FastQCFastQC Report
Wed 25 May 2016
SRR1294676_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294676_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3390595
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT175270.5169299193799318No Hit
GTACATGGGAAGCAGTGGTATCAAC143860.4242913117019284No Hit
CCCATGTACTCTGCGTTGATACCAC133980.3951518833715026No Hit
GGTATCAACGCAGAGTACTTTTTTT106110.31295392106695136No Hit
ATTCCATTCCATTCCATTCCATTCC99990.2949039917772544No Hit
TATCAACGCAGAGTACTTTTTTTTT98590.2907749229854937No Hit
GAATGGAATGGAATGGAATGGAATG73630.21715952509810227No Hit
GAGTACATGGGAAGCAGTGGTATCA65930.1944496467434182No Hit
GTACTGGTTCACTATCGGTCAGTCA60260.1777269181367872No Hit
CATGTACTCTGCGTTGATACCACTG53630.15817282807294883No Hit
ACGCAGAGTACTTTTTTTTTTTTTT48140.14198097973954424No Hit
GTTAATGATAGTGTGTCGAAACACA46360.13673116370430557No Hit
GTATCAACGCAGAGTACATGGGAAG42370.12496331764778748No Hit
GCGTTGATACCACTGCTTCCCATGT42040.12399003714687244No Hit
GGATACCACGTGTCCCGCCCTACTC39690.11705910024641691No Hit
GGTATCAACGCAGAGTACATGGGAA38060.11225168443886693No Hit
ACGCAGAGTACATGGGAAGCAGTGG34940.103049759702943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCC2400.014.2574118
GTCGTAT400.00528224914.2494131
GGACCGT802.0066327E-613.0594556
ATCGCCG2550.013.03713919
AAATCGC2700.012.67194417
TGTCCCG7850.012.09766211
CGGAAAT3150.012.06833914
GAAATCG3000.011.72154816
GTCCCGC8200.011.7052312
ACCGCTC658.0464093E-411.6890658
CGCCCTA8500.011.516561516
GTAGGAC1604.5474735E-1111.2782883
TATACCG855.339586E-511.1738665
CCGCCCT8900.011.10574815
AGTGCGA600.00589087811.08009610
TCCCGCC8750.011.07873513
CCCGCCC8850.011.06094114
TGGACCG909.544283E-510.5530965
GTACTGG16100.010.38466551
GTGTCCC9450.010.25097410
Produced by FastQC (version 0.11.5)