.28827228944256444 No Hit ATGGGAAGCAGTGGTATCAACGCAG 1404 0.28724932177243473 No Hit GTATTTAGCCTTGGAGGATGGTCCC 1387 0.28377123169399354 No Hit GTTGATACCACTGCTTCCCATGTAC 1380 0.2823390769558119 No Hit GATACCACTGCTTCCCATGTACTCT 1376 0.2815207028197081 No Hit CCATGTACTCTGCGTTGATACCACT 1369 0.28008854808152644 No Hit CCACTGCTTCCCATGTACTCTGCGT 1334 0.27292777439061816 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 1319 0.2698588713802289 No Hit GTCAGGAGTATTTAGCCTTGGAGGA 1306 0.2671991554378915 No Hit GTACATGGTAAGCAGTGGTATCAAC 1296 0.26515322009763204 No Hit GTTCACTATCGGTCAGTCAGGAGTA 1285 0.2629026912233466 No Hit GCCTTGGAGGATGGTCCCCCCATAT 1275 0.2608567558830871 No Hit GCCCAGAGCCTGAATCAGTGTGTGT 1274 0.26065216234906113 No Hit GTGGTATCAACGCAGAGTACTTTTT 1215 0.24858114384153002 No Hit GTACTTAGATGTTTCAGTTCCCCCG 1213 0.24817195677347814 No Hit CTTCCCATGTACTCTGCGTTGATAC 1213 0.24817195677347814 No Hit GGTATCAACGCAGAGTACATGGGGG 1197 0.24489846022906292 No Hit TATTCAGACAGGATACCACGTGTCC 1146 0.23446418999373944 No Hit GGTTAATGAGGCGAACCGGGGGAAC 1128 0.23078150638127232 No Hit GCCTGAATCAGTGTGTGTGTTAGTG 1117 0.22853097750698687 No Hit GTGTGTGTGTTAGTGGAAGCGTCTG 1106 0.22628044863270141 No Hit ACCATGTACTCTGCGTTGATACCAC 1095 0.22402991975841594 No Hit GTATCAACGCAGAGTACATGGGGGT 1088 0.2225977650202343 No Hit CGGTACTGGTTCACTATCGGTCAGT 1010 0.20663946936621014 No Hit CACACACACTGATTCAGGCTCTGGG 977 0.19988788274335378 No Hit GGGTACTTAGATGTTTCAGTTCCCC 959 0.1962051991308867 No Hit ACGCAGAGTACATGGGGGTTAAGCG 887 0.18147446468101824 No Hit GAATCAGTGTGTGTGTTAGTGGAAG 878 0.17963312287478467 No Hit GTGCTAATCTGCGATAAGCGTCGGT 868 0.17758718753452515 No Hit ATGATAGTGTGTCGAAACACACTGG 853 0.1745182845241359 No Hit CATCTAAGTACCCCGAGGAAAAGAA 798 0.1632656401527086 No Hit GAGTACATGGGGGTTAAGCGACTAA 789 0.16142429834647504 No Hit ATCAACGCAGAGTACATGGGAAGCA 760 0.1554910858597225 No Hit TCACGGTACTGGTTCACTATCGGTC 760 0.1554910858597225 No Hit TCTAAGTACCCCGAGGAAAAGAAAT 759 0.15528649232569655 No Hit GGTTCACTATCGGTCAGTCAGGAGT 748 0.15303596345141107 No Hit TAATGATAGTGTGTCGAAACACACT 732 0.14976246690699585 No Hit GCGTACACGGTGGATGCCCTGGCAG 725 0.14833031216881423 No Hit GTACATGGGTAAGCAGTGGTATCAA 717 0.14669356389660662 No Hit CAGTTAATGATAGTGTGTCGAAACA 716 0.14648897036258066 No Hit GGAGTATTTAGCCTTGGAGGATGGT 714 0.14607978329452875 No Hit GCCCTACTCATCGAGCTCACAGCAT 710 0.14526140915842495 No Hit TGATACCACTGCTTCCCATGTACTC 707 0.1446476285563471 No Hit TTCCCATGTACTCTGCGTTGATACC 703 0.1438292544202433 No Hit CATTAACCTATGGATTCAGTTAATG 697 0.1426016932160876 No Hit GGTAAGCAGTGGTATCAACGCAGAG 688 0.14076035140985405 No Hit GTGTTAGTGGAAGCGTCTGGAAAGG 667 0.13646388719530908 No Hit GGGTTTCCCCATTCGGAAATCGCCG 657 0.13441795185504957 No Hit GTCCCGCCCTACTCATCGAGCTCAC 648 0.13257661004881602 No Hit GATATGAACCGTTATAACCGGCGAT 645 0.13196282944673818 No Hit CAACGCAGAGTACTTTTTTTTTTTT 642 0.1313490488446603 No Hit GTTATAACCGGCGATTTCCGAATGG 635 0.12991689410647864 No Hit CCCCATGTACTCTGCGTTGATACCA 629 0.12868933290232296 No Hit GGTGATATGAACCGTTATAACCGGC 624 0.1276663652321932 No Hit AGCTCACAGCATGTGCATTTTTGTG 619 0.12664339756206344 No Hit TATCAACGCAGAGTACATGGGGGTT 605 0.12377908808570014 No Hit GGAAGCAGTGGTATCAACGCAGAGT 597 0.12214233981349254 No Hit CCCCAGTAGCGGCGAGCGAACGGGG 590 0.12071018507531087 No Hit CACTATCATTAACTGAATCCATAGG 573 0.11723209499686972 No Hit AGTCAGGAGTATTTAGCCTTGGAGG 562 0.11498156612258426 No Hit CCCTGGCAGTCAGAGGCGATGAAGG 547 0.11191266311219501 No Hit GAGTACATGGTAAGCAGTGGTATCA 544 0.11129888251011714 No Hit GCTCTGGGCTGCTCCCCGTTCGCTC 535 0.1094575407038836 No Hit AACGCAGAGTACTTTTTTTTTTTTT 535 0.1094575407038836 No Hit GGGTGACAGCCCCGTACACAAAAAT 530 0.10843457303375385 No Hit CACTGATTCAGGCTCTGGGCTGCTC 529 0.10822997949972789 No Hit GATTCAGGCTCTGGGCTGCTCCCCG 527 0.107820792431676 No Hit CCGTTATAACCGGCGATTTCCGAAT 505 0.10331973468310507 No Hit TCGGTAAGGTGATATGAACCGTTAT 495 0.10127379934284557 No Hit GTGTGTTAGTGGAAGCGTCTGGAAA 494 0.10106920580881962 No Hit AAAAAGTACTCTGCGTTGATACCAC 491 0.10045542520674176 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCACAG 25 0.005955067 19.039133 5 GTACAAA 50 2.1376582E-7 19.023502 1 GAAATCG 105 0.0 16.255812 16 AATCGCC 100 1.8189894E-12 16.120344 18 GAGCCCC 55 1.1044842E-5 15.575873 9 ATCGCCG 105 5.456968E-12 15.352711 19 CGCAGAA 45 6.739805E-4 14.780889 2 TACAAAA 125 0.0 14.443039 2 GGGAGCC 60 2.5119085E-5 14.27935 7 CAAGCAG 40 0.0052042804 14.2778845 9 AGAACAA 120 3.6379788E-12 14.252998 2 ACAACCC 40 0.0052971975 14.239861 11 AAATCGC 115 2.5465852E-11 14.017691 17 GCAGAAA 55 1.9523277E-4 13.821089 3 GACCGAT 60 4.1469064E-4 12.643408 18 TGACCGA 60 4.1469064E-4 12.643408 17 CAGGGTA 55 0.0030164008 12.115811 4 CTGACCG 55 0.0031057277 12.0687065 16 ACTCCTG 110 3.893365E-8 12.067472 15 AAAAGTA 90 7.4137424E-6 11.613555 2 >>END_MODULE